• 한국가축번식학회지
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• 제23권3호
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• pp.183-189
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• 1999

본 연구는 강원도 평창군에 위치한 축산기술연구소 대관령 지소에서 사육된 한우 587두와 중국 연변지역 일반농가에서 사육된 연변황우 619두, 총 1,206두의 자료를 이용하여 모우산차, 송아지의 성별, 출생 년도 및 출생계절 등의 환경요인의 효과를 추정하였으며, 그 결과는 다음과 같다. 1. 한우와 연변황우의 임신기간의 최소자승평균치는 각각 287.3$\pm$0.60 및 282.3$\pm$0.80 일이었으며, 생시체중은 각각 24.9$\pm$0.25 및 25.2$\pm$0.24kg이었다. 2. 한우에서 임신기간과 생시체중은 산차가 증가할수록 유의적으로 증가하였으나 (p＜0.01) 연변황우는 유의성이 없었다. 3. 한우와 연변황우 모두 숫송아지 생시체중이 암송아지보다 더 무거웠고 (p＜0.01) 한우는 숫송아지 임신소의 임신기간이 암송아지 임신소의 임신기간보다 더 걸었으나 (p＜0.01)황우는 유의성이 없었다. 4. 생시체중에 대한 출생 년도의 효과는 한우와 연변황우에서 유의성이 있었으나 (p＜0.01) 임신기간에 대한 출생 년도의 효과는 유의성이 없었다. 5. 한우와 연변황우 모두 봄에 출생한 송아지가 제일 무거웠고 가을에 출생한 송아지가 제일 작았다 (p＜0.01).

• 한국수정란이식학회지
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• 제12권1호
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• pp.75-90
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• 1997

This study was carried out to establish the techniques for producing the calves of genetically superior Korean Native cattle by transfer of frozen-thawed embryos. The effects of some factors related to embryo recovery following superovulation and pregnancy rate following transfer of frozen-thawed embryos were evaluated. Also calving state was investigated. The results obtained were as follows ; The mean number of total and transferrable embryos recovered per superovulated cow was 8.72 and 4.90, respectively, from a total of 72 superovulations using 34 donor cows. There were no significant differences in the number of total or transferrable embryos recovered per superovulated cow between products of follicle stimulating hormone (FSH), years, seasons, and collection numbers. The pregnancy rate was found 44.44% following transfer of frozen-thawed embryos of Korean Native cattle to a total of 180 recipient cows including 82 Angus, 27 Charolais, 62 Hereford and 9 Korean Native cows. The pregnancy rate was significantly (P<0.05) higher in the transfer of excellent (42.99) and good embryos (40.17%), compared with fair (5.90%) grade embryos. And the pregnancy rate was significantly (P<0.05) higher in the transfer of embryos of morula stage (43.86%) than blastocyst stage (15.51%). But there were no significant differences in pregnancy rates between natural and induced estrus estrus asynchrony of 1 days, breeds, and parities of recipient cows. The normal calving rate of 80 pregnant cows following transfer of frozen4hawed em-bryos was 87.5% and the other 10 pregnant cows showed abortion during the period from pregnancy diagnosis at 50~60 days to calving. The average gestation length of normally delivered recipients was 288.50 days and the average birth weight of 70 calves born was 24.22 kg. The gestation length was significantly (P<0.05) shorter in the recipients delivering female calves (286.70 days) than males (289.39 days). But there were no significant differences in gestation tength and birth weight of calves born between the recipient breeds.

