Browse > Article
http://dx.doi.org/10.5657/KFAS.2012.0238

Characterization of tet(M) and tet(G) Genes among Tetracycline-resistant Aeromonas spp. Isolated from Imported Ornamental Fishes  

Park, Shin-Hoo (Future Aquaculture Research Center, National Fisheries Research & Development Institute)
Jun, Lyu-Jin (School of Marine Biomedical Science, College of Ocean Science, Jeju National University)
Cho, Ki-Taek (Gyeonggi Province Freshwater Fisheries Research Institute)
Jin, Ji-Woong (Department of Aquatic Life Medicine, Pukyong National University)
Jeong, Hyun-Do (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.45, no.3, 2012 , pp. 238-245 More about this Journal
Abstract
In this study, the molecular structures of tet(M) and tet(G) carried by tetracycline (Tc) resistant bacteria in intestinal microflora from the imported ornamental fish were characterized and compared with each other depend on the imported countries. Of the total isolates, approximately 8.9% of the Ent-lac+(lactose fermentative bacteria on coliform media) Tc resistant isolates in fish from three different countries, Singapore, Taiwan and Brazil, were appeared to contain tet(M). Three representative isolates of different countries, Aeromonas spp. JSM-1 (Singapore), JTM-1 (Taiwan) and JBM-1 (Brazil), were isolated and analyzed the molecular structures of tet(M) gene. Interestingly, partial sequence of tet(M) genes (1099 bp) in JBM-1 (Brazil) showed 99.5% homology with the tet(M) found in the Vibrio spp. RV16 isolate, obtained from marine fish in Korea and known to carry Tn1545 parent type of tet(M). In contrast, tet(M) gene in JSM-1 and JTM-1 showed mosaic structure of Tn1545 and Tn916, and 100% homology with each other. It may suggest the presence of various characteristics in terms of tet(M) gene structure. The determined sequence of the tet(G) from Aeromonas spp. JSG-1 and JBG-1 isolated from Singapore and Indonesia ornamental fish respectively showed similar nucleotide sequence homology but revealed a few nucleotide changes in comparison with the sequence of the prototype tet(G) gene (S52437 in GenBank).
Keywords
Tetracycline; Ornamental fish; Tet(M); Tet(G);
Citations & Related Records
연도 인용수 순위
  • Reference
1 Zhao J and Aoki T. 1992. Nucleotide sequence analysis of the class G tetracycline resistance determinant from Vibrio anguillarum. Microbiol Immunol 36, 1051-1060.   DOI
2 Nawaz M, Khan AA, Khan S, Sung K and Steele R. 2008. Isolation and characterization of tetracycline resistant Citrobacter spp. from catfish. Food Microbiol 25, 85-91.   DOI   ScienceOn
3 Oggioni MR, Dowson CG, Smith JM, Provvedi R and Pozzi G. 1996. The tetracycline Resistance gene tet(M) exhibits mosaic structure. Plasmid 35, 156-163.   DOI
4 Provvedi R, Mangannelli R and Pozzi G. 1996. Characterization of conjugative transposons Tn5251 of Streptococcus pneumoniae. FERMS Microbiol Lett 135, 231-236.   DOI
5 Rice LB. 1998. Tn916 family conjugative transposons and dissemination of antimicrobial resistance determinants. Antimicrob Agents Chemother 42, 1871-1877.
6 Roberts MC. 1996. Tetracycline resistance determinants: mechanisms of action, regulation of expression, genetic mobility, and distribution. FEMS Microbilor Review 19, 1-24.   DOI
7 Roberts MC. 2005. Update on acquired tetracycline resistance genes. FEMS Microbiol Lett 245, 195-203.   DOI
8 Roberts MC. 2011. Environmental macrolide-lincosamidestreptogramin and tetracycline resistant bacteria. Front Microbiol 2, 1-8.
