• Journal of Korean Society of Forest Science
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• v.100 no.3
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• pp.441-448
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• 2011

The objective of this study was to clarify the distribution, host preference and habitat characterstics of the endangered mistletoe species, Loranthus tanakae in Korea. Seven sites were selected for sampling in Gangwon Province, located in middle of Korea. Parameters including DBH, attached height, twig death, and the number of L. tanakae and Viscum album var. coloratum per tree for all infected individuals were investigated. One thousand, two hundred and seventy L. tanakae plants were found on the 420 host trees with 665 V. album var. coloratum on 227 of the trees. The infested host trees belonged to eight species and one variety species from six genera and five families. The highest percentage (86.9%) of individuals of L. tanakae were growing on Fagaceae. The infected trees occurred between altitudes of 420 m and 1,250 m above the sea level, but most of them were between 600 m and 900 m. The mean DBH and heights of parasitism were 38.6 cm and 13.0 m, respectively. Twig death was also observed on 300 host trees (71.4%) and 1.5 twig per tree. Most of the populations and the distribution of L. tanakae were highly affected by DBH out of some individual tree characteristics.

• Journal of Aquaculture
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• v.22 no.1
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• pp.56-62
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• 2009

Effects of Korean mistletoe extracts (KM-110), Viscum album coloratum on the specific and non-specific immune responses of Japanese eel Anguilla japonica were examined. The optimal concentration not showing toxicity of KM-110 was determined to $30-40{\mu}g/ml$ in vitro and $100{\mu}g$/100 g of fish in vivo. Even $1000{\mu}g$ of KM-110/100 g of fish did not show any clinical problem in fish though the levels of toxic parameters were slightly increased. In terms of antibody production, KM-110 significantly elicited more antibody production than FCA or $\beta$-glucan. $\beta$-glucan plus KM-110 group synergistically enhanced antibody production. There was no significant difference between KM-110 and KM-110 plus $\beta$-glucan group. The ROI production by head kidney (HK) leucocytes of eel injected with 500 or $1000{\mu}g$ KM-110 was significantly (P<0.05) enhanced than the control and FCA-treated group. Maximum increase in the NBT reduction value was observed in $1000{\mu}g$ KM-110 group but no significant difference was found between 500 and $1000{\mu}g$ KM group. The level of serum lysozyme activity was significantly (P<0.05) higher in the 500 and $1000{\mu}g$ KM-110- or FCA-treated group than in the control and $200{\mu}g$ KM-110 group. The phagocytic activities of HK leucocytes isolated from eel injected with 500 and $1000{\mu}g$ KM-110 were significantly (P<0.05) higher than $200{\mu}g$ KM-110 and PBS-injected control group. Korean mistletoe appeared to be a good activator of the specific and non-specific immune responses of Japanese eel.

• Korean Journal of Plant Resources
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• v.22 no.5
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• pp.389-393
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• 2009

Regional distribution of mistletoe (Viscum album var.coloratum) and its habitual environments were investigated in order to obtain the basal data on the artificial propagation to cope with its increasing consume for medicine. Mistletoes inhabited throughout the overall region of the South Korea investigated. They were parasitic mainly to the Quercus spp. including Q. serrata and rarely to the Castanea crenata var. dulcis, Prunus serrulata var. spontanea, Alnus japonica, and Pyrus pyrifolia, etc. Mistletoes were not observed on the conifers such as Pinus densiflora and Pinus koraiensis and some deciduous broad-leaved trees species such as Zelkova serrata, Diospyros kaki, Acer mono, Acer palmatum, and Morus alba. Their habitats were located from zero to 1,200 m above sea level nevertheless the direction or slope of the mountains, suggesting that artificial propagation can be carried out nation widely to the well-grown parasite tree species. Parasitic specificity related to the physical and chemical characteristic of the epidermal tissues will be studied further.

