• 대한본초학회지
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• 제38권5호
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• pp.39-47
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• 2023

Objectives : The objective of present study was to investigate the vasorelaxant effects of 20 Korean native plants on isolated rat thoracic aorta precontracted with phenylephrine (PE). Methods : Dried 20 plant materials were extracted 3 times with water, ethanol, or methanol for 3h in the reflux apparatus at 70 ± 5℃. Male SD rats were anesthetized by ether inhalation, and their aorta rings were isolated and placed in 10 ㎖ Krebs Henseleit (KH) buffer. While using an isolated organ-chamber technique, the aorta rings were maintained by bubbling with a gas mixture of 95% O₂-5% CO₂ at 37℃. Changes in isometric tension of aorta rings were recorded via isometric transducers connected to a Powerlab Data Acquisition System. Results : Among the 20 native plants, Chrysanthemum indicum L. flower, Nelumbo nucifera Gaertn. rhizome, Schisandra chinensis (Turcz.) Baill. fruit, Anthriscus sylvestris (L.) Hoffm. root, Corydalis turtschaninovii Besser tuber, Corydalis decumbens (Thunb.) Pers. tuber, and Dolichos lablab L. seed showed significant vasorelaxant effect on the contraction of aorta rings induced by PE. In contrast, Mertensia maritima subsp. asiatica Takeda whole plant, Ajuga decumbens Thunb. whole plant, Trigonotis peduncularis (Trevis.) Benth. ex Baker & S.Moore whole plant, Dioscorea quinquelobate Thunb. rhizome, Allium microdictyon Prokh aerial part, Momordica charantia L. fruit, Carthamus tinctorius L. flower, and Clematis terniflora DC. root constricted more the aorta rings precontracted by PE Conclusion : These results suggest that the possibility as useful herbal resources for the development of functional foods or medicines for hypertension treatment.

• 한국자원식물학회지
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• 제20권6호
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• pp.563-569
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• 2007

RAPD analysis showed that all the OTUs of 'Sandolbae' were the same species because amplified band patterns of all samples generated by each of 5 random primers were identical. Even though there were different environmental conditions, all the "Chuiangne" trees from three different places were the same species, and also all the "Cheongshilli" trees were the same species too. No genetic variations were detected between native Korean pears grown in the habitats and in the research field. Because 212 polymorphic bands were generated by 9 primers selected through primer screening, they were possible to analyze genetic relationship among naturally growing three native Korean pears and nine cultivars of Pyrus pyrifolia and P. communis. Based on the RAPD analysis, three main groups were formed. The first group represented the Six P. pyrifoia cultivars, the second group was the three native Korean pears, and the last group was the three P. communis cultivars. Genetic distance between 'Wonwhang' and 'Chojuro' was closer than other cultivars in group 1 since dissimilarity index value between these two cultivars was 50.82. However, genetic distance between 'Niitaka' and 'Chojuro' was the most distant compared to the others in group 1. In group 2, 'Sandlobae' was genetically closer to 'Chuiangne' than 'Cheongshilli' because dissimilarity index value between 'Sandlobae' and 'Chuiangne' was smaller, 50.82, than the value between 'Sandlobae' and 'Cheongshilli', 63.636. In group 3, 'Old Home' was genetically closer to 'Bartlett' than 'Kaiser Alexander(or Bosc)'. Group 3 composed of P. communis cultivars was genetically further than other two groups, P. pyrifolia cultivars and native Korean pears.

• 한국환경복원기술학회지
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• 제13권1호
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• pp.112-120
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• 2010

This study was carried out to develop the environmental friendly restoration technique for various disturbed slopes using native plants seed chips. As the result of the separated seeding using the developed seed chips of native plant combination, average germination rate and survival rate of trees, shrubs, and herbs were higher than those of the direct seeding, as 79~70%, 83~73%, and 59~53% or so, respectively. Also, the germination rates and the survival rates of trees, shrubs, and herbs in the separated seeding by the native plants seed chips were 10~15 times higher than those of the direct seeding. Numbers of plants survived were at least 10 times of that of the simple seeding. The native plants seed chip technique is more efficient in soil slopes compared with stone slopes. The application of the native plants seed chip technique makes the restoration stabilized through the herbs dominant vegetation to the woody dominant 1 within 3 years, and the Landscapes also changed from herbs to variously combined herbs and woody. The technique using native plants seed chips that can be applicable to various disturbed slops is more efficient than conventional restoration techniques in the respect of economics and landscape.

