• Nutrition Research and Practice
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• 제8권5호
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• pp.539-543
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• 2014

BACKGROUND/OBJECTIVES: Soy isoflavones are structurally similar to estrogen and bind to estrogen receptors, suggesting that they exhibit estrogenic activities; therefore, they are referred to as phytoestrogens. Fermentation may affect the bioavailability of isoflavones altering soy isoflavone glycosides in the form of aglycones. Thus, this study investigated the effects of fermented soybeans by Rhizopus oligosporus on bone metabolism in both young rats as a pilot test and in ovariectomized (ovx) old rats as a model of menopause. MATERIALS/METHODS: In the pilot test, a total of 24 seven-week-old female Sprague-Dawley (SD) rats were fed one of three diets for a period of four weeks: casein, unfermented soybean product, or fermented soybean product by R. oligosporus. In the ovx rat model, 20-week-old SD rats weighing 260-290 g underwent either sham-operation (n = 10) or bilateral ovariectomy (n = 30) and were then fed the AIN-93M diet for one week. Thereafter, rats were fed sham-casein, ovx-casein, ovx-soybean, or ovx-fermented soybean diet for five weeks. After decapitation, femoral bones were isolated and preserved in 9% formalin for assessment of bone mineral density (BMD), bone mineral content (BMC), and bone-breaking strength (BBS). RESULTS: Ovx rats showed significantly increased weight gain and decreased uterine wet weight. Of particular interest, ovx rats fed fermented soybeans showed increased uterine wet weights compared to control rats. Fermented soybean diet caused a significant increase in plasma 17-${\beta}$ estradiol concentrations in young rats, and 17-${\beta}$ estradiol levels were enhanced in ovx rats to match those of sham-operated ones. Significantly lower femoral BMD and BMC were observed in ovx rats compared to sham-operated controls, whereas bone areas did not differ statistically among the groups. In addition, BBS tended to be increased in ovx rats fed soybeans and fermented soybeans. CONCLUSIONS: Supplementation of fermented soybeans could have preventive and therapeutic effects against osteoporosis in postmenopausal women.

• Journal of Animal Science and Technology
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• 제58권7호
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• pp.26.1-26.11
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• 2016

The use of probiotics for human and animal health is continuously increasing. The probiotics used in humans commonly come from dairy foods, whereas the sources of probiotics used in animals are often the animals' own digestive tracts. Increasingly, probiotics from sources other than milk products are being selected for use in people who are lactose intolerant. These sources are non-dairy fermented foods and beverages, non-dairy and non-fermented foods such as fresh fruits and vegetables, feces of breast-fed infants and human breast milk. The probiotics that are used in both humans and animals are selected in stages; after the initial isolation of the appropriate culture medium, the probiotics must meet important qualifications, including being non-pathogenic acid and bile-tolerant strains that possess the ability to act against pathogens in the gastrointestinal tract and the safety-enhancing property of not being able to transfer any antibiotic resistance genes to other bacteria. The final stages of selection involve the accurate identification of the probiotic species.

• 한국식품위생안전성학회지
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• 제30권4호
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• pp.359-365
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• 2015

본 연구는 효소식품과 효소표방식품 (기타가공식품, 음료베이스, 기타발효음료, 액상차) 98건에 대한 ${\alpha}$-아밀라아제, ${\beta}$-아밀라아제 활성과 당 함량을 조사하였다. 효소식품과 기타가공품의 ${\alpha}$-아밀라아제 활성은 각각 4.9~53,854.6 U/g, 2.9~1,182.7 U/g으로 같은 유형간에 큰 차이가 있었다. 발효식품의 ${\alpha}$-아밀라아제 활성은 각각 0.1~1.7 U/g이었다. 효소식품, 기타가공품 그리고 발효식품의 ${\beta}$-아밀라아제 평균 활성은 각각 126.0 U/g, 5.6 U/g, 10.5 U/g으로 효소표방식품은 효소식품보다 훨씬 낮은 활성을 나타냈다. 평균 당 함량은 효소식품 22.4 g/100 g, 기타가공품 14.8 g/100 g, 음료베이스 46.9 g/100 g, 기타발효음료류 41.1 g/100 g, 액상차 39.5 g/100 g으로 발효식품에서 높은 당 함량을 나타냈다. ${\alpha}$-아밀라아제 활성과 유당 함량은 효소식품에서 통계적으로 강한 상관관계(r = 0.644)를 나타냈고 기타가공식품에서는 매우 강한 상관관계(r = 0.903)를 나타냈다. ${\beta}$-아밀라아제 활성과 유당 함량은 효소식품에서 통계적으로 강한 상관관계(r = 0.648)를 나타냈고 기타가공식품에서는 강한 상관관계(r = 0.757)를 나타냈다. 효소식품과 기타가공품에서 ${\alpha}$-아밀라아제 활성과 ${\beta}$-아밀라아제 활성 사이에는 매우 강한 상관관계(r = 0.869, r = 0.760)를 나타냈다. 즉, ${\alpha}$-아밀라아제 활성과 ${\beta}$-아밀라아제 활성 사이에 비례관계가 성립함을 알 수 있었다.

