• Journal of fish pathology
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• v.23 no.3
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• pp.389-398
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• 2010

Effects of various concentration of yuzu Citrus junos Siebold ex Tanaka in the diets on growth, blood chemistry and disease resistance of olive flounder were determined. Fifteen hundred fish averaging 200~270g were fed on moist pellet containing yuzu at the concentrations of 0%(control), 2.5%, 5% and 7.5% for 3 months. After feeding trial, weight gain of fish fed 2.5% and 5.0% yuza diet was higher than those of fish fed 0% and 7.5% yuza diet but not significant(P>0.05). No differences in hematological and physiological indices of olive flounder were found among the experimental diets except for total cholesterol. Serum lysozyme activity was not significantly different among fish fed experimental diets but bactericidal activity of fish fed 7.5% yuza diet was significantly different from those of fish fed 0, 2.5 and 5% Yuza diets. Survival rates of fish fed 2.5% yuza diet at experimental infection with Edwardsiella tarda and Streptococcus sp. were higher than those of the other experimental groups but not significant. In conclusion, Dietary inclusion of 2.5~5% yuzu seems to have positive effects for fish health condition and disease resistance.

• Journal of fish pathology
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• v.23 no.2
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• pp.211-219
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• 2010

Specimens were collected from the coastal region near industrial complex of Ulsan-Onsan, Sihwa-Ansan and Yeosu-Gwangyang in 2008 and 2009. The total number of individuals used in analysis was 1,289 of Acanthogobius flavimanus, Chelon haematocheilus, Hemibarbus labeo, Leiognathus nuchalis, Mugil cephalus and Synechogobius hasta. The sex ratio in the total individual was 1:0.73 (female:male). Specific sex ratio of fishes in the areas, namely the Ulsan-Onsan, Sihwa-Ansan and Yeosu-Gwangyang were 1:0.79, 1:0.81, and 1:0.25, respectively. Especially, female in Yeosu-Gwangyang was higher than male. The intersexuality in the total individual was 11.7%. Intersexuality of fishes in the areas were 4.98, 14.39 and 25.0% in the Ulsan-Onsan, Sihwa-Ansan and Yeosu-Gwangyang, respectively. It was indicated female higher than male in Ulsan-Onsan and male higher than female in Sihwa-Ansan and Yeosu-Gwangyang.

• Journal of fish pathology
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• v.31 no.1
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• pp.9-13
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• 2018

We previously monitored nervous necrosis virus (NNV) in brain samples of artificially produced walleye Pollock (Gadus chalcogrammus) seedlings, with a low prevalence (1.8%, 1/55) but no clinical symptoms. Given that this virus is considered one of the most serious viral threats for almost all marine aquaculture fish species and characterized by both vertical and horizontal transmission, it would be interesting to monitor NNV in the fertilized eggs as well. We collected fertilized walleye pollock eggs from the farms located in Goseong during January to March, 2017. Approximately 50 mg of eggs were periodically taken from 4 each different batches, and 37 different pooled sample sets in total were made during sampling period. RNA was extracted from the eggs by using Trizol and cDNA was synthesized for RT-PCR for detecting NNV. Primers and PCR conditions are the same as previously described. As a result, NNV was not detected from any of the sample sets by one step PCR (0%, 0/37), suggesting NNV may not be a threat in walleye pollock aquaculture in Korea at present time. However, continuous monitoring for NNV should be conducted because introducing a new species into aquaculture industry involves potentials of disease outbreak and NNV is already known to cause outbreaks in gadoid fishes.

