Kim, Jong-Rae;Jung, Chang-Ho;Kim, Yong-Ho;Yoon, Jong-Man
Development and Reproduction
/
v.10
no.4
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pp.227-238
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2006
Genomic DNAs(gDNAs) were isolated from the venus clam(Gomphina aequilatera) from Samcheok(venus clam from Samcheok; VCS) and Wonsan(venus clam from Wonsan; VCW) located in the East Sea of the Korean Peninsula. The amplified products were generated by agarose gel electrophoresis(AGE) with oligonucleotides primer, detected by staining with ethidium bromide and viewed by ultraviolet ray. The seven arbitrarily selected primers BION-21, BION-23, BION-25, BION-27, BION-29, BION-31 and BION-33 generated the shared loci, polymorphic, and specific loci, with the molecular sizes ranging from 150 bp to 2,400 bp. In this study, 147 polymorphic loci(147/954 loci, 15.41%) in VCS population and 274(274/996 loci, 27.51%) in VCW population were generated with seven primers. These results suggest the genetic variation in VCW population is higher than in VCS population. Especially, the 700 bp bands generated by the primer BION-21 were identified commonly in two Gomphina populations, which identified populations and/or species. This specific primer was found to be useful in the identification of individuals and/or population, resulting from the different DNA polymorphism among individuals/species/population. Two Gomphina populations between the individual SAMCHEOK no. 03 and WONSAN no. 22 showed the longest genetic distance(0.696) in comparison with other individuals used. The complete linkage cluster analysis indicating three genetic groupings and dendrogram revealed close relationships among individual identities within two geographical populations of venus clam(G. aequilatera) from the Samcheok and Wonsan. The intra-species classification and clustering analyses inferred from molecular markers supported the traditional taxonomy of the species based on morphological characters such as shell size, shape and color. Accordingly, as mentioned above, RAPD analysis showed that VCS population was more or less separated from VCW population.
Korean Journal of Agricultural and Forest Meteorology
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v.8
no.1
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pp.36-44
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2006
In this study, we constructed a forest fire danger map in the Yeongdong area of Gangwon-do and Northeastern area of Gyeongsangbuk-do using a forest fire rating model and geographical information system (GIS). We investigated the appropriate positions of the automatic weather station (AWS) and a comprehensive network solution (a system including measurement, communication and data processing) for the establishment of an optimum mountain meteorological observation network system (MMONS). Also, we suggested a possible plan for combining the MMONS with unmanned monitoring camera systems and wireless relay towers operated by local governments and the Korea Forest Service for prevention of forest fire.
Pleurotus spp. have been used for edible and medicinal purposes in Asian countries for a long time. The fruiting bodies of the Pleurotus ostreatus, Pleurotus citrinopileatus and Pleurotus salmoneostramineus contained many physiologically beneficial substances for human health. Therefore, it is necessary to study the genetic diversity of Pleurotus mushroom cultivars commercially cultivated in Korea. Eleven strains of Pleurotus spp. were collected from different geographical regions in South-East Asia and ITS regions of rDNA and RAPD of genomic DNA were analyzed. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 167 to 254 bp and 156 to 213 bp, respectively. The sequence of ITS1 was more variable than that of ITS2, and the 5.8S sequences were identical. A phylogenetic tree based on the ITS region sequences indicated that selected strains could be classified into 4 clusters. Eleven Pleurotus species were also analyzed by RAPD with 20 arbitrary primers. Ten of these primers were efficiently amplified the genomic DNA. The number of amplified bands varied with the primers and strains, with polymorphic fragments in the range from 0.1 to 2.0kb. The results revealed that genetic diversity of selected strains of P. ostreatus, P. citrinopileatus and P. salmoneostramineus is low.
