Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Characterization of tet(M) and tet(G) Genes among Tetracycline-resistant Aeromonas spp. Isolated from Imported Ornamental Fishes

수입산 관상어로부터 분리된 tetracycline 내성 Aeromonas spp.에 tet(M) 및 tet(G) 유전자의 특성 분석

  • Park, Shin-Hoo (Future Aquaculture Research Center, National Fisheries Research & Development Institute) ;
  • Jun, Lyu-Jin (School of Marine Biomedical Science, College of Ocean Science, Jeju National University) ;
  • Cho, Ki-Taek (Gyeonggi Province Freshwater Fisheries Research Institute) ;
  • Jin, Ji-Woong (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Jeong, Hyun-Do (Department of Aquatic Life Medicine, Pukyong National University)
  • 박신후 (국립수산과학원 미래양식연구센터) ;
  • 전려진 (제주대학교 해양생명과학과) ;
  • 조기택 (경기도 민물고기연구소) ;
  • 진지웅 (부경대학교 수산생명의학과) ;
  • 정현도 (부경대학교 수산생명의학과)
  • Received : 2012.03.29
  • Accepted : 2012.05.01
  • Published : 2012.06.30
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Abstract

In this study, the molecular structures of tet(M) and tet(G) carried by tetracycline (Tc) resistant bacteria in intestinal microflora from the imported ornamental fish were characterized and compared with each other depend on the imported countries. Of the total isolates, approximately 8.9% of the Ent-lac+(lactose fermentative bacteria on coliform media) Tc resistant isolates in fish from three different countries, Singapore, Taiwan and Brazil, were appeared to contain tet(M). Three representative isolates of different countries, Aeromonas spp. JSM-1 (Singapore), JTM-1 (Taiwan) and JBM-1 (Brazil), were isolated and analyzed the molecular structures of tet(M) gene. Interestingly, partial sequence of tet(M) genes (1099 bp) in JBM-1 (Brazil) showed 99.5% homology with the tet(M) found in the Vibrio spp. RV16 isolate, obtained from marine fish in Korea and known to carry Tn1545 parent type of tet(M). In contrast, tet(M) gene in JSM-1 and JTM-1 showed mosaic structure of Tn1545 and Tn916, and 100% homology with each other. It may suggest the presence of various characteristics in terms of tet(M) gene structure. The determined sequence of the tet(G) from Aeromonas spp. JSG-1 and JBG-1 isolated from Singapore and Indonesia ornamental fish respectively showed similar nucleotide sequence homology but revealed a few nucleotide changes in comparison with the sequence of the prototype tet(G) gene (S52437 in GenBank).

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References

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