• 동아시아식생활학회지
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• 제7권1호
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• pp.41-46
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• 1997

Diet is generally accepted as one of the important factors in human cancer development. Ethyl carbamate has been associated with cancer for several decades and mainly found in the fermented beverages and foodstuff. The relationship between ethyl carbamate and the human health cannot be ignored especially in the areas where fermented foods consists of regular food consumption. To investigate the ethyl carbamate exposure level in Korean population, commercial fermented food samples were collected form local markets I Seoul area and home-made varieties were collected throughout the country. Following partial purification the concentration of ethyl carbamete was determined by GC/ MS. The concentration of ethyl carbamate ranged to 70 ppb in soysauce, to 10 ppb in soybean paste, and to 5 ppb in vinegars. Korean traditional alcoholic beverages showed small amount of ethyl carbamate. The estimated daily exposure of Korean population was 0~1900ng/day. It would be prudent to put efforts to minimize the formation of ethyl carbamate since the risk from the higher range exposure cannot be ignored.

• 한국식생활문화학회지
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• 제4권4호
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• pp.375-382
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• 1989

About 760 papers and patents of the traditional fermented food in Korea were collected which were published during 1917-1988, and then the papers were classified, outlined and reviewed. Study of traditional fermented food was about 10% of total study on Korean Foods, and that of alcoholic beverage was 50%, soy sauce and related product was 30% and kimchi was less than 20% of total study of traditional fermented foods. Though considerable amount of studies were accomplished before the World War II, but a few study was accomplished during the Korean war. The research activity is, hitherto, increasing gradually, but the systematic studies were rarely done. The studies to be investigated were processing quality of raw materials, classification, standardization, sensory quality of the product and so on.

• Journal of Microbiology and Biotechnology
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• 제12권4호
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• pp.649-656
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• 2002

Fibrinolytic microorganisms were screened from 42 samples of Korean fermented food (7 kinds of Chungook-jang, 14 kinds of commercial Doen-Jang, 5 kinds of home-made Doen-jang, and 16 kinds of Jeot-gal), 15 samples of Japanese fermented food (5 kinds of home-made soybean paste, and 10 kinds of Natto), and 19 samples of Indonesian fermented food (Tempe) as well as starters of Meju (500 microflora from Korea, and 22 from China). Initially, 11 isolates with strong fibrinolytic activity were selected for further characterization. The fibrinolytic activity of the 11 isolates ranged from 89 to 199% of standard plasmin. Four strains, M5l from Korean fermented food (Meju), I 1-1, I 1-4, and I 5-1 from Indonesian fermented food (Tempe), were chosen based on the degree of activity and reproducibility, and identified as Staphylococcus sciuri, Citrobacter or Enterobacter, Enterococcus faecalis, and Bacillus subtilis, respectively. The first two isolates are pathogenic stains while the latter two are considered as GRAS (Generally Recognized As Safe). Fibrinolytic activity of E. faecalis, characterized and designated as BRCA-5, reached a maximum, when the producer was cultivated in Ml7 broth supplemented with 1.0% glucose for 5 h at 37$^{\circ}C$ with shaking at 180 rpm. Compared to commercial fibrinolytic enzymes, the cell-free culture supernatant of 5. faecaiis BRCA-5 showed stronger activity than plasmin and streptokinase, but similar degree of specific activity as nattokinase and urokinase, aud it also demonstrated anticoagulant and antiplatelet activity ex vivo. These features of E. faecalis make it an attractive agent as a biomaterial for health-promoting foods.

• 한국미생물·생명공학회지
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• 제9권4호
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• pp.253-261
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• 1981