• 한국조경학회지
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• 제32권2호
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• pp.120-129
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• 2004

This study was carried out to develop new varieties with early maturing and large fruit size in the Korean native dogwood (Cornus kousa) for woody landscape plants from native sites or cultivated area. The results are obtained as follows; We selected strains with early maturing and large fruit. size from the habitats from 2000 to 2003. ‘SKK 2-1’ had 4.0cm of fruit size while 2.0 cm of native variety. In addition, ‘SKK 2-2’, ‘SKK 2-3’, ‘SKK 2-4’, and ‘SKK 2-5’ with large fruit and early maturing were preliminary selected from the habitats. In the preliminary selections, ‘SKK 2-1’ was finally selected as new early maturing variety with large fruits in 2003. New early maturing variety with large fruit size, C. kousa var. ‘SKK 2-1’ was selected from 200 plants of 5-year-old seedlings in C. kousa in 5-12 Nung wean ri Goo sung myun Yong In, Kyung gi do, Korea in 2000. Their fruits were colorized in September 18, 20∼25 days earlier than October 7 of native varieties. Its fruit diameter is larger than 4cm. Its fruit weight was 5 times as heavy as native cultivars grown in Korea. This useful characters were still inherited after grafting. However, characteristics of growth, leaf, flower, and flowering period were not different from those of native varieties. Therefore, it was very promising landscape woody plant, having large fruits in early fall season. The rooting rate of Korean dogwood was high by the IBA treatment. The optimum date for softwood cutting was on June 25 ∼ July 25 when the rooting rate was more than 80%. In addition. Korean dogwood was easily propagated by grafting. Therefore, new early maturing variety with large fruits, C. kousa var. ‘SKK 2-1’ is easily propagated by softwood cutting or grafting and then is a promising cultivar as a woody landscape plant.

• 농촌계획
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• 제12권4호
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• pp.83-88
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• 2006

Recently, management and restoration efforts using the plants promote the disturbed habitats such as a set-asides or field boundaries. But, side effects are coming out because of using the exotic plants in restoration process. This study was conducted to select the native plant species used for restoration through the vegetation survey and plant community analysis at small stream-side in rural villages. We surveyed at two small stream-sides in open field and high land area in 2005 as a case study. Total 126 species was found at small stream side in open field area. As the result of important value' calculation, Persicaria thunbergii 23.0%, Humulus japonicus 18.6%, Phragmites communis 4.4%, Bromus japonicus 4.2%, and Rosa multiflora 3.4% were in the order of important value. Total 92 species were found at small stream side in high land area. As the result of important value' calculation, Dactylis glomerata 16.1%, Artemisia princeps var. orientalis 11.0%, Persicaria thunbergii 10.9%, Humulus japonicus 9.3%, Phragmites japonica 5.2% and Phragmites communis 3.5% were in the order of important value. We selected some plant species to use in restoration after due consideration of problems of exotic plant and high coverage and density species, and ecological process; Persicaria thunberii, Phragmites communis, Bromus japonicus, Rosa multiflora, Equisetum arvense, Digtaria sanguinalis, Impatiens textori, and Artemisia princeps var. orientalis in open field stream-side, and Artemisia princeps var. orientalis, Persicaria thunbergii, Phragmites japonica, Phragmites communis, Artemisia selengensis, Panicum bisulcatum, Rorippa indica, and Equisetum arvense at hghland stream-side. We will verify the selected native plants and plan the utilization of the native plants in restoration at stream-side in rural village.

• 한국미생물·생명공학회지
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• 제35권3호
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• pp.210-219
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• 2007

본 연구는 제주도 자생식물 4종(Microlepia marginata panzer Christ., Prunella vulgaris. aleutica Fernald, Perilla-frutescens var. japonica Hara., Gleichenia japonica Spreng)을 부위별로 나누어 각 부위를 열수 추출하여 각 추출액을 농도별로 첨가하여 E. faecitum KCCM 12118, L. rhamnosus KCCM 32826, L. plantarum KCCM 11542, P. pentosaceus KCCM 40464 균종에 대한 생육조건 및 배양액을 가지고 어류 질병 미생물에 대한 항균활성 및 DPPH 라디칼 소거활성 및 Hydroxyl radical 소거활성을 조사하였다. 자생식물 추출액을 농도별로 첨가하여 4종의 lactic acid bacteria를 72시간 배양하면서 생육활성을 보았을 때 10%를 첨가하여 48시간 배양하였을 때가 가장 좋은 생육 조건임을 알 수가 있었으며, 또한 lactic acid bacteria 생육시 자생식물 추출물 첨가가 lactic acid bacteria 증식에 효과가 있음을 나타내었다. DPPH 라디칼 소거활성에서는 꿀풀 잎 추출물을 10% 첨가하여 배양하였을 때 합성 항산화제인 BHA(90%)와 BHT(81%) EDA를 비교하였을 때 BHA와 유사하거나 조금 높은 활성을 BHT보다는 높은 라디칼 소거활성을 나타내었다. Hydroxyl radical 소거활성은 5% 자생식물 추출물을 첨가한 배양액에서부터 BHT보다 높은 소거활성을 나타내기 시작하여 10%를 첨가한 배양액에서는 BHA보다 높은 소거 활성을 나타내었다.

