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http://dx.doi.org/10.7847/jfp.2018.31.1.009

Monitoring of nervous necrosis virus in fertilized eggs of walleye pollock (Gadus chalcogrammus)  

Nam, U-Hwa (Department of Marine Bioscience, Gangneung-Wonju National University)
Lee, Jong-Hyuk (Department of Marine Bioscience, Gangneung-Wonju National University)
Kim, Mi-Ri (Department of Marine Bioscience, Gangneung-Wonju National University)
Jang, Su-Rim (Department of Marine Bioscience, Gangneung-Wonju National University)
Yoon, Do-Hyun (Department of Marine Bioscience, Gangneung-Wonju National University)
Seo, Joo-Young (Gangwon Cold Sea Fisheries Resources Center)
Kwon, O-Nam (GABI Co. Ltd.)
Kim, Jeong-Ho (Department of Marine Bioscience, Gangneung-Wonju National University)
Publication Information
Journal of fish pathology / v.31, no.1, 2018 , pp. 9-13 More about this Journal
Abstract
We previously monitored nervous necrosis virus (NNV) in brain samples of artificially produced walleye Pollock (Gadus chalcogrammus) seedlings, with a low prevalence (1.8%, 1/55) but no clinical symptoms. Given that this virus is considered one of the most serious viral threats for almost all marine aquaculture fish species and characterized by both vertical and horizontal transmission, it would be interesting to monitor NNV in the fertilized eggs as well. We collected fertilized walleye pollock eggs from the farms located in Goseong during January to March, 2017. Approximately 50 mg of eggs were periodically taken from 4 each different batches, and 37 different pooled sample sets in total were made during sampling period. RNA was extracted from the eggs by using Trizol and cDNA was synthesized for RT-PCR for detecting NNV. Primers and PCR conditions are the same as previously described. As a result, NNV was not detected from any of the sample sets by one step PCR (0%, 0/37), suggesting NNV may not be a threat in walleye pollock aquaculture in Korea at present time. However, continuous monitoring for NNV should be conducted because introducing a new species into aquaculture industry involves potentials of disease outbreak and NNV is already known to cause outbreaks in gadoid fishes.
Keywords
walleye pollock; Gadus chalcogrammus; nervous necrosis virus; NNV;
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Times Cited By KSCI : 3  (Citation Analysis)
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