• Korean Journal of Plant Resources
• /
• v.17 no.1
• /
• pp.20-23
• /
• 2004

These studies were carried out to develop native Calanthe in Korea. Calanthe native to southern islands in korea has beautiful flowers with various color and sweet fragrance, and it has been reported to have very good ornamental value. Concerning the classified 57 lines had surveyed their characteristics during the three years, and confirmed which that had manifestation stably. And then, a line was developed and given a name of horticultural cultivar to ‘Saegdong’. The major characteristics of the selected line, ‘Saegdong’, were as follows. In its color of flower, sepal was reddish orange, petal was yellow and lip was yellow. ‘Saegdong’ had a bended-inner blooming type.

• Journal of Plant Biotechnology
• /
• v.47 no.4
• /
• pp.330-336
• /
• 2020

Ex-situ conservation of the ornamental and medicinal orchid, Coelogyne stricta, was performed by mass propagation using seed culture. Propagation stages were optimized using full- and half-strength solidified MS medium with different phytohormones. Maximum seed germination (88 ± 0.5% over 6 weeks of culture) was achieved on half-strength MS medium supplemented with 15% coconut water. Maximum shoot numbers were found on full-strength MS medium supplemented with 1 mg/L BAP, 2 mg/L Kinetin, and 10% coconut water, while the longest root was developed on full-strength MS medium with 1.5 mg/L IBA. A 2:1:1 combination of coco-peat, pine bark, and sphagnum moss was found to be a suitable potting mixture resulting in 80% seedling survivability. The cytotoxic activity of extracts of both wild plants and in vitro-developed protocorms was determined using an MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay on a cervical cancer cell line. The wild plant extract inhibited the growth of 41.99% of cells, showing that this extract has moderate cytotoxic activity toward cervical cancer cells.

• Journal of Plant Biotechnology
• /
• v.43 no.1
• /
• pp.132-137
• /
• 2016

The genus Cypripedium, known as lady's slipper orchid, has been considered to have a considerable marketability as a potted and garden plant with beautiful flowers; however, this species is becoming endangered and, in some places extinct, due to habitat destruction and illegal collections. As such, an optimized artificial propagation system is necessary for its conservation and horticultural cultivation. For the establishment of the in vitro proliferation of the endangered Cypripedium macranthos Sw., native to Korea, the effects of medium strength and sucrose concentrations on germination, protocorm formation and seedling growth were investigated through asymbiotic seed culture. The highest germination rate and protocorm formation rate were obtained with a 1/4 MS medium; higher MS medium strengths did not generate the favorable conditions required for germination and protocorm growth. The overall growth characteristics of roots and buds developed from protocorms were best in cultures of 1/4 MS medium. On this medium containing $10g{\cdot}L^{-1}$ sucrose, both the germination rate and protocorm formation rate increased significantly. The general growth properties of seedlings developed on the medium with $10g{\cdot}L^{-1}$ sucrose were best, showing the highest bud formation rate and root number. Our results demonstrate that the 1/4 MS medium, supplemented with $10g{\cdot}L^{-1}$ sucrose, could improve in vitro germination, and facilitate the growth of seedlings developed from the protocorms of C. macranthos Sw.

• Horticulture, Environment, and Biotechnology : HEB
• /
• v.59 no.6
• /
• pp.805-813
• /
• 2018

Vanda is an aerial tropical orchid native to Thailand and nitrogen (N) fertilizer is mainly used to promote its growth and quality. However, little is known about the characteristics of N absorption and assimilation in Vanda. The objective of this study was to determine the appropriate source of N for Vanda cultivation. In this experiment, shoots and roots of Vanda 'Ratchaburi Fuchs-Katsura' were sprayed weekly with 100 ml of $^{15}N$ tracer solution (1) 10 mM of $^{15}NO_3{^-}$, (2) 5 mM of $^{15}NO_3{^-}$ plus 5 mM of $NH_4{^+}$, (3) 5 mM of $NO_3{^-}$ plus 5 mM of $^{15}NH_4{^+}$ and (4) 10 mM of $^{15}NH_4{^+}$. The results indicated that plants fed with a combined N fertilizer gave the highest of $^{15}N$ use efficiency ( $^{15}NUE$) of about 21.8%, 30 days after the first feeding (DAF), compared with those fed sole sources of $^{15}NO_3{^-}$ (21.0%) and $^{15}NH_4{^+}$ (16.6%). However, a sole nitrate fertilizer or combination fertilizer did not significantly affect the total N and labelled N content. Alanine was a major amino acid found in leaves and roots at 7 DAF, whereas glutamine was mainly found in stems. At 30 DAF, tyrosine and alanine became major components in the leaves, and glutamine decreased in stems when plants were fed with a single $^{15}NH_4{^+}$ source.