9 Schmidt AS, Bruun MS, Dalsgaard I and Larsen JL. 2001. Incidence, distribution, and spread of tetracycline resistance determinants and integron associated antibiotic resistance genes among motile aeromonads from a fish farming environment. Appl Environ Microbiol 67, 5675-5682.   DOI   ScienceOn
10 Srinivasan V, Nam HM, Sawant AA, Headrick SI, Nguyen LT and Oliver SP. 2008. Distribution of tetracycline and streptomycin resistance genes and class 1 integrons in Enterobacteriacae isolated from dairy and nondairy farm soils. Microb Ecol 55, 184-193.   DOI
11 Trust TJ and Whitby JL. 1976. Antibiotic resistance of bacteria in water containing ornamental fishes. Antimicrob Agents Chemother 10, 598-603.   DOI
12 Chopra I and Roberts MC. 2001. Tetracycline Antibiotics: Mode of Action, Application, Molecular Biology, and Epidemiology of Bacterial Resistance. Microbiol Mol Biol Rev 65, 232-260.   DOI   ScienceOn
13 Clewell DB, Flannagan SE and Jaworski DD. 1995. Unconstrained bacterial promiscuity: The Tn916-Tn1545 family of conjugative transposons. Trends Microbiol 3, 229-236.   DOI
14 Depaola A and Roberts MC. 1995. Class D and E tetracycline resistance determinants in gram-negative bacteria from catfish ponds. Mol Cell Proves 9, 311-313.   DOI   ScienceOn
15 Furushita M, Shiba T, Maeda T, Yahata M, Kaneoka A, Takahashi Y, Torii K, Hasegawa T and Ohta M. 2003. Similarity of Tetracycline resistance genes isolated from fish farm bacteria to those from clinical isolates. Appl Environ Microbiol 69, 5336-5342.   DOI   ScienceOn
16 Gascoyne-Binizi DM and Hawkey PM. 1993. Nucleotide sequence of tet(M) genes from the American and Dutch type tetracycline resistance plasmid of Neisseria gonorrhoeae. J Antimicrob Chemother 32, 667-676.   DOI
17 Levy SB, McMurry LM, Barbosa TM, Burdett V, Courvalin P, Hillen W, Roverts MC, Rood JL and Tayor DE. 1999. Nomenclature for new tetracycline resistance determinants. Antimicrob Agents Chemother 43, 1523-1524.
18 Jun LJ, Jeong JB, Huh MD, Chung JK, Choi DL, Lee CH and Jeong HD. 2004. Detection of tetracycline-resistance determinants by multiplex polymerase chain reaction in Edwardsiella tarda isolated from fish farms in Korea. Aquaculture 240, 89-100.   DOI
19 Kim YH, Jun LJ, Park SH, Yoon SH, Chung JK, Kim JC and Jeong HD. 2007. Prevalence of tet(B) and tet(M) genes among tetracycline-resistant Vibrio spp. in the aquatic environments of Korea. Dis Aguat Org 75, 209-216.   DOI
20 Kim SR, Nonaka L and Suzuki S. 2004. Occurrence of tetracycline resistance genes tet(M) and tet(S) in bacteria from marine aquatic sites. FEMS Microbiol Lett 237, 147-156.   DOI
21 Brenner KP, Rankin CC, Roybal YR, Stelma GN, Scarpino PV and Dufour AP. 1993. New medium for the simultaneous detection of total coliforms and Escherichia coli in water. Appl Environ Microbiol 59, 3534-3544.
22 Aminov RI, Chee-Sanford CC, Garrigues N, Teferedegne B, Krapac IJ, White BA and Mackie RI. 2002. Development, Validation, and Application of PCR Primers for Detection of Tetracycline Efflux Genes of Gram-Negative Bacteria. Appl Environ Microbiol 68, 1786-1793.   DOI
23 Bertram J, Stratz M and Durre P. 1991. Natural transfer of conjugative transposon Tn916 between gram-positive and gram-negative bacteria. J Bacteriol 173, 443-448.   DOI