• Journal of Navigation and Port Research
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• v.32 no.6
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• pp.499-503
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• 2008

As the conference result of MEPC in IMO, development of ballast water management system corresponding to newly established ballast water management standard (D2 regulation) of shipping is being made an active progress over the whole world. The ballast water management system should treat particular material of more than $10{\mu}m$ in high capacity of more than 500t per hour in the event of filtration process. Also, it is very difficult to develop a practical management system since a limited element which a narrow space of the ship should be designed in the minimal volume is assumed. Therefore, the study promoted a study on the next generation auto back wash media filter to overcome such a limited element. Also, the study performed pressure and flux measurement test followed by thickness of each filter medium for filtration by each size to grasp a relation between absorption and pressure at the time of vacuum filtration and mechanical analysis and turbidity change observation of filtered water after vacuum filtration.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.285-290
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• 2009

This experiment was conducted to investigate the propagation system by seed to check growth, flowering, and making seed of three-year-old cactus of potted Mammillaria goldii. M. theresae, and M. pseudopeclinata. Plant height and width of them was 2.5~2.8 cm, and 2.8~3.1 cm, and seed formation of Mammillaria goldii and M. theresae had internal body taking130 to 135 days to ripening seeds, however, M. pseudopectinata had externals taking just 26 days to ripening seeds. The mean seeded pod from April to June of M. goldii was 50.0% and the number of seeds in a pod was 25.3. In M. theresae, the mean seeded pod from April to June was 57.3% and the number of seeds in a pod was 35.0. However, the highest rate of seeded pod on May of M. pseudopectinata was 75.0% and the number of seed in a pod was 66.0. The sib crossing was much more ripening seed fertilization than that of self cross ing in the Mammillaria. The self crossing was formed from 12.0% to 20.0% of seeded pod, from 12.0 to 16.0 number of seed in a pod. However, sib crossing obtained 58.0% of seeded pod, 30 seeds in a pod of M. goldii, 65.0% seeded pods and 40 seeds in a pod of M. theresae, and 75.0% seeded pod, 68 seeds in a pod of M. pseudopectinala as the highest of them.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.291-296
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• 2009

This experiment was conducted to investigate the propagation system by seed to check growth, flowering, and making seed of four-year-old seedling cactus of potted Astrophytum asterias 'Nudum', A. asterias 'Super Kabuto', and A. myriostigma 'Nudum'. Plant height and width of Astrophytum asterias 'Nudum' and A. asterias 'Super Kabuto' was 5.3 to 5.5 cm, and 2.5 cm. However, plant height and width of A. myriostigma 'Nudum' was 7.6 cm and 5.6 cm as divided into 8 sections of body. In A. asterias 'Nudum', the mean seeded pod from April to September was 37.0% and the number of seeds in a pod was 43.0. In A. asterias 'Super Kabuto', the mean seeded pod from April to September was 22.5% and the number of seeds in a pod was 26.2. However, the mean seeded pod of A. myriostigma 'Nudum' from April to September was 32.3% and the number of seeds in a pod was 57.4. Especially, the seeded pod and the number of seeds in a pod of A. asterias 'Nudum' in July was the highest, 72.0% and 66.0. The sib crossing obtained much more seeded pods and the number of seeds per pod than that of selfing in the Astrophytum. The self crossing was formed from 9.0 to 20.0% of seeded pod, and 12.0 to 16.0 seeds in a pod. However, sib crossing was formed 60.0% seeded pod and 68.0 seeds in a pod on July of A. myriostigma 'Nudum'.

• The Korean Journal of Malacology
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• v.26 no.3
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• pp.217-225
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• 2010

The total oil proportion of bay scallop by areas during the growing period was the highest (2.8%) at Tongyong in August, then it decreased to 1.88% in September and 0.62% in October, and it was the lowest (0.22%) in November. The total oil proportion of phytoplankton by areas was the highest at Tongyong, where it was decreased from 5.02% in August and 3.29% in September to 2.48% in October and 1.66% in November. For the composition of fatty acid of bay scallop by areas and seasons during the growing period, the major composition was 16:0 and 18:0 as saturated fatty acid, and 16:1n-7, 18:1n-7, 20:1n-9, ARA (20:4n-6), EPA (20:5n-3), DHA (22:6n-3) as monoenic acid. TMTD (4,8,12-trimethyltridecanoic acid) was detected in a little amount as special fatty acid. For the composition of fatty acid of prey by areas during the growing period of bay scallop from August to November 1998, n-3HUFA, Omega-3 highly unsaturated fatty acid, in August was 47.11% at Namhae in slowest growth, while it was distinctively low with 34.26% at Tongyong and 14.06% at Nammeon.