Fermented foods available in Korea may be classified into four groups, namely, fermented soybean products, fermented cereal products, fermented vegetable products, and fermented fishery products based on raw materials used. The representative fermented foods based on soybean are Kanjang(soysauce), Doenjang(fermented soybean pastes), and Kochujang(red pepper added-fermented soybean paste). Such fermented products are made using Meju(functioning as a starter and prepared by fermentation of steamed soybean mash) as an essential ingredient, and used widely as a soup base and/or in seasoning side-dishes for everyday meals year around. Excepting Sikhae, all fermented products based on rice and other cereal grains are of alcoholic nature. Takju (Makgeolli) used to be made using rice as the major raw material, however, mainly due to the shortage of rice in recent years, other cereals, such as, barely, corn, and wheat flours are also used to replace rice today. Owing to such changes in the raw materials. the popularity of Takju has been somewhat reduced, yet it is still widely consumed in rural areas. Although Chungju is a popular rice wine with superior qualify over Takju, the amount consumed is considerably limited. The highest qualify rice wine, Bupju, in particular, is made by a low temperature fermentation using glutinous rice. Kimchi is an unique fermented vegetable product of long tradition in Korea. Although it was for consumption mainly in winter season serving as a source of vitamins, today it is widely used throughout the year. Except Kkakdugi and Dongchimi, all of the fermented vegetable products contain salted Korean cabbage as an essential item, while they abound in varieties depending on material composition and methods of processing, and also on seasons and localities Next to Kimchi in this category is Kkakdugi made of raddish in popularity and quantity consumed. The four groups of fermented food products described above are reviewed in some detail and evaluated in terms of their nutritional significances, processes and microorganisms involved. and their commercial potentials. Jeotkal (or Jeot) is a name given to all fermented products of fishery origin. A number of Jeot can be prepared by adding salt and allowing fermentation to the raw materials such as shrimp, anchovy octopus, clam, oyster, etc.

• 한국식품영양과학회지
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• 제26권4호
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• pp.714-725
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• 1997

전통 발효식품의 소비정도와 마련방법를 알아보기 위해 대전지역 주부 290명을 대상으로 설문조사를 실시하였다. 김치류는 하루에 1번 이상 이용하는 주부가 97%, 매끼 마다 이용하는 주부는 87%로 가장 빈번하게 식탁에 오르는 발효식품이었다. 그 다음은 진간장, 국 간장, 고추장 및 된장의 순으로 하루에 1번 이상 이용한다는 답은 각각 47%, 46%, 31% 및 27% 이어서 장류는 우리 음식 준비시 매일 요구되는 발효식품임을 알 수 있었다. 식사 준비시 매우 낮은 빈도로 이용되는 발효식품은 청국장, 젓갈류 및 장아찌류로 거의 이용하지 않는다는 주부도 각각 18%, 19% 및 22%나 되었다. 특히 김치류, 장아찌류, 된장류, 고추장류, 젓갈류 및 청국장 등은 교육수준이 높고 젊은 주부일수록 소비빈도가 유의적으로 낮았다. 가족형태와 유의적 관계가 있는 발효식품은 된장류 뿐으로 대가족보다는 핵가족인 경우 소비 빈도가 낮았고, 주부의 직업 유무와는 젓갈류만 유의적 관련성이 있어서 일을 하는 주부들이 젓갈류를 자주 이용하고 있었다. 74%의 주부가 전통 발효식품의 소비가 감소하고 있다고 하였고, 감소 이유는 음식 종류의 증가(54%), 자녀들의 식성변화(27%), 반찬수의 증가(10%), 어른들의 식성 변화(9%)를 지적하였다. 김치류의 마련방법은 86%가 직접 제조한 것, 11%가 친척 집것을 이용하는 것으로 나타났고, 장류는 직접 제조하는 주부가 49% 뿐이었다. 교육수준이 높은 젊은 주부일 수록 직접 제조하지 않는 경향을 나타냈다(p<0.0001). 본 조사에서 얻어진 결과를 보면 교육 수준이 높은 젊은 주부일수록 발효식품을 식사 준비 시 적게 이용하는 경향을 나타내고 있다 따라서 식생활의 변화를 인정하되 전통 발효식품이 젊은 세대에 의해 지속적으로 소비되기 위한 프로그램을 개발하여 가정과 사회에서 실질적 교육이 이루어지도록 애써야 한다고 생각한다.

• 한국식생활문화학회지
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• 제31권5호
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• pp.411-429
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• 2016

This study introduced "Dongchundang Eumsikbeop" and examined its value as a reference. "Dongchundang Eumsikbeop" is a recipe book from the Head House of Eunjin Song Clan. As the author is unknown, the book is named after where it was found. "Dongchundang Eumsikbeop" records the recipes for 32 total foods, including 12 types of fermented foods, eight types of liquor, and six types of side dishes, etc. In "Jusiksiui", written by the same clan 100 years before, fermented foods account for 15% of its contents. On the other hand, this book assigns 34% of its space to fermented foods. It is assumed that the recipe book must have been compiled according to what households ate the most, as hostesses had to cook for their households due to financial difficulties at the time. In "Dongchundang Eumsikbeop", baking soda and alum were used as leavening agents for confectionery while sugar-based caramelizing was used for making soy sources, implying that modern food techniques were already applied. In short, this book provides a glimpse into the wisdom of hostesses of the Head House who improved recipes to suit changing times while adhering to tradition.

• 식품산업과 영양
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• 제21권2호
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• pp.36-39
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• 2016