• The Korean Journal of Ecology
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• 제22권1호
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• pp.51-58
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• 1999

자생식물과 귀화식물을 대상으로 allelochemicals를 분석하고 그 항균효과를 조사하였다. 자생식물과 귀화식물 추출액 간의 가용성 고형물 함량에는 차이를 보이지 않았고 자생식물인 까마중의 가용성 고형물 함량이 90 mg/ml로 가장 높게 나타났다. 자생식물의 에탄을 추출액에 의한 Aspergillus phoenicis KCTC 1228의 항균력은 까마중에서만 나타났으며 clear zone이 18 mm였고 포자형성은 억제되었다. 귀화식물 중 돼지풀과 망초에서 포자형성이 억제되면서 clear zone이 24 mm와 22 mm로 형성되었고 미국자리공과 원추천인국은 포자가 형성되면서 clear zone이 22 mm와 19 mm로 나타났다. 자생식물인 까마중과 귀화식물인 미국자리공과 돼지풀에서 토양미생물인 Bacillus sphiaericus 2362와 Bacillus sphiaericus 2291, Bacillus thuringiensis var. subtilis와 Bacillus thuringiensis var. cereus에서 항균력이 나타났는데 돼지풀이 각 균주에서 항균활성이 가장 크게 나타났다. 특히 Bacillus thuringiensis var. subtilis에서 clear zone이 32 mm로 활성이 강하게 조사되었다. 일반적으로 추출액내의 가용성 고형물 함량이 높을수록 항균 활성이 크게 나타남을 알 수 있다. 자생식물인 까마중과 귀화식물인 미국자리공과 돼지풀의 추출액을 HPLC로 성분분석한 결과 까마중은 hydroquinone외 3종이, 미국자리공은 cinnamic acid외 5종이, 돼지풀은 benzoic acid외 7종이 분석되었다. Phenolic compound 각각에 대한 Aspergillus phoenicis KCTC 1228에 대한 항균력은 coumaric acid와 benzoic acid에서 조사되었다.

• The Plant Pathology Journal
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• 제15권5호
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• pp.308-310
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• 1999

A fungus associated with gray blight on tea plant (Camellia sinensis) was identifed as Pestalotiopsis theae based on the mycological characteristics. Mycelial growth on potato dextrose agar and size and shape of conidia of P. theae were similar to those of P. longiseta, but P. theae was different from P. longiseta in the color of three median cells and the number of apical appendages. Artificial inoculation of conidial suspension or mycelial mats on the wounded leaves and shoots of healthy plants induced the same disease, respectively. The Korean native variety was relatively. The Korean native variety was relatively more resistat to P. theae than a Japanese variety‘Yabukita’which has bee recently introduced and planted in large areas of southern parts of Korea. Here, we report the report the first record of gary blight caused by P. theae on tea plant in Korea.

• The Plant Pathology Journal
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• 제31권4호
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• pp.323-333
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• 2015

As sessile organisms, plants are exposed to persistently changing stresses and have to be able to interpret and respond to them. The stresses, drought, salinity, chemicals, cold and hot temperatures, and various pathogen attacks have interconnected effects on plants, resulting in the disruption of protein homeostasis. Maintenance of proteins in their functional native conformations and preventing aggregation of non-native proteins are important for cell survival under stress. Heat shock proteins (HSPs) functioning as molecular chaperones are the key components responsible for protein folding, assembly, translocation, and degradation under stress conditions and in many normal cellular processes. Plants respond to pathogen invasion using two different innate immune responses mediated by pattern recognition receptors (PRRs) or resistance (R) proteins. HSPs play an indispensable role as molecular chaperones in the quality control of plasma membrane-resident PRRs and intracellular R proteins against potential invaders. Here, we specifically discuss the functional involvement of cytosolic and endoplasmic reticulum (ER) HSPs/chaperones in plant immunity to obtain an integrated understanding of the immune responses in plant cells.

• 한국조경학회지
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• 제17권2호
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• pp.41-46
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• 1989

꼬리진달래는 상록활엽관목으로 내음성이 강하고 꽃이 아름다운 수종이어서 조경수목으로 개발할 가치가 매우 크다. 본 조사에서 얻은 결과를 요약하면 다음과 같다. 1. 꼬리진달래는 음지식물이며 대기습도가 높은 곳을 선호하며, 자생지토양은 산성이 강한 척박지이다. 2. 자생지의 방위는 대부분 북향에서 동북향이다. 3. 표고가 낮은 (230m 내외) 지역에서도 생육이 가능하다. 4. 자생지의 식생조사 결과는 소나무와 꼬리진달래가 우점종으로 나타났으며 이와 같은 군집 속에는 팥배나무와 원추리 아군집, 산딸기나무 아군집, 호랑버들 아군집이 나타났다. 5. 자생지의 식생율은 0∼80%였고 출현종수는 27종에서 50종까지 나타났다.