• 대한수의학회지
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• 제43권4호
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• pp.555-561
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• 2003

Incidence of estrum or abortions in pregnant cows may be affected by large follicles developed together with corpus luteum in pair ovaries of pregnant cows. But the follicles of pregnant phase were not assessed about histological findings. Determination of the healthy and atretic follicles by presence of proliferative cells or apoptotic cells and histological compositions of follicles would be used as important data on measurements of ovarian functions. This study was focussed mainly to investigate macroscopical, histological and immunohistochemical findings of ovarian follicles of pregnant Korean native cows and dairy cows (Holstein). In immunohistochemical methods, assessments of proliferative cells using PCNA antibody and apoptotic cells using TUNEL methods were performed. The follicles were observed on all 24 pregnant cows (17 Korean native cows and 7 Holstein cows). Follicles of greater than 10 mm in daimeter were developed in 37.5% (9/24 heads) of these pregnant cows. largest follicles from in these cows were $16.0{\times}15.0mm$ in diameter in a Korean native cow(l20 days of gestation), $13.4{\times}10.1mm$ in a Korean native cow(50 days of gestation), $12.9{\times}11.5mm$ in a Holstein cow (120 days of gestation). 40.5% among all follicles having diameter of greater than 1.0 mm in pregnant cows were assessed as atretic follicles and in addition, healthy follicles also showed less in number and smaller in size and thinner in wall layer compared with those of cyclic phase ovaries. In immunohistochemical findings, also proliferative positive cells and apoptotic positive cells on the granulosa cell layers in the healthy follicles of pregnant cows appeared less than on those of cyclic follicles. So these follicles were assessed as weakly active follicles. In large follicles, above positive cells were not nearly appeared but granulosa cell debris were more appeared among the granulosa cells. So these large follicles were assessed as inactvie or atretic follicles. The above findings suggest that small follicles of pregnant phase were weakly active or atretic and large follicles were inactive or atretic.

• 한국임상수의학회지
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• 제21권3호
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• pp.280-285
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• 2004

Pathological and bacteriological studies were made of a total of 111 udders from slaughtered Korean native cows originating from various locations in Chonnam province. Incidence of supernumerary teats was 8.1%. Gross lesions were found in 21.4% of mucous membranes of total teat cisterns; these lesions comprised epithelial hyperplasia (12.6%), congestion (4.1%), slight fibrosis (3.8%) and severe fibrosis with congestion (0.9%). Gross lesions in the glandular tissues were found in 35.1% of cows; these lesions comprised slight fibrosis (27.9%), moderate fibrosis (6.3%) and severe fibrosis with pus (0.9%). Mastitis was found only in one cow. One hundred and twenty-five strains of microorganisms were isolated from mammary gland fragments of the cows. Pathogens most commonly isolated were Staphylococcus spp.; most of them were minor mammary pathogens. Major mammary pathogens were Streptococcus spp. (9.6%) and E. coli (2.4%). Most of the isolates were susceptible to many of 13 antimicrobial agents used, but Streptococcus spp. and Gram-negative rods showed resistance to 7 and 8 agents, respectively. 97.5% of the isolates showed susceptibility to amoxicillin, but only 55.8% to erythromycin.

• 한국가축번식학회지
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• 제22권4호
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• pp.359-366
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• 1998

본 조사연구는 산차에 따른 한우 번식우의 초종부, 초임 및 초산일령과 분만후 발정재귀일수, 수태당 종부회수, 분만후 수태일수, 분만간격 등을 검토하기 위하여 1996년 1월부터 1997년 12월까지 20농가에서 사육중인 한우 성빈우 670두를 대상으로 조사를 수행하였다. 1. 한우 미경산우의 초종부일령은 평균 443.0일이었고 초임일령은 평균 457.0 일이었으며 초산일령은 평균 746.6 일이었다. 2. 산차에 따른 분만후 발정재귀일수는 평균 70.1일이었고 산차별로는 2산차, 3산차, 4산차, 5산차 이상이 각각 70.5일, 66.4일, 79.7일, 60.4일로 5산차 이상이 가장 빨랐고 (p＜0.05), 4산차가 가장 늦은 경향을 나타내었다 (p＜0.05). 3. 산차에 따른 수태당 종부회수는 평균 1.53 회였고 미경산우, 2.산차, 3산차, 4산차 및 5산차가 각각 1.56 회, 1.43회, 1.48회, 1.67회 및 1.73회로 2산차가 가장 적었고 (p＜0.005) 5산차 이상이 가장 많았으며 (p＜0.05) 미경산우를 제외하고는 산차가 증가함에 따라 수태당 종부회수가 증가하는 경향을 나타내었다. 4. 산차에 따른 분만후 수태일수는 평균 91.2 일이었고 산차별로는 2산차, 3산차, 4산차, 5산차 이상이 각각 86.9일, 89.6일, 104.5일 및 107.0일로 산차간에 유의적인 차이는 인정되지 않았으나 (p＞0.05) 대체로 산차가 증가함에 따라 길어지는 경향을 나타내었다. 5. 산차에 따른 분만간격은 평균 375.3 일이었고 산차별로는 2산차, 3산차, 4산차 및 5산차 이상이 각각 370.8 일, 371.0일, 395.2일 및 383.9일로 2산차가 가장 짧았고 (p＜0.05) 4 산차가 가장 긴(p＜0.005) 경향을 나타내었다.

