• 한국물환경학회지
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• 제32권5호
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• pp.419-432
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• 2016

이 연구의 목적은 응천에서 채집한 담수 붕어(Carassius auratus)의 산화적 스트레스와 조직병리학적 손상을 분석하여 하수처리장 방류수의 영향을 평가하는 것이다. 방류수 혼합 지점과 하류 지점에서 채집한 붕어의 아가미, 간, 콩팥의 과산화수소 분해효소(catalase)의 활동도는 상류 지점과 비교하여 더 높은 것으로 나타났다. 또한, 혼합 지점의 아가미와 간의 글루타치온 S-전이효소(glutathione-S-transferase)의 활동도는 상류 지점과 비교하여 유의하게 높았으며(p < 0.05), 상류 지점보다 유의하게 더 증가된 간의 지질 과산화(lipid peroxidation)가 혼합 지점에서 관찰되었다. 붕어의 조직병리학적 손상의 정도는 간 > 콩팥 > 아가미순으로 나타났다. 이 결과는 간이 방류수의 영향을 가장 크게 받는다는 것을 의미한다. 붕어의 조직 손상도(degree of tissue changes)는 혼합 지점에서 채집한 시료(30.98 ± 5.40)에서 가장 컸으며, 그 다음은 하류 지점 시료(19.28 ± 4.31)이고 가장 낮은 것은 상류 지점 시료(4.83 ± 2.67)로 나타났다. 이러한 결과는 혼합 지점에서 채집한 붕어가 방류수에 가장 큰 영향을 받았다는 것을 의미하며, 산화적 스트레스와 조직병리학적 지표들이 방류수로 오염된 하천을 모니터링하는데 유용하다는 것을 나타낸다.

• 한국수산과학회지
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• 제45권3호
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• pp.238-245
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• 2012

In this study, the molecular structures of tet(M) and tet(G) carried by tetracycline (Tc) resistant bacteria in intestinal microflora from the imported ornamental fish were characterized and compared with each other depend on the imported countries. Of the total isolates, approximately 8.9% of the Ent-lac+(lactose fermentative bacteria on coliform media) Tc resistant isolates in fish from three different countries, Singapore, Taiwan and Brazil, were appeared to contain tet(M). Three representative isolates of different countries, Aeromonas spp. JSM-1 (Singapore), JTM-1 (Taiwan) and JBM-1 (Brazil), were isolated and analyzed the molecular structures of tet(M) gene. Interestingly, partial sequence of tet(M) genes (1099 bp) in JBM-1 (Brazil) showed 99.5% homology with the tet(M) found in the Vibrio spp. RV16 isolate, obtained from marine fish in Korea and known to carry Tn1545 parent type of tet(M). In contrast, tet(M) gene in JSM-1 and JTM-1 showed mosaic structure of Tn1545 and Tn916, and 100% homology with each other. It may suggest the presence of various characteristics in terms of tet(M) gene structure. The determined sequence of the tet(G) from Aeromonas spp. JSG-1 and JBG-1 isolated from Singapore and Indonesia ornamental fish respectively showed similar nucleotide sequence homology but revealed a few nucleotide changes in comparison with the sequence of the prototype tet(G) gene (S52437 in GenBank).

• 한국수산과학회지
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• 제44권3호
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• pp.225-231
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• 2011

Prolactin (PRL) plays an important role in freshwater (FW) osmoregulation by preventing the loss of ions and the uptake of water in fish. Growth hormone (GH) promotes acclimation to seawater (SW) in several teleosts. We acclimated rainbow trout Oncorhynchus mykiss weighting $68.2{\pm}16.6$, $138.3{\pm}24$, and $287.5{\pm}42.1$ g in separate experiments to SW under slow-acclimation (SSW) or acute-acclimation (ASW) conditions, and then examined the PRL and GH mRNA levels using the real-time quantitative polymerase chain reaction. The PRL mRNA levels in all three experimental groups decreased significantly with both the SSW and ASW treatments, as compared to a control group kept in FW for 30 days. The GH mRNA levels increased with ASW in the largest fish, whereas the levels in the other groups did not change significantly. The mortality rate of the largest fish was lower than for the other groups, whereas the growth rate among the three experimental groups did not differ significantly. The growth rate of the ASW group was highest for the smallest fish. These results suggest that SW acclimation is associated with the gene expression levels of PRL and GH in relatively large rainbow trout. In addition, the fish mortality and growth rate on FW-SW transfer seem to be related to body weight, and the SW acclimation method may be applied to the hatcheries industry.