• Korean Journal of Plant Taxonomy
• /
• v.49 no.2
• /
• pp.118-126
• /
• 2019

The taxonomic rank of the tiny-leaved terrestrial orchid Cephalanthera subaphylla Miyabe & $Kud{\hat{o}}$ has been somewhat controversial, as it has been treated as a species or as an infraspecific taxon, under C. erecta (Thunb.) Blume [C. erecta var. subaphylla (Miyabe & $Kud{\hat{o}}$) Ohwi and C. erecta f. subaphylla (Miyabe & $Kud{\hat{o}}$) M. Hiro]. Allozyme markers, traditionally employed for delimiting species boundaries, are used here to gain information for determining the taxonomic status of C. subaphylla. To do this, we sampled three populations of five taxa (a total of 15 populations) of Cephalanthera native to the Korean Peninsula [C. erecta, C. falcata (Thunb.) Blume, C. longibracteata Blume, C. longifolia (L.) Fritsch, and C. subaphylla]. Among 20 putative loci resolved, three were monomorphic (Dia-2, Pgi-1, and Tpi-1) across the five species. Apart from C. longibracteata, there was no allozyme variation within the remaining four species. Of the 51 alleles harbored by these 17 polymorphic loci, each of the 27 alleles at 14 loci was unique to a single species. Accordingly, we found low average values of Nei's genetic identities (I) between ten species pairs (from I = 0.250 for C. erecta versus C. longifolia to I = 0.603 for C. falcata vs. C. longibracteata), with C. subaphylla being genetically clearly differentiated from the other species (from I = 0.349 for C. subaphylla vs. C. longifolia to 0.400 for C. subaphylla vs. C. falcata). These results clearly indicate that C. subaphylla is not genetically related to any of the other taxa of Cephalanthera that are native to the Korean Peninsula, including C. erecta. In a principal coordinate analysis (PCoA), C. subaphylla was positioned distant not only from C. falcata, C. longibracteata, and C. longifolia, but also from C. erecta. Finally, K = 5 was the best clustering scheme using a Bayesian approach, with five clusters precisely corresponding to the five taxa. Thus, our allozyme results strongly suggest that C. subaphylla merits the rank of species.

• Horticultural Science & Technology
• /
• v.16 no.3
• /
• pp.361-363
• /
• 1998

Cymbidium goeringii native to Korea and other orchid plants were pot-grown from spring to autumn under the greenhouse conditions, and were subjected to artificial pollination to elucidate the compatibility by revealing viable seed formation. A notable compatibility was found when Cym. goeringii was selfed and was crossed with either Cym. ensifolium, Cym. kanran, Cym. sinense, Cym. sinense for. albo-jucundissimum, Cym. 'Crystal Cherry Angel', or Cym. 'Anmitsu Hime'. Cym. goeringii, however, did not show such compatibility when crossed with either Cym. faberi, Cym. aloifolium, Dendrobium chrysotoxum, or Phalaenopsis spp. RAPD analysis indicated that taxa relationship between Cym. goeringii and either Cym. faberi or Cym. aloifolium (respective chromosome number, 2n=40) was distant, showing no compatibility, and even more distant in the case of cross-pollination between Cym. goeringii and either Dendrobium chrysotoxum or Phalaenopsis spp. having different chromosome number from all Cymbidium species.

• Horticultural Science & Technology
• /
• v.17 no.3
• /
• pp.346-351
• /
• 1999

According to their flower color and the shape of petal, sepal, and lip, Calanthe species native to Cheju Island are named as follows; 'Arang', 'Cheongsu', 'Sara', 'Aeyeol', 'Darigot', 'Saebaengi', 'Andeok', 'Setbyeol', 'Kyorae', 'Jasujeong', 'Baekrok', 'Seokwang', 'Deokcheon', 'Seonheul', 'Saechimi', 'Jokduri', 'Baedal', 'Noksu', 'Noeul', and 'Dansim for 20 cultivars of Calanthe discolor; 'Hwanghee', 'Waheul', 'Illchool', 'Hwangryong', 'Dabo', 'Hwangjinee', 'Daeha', 'Chwikwang', 'Seokyang', 'Eunpa', 'Chwiseon', 'Sinbaram', 'Ara', 'Dano', 'Pakae', and 'Sojeob' for 16 cultivars of Calanthe sieboldii; 'Dongkwang', 'Myeongdeok', 'Wangdolseon', 'Myeongryeok', 'Kwibuin', 'Semi', 'Cheonwangsa', 'Mandeok', 'Seolmundae', 'Kyeongok', 'Darangsi', 'Byeolbang', 'Honghak', 'Dongbaeksan', 'Chejudo', 'Samda', 'Kwandeok', 'Youngjusan', 'Tamra', 'Koyeongdi', 'Yongduam', and 'Jeolbuam' for 22 cultivars of Calanthe bicolor.