Prunus sargentii complex of subgenus Cerasus is an Eastem Asiatic plant group that exhibits a broad range of morphological variation and includes P. takesimensis, P. yedosensis, P. verecunda, and P. sargentii. In this study, a morphological analysis was undertaken to determine whether the observed morphological variation was primarily attributable to morphological discontinuities among the taxa. P. sargentii, which distributed eastem area in Korea, northern area in Japan and far east Russia had umbel like inflorescence and additionally was characterized by sticky bud and leaf twigs, compared with P. serrulata complex. Also, P. verecunda in Korea and Japan was characterized by umbel like inflorescence and presence of hair in leaf, petiole and pedicel, and was treated as a variety of P. sargentii. Evidence obtained from multivariate morphometric analyses indicated that the entity of P. takesimensis formed a cohesive group somewhat distinct from P. sargenti.. Especially, P. takesimensis was characterized by relatively small flowers (26-32mm in diameter) and many flowers [(2)3-5] per umbel inflorescence, compared with P. sargentii (34-48mm and 2(3) per inflorescence) and should be recognized as an independent and endeImic taxon in Korea. Additionally, P. yedosensis, which was known to have umbel inflorescence (short peduncle type) with pubescent style based on the type specimen, was comprised of corymb inflorescence (long peduncle type) as well. The morphological differentiation between these two types of P. yedosensis was not considered sufficient to warrant recognition of specific status because of the putative hybrid origin, no distinctive geographical distribution pattern, and existence of various peduncle length on Island Jeju-do of Korea.
An area's topography refers to the shape of the earth's surface, described by its elevation, slope, and aspect, among other features. The topographical conditions determine energy flowsthat move water and energy from higher to lower elevations, such as how much solar energy will be received and how much wind or rain will affect it. Another common factor, the topographic wetness index (TWI), is a calculation in digital elevation models of the tendency to accumulate water per slope and unit area, and is one of the most widely referenced hydrologic topographic factors, which helps explain the location of forest vegetation. Analyses of topographical factors can be calculated using a geographic information system (GIS) program based on digital elevation model (DEM) data. Recently, a large number of free open source software (FOSS) GIS programs are available and developed for researchers, industries, and governments. FOSS GIS programs provide opportunitiesfor flexible algorithms customized forspecific user needs. The majority of biodiversity in island areas exists at about 20% higher elevations than in land ecosystems, playing an important role in ecological processes and therefore of high ecological value. However, island areas are vulnerable to disturbances and damage, such as through climate change, environmental pollution, development, and human intervention, and lacks systematic investigation due to geographical limitations (e.g. remoteness; difficulty to access). More than 4,000 of Korea's islands are within a few hours of its coast, and 88% are uninhabited, with 52% of them forested. The forest ecosystems of islands have fewer encounters with human interaction than on land, and therefore most of the topographical conditions are formed naturally and affected more directly by weather conditions or the environment. Therefore, the analysis of forest topography in island areas can be done more precisely than on its land counterparts, and therefore has become a major focus of attention in Korea. This study is focused on calculating the performance of different topographical factors using FOSS GIS programs. The test area is the island forests in Korea's south and the DEM of the target area was processed with GRASS GIS and SAGA GIS. The final slopes and TWI maps were produced as comparisons of the differences between topographic factor calculations of each respective FOSS GIS program. Finally, the merits of each FOSS GIS program used to calculate the topographic factors is discussed.
