• The Korea Journal of Herbology
• /
• v.38 no.5
• /
• pp.39-47
• /
• 2023

Objectives : The objective of present study was to investigate the vasorelaxant effects of 20 Korean native plants on isolated rat thoracic aorta precontracted with phenylephrine (PE). Methods : Dried 20 plant materials were extracted 3 times with water, ethanol, or methanol for 3h in the reflux apparatus at 70 ± 5℃. Male SD rats were anesthetized by ether inhalation, and their aorta rings were isolated and placed in 10 ㎖ Krebs Henseleit (KH) buffer. While using an isolated organ-chamber technique, the aorta rings were maintained by bubbling with a gas mixture of 95% O₂-5% CO₂ at 37℃. Changes in isometric tension of aorta rings were recorded via isometric transducers connected to a Powerlab Data Acquisition System. Results : Among the 20 native plants, Chrysanthemum indicum L. flower, Nelumbo nucifera Gaertn. rhizome, Schisandra chinensis (Turcz.) Baill. fruit, Anthriscus sylvestris (L.) Hoffm. root, Corydalis turtschaninovii Besser tuber, Corydalis decumbens (Thunb.) Pers. tuber, and Dolichos lablab L. seed showed significant vasorelaxant effect on the contraction of aorta rings induced by PE. In contrast, Mertensia maritima subsp. asiatica Takeda whole plant, Ajuga decumbens Thunb. whole plant, Trigonotis peduncularis (Trevis.) Benth. ex Baker & S.Moore whole plant, Dioscorea quinquelobate Thunb. rhizome, Allium microdictyon Prokh aerial part, Momordica charantia L. fruit, Carthamus tinctorius L. flower, and Clematis terniflora DC. root constricted more the aorta rings precontracted by PE Conclusion : These results suggest that the possibility as useful herbal resources for the development of functional foods or medicines for hypertension treatment.

• Korean Journal of Plant Resources
• /
• v.20 no.6
• /
• pp.563-569
• /
• 2007

RAPD analysis showed that all the OTUs of 'Sandolbae' were the same species because amplified band patterns of all samples generated by each of 5 random primers were identical. Even though there were different environmental conditions, all the "Chuiangne" trees from three different places were the same species, and also all the "Cheongshilli" trees were the same species too. No genetic variations were detected between native Korean pears grown in the habitats and in the research field. Because 212 polymorphic bands were generated by 9 primers selected through primer screening, they were possible to analyze genetic relationship among naturally growing three native Korean pears and nine cultivars of Pyrus pyrifolia and P. communis. Based on the RAPD analysis, three main groups were formed. The first group represented the Six P. pyrifoia cultivars, the second group was the three native Korean pears, and the last group was the three P. communis cultivars. Genetic distance between 'Wonwhang' and 'Chojuro' was closer than other cultivars in group 1 since dissimilarity index value between these two cultivars was 50.82. However, genetic distance between 'Niitaka' and 'Chojuro' was the most distant compared to the others in group 1. In group 2, 'Sandlobae' was genetically closer to 'Chuiangne' than 'Cheongshilli' because dissimilarity index value between 'Sandlobae' and 'Chuiangne' was smaller, 50.82, than the value between 'Sandlobae' and 'Cheongshilli', 63.636. In group 3, 'Old Home' was genetically closer to 'Bartlett' than 'Kaiser Alexander(or Bosc)'. Group 3 composed of P. communis cultivars was genetically further than other two groups, P. pyrifolia cultivars and native Korean pears.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.13 no.1
• /
• pp.112-120
• /
• 2010

This study was carried out to develop the environmental friendly restoration technique for various disturbed slopes using native plants seed chips. As the result of the separated seeding using the developed seed chips of native plant combination, average germination rate and survival rate of trees, shrubs, and herbs were higher than those of the direct seeding, as 79~70%, 83~73%, and 59~53% or so, respectively. Also, the germination rates and the survival rates of trees, shrubs, and herbs in the separated seeding by the native plants seed chips were 10~15 times higher than those of the direct seeding. Numbers of plants survived were at least 10 times of that of the simple seeding. The native plants seed chip technique is more efficient in soil slopes compared with stone slopes. The application of the native plants seed chip technique makes the restoration stabilized through the herbs dominant vegetation to the woody dominant 1 within 3 years, and the Landscapes also changed from herbs to variously combined herbs and woody. The technique using native plants seed chips that can be applicable to various disturbed slops is more efficient than conventional restoration techniques in the respect of economics and landscape.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.32 no.2
• /
• pp.120-129
• /
• 2004