• 한국수산과학회지
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• 제51권3호
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• pp.248-253
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• 2018

Freshwater farms are primarily located adjacent to rivers and lakes, facilitating the introduction and spread of pathogens into natural systems. Therefore, it is necessary to continuously monitor natural aquatic organisms, the breeding environment, and infection rates by pathogenic organisms. Fish and crustaceans were sampled 4 times in the Geum River estuary in 2016. The samples were analyzed for the presence of pathogens for reportable communicable diseases, including KHVD (koi herpesvirus disease), SVC (spring viraemia of carp), EUS (epizootic ulcerative syndrome) and WSD (white spot disease); parasite abundance was also examined. The dominant fish species were deep body bitterling Acanthorhodes macropterus (21.4%), followed by skygager Erythroculter erythropterus (12.7%). For crustaceans, Palaemon paucidens and Chinese mitten crab Eriocheir sinensis were dominant. Sixty fish and 36 crustacean species were examined for reportable communicable diseases. When using a specific primer set for each disease, PCR analysis did not detect any reportable communicable diseases in the samples. Some instances of Dactylogyrus, copepods, nematodes and metacercaria were detected. However, the PCR results indicated that the metacercaria were not Clonorchis sinensis.

• 한국물환경학회지
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• 제29권6호
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• pp.746-753
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• 2013

The aim of this study was to evaluate artificial fish shelter, which was known to increase prey survival and expand habitat space to improve species diversity and fish communities in a freshwater ecosystem. The experiment was performed at an outdoor test-bed for three months from 2011 by comparing the responses to adjustments in the volume of the artificial patch (juvenile fish shelter, JFS) in the control and experimental groups. Analysis of the environmental conditions over two periods (Period1 ~ 2) showed minor differences in the physichemical characteristics of water quality, phytoplankton, and zooplankton biomass, thus, allowing comparative analysis of feeding ecology. However, high water temperature conditions in Period1 ($25.6{\pm}2.0^{\circ}C$), affected the predation activity of the piscivorous fishes, Coreoperca herzi (C. herzi, size $89{\pm}4mm$). Survival rates of the prey fishes, Rhynchocypris oxycephalus (R. oxycephalus, size $29{\pm}1mm$), improved as the patch volume increased and were higher than those of the control group by 35.9 ~ 46.7%. Analysis showed that JFS reduced the chances of predator-prey encounter, and thereby minimized prey vulnerability.

• Ecology and Resilient Infrastructure
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• 제10권1호
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• pp.11-19
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• 2023

전세계적으로 외래생물의 침입이 생물다양성을 위협하는 것으로 보고되며, 국내외로 외래생물의 유입에 대한 관심이 증가하고 있다. 우리나라 환경부에서는 유입주의 생물 지정을 통해 국내에 유입되었을 때 생태계 피해가 유발될 수 있는 잠재적 외래생물을 관리하고 있다. 본 연구에서는 유입주의 생물로 지정된 포식성 어류인 북방민물꼬치고기 (Esox lucius)와 호주민물대구 (Maccullochella peelii)의 잠재 서식처를 전국 단위로 예측하였다. 서식처 적합도 평가를 위해 EHSM (Ecological Habitat Suitability Model)을 사용하였고, 수온 자료를 입력하여 생리적 서식처 적합도 지수 (Physiological Habitat Suitability; PHS)를 산출하였다. 예측 결과, 두 어류의 PHS는 고온 또는 저온 스트레스의 영향을 크게 받았으며, 이로 인해 서식처 적합도의 지역적 편향이 나타났다. 북방민물꼬치고기는 위도가 높은 한강과 금강 유역을 선호할 것으로 예측되었고, 호주민물대구는 대도시 지역을 선호하는 것으로 나타났다. 이러한 결과를 통해 외래어류의 침입 양상이 온도에 대한 선호도의 차이로 인해 상이할 것으로 예상할 수 있다. 향후, 모델의 예측력 향상을 위해 후속 연구가 필요하고, 지속가능한 대책 수립을 위해 기후변화 시나리오를 적용한 미래 예측 연구가 필요하다.

