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http://dx.doi.org/10.4014/kjmb.1210.10003

Lipase Inhibitory Mode of Dieckol Isolated from Eisenia bicyclis Ethanol Extract  

Jung, Seul-A (Department of Food Science and Technology / Institute of Food Science, Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences, Pukyong National University)
Kim, Dong-Hyun (Department of Food Science and Technology / Institute of Food Science, Pukyong National University)
Cho, Ji-Young (Department of marine biotechnology, Soonchunhyang University)
Kim, Tae-Wan (Department of Food Science and Biotechnology, Andong National University)
Ahn, Dong-Hyun (Department of Food Science and Technology / Institute of Food Science, Pukyong National University)
Publication Information
Microbiology and Biotechnology Letters / v.41, no.1, 2013 , pp. 112-118 More about this Journal
Abstract
This study was performed to investigate the possible use of Eisenia bicyclis (EB) ethanol extract to inhibit activity against lipase. In tests, the lipase inhibitory activity of EB ethanol extract was noted as being 43, 27, and 24% at concentrations of 5, 2.5, and 1 mg/ml, respectively. Isolation was carried out by liquid and liquid extraction, silica-gel column chromatography, and HPLC. The results showed that the lipase inhibitory activity of the ethyl acetate (EA) fraction from EB ethanol extract exhibited the strongest lipase inhibitory activity with an $IC_{50}$ value of 1.31 mg/ml. The EA fraction was separated using silica-gel column chromatography and we obtained 22 sub-fractions. Amongst them, the EA1 fraction showed the highest lipase inhibitory activity with an $IC_{50}$ value of 0.54 mg/ml. Eight peaks were obtained from the EA1 fraction by HPLC. Fraction 5 also showed a strong lipase inhibitory activity with an $IC_{50}$ value of 0.37 mg/ml. The fraction 5 was identified as dieckol and the inhibition pattern analyzed from Lineweaver-Burk plots revealed a non-competitive inhibitor. These results suggest that EB has potential as a natural anti-obesity agent.
Keywords
Eisenia bicyclis; lipase inhibotory; dieckol; anti-obesity agent;
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1 Prpkins, B. M. and C. M. Doak. 1998. The obesity prevalence is a worldwide phenomenon. Nutr. Rev. 56: 104-114.
2 Sugiura, Y., K. Matsuda, Y. Yamada, M. Nishikawa, K. Shioya, H. Katsuzaki, K. lmai, and H. Amano. 2006. Isolation of a new anti-allergic phlorotannin, phlorofucofuroeckol-B, from and edible brown alge, Eisenia arborea. Biosci. Biotech. Biochem. 70: 2807-2811.   DOI   ScienceOn
3 Westerterp-Plantenga, M. S., A. Smeets, and M. P. G. Lejeune. 2005. Sensory and gastrointestinal satiety effects of capsaicin on food intake. Int. J. Obesity. 29: 682-688.   DOI
4 Witvrouw, M. and E. De Clercq. 1997. Sulfated polysaccharides extracted from sea algae as potential antiviral drugs. Gen. Pharmacol. 29: 497-511.   DOI   ScienceOn
5 Ahn, M. J., K. D. Yoon, S. Y. Min, J. S. Lee, J. H. Kim, T. G.Kim, S. H. Kim, N. K. Kim, H. Huh, and J. W. Kim. 2004. Inhibition of HIV-1 reverse transcriptase and protease by phlorotannins from the brown alga Ecklonia cava. Biol. Pharm. Bull. 27: 544-547.   DOI   ScienceOn
6 Barwell, C. J., G. Blunden, and P. D. Manandhar. 1989. Isolation and characterization of brown algal polyphenols as inhibitors of α-amylase, lipase and trypsin. J. Appl. Phycol. 1: 319-323.   DOI   ScienceOn
7 Birari, R. B. and K. K. Bhutani. 2007. Pancreactic lipase inhibitors from natural sources: unexplored potential. Drug Discov. Today 12: 879-889.   DOI   ScienceOn
8 Bitou, N., M. Ninomiya, T. Tsujita, and H. Okuda. 1999. Screening of inhibitors from marine algae. Lipids 34: 441-445.   DOI   ScienceOn
9 Bray, G. A. and B. M. Popkin. 1998. Dietary fat intake dose affect obesity. Am. J. Clin. Nutr. 68: 1157-1173.
10 Cahyana, A. H., Y. Shuto, and Y. Kinoshita. 1992. Pyropheophytin a as an antioxidative substance from the marine alga, Arame (Eisenia bicyclis). Biosci. Biotech. Bioch. 56: 1533- 1535.   DOI
11 Drent, M. L., I. Larsson, T. William-Olsson, F. Quaade, F. Czubayko, K. Von Bergmann, W. Strobel, L. Sjotro, and E. A. Van der Veen. 1995. Orlistat (RO 18-0647), a lipase inhibitor, in the treatment of human obesity: a multiple dose study. Int. J. Obesity. 19: 221-226.
12 Chu, M. A. and B. H. Choe. 2010. Obesity and metabolic syndrome among children and adolescents in Korea. J. Korean Med. Assoc. 53: 142-152.   DOI
13 Cleland, W. W. 1967. Enzyme Kinetics. Annu. Rev. Biochem. 36: 77-112.   DOI   ScienceOn
14 Deavile, E. R., R. J. Green, I. Muller-Harvey, I. Willoughby, and R. A. Frazier. 2007. Hydrolyzable tannin structures influence relative globular and random coil protein binding strengths. J. Agr. Food Chem. 55: 4554-4561.   DOI   ScienceOn
15 Eom, S. H., M. S. Lee, E. W. Lee, Y. M. Kim, and T. H. Kim. 2012. Pancreatic lipase inhibitory activity of phlorotannins isolated from Eisenia bicyclis. Phytother. Res. DOI: 10