• 한국가축번식학회지
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• 제12권1호
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• pp.20-23
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• 1988

The 3211 Korean native cows raised in Muan, Milyang were investigated for the purpose of finding reproductive rate of cows that retained fetal membrane for two years from April, 1981 to March, 1983. The results obtained were summarized as follows ; 1. The incidence of retained fetal membrane was found in 64 cows(1.98%). 2. The day to first estrus was similar to normal postpartum period unless concurrent genital infection occured. 3. Conception rate of cows that retained fetal membrane was 32.7%, 10.9%, 5.2% and 3.6% at the first, second, third and forth service, respectively and non pregnant cow was 47.6%. 4. Reproductive disorder of non pregnant cows was the highest in repeat breeder and the effect of treatment was very low.

• 한국수정란이식학회지
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• 제15권2호
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• pp.147-156
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• 2000

To investigate fatty acid constituents and relative compositions in the fluid of the follicles, oviducts, uterine body and uterine body in Korean native cow, the fluids of the reproductive tract were analyzed using gas chromatography. The samples were taken from various reproductive tract of 23 Korean native cows. q. Caprylic acid (C8:0), myristic acid(C14:0), palmitic acid(C16:0), palmitoleic acid(C16:1), stearic acid(C18:0), oleic acid(C18:1), linoleic acid(C18:2), arachidonic acid(C20:4) were found in the reproductive tracts of the cows, which made 8 kinds of fatty acid in total. 2. Palmitic acid, oleic acid, stearic acid were predominant with 35.67%, 24.98% and 17.52%, respectively. while low levels of fatty acids(<5%) were myristic acid, palmitoleic acid and caprylic acid with 1.75%, 1.28% and 2.69%, respectively. 3. Two kinds of polyunsaturated fatty acids, linoleic acid arachidonic acid were found in the reproductive tracts of cows. 4. Palmitic acid among saturated fatty acids and oleic acid among unsaturated fatty acids were the highest level in all of the reproductive tracts. 5. The Highest level of arachidonic acid was found in the uterine horn. 6. The sum of the palmitic acid and oleic acid were 61.72%, 63.72%, 57.66% and 57.65% for the fluid of follicle, oviduct, uterine horn and uterine body of the cows, respectively. 7. The relative compositions of palmitic acid, palmitoleic acid and caprylic acid were higher during the luteal phase than during the follicular phase. 8. The relative compositions of arachidonic acid was higher during the follicular phase in the fluid of uterine horn and uterine body of the cows. 9. The long chain fatty acid, the palmitic acid, stearic acid, oleic acid and linoleic acidshowed higher relative compositions during the follicular phase(86.49%∼95.51%) than during the luteal phase(85.64%∼88.93%).

