• Journal of Advanced Marine Engineering and Technology
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• v.39 no.9
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• pp.923-928
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• 2015

The FDS is one of the most used programs for fire analysis and needs an optimal grid selection for an accurate analysis. This study selected various grid aspect ratios (ARs) for selection of optimal grid and analyzed them with FDS v 6.1.2. A calculation time of 10 min. was used, which is enough to obtain the time average value of temperature changes. Temperature, visibility, and the time average value of mass balance are obtained from 200-600 s, which is a period of maintaining quasi-steady state. Two polyurethane fires of 1 [MW] and 2 [MW] in two enclosures of $10{\times}10{\times}3[m^3]$ and $20{\times}20{\times}3[m^3]$ were considered. Time variations of heat release rates, temperature, visibility, and mass balance were compared for ARs from 1-6. The heat release rates were accurate for all aspect ratios regardless of fire and enclosure sizes. The quasi-steady state temperature and visibility were well predicted for $AR{\leq}5$. Temperature drop and skewness of mass conservation, however, increased with increasing aspect ratio. Therefore, careful investigation of the grid size is recommended in performance-based design when $AR{\geq}3$, where temperature and visibility in early stage of a fire are important parameters. For accurate simulations of enclosure fires, grid sizes of 0.1~0.2 [m] and smaller in the vertical direction and $AR{\leq}2$ are recommended.

• Journal of Advanced Marine Engineering and Technology
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• v.39 no.7
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• pp.722-728
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• 2015

The demand for shipboard energy management is expected to gradually increase based on ship energy efficiency management plans (SEEMPs), which have been in use since January 1, 2013. Therefore, the fuel consumption of the main engine, which occupies the greatest portion of the energy used, along with elements related to the engine power, should be strictly monitored. There are many different methods for indicating the engine power. However, this study performed an experiment to monitor the status of a ship's engine power in real time using an encoder and a proximate switch, which are economical to purchase and easy to install. In the experiment, the angular velocity during one cycle of a two-stroke low-speed engine was measured, and the measured data were converted to the torque fluctuation. The angular velocity during an abnormal firing condition in the cylinder was also measured, and the torque fluctuation as a result of a misfire was considered. The results were compared with sea trial data to determine the reliability. In this study, the status of the engine power was determined using the torque fluctuation of the main engine in an operating ship.

• Journal of Advanced Marine Engineering and Technology
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• v.38 no.3
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• pp.342-348
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• 2014

Onshore great fires can normally be extinguished by firefighters using special firefighting equipment and its suitable method. However, offshore fires on the ships are to be extinguished by the crew without any supports from the onshore. Also, crews working on board are exposed to high risk of emergency evacuation due to the complicated structure arrangement of the ships and different accident types such as fire and ship collisions. As most of damage and loss of life in fire are associated with suffocation, shortening of evacuation time is an important factor to improve a survival rate. In this study, visibility in the accommodation area is analyzed by using the temperature and smoke flow which are obtained by the Fire Dynamic Simulator(FDS) as a Three-Dimensional Fire Analysis program to understand the survival rate of the crew upon the fire. The fire doors for most of ships are designed to close automatically when the fire alarm is activated. These automatic closing of the fire doors is a very effective system to delay the spread of flame and smoke flow for the unmanned spaces of the fire protected area. However, if the crew cannot escape within the estimated time, the crew inside the fire protected area will be damaged a lot. In this paper, the comparative analysis between the evacuations by using the fire door from the fire protected area and the suggested fire shielding structure in this study is carried out by the smoke flow rate and the temperature rise rate.

• Journal of Navigation and Port Research
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• v.26 no.1
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• pp.112-119
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• 2002

In accordance with the development plans of Jeju for international free city, Jeju new harbor will be constructed cosidering supporting Jeju city as an international tour city, safe arrival and departure at the terminal of 80.000G/T cruise ship. In this study, we used a full-mission ship handling simulator adopting 80.000G/T cruise ship manoeuvered at the planned Juju international cruise terminal. Five masters who have had a long experience of shop maneuvering ware called to carry out the simulations of which No. 1 , Np. 2, and No.4 scenario of simulation were tried twice, completed the total of 10 times and No.3 scenario of simulation once, reached at the total of 5 time. The marine traffic safety was assessed in terms of 1) the closest point of approach(CPA) to breakwater and quay in the vicinty and the probability of crossing fairway limit, 2)subjective evaluation such as the mental burden and the maneuvering disfficulty of shiphanders and 3) the opinions of shiphandlers. From th result of this simulation, we have a conclusion that the 80.000G/T cruise ship can be maneuvered safely at the planned terminal.