This study was carried out to develop new varieties with early maturing and large fruit size in the Korean native dogwood (Cornus kousa) for woody landscape plants from native sites or cultivated area. The results are obtained as follows; We selected strains with early maturing and large fruit. size from the habitats from 2000 to 2003. ‘SKK 2-1’ had 4.0cm of fruit size while 2.0 cm of native variety. In addition, ‘SKK 2-2’, ‘SKK 2-3’, ‘SKK 2-4’, and ‘SKK 2-5’ with large fruit and early maturing were preliminary selected from the habitats. In the preliminary selections, ‘SKK 2-1’ was finally selected as new early maturing variety with large fruits in 2003. New early maturing variety with large fruit size, C. kousa var. ‘SKK 2-1’ was selected from 200 plants of 5-year-old seedlings in C. kousa in 5-12 Nung wean ri Goo sung myun Yong In, Kyung gi do, Korea in 2000. Their fruits were colorized in September 18, 20∼25 days earlier than October 7 of native varieties. Its fruit diameter is larger than 4cm. Its fruit weight was 5 times as heavy as native cultivars grown in Korea. This useful characters were still inherited after grafting. However, characteristics of growth, leaf, flower, and flowering period were not different from those of native varieties. Therefore, it was very promising landscape woody plant, having large fruits in early fall season. The rooting rate of Korean dogwood was high by the IBA treatment. The optimum date for softwood cutting was on June 25 ∼ July 25 when the rooting rate was more than 80%. In addition. Korean dogwood was easily propagated by grafting. Therefore, new early maturing variety with large fruits, C. kousa var. ‘SKK 2-1’ is easily propagated by softwood cutting or grafting and then is a promising cultivar as a woody landscape plant.

• Journal of Korean Society of Rural Planning
• /
• v.12 no.4 s.33
• /
• pp.83-88
• /
• 2006

Recently, management and restoration efforts using the plants promote the disturbed habitats such as a set-asides or field boundaries. But, side effects are coming out because of using the exotic plants in restoration process. This study was conducted to select the native plant species used for restoration through the vegetation survey and plant community analysis at small stream-side in rural villages. We surveyed at two small stream-sides in open field and high land area in 2005 as a case study. Total 126 species was found at small stream side in open field area. As the result of important value' calculation, Persicaria thunbergii 23.0%, Humulus japonicus 18.6%, Phragmites communis 4.4%, Bromus japonicus 4.2%, and Rosa multiflora 3.4% were in the order of important value. Total 92 species were found at small stream side in high land area. As the result of important value' calculation, Dactylis glomerata 16.1%, Artemisia princeps var. orientalis 11.0%, Persicaria thunbergii 10.9%, Humulus japonicus 9.3%, Phragmites japonica 5.2% and Phragmites communis 3.5% were in the order of important value. We selected some plant species to use in restoration after due consideration of problems of exotic plant and high coverage and density species, and ecological process; Persicaria thunberii, Phragmites communis, Bromus japonicus, Rosa multiflora, Equisetum arvense, Digtaria sanguinalis, Impatiens textori, and Artemisia princeps var. orientalis in open field stream-side, and Artemisia princeps var. orientalis, Persicaria thunbergii, Phragmites japonica, Phragmites communis, Artemisia selengensis, Panicum bisulcatum, Rorippa indica, and Equisetum arvense at hghland stream-side. We will verify the selected native plants and plan the utilization of the native plants in restoration at stream-side in rural village.

• Microbiology and Biotechnology Letters
• /
• v.35 no.3
• /
• pp.210-219
• /
• 2007

In this study was investigated the growth effect of native plant (Microlepia marginata(panzer Christ., Prunella vulgaris. aleutica Fernald, Perillafrutescens var. japonica Hara., Gleichenia japonica Spreng) extracts on various lactic acid bacterias, electron donating ability, total cell count and hydroxyl radical scavenging activity. The total cell count of Enterococcus faecium KCCM 12118, Lactobacillus rhamnosus KCCM 32826, Lactobacillus plantarum KCCM 11542, Pediococcus pentosaceus KCCM 40464 in the absence of native plant extracts (10%) at $37^{\circ}C$ after 48hr were $2.2{\times}10^9\;cfu/ml$, $2.1{\times}10^9\;cfu/ml$, $2.3{\times}10^9\;cfu/ml$, $2.2{\times}10^9\;cfu/ml$. On the other hand, the total cell count of E. faecium KCCM 12118, L. rhamnosus KCCM 32826, L. plantarum KCCM 11542, P. pentosaceus KCCM 40464 in the presence of native plant extracts (10%) at $37^{\circ}C$ after 48hr were $4.3{\times}10^9\;cfu/ml$, $4.3{\times}10^9\;cfu/ml$, $4.8{\times}10^9\;cfu/ml$, $3.9{\times}10^9\;cfu/ml$. The electron donating ability indicated to E. faecium KCCM 12118, L. rhamnosus KCCM 32826, L. plantarum KCCM 11542, P. pentosaceus KCCM 40464 added by 10% native plant extracts, respectively. when 10% native plant extracts were added lactic acid bacterias, the electron donating ability is the highest. Hydroxyl radical scavenging activity of E. faecium KCCM 12118, L. rhamnosus KCCM 32826, L. plantarum KCCM 11542, P. pentosaceus KCCM 40464 showed higher than that of control.