• Journal of Korean Society of Forest Science
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• v.102 no.3
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• pp.428-436
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• 2013

To obtain biological basic data for the conservation strategies establishment of a rare mistletoe species, Loranthus tanakae(Loranthaceae) in Korea, the distribution range, habitat characteristics and an assessment of the conservation status on the natural populations of L. tanakae were investigated. As a result, the natural populations of L. tanakae were distributed in Bakdudaegan from Mt. Chiri to Mt Seorak in Korea penninsula, and 97.8% of surveyed individuals of the mistletoe species were found in Gangwon province. In natural populations, 1,385 individuals of L. tanakae were parasitic on 480 host trees, and distributed in sunshiny ridges of mountains of altitude range of 353 m to 1,250 m. The range of host trees of L. tanakae were composed of 5 families, 6 genera, 9 species, 1 subspecies, and of these, Quercus mongolica was preferred with 81.5% (389 trees among 480 host trees). As a result of assessing the conservation status through IUCN, L. tanakae was evaluated as Vulnerable (VU). It was considered that Mt. Seorak, Mt. Taegi and Mt. Odae population as habitats with the highest density of distribution of natural populations of L. tanakae should be designated as the protected areas. Thus, conservation strategies and related methods of the natural populations of L. tanakae were also discussed for the sustainable conservation.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.253-253
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• 2005

• Proceedings of the Korean Society of Life Science Conference
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• 2007.10a
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• pp.51.1-51.1
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• 2007

See Full Text

• Proceedings of the PSK Conference
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• 2001.04a
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• pp.203.2-204
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• 2001

• Proceedings of the PSK Conference
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• 2001.10a
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• pp.256.3-257
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• 2001

• Journal of the Korean Wood Science and Technology
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• v.24 no.1
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• pp.87-94
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• 1996

한국산 동백나무겨우살이(Pseudixus japonicus) 추출물의 암치료를 위한 생약으로서 활성 유효성을 검증하기 위하여 메탄올, 석유에테르, 클로로포름, 초산 에틸 용매로 순차적으로 추출하여 겨우살이 추출물의 다섯 가지 분획을 얻어, 이에 대하여 in vitro로 1차와 2차 검색 시스템을 사용해 항암활성 성분을 체계적으로 검색하였다. 다섯 가지 분획 중 클로로포름 가용성 분획이 1차 검색 세포인 $P388D_1$에 대해 가장 높은 항암활성을 나타내어 MSB1, NIH/3T3, SNU-1, SNU-C2A 등 2차 검색 시스템에 대해 클로로포름 가용성 분획의 항암활성을 다양한 농도하에서 비교 검색하였다. 혈액암 세포중 특히 $P388D_1$의 생장이 클로로포름 추출물에 의해 강하게 저해되었으며, 형질전환된 생쥐의 태아 섬유아세포와 사람의 대장암, 위암세포들도 어느 정도의 생육저해를 나타내었다. 이 클로로포름 가용성 분획의 주성분은 원소분석, 발색시약과의 반응, IR, GC-MS, $^{13}C$-NMR의 스펙트럼의 결과로 세 종류의 알칼로이드 화합물로 확인되었고, 부성분으로는 지방산 메틸 에스테르와 프탈라이드 화합물이 MS 스펙트럼을 통해 동정되었다.

• Applied Biological Chemistry
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• v.47 no.2
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• pp.279-282
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• 2004

An antioxidant was isolated from Korean mistletoe (Viscum album var. coloratum) by consecutive purification using silica gel, Sephadex LH-20, and RP-HPLC. The active principle was identified as homo-flavoyadorinin-B (3',7-dimethoxyluteolin-4'-O-[apiosyl $(1{\rightarrow}2)$ glucoside]) by spectral analyses. It inhibited 74.6% of hydroxyl radical and 30.6% of superoxide anion radical at 0.01 mM; however, th~compound did not show any scavenging activity against hydrogen peroxide radical. At 0.1 mM, above compound scavenged superoxide anion radical about twice as effective as positive controls, BHT and ${\alpha}-tocopherol$. Radical scavenging activities of homo-flavoyadorinin-B on DPPH, hydroxyl, and hydrogen peroxide radicals were almost same with those of positive controls.