• Journal of Advanced Marine Engineering and Technology
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• v.38 no.6
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• pp.658-665
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• 2014

Natural gas for marine diesel engine is considered as an important and clean source of energy because of simultaneously reducing the emission of NOx, SOx and GHG. Especially with a appearance of shale gas, the using of natural gas has been investigated aggressively and expected to expand rapidly. By the reports, gas engine and diesel engine were both in a similar performance in the power aspect, and the SFOC of gas engine was shown a little better than that of diesel engine. But the characteristics of exhaust gas emission were different according to various combustion technologies. And with lean burn technology, the emission of NOx could be reduced to 85% lower than that of diesel engine. In this paper, it was described that a simulation program has been developed to predict NOx emission. The developed program is adopted two-zone model and Wiebe function for combustion in cylinder. The effects of premixed and diffusive combustion could be simulated by using the excess air ratio as input data. And it was confirmed that the results of simulation were agreed with the general trends of exhaust gas emission according to various combustion conditions such as lean burn, premixed and diffusive combustion.

• Journal of Wetlands Research
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• v.6 no.1
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• pp.149-166
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• 2004

Water quality and ecological environment in the Han River estuary was analyzed using the longterm water quality monitoring data from National Fisheries Research and Development Institute (NFRDI) and the existing data collected in this area. Based on the salinity distribution and changes of current direction in the lower Han River and its estuary, boundaries of the estuary were identified and also, distribution patterns of the phyto- and zooplankton, benthos, ichthyoplankton and fish were discussed related with the salinity changes in the macrotidal subestuary of Han River. Seasonal and spatial distribution of salinity suggested that the direct impact of freshwater be limited to the Incheon North Harbour all the year round and even extended to the southern area of Gyunggi Bay near Palmi island during limited time, usually in summer. Upper limit of salt water intrusion through the Han River is likely to be Singok underwater dam located Gimpo, Gyunggi Province, and normally limited to much lower part of the river, Jeonryuri, Gimpo. Biological boundaries of the Han River estuary exceeded the physical boundaries based on the salinity distribution. Many estuarine species in plankton and fish were found at the totally freshwater or saltwater depending on the seasons and tidal cycles. Some estuarine ichthyoplanktons showed extremely limited distributions in the estuary whereas adult fish revealed wide ranges of salinity adaptation. Critical environmental issues in the Han River estuary and its drainage basin are likely to be 1) pressure on development-promoted district for new town in the drainage area of the estuary, 2) reduction of tidal flat by reclamation, 3) pollutant input through river from municipal sewages and industrial wastes, and 4) ecological barrier between river and terrestrial systems by the military wire fence and riverside road.

• The Korean Journal of Malacology
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• v.19 no.2
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• pp.125-132
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• 2003

Age and growth of the the Jedo venus clam, Protothaca jedoensis were investigated using samples from the subtidal zone of Boryeong, Chungcheongnam- do, Korea from January to December, 1999. The relationship between shell height and ring radius in each group was expressed as a regression line. Therefore, it could be recognized that there is a correspondence in the formation of each ring. Based on the monthly variation of the marginal index of the shell, it was suggested that the annual ring mark formation occurred during the period of February to March once a year. The relationship between shell height (SH) and total weight (TW) was represented by the non-linear equation: TW = 0.0007 SH$^{2.8919}$, and also in the relationship between shell length (SL) and shell height (SH), shell height (SH) and shell width (SW) were represented by the linear equations: SL=1.1067SH+1.778, SW=0.6758SH-0.9824. Growth curves for shell height and total weight fitted to the von Bertalanffy equation were expressed as: SH$_t$ = 81.546(1-e$^{-0.176(t+0.381)}$), TW$_t$ = 227.65(1-e$^{-0.176(t+0.381)})^{2.8919}$.