• Animal Systematics, Evolution and Diversity
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• 제33권2호
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• pp.136-139
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• 2017

The genus Iksookimia (Actinopterygii: Cypriniformes: Cobitidae) is a bottom-dwelling freshwater loaches, which are well-known as their endemism and high geographic variation. However, population genetic relationships among Iksookimia spp. have remained unclear due to a shortage of genetic markers that can be applied generally in the genus. Here, we developed high-resolving microsatellite markers using I. koreensis and I. longicorpa as representatives of Iksookimia species because of their wide distribution range and phylogenetic position. Ten of polymorphic microsatellite loci were isolated from Iksookimia koreensis and were successfully cross-amplified in I. longicorpa. The mean number of observed alleles per locus was about 10.4 (range, 2-17) for I. koreensis and about 13.2 (range, 2-24) for I. longicorpa. The loci, IK03 and IK08, deviated from the Hardy-Weinberg equilibrium in I. koreensis, after applying the Bonferroni correction. The microsatellite markers obtained in the present study will be useful to evaluate population genetic structure and to establish conservation strategies for I. koreensis and related Iksookimia species.

• 한국어류학회지
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• 제30권2호
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• pp.119-124
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• 2018

낙동강 수계의 임하호에 서식하는 담수어류의 다양성과 종 목록을 고찰하고자 1992년부터 2016년까지의 문헌을 분석하였다. 그 결과, 임하호 내에서 출현한 전체 어류는 총 4목 11과 36속 46종 23,269개체가 보고되었다. 잉어과가 27종(58.7%), 미꾸리과 5종 (10.9%), 망둑어과 3종 (6.5%), 메기과, 꺽지과, 검정우럭과가 각각 2종(4.3%)씩 보고되었다. 우점종은 9,333개체 (40.1%)가 출현한 강준치였고, 아우점종은 2,281개체(9.8%)가 출현한 끄리로 나타났다. 우리나라 고유종은 17종으로 출현어종 46종의 37.0%를 차지하였고, 환경부 지정 멸종위기종은 3종, 국내이입종은 5종, 외래종은 3종이 보고되었다.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제2권1호
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• pp.42-52
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• 2021

In order to secure basic data on biodiversity for wetland conservation and management used the data from Wetland Protected Area surveys conducted in South Korea (2015-2019) to analyze the distribution of fish from a total of 15 orders, 45 families, 134 species, and 12,972 individuals. The predominant species identified were Zacco platypus (Temminck and Schlegel) (19.47%) and Zacco koreanus (Kim, Oh and Hosoya) (8.16%). Of all emergent species, 52.9% (n=71 species) were freshwater species, 26.9% (n=36) were brackish species, 3.0% (n=4) were migratory species, 27% (n=36) were marine species, and 9.0% (n=12) were riffle benthic species. Overall, 5.2% (n=7 species) were endangered species, 3.0% (n=4) were exotic species, and 23.1% (n=31) were Korean endemic species. The eight identified Wetland Protected Areas (WPA) were classified based on their habitat characteristics and on the analysis of their emergent fish communities, as estuarine (n=2), coastal dune (n=1), marsh (n=2), stream (n=2), and stream-marsh (n=1) types. The environmental factors revealed to have the greatest influence on the species diversity of emergent fish were maintenance and repair, installation of reservoirs, and construction of artificial wetlands around them. The present study offers basic information on the diversity of fish species in different Wetland Protected Area types that can be used to inform conservation and management decisions for WPA.

• Parasites, Hosts and Diseases
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• 제59권1호
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• pp.97-101
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• 2021

The infection status with Clonorchis sinensis metacercariae (CsMc) was examined in freshwater fishes from Yongjeon-cheon (a branch of Nakdong-gang) located in Cheongsong-gun, Gyeongsangbuk-do, the Republic of Korea (Korea). A total of 750 fishes in 19 species were examined by the artificial digestion method for 2 years (2019 and 2020). CsMc were detected in 378 (51.4%) out of 735 fishes in 14 species (73.7%), and the infection intensity was 666 per fish infected. In 2019, CsMc were found in 172 (68.0%) out of 253 fishes in 10 species, and the infection intensity was 565 per fish infected. In 2020, CsMc were detected in 206 (62.2%) out of 331 fishes in 10 species, and the infection intensity was 751 per fish infected. The other zoonotic trematode, ie. Metagonimus spp., Centrocestus armatus, Echinostoma spp. and Clinostomum complanatum, metacercariae were also detected in fishes from the survey streams, but their endemicities were relatively low. Conclusively, it was first confirmed that CsMc are highly endemic in fishes from Yongjeon-cheon in Cheongsong-gun, Gyeongsangbuk-do, Korea.