The aim of this paper is analysis of structure and development pattern about wooden coffin and chamber tombs in Gyeongju from the 2nd century B.C. to the 3rd century A.D. for researching to socio-political tendency and growth process of Sarokuk. Tombs buried with iron objects were built in Youngnam(嶺南) from the 2nd century B.C. with spread wooden coffin with stone mound(積石木棺墓). Also medium or small sized wooden coffin tombs buried with bronze mirror of western Han(前漢) and soft stoneware(瓦質土器) were appeared the 2nd century B.C. in Gyeongju, because of establishment of Han's commanderies(漢郡縣) in the Korean Peninsula and refuge from Daedong river(大同江) to Jinhan(辰韓). Separate tombs(獨立墓) with lots of bronze object ware assumed high ranked tombs of parsonage(司祭王) or local chief(地域首長). From the 2nd century A.D. the size of wooden coffin tombs became enlarged and funerary objects ware abundant, for example Sarari 130th tomb(舍羅里 130號). The burying pattern of this tomb is similar to wooden chamber tombs in Lelang(樂浪), which had prestige goods like lacquer ware and bronze mirror in wood box(木匣) beside coffin. Appearance of these wooden chamber tombs that were different from original wooden coffin tombs imply interaction between Lelang and these area with iron. Sarari community that held right of trade and distribution to outside through the geographical advantage grew up centered position in Gyeongju politically, socially, and culturally. Chamber in tomb as a new structural notion that can secure funerary objects became firmly was established from the 2nd century A.D. in Gyeongju and large sized wooden chamber tombs were generally built early of the 3rd century A.D. This tendency was reflected in stratification of community and growth as center of local state. After late of the 3rd century A.D. Gyeongju type wooden chamber tomb(慶州式木槨墓) which had subordinate outer coffin(副槨) was appeared and then subordinate outer coffin was as bigger as main chamber(主槨) the 4th century A.D., because of centralization and stratification in society and unification of various communities among the Gyeongju area.
Hong, Jin Young;Lee, Jeung Min;Kim, Young Hee;Kim, Soo Ji;Jo, Chang Wook;Park, Ji Hee
The Korean Journal of Mycology
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v.47
no.2
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pp.131-142
/
2019
The Bupjusa and Seonamsa temples are located at places with geographically different condition, and therefore, differ with respect to building's structure and layout. In addition, evident difference can be appeared by the regional climate. For the 2 years, we studied the concentration and diversity of the seasonal airborne fungi inside and outside of the 2 temples. In Seonamsa temple, airborne fungi concentration in the indoor and outdoor air was higher and the species diversity in the indoor air was lower, whereas, concentration variation was larger than that of Bupjusa temple. A total of 173 fungal isolates (including 54 genera) and 162 isolated (including 49 genera) were obtained from the indoor air of Bupjusa and Seonamsa temple, respectively. Whereas, 80 fungal isolates (including 33 genera) and 74 isolates (including 39 genera) were collected form the outdoor air of Bupjusa and Seonamsa temple, respectively. However, more fungal varieties were observed to be distributed inside Bupjusa and outside Seonamsa temples. Amongst all the fungi identified, ascomycetes were more dominant (plus or minus 90% points), followed by basidiomycetes and zygomycetes; which more presented in outdoor air than in indoor air. The airborne fungi concentration in spring (month of April) and autumn was higher than in any other season, for Seonamsa and Bupjusa temples, repectively. Genus Cladosporium was isolated from each site and season, with its dramatic increase noted in autumn. In addition, the highest basidiospore(s) number was obtained after the rain. Consequently, the results suggest that Seonamsa temple was more susceptible to biological damage than Bupjusa temple was.
Kim, Yonggyun;Kim, Hyoil;Mollah, Md. Mahi Imam;Al Baki, Md. Abdullah
Korean journal of applied entomology
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v.58
no.2
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pp.133-142
/
2019
This study analyzed genetic variation of the Oriental fruit fly (OFF), Bactrocera dorsalis, which is designated to be a quarantine insect pest in Korea. OFF samples endemic to Taiwan were collected at three different locations (Taipei, Taichung, and Kaohsiung) for three days from July 30 to August 1 in 2018 and assessed in their age and mitochondrial DNA sequence variations. In these places, 1,085 OFF males were collected using methyl eugenol lure while 30 males of Zeugodacus cucurbitae and one male of Bactrocera tau were collected using Cuelure. A protein diet lure attracted 6 flies including one OFF and 5 flies of Z. cucurbitae. Male heads of OFF contained pterin, which increased in contents with age from 32 to $59{\mu}g/head$. There was a local variation in pterin amounts in OFF heads, in which Kaohsiung population had lower amounts of pterin than Taipei and Taichung populations. Genetic distance among these three populations were measured by random amplified polymorphic DNA and showed that Taipei population was separated from Taichung/Kaohsiung cluster. Genetic variation was also analyzed in sequence variations in cytochrome oxidase I (CO-I) and NADH dehydrogenase I (ND-I). There was 7.8% variation in CO-I sequence (360 residues) and 6.6% variation in ND-I sequence (213 residues). These polymorphic sites are proposed to be used to develop SNP (single nucleotide polymorphism) markers characteristic to Taiwan OFF populations.