• Journal of Microbiology and Biotechnology
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• v.22 no.4
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• pp.555-562
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• 2012

PCR was performed to analyze the ${\beta}$-lactamase genes carried by ampicillin-resistant Vibrio spp. strains isolated from marine environments in Korea between 2006 and 2009. All 36 strains tested showed negative results in PCR with the primers designed from the nucleotide sequences of various known ${\beta}$-lactamase genes. This prompted us to screen new ${\beta}$-lactamase genes. A novel ${\beta}$-lactamase gene was cloned from Vibrio alginolyticus KV3 isolated from the aquaculture water of Geoje Island of Korea. The determined nucleotide sequence (VAK-3 ${\beta}$-lactamase) revealed an open reading frame (ORF) of 852 bp, encoding a protein of 283 amino acids (aa), which displayed low homology to any other ${\beta}$-lactamase genes reported in public databases. The deduced 283 aa sequence of VAK-3, consisting of a 19 aa signal peptide and a 264 aa mature protein, contained highly conserved peptide segments specific to class A ${\beta}$-lactamases including the specific amino acid residues STFK (62-65), SDN (122-124), E (158), and RTG (226-228). Results from PCR performed with primers specific to the VAK-3 ${\beta}$-lactamase gene identified 3 of the 36 isolated strains as V. alginolyticus, Vibrio cholerae, and Photobacterium damselae subsp. damselae, indicating the utilization of various ${\beta}$-lactamase genes including unidentified ones in ampicillin-resistant Vibrio spp. strains from the marine environment. In a mating experiment, none of the isolates transfered the VAK-3 ${\beta}$-lactamase gene to the Escherichia coli recipient. This lack of mobility, and the presence of a chromosomal acyl-CoA flanking sequence upstream of the VAK-3 ${\beta}$-lactamase gene, led to the assumption that the location of this new ${\beta}$-lactamase gene was in the chromosome, rather than the mobile plasmid. Antibiotic susceptibility of VAK-3 ${\beta}$-lactamase was indicated by elevated levels of resistance to penicillins, but not to cephalosporins in the wild type and E. coli harboring recombinant plasmid pKV-3, compared with those of the host strain alone. Phylogenetic analysis showed that VAK-3 ${\beta}$-lactamase is a new and separate member of class A ${\beta}$-lactamases.

• Microbiology and Biotechnology Letters
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• v.41 no.1
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• pp.112-118
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• 2013

This study was performed to investigate the possible use of Eisenia bicyclis (EB) ethanol extract to inhibit activity against lipase. In tests, the lipase inhibitory activity of EB ethanol extract was noted as being 43, 27, and 24% at concentrations of 5, 2.5, and 1 mg/ml, respectively. Isolation was carried out by liquid and liquid extraction, silica-gel column chromatography, and HPLC. The results showed that the lipase inhibitory activity of the ethyl acetate (EA) fraction from EB ethanol extract exhibited the strongest lipase inhibitory activity with an $IC_{50}$ value of 1.31 mg/ml. The EA fraction was separated using silica-gel column chromatography and we obtained 22 sub-fractions. Amongst them, the EA1 fraction showed the highest lipase inhibitory activity with an $IC_{50}$ value of 0.54 mg/ml. Eight peaks were obtained from the EA1 fraction by HPLC. Fraction 5 also showed a strong lipase inhibitory activity with an $IC_{50}$ value of 0.37 mg/ml. The fraction 5 was identified as dieckol and the inhibition pattern analyzed from Lineweaver-Burk plots revealed a non-competitive inhibitor. These results suggest that EB has potential as a natural anti-obesity agent.

• Microbiology and Biotechnology Letters
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• v.41 no.3
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• pp.372-378
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• 2013

An alginate-degrading bacterium, identified as Shewanella oneidensis PKA 1008 by 16S ribosomal RNA sequence analysis, was isolated from the green alga Ulva pertusa. Optimal conditions for the alginate-degrading ability of its crude enzyme were then determined. The optimal culture conditions for the growth of S. oneidensis PKA 1008 were pH 9, 2% NaCl, $30^{\circ}C$, and 24 hours incubation time. The crude enzyme produced by S. oneidensis PKA 1008 showed the highest alginate-degrading activity at pH 9, $30^{\circ}C$ and produced 1.001 g of reducing sugar per liter in 3.5% (w/v) sodium alginate for 1 hour.