• 한국임상수의학회지
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• 제15권2호
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• pp.450-454
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• 1998

These studies were performed to find out the possibility of automatic detection of the diseased animal with fever by farmers themselves. Firstly, the body temperature of 331 dairy cows was investigated according to major disease symptoms manifested. Secondly, AD 590 thermometer was used to take the teat temperature of the milking cows to determine the possibility of automatic taking of body temperature while milking. The temperatures of scapha of ear and coccygeal artery part were also taken fur the non-milking dairy cows and Korean native cowl 1. The average body temperature of dairy cows associated with respiratory diseases puerperal disease, or mastitis was higher than normal temperature denoting respectively 39.8,39.6, and $39.3{\circ}C.2.$ The teat temperaure of the milking dairy cows with fever($39.5~39.6{\circ}C$) and the cows with mastitis was respectively 1.02 and 0.56${\circ}C$ higher than that of normal cows. 3. The average teat temperature taken by AD 590 was 33.91, 34.93, and 34.50${\circ}C$ in normal milking dairy cows, cows with fever(39.5~39.6${\circ}C$), and cows with mastitis, respectively. 4. The mean temperatures at scapha and coccygeal part of non-milking dairy cows and Korean native cows were 35.62 and 36.63${\circ}C$, respectively. It was concluded that AD 590 thermometer would be usable for the farmers to automatirally detect the body temperature of dairy cows while milking and subsquently to find the diseased cow with fever and that the scapha of ear and coccygeal artery part of the cattle could be the body parts of simply detecting body temperature of non-milking cattle.

• 한국가축번식학회지
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• 제18권1호
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• pp.47-54
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• 1994

The objective of this study was to produce Korean native calves following transfer of in vitro matured, fertilized and cultured embryos into Holstein cows. The ovaries of Korean native cows or heifers were obtained from an abattoir and kept on 25 to 28$^{\circ}C$ and transported to laboratory within 2 hrs. The oocytes were matured in vitro (IVM) for 24 hrs in TCM-199 supplemented with 35$\mu\textrm{g}$/ml FSH, 10$\mu\textrm{g}$/ml LH, 1$\mu\textrm{g}$/ml estradiol-17$\beta$ and granulosa cells at 39$^{\circ}C$ under 5% CO2 in air. They were fertilized in vitro (IVF) by epididymal spermatozoa treated with heparin for 24 hrs., and then the zygotes were co-cultured in vitro (IVC) with bovine oviductal epithelial cells for 7 to 9 days. Late morulae and blastocysts produced in vitro were nonsurgically transferred to recipient cows by unilaterial. Recipients were monitored for estrus and for pegnancy by rectal plapation in 60 days after embryo transfer. One of them was pregnant to term and produced a female weighing 42.5kg at birth.

• 농업과학연구
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• 제47권3호
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• pp.667-672
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• 2020

It is well known that intensive selection caused a decline in reproductive performance in dairy cattle. Interestingly, the reproductive performances including fertility and calving interval of Korean native beef cattle have declined in the last 20 years, suggesting that a breeding program focusing on carcass weight and intramuscular fat may affect the reproductive physiology in Korean native beef cattle, too. In this study, we analyzed the calving interval (CI) and selection index (SI) based on genome-wide association studies (GWAS) of Hanwoo cows for seven years (2013 - 2019). Multiparous cows (4.5 ± 0.11) were analyzed, which were bred by artificial insemination (AI). We first examined the distribution of the AIs and calving dates. About 40% of the AIs were carried out in May to June and October to December; subsequently, calving was observed from March to April and August to October, respectively, indicating the cows were seasonally bred. No correlation between CI and SI was found (y = 0.0459x - 17.64; R² = 0.0356), but the ratio of cows with a positive SI was higher in the longer CI group compared to the shorter group, suggesting that the selection for meat quality and quantity may affect the reproductive performances. In addition, the average value of SI was - 3.42 in the CI < 400 while + 5.79 in the CI > 400 although the values were not statistically significant. However, our results suggest that reproductive indices such as fertility and CI should be considered for sustainability in the Hanwoo breeding selection program.