The purpose of this study is to understand the vegetation characteristics of ridges (Gyeongsusan-Seonunsan-Gaeipalsan) in the Seonunsan Provincial Park and to establish reference information for the management of the park in the future. We designated 62 plots with the area of $100m^2$ were installed and analyzed them to investigate the vegetation characteristics. The results of community classification based on TWINSPAN showed seven categories of vegetation communities in the surveyed region: Quercus dentata-Deciduous broad-leaved Community, Quercus variabilis-Pinus thunbergii-Quercus serrata Community, Pinus densiflora Community, Deciduous broad-leaved Community-I, Carpinus tschonoskii-Castanea crenata-Quercus aliena Community, Deciduous broad-leaved Community-II, and Carpinus tschonoskii-Carpinus laxiflora Community. In the vegetation of Seonunsan Provincial Park, coniferous trees such as Pinus thunbergii and Pinus densiflora have been gradually losing their population as part of ecological succession to deciduous broad-leaved trees such as Quercus spp., Carpinus tschonoskii, and Carpinus laxiflora. Moreover, Carpinus turczaninowii, Mallotus japonicus, and others were identified as vegetation reflecting the geographical characteristics of the region neighboring the west coast. The estimated age is 30-60 years, and the oldest tree Pinus densiflora is 63-years old. The index of diversity ($100m^2$) was 0.7942 for Carpinus tschonoskii-Carpinus laxiflora Community, 0.8406 for Carpinus tschonoskii-Castanea crenata-Quercus aliena Community, 0.8543 for Quercus dentata-Deciduous broad-leaved Community, 0.9434 for Quercus variabilis-Pinus thunbergii-Quercus serrata Community, 0.9520 for Deciduous broad-leaved Community-I, 0.9633 for Pinus densiflora Community, and 1.0340 for Deciduous broad-leaved Community-II in the ascending order.
This study is to analyze the current status of transmission and musical characteristics of Naeseo-deulilsori in Changwon, and the purpose of this study is to illuminate the value of deulilsori in Naeseo region, so that it can be continuously inherited. Naeseo-deulsori consists of the Mosimgi-sori, Nonmaegi-sori, and Chingchingi-sori. Mosimgi-sori, which is called when planting a seedling, is divided into 6 types according to the order and situation of work. ① rice planting sound, ② rice planting sound called in the morning, ③ rice planting sound called at lunch time, ④ rice planting sound called after lunch, ⑤ rice planting sound called when it is raining or cloudy, ⑥ rice planting sound called at sunset. Mosimgi-sori, which is called when planting a seedling, is based on Menali-tori, but partly influenced by Yugjabaegi-tori. However, it was typical Menali-tori in the slow The sound of rice planting in the nearby Haman region, but as the speed increased in the fast The sound of rice planting, the characteristics of Menali-tori faded and la↘mi perfect fourth descending frequently appeared. In the sound of rice planting in Goseong, both slow and fast sounds were strongly influenced by Yugjabaegi-tori. In the end, the sound of rice planting in the Naeseo region is less Yugjabaegi-tori than in the Goseong region and stronger than in Haman region. This combination of tori is a musical bargaining phenomenon that appears in the border region, and it can be said to be a geographical and regional characteristic of the Naeseo region. Nonmaegi-sori has the same sound as 'Sangsadeio' throughout the nearby Goseong and Haman regions. However, in Nonmaegi-sori in the Naeseo region, a strong Gyeong-tori tendency is found in the sound received. Looking at the flow of the melody of Nonmaegi-sori, it seems that the pitch has been changed by the intestines in recent years, and this modified melodic form has continued as it is. In order to guarantee locality, this part seems to need to be corrected in the future.
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