• Mycobiology
• /
• v.45 no.4
• /
• pp.312-317
• /
• 2017

Orchidaceous plants have symbiotic relationships with endophytic fungi, including mycorrhizal fungi, which play important roles in the seed germination and growth of the host plants. In this study, endophytic fungal communities isolated from the roots of Cephalanthera longibracteata collected from three different sites in Korea were analyzed, and it was determined whether fungal communities were preferentially correlated with the sites. The fungal isolates were identified by sequence analysis of the internal transcribed spacer regions of rDNA. In total, 30 species of endophytic fungi, including two species of mycorrhizal fungi belonging to the genus Tulasnella, were identified. Leptodontidium orchidicola showed the highest frequency and was isolated from all root samples. Species diversity and richness were not significantly different among sites. However, the community structure of the endophytic fungi significantly differed among sites, suggesting that the site characteristics affected the community composition of the endophytic fungi colonizing the roots of C. longibracteata. Our findings will aid in developing methods involving the use of symbiotic fungi for orchid conservation and restoration in native habitats.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.7 no.1
• /
• pp.40-49
• /
• 2004

This work carried out to define the characteristic of Cymbidium kanran habitat at Mt. Halla in Jejudo, Korea from the ecological point of view including geological and topographical features, air and soil temperature, relative humidity, fluctuations of light intensity, habitat vegetation, distribution altitude, area limit, and etc. And another goal of this study consider the conservation counterplan of the cymbidium habitat. Natural distribution areas of the cymbidium were observed more abundantly on the well drained south and east-facing slopes. Soil acidity was ranged from pH 4.1 to 5.3, and electric conductivity was ranged from 176.4 to 299.9 us/cm (average 215.3 us/cm). Base-saturation percentage of the habitat soil was below 50%, bulk density 0.42g/$cm^2$, particle density 2.05g/$cm^2$, humus content 26%, total nitrogen 0.82%, available phosphate 4.2 mg/kg, exchangeable potassium 0.63 Cmol/kg, calcium 0.44 Cmol/kg and magnesium 0.67 Cmol/kg. Annual mean air temperature was $15.4^{\circ}C$, however, air temperature was ranged $11.7{\sim}18.2^{\circ}C$ in spring, $21.2{\sim}23.8^{\circ}C$ in summer, $12.8{\sim}22.0^{\circ}C$ in fall and $5.5{\sim}7.8^{\circ}C$ in winter season. Annual mean soil temperature at depth of 10cm was $13.2^{\circ}C$ And minimum value was recorded $4.7^{\circ}C$ on January, and maximum value $22.5^{\circ}C$ on August. Relative humidity was ranged 90.8~94.7% in summer, 80.8~91.5% in fall and 77.6~84.2% in winter season. Minimum value was 56.5% on December, and maximum value was 100% on July and August. Light intensities were ranged from 400 to 1,800 lux at the greater part of Cymbidium kanran sites in Jejudo. Summer regarded as an lower light intensities was recorded to be range of 500~600 lux; however, autumn and winter were shown higher light regimes ranged from 3,500 to 3,800 lux. Therefore, one must be suprised that the cymbidium grow at the light condition of 6 lux (minimum) or 10,000 lux (maximum). Tree species keeping higher frequency rate and density were Eurya japonica, Camellia japonica, Castanopsis cuspidata, Carpinus laxiflora and Pinus densiflora. Number of trees growing in a 5${\times}$5m quardrat was 35 as an average, and proportion of evergreen versus deciduous was 5:1. Distribution altitude of the orchid habitat was ranged from 120m (low) to 840m (high) from sea level on the south facing slope of Mt. Halla, and was ranged eastern borderline of Gujwaup, Bukjejugun to western boundaries of Jungmundong, Seogwipo city. For the stable conservation of Cymbidium kanran habitat, sunlight regimes must be increased more by means of cutting trees or twigs in the site.

• Horticultural Science & Technology
• /
• v.17 no.2
• /
• pp.141-143
• /
• 1999

Genetic relationship of Calanthe discolor, C. sieboldii, and C. bicolor for elucidating the classification was investigated. Electrophoretic zymograms for either peroxidase or esterase isozymes indicated that bands of C. bicolor appear in the zone where those of C. discolor and C. sieboldii are located. Genetic relationship among the three Calanthe species using RAPD analysis showed that C. discolor and C. sieboldii are more distant each other than C. bicolor, demonstrating the genetic position of C. bicolor between the other two. It was assumed that C. bicolor is a natural hybrid between C. discolor and C. sieboldii.