• Korean Journal of Plant Resources
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• v.27 no.2
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• pp.194-201
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• 2014

This study was carried out to investigate the characteristics and infection effects of AMF with soil samples collected at some sites in Gyeonggi province, Korea. AMF spore and characteristics of infection structure in upland plant root were observed as wet sieving and staining method, growth of Capsicicum annum was compared between treatment and non treatment. AMF spores isolated from each soil sample were ellipse or circle type and the colors were soft yellow and white. The colonization rate of AMF with soil infection of Zea mays roots was 13.3~83.3%, the rate of infected soil collected from Z. mays was higher on average. When compared to the growth of C. Annum with control after infection on C. annum, it wasn't showing many differences in fresh weight, dry weight and height, but the yield of fruit of C. annuum showed double than the control group.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.312-321
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• 2018

Angelica tenuissima, also known as Ligusticum tenuissimum, is classified as a food-related plant and has been used as traditional medicines treating headache and anemia in Asia. However, its anti-melanogenic effect has not been reported in detail. When the extract of Angelica tenuissima (ATE) was prepared by the extraction with 70% EtOH at $80^{\circ}C$ (final yield = 22%), the contents of decursin and Z-ligustilide in ATE were determined 0.06% and 8.43%, respectively. Total flavonoid and phenolic content in mg ATE were $5.52{\pm}0.07{\mu}g$ quercetin equivalents and $237.27{\pm}13.24{\mu}g$ gallic acid equivalents, respectively. Antioxidant capacity of ATE determined by DPPH and ABTS assay was increased with a dose dependent manner up to $1000{\mu}g/m{\ell}$. The amount of melanin synthesis followed by ${\alpha}-melanocyte$ stimulating hormone on B16F10 cells were significantly reduced in the presence of ATE (250 to $1000{\mu}g/m{\ell}$, p<0.05). ATE (125 to $1000{\mu}g/m{\ell}$, p<0.05) suppressed the tyrosinase activity but did not show any significant effect on ${\alpha}-glucosidase$ activity at the same condition. Taken together, ATE possesses tyrosinase inhibitory potential with significant antioxidant capacities. These effects of ATE might be involved in suppression of melanin synthesis, at least, in B16F10 cells. The anti-melanogenic potential of ATE will provide an insight into developing a new skin whitening product.

• Journal of Life Science
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• v.31 no.9
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• pp.862-872
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• 2021

Recent studies on synthesis of metallic nanomaterials such as silver (Ag), gold (Au), platinum (Pt), cerium (Ce), zinc (Zn), and copper (Cu) nanoparticles (NPs) using plants and microbes are attracted researchers for their wide range of applications in the field of biomedical sciences. The plant contains abundant of bioactive contents such as flavonoids, alkaloids, saponins, steroids tannins and nutritionals components. Similarly, microbes produce bioactive metabolites, proteins and secretes valuable chemicals such as color pigments, antibiotics, and acids. Recently reported, biogenic synthesis of NPs in non-hazardous way and are promising candidates for biomedical applications such as antibacterial, antifungal, anti-cell proliferative and anti-plasmodia activity. All those activities are dose dependent, along with their shape and size also matters on potential of NPs. Microbes and plants are great source of metabolites, those useful in biomedical field, such metabolites or chemicals involved in synthesis of NPs in an ecofriendly way. NPs synthesized using microbes or plant materials are reveals more non-toxic, facile, and cost-effective compare to chemically synthesized NPs. In present review we are focusing on NPs synthesis using biological agents such as microbes (bacteria, fungi and algae) and plant, characterization using different techniques and their antibacterial applications on pathogenic Gram-positive and Gram-negative organisms.