• The Korean Journal of Ecology
• /
• v.22 no.1
• /
• pp.51-58
• /
• 1999

Korean native plants and naturalized plants were analyzed for allelochemicals, and their antimicrobial effects were studied. The difference in soluble solid contents between Korean native plants and naturalized species was not significant, and the Korean native plant, Solanum nigrum showed the highest soluble solid content of 90 mg/ml. The ethanol extract of the Korean native plant, Solanum nigrum showed antifungal activity to Aspergillus phoenicis KCTC 1228, with a clear zone of 18 mm, and spore formation was not observed from the treatment. The naturalized plants Ambrosia artemisiifolia var. elatior and Erigeron canadensis showed inhibition of spore formation and the clear zones were at 24 mm and 22 mm, respectively. The clear zones of Aspergillus phoenicis KCTC 1228 treated with ethanol extrats of Phytolacca americana and Rudbeckia bicolor were 22 mm and 19 mm, respectively, and spore formation was observed from the treatment. The Korean native plant, Solanum nigrum and naturalized plants, Phytolacca americana and Ambrosia artemisiifolia var. elatior showed antimicrobial activity against Bacillus sphiaericus 2362, and Bacillus sphiaericus 2297, Bacillus thuringiensis var. subtilis and Baicillus thuringiensis var. cereus. The antimicrobial activity of Ambrosia artemisiifolia var. elatior showed the largest clear zone of 32 mm against Bacillus thuringiensis var. subtilis. In general, the more soluble the solid contents of the extracts, the greater were the antifungal and antimicrobial activities. The phenolic compounds from the Korean native plant, Solanum nigrum and the naturalized species, Phytolacca americana and Ambrosia artemisiifolia var. elatior were analyzed by high performance liquid chromatography. Three phenolic compounds including hydroquinone were identified in Solanum nigrum. In contrast, five and seven phenolic compounds were identified in Phytolacca americana and Ambrosia artemisiifolia var. elatior, respectively. The antifungal activity against Aspergillus phoenicis KCTC 1228 was found to be due to the coumaric and benzoic compounds.

• The Plant Pathology Journal
• /
• v.15 no.5
• /
• pp.308-310
• /
• 1999

A fungus associated with gray blight on tea plant (Camellia sinensis) was identifed as Pestalotiopsis theae based on the mycological characteristics. Mycelial growth on potato dextrose agar and size and shape of conidia of P. theae were similar to those of P. longiseta, but P. theae was different from P. longiseta in the color of three median cells and the number of apical appendages. Artificial inoculation of conidial suspension or mycelial mats on the wounded leaves and shoots of healthy plants induced the same disease, respectively. The Korean native variety was relatively. The Korean native variety was relatively more resistat to P. theae than a Japanese variety‘Yabukita’which has bee recently introduced and planted in large areas of southern parts of Korea. Here, we report the report the first record of gary blight caused by P. theae on tea plant in Korea.

• The Plant Pathology Journal
• /
• v.31 no.4
• /
• pp.323-333
• /
• 2015

As sessile organisms, plants are exposed to persistently changing stresses and have to be able to interpret and respond to them. The stresses, drought, salinity, chemicals, cold and hot temperatures, and various pathogen attacks have interconnected effects on plants, resulting in the disruption of protein homeostasis. Maintenance of proteins in their functional native conformations and preventing aggregation of non-native proteins are important for cell survival under stress. Heat shock proteins (HSPs) functioning as molecular chaperones are the key components responsible for protein folding, assembly, translocation, and degradation under stress conditions and in many normal cellular processes. Plants respond to pathogen invasion using two different innate immune responses mediated by pattern recognition receptors (PRRs) or resistance (R) proteins. HSPs play an indispensable role as molecular chaperones in the quality control of plasma membrane-resident PRRs and intracellular R proteins against potential invaders. Here, we specifically discuss the functional involvement of cytosolic and endoplasmic reticulum (ER) HSPs/chaperones in plant immunity to obtain an integrated understanding of the immune responses in plant cells.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.17 no.2
• /
• pp.41-46
• /
• 1989

Rhododendron micranthum grown over the area of Kyoung Puck, Kang Weon Province is showy and very attractive evergreen broad-leaned shrub of shade resistance. This shrub is considered very useful for exploiting as a garden plant. Environments, growth characteristics and vegetation association in the wild habitat and in the garden planted with this shrub, were investigated to find out some appropriate means of propagation and cultivation of this shrub. The results thus obtained were stated as follows ; 1. Light intensity for the native habitat ranged in the proximity of 9%. The most abundant stand of this shrub occurred in the northen side of mountains, while still some plants were found in the eastern sides also. 2. Soil of the native habitat was acidic (pH 4.4) and infertility. 3. Average elevation of the native habitat was shown to be 230m above the sea level. 4. The plant of the native habitat showed an average of 3.9 new shoots per branch, whereas that of the garden planted (light intensity 100%) showed 1.2, 5% of an average was for sun-burn leaves were noted, while garden Plants(light intensity 100%) showed 90% of them. 5. Other tree species associated with Rhododendron micrathum in the native habitat were primarily Pinus densiflora and secondarily Sorbus alnifolia, hemerocallis aurautiaca, Rubus crataegifolius, and Salix hulteni. 6. Vegetation rate of plant habitats in the Rhododendron growing area was observed to be 0 to 80% involving 27 to 50 species of associated trees in the vegetation for the 10${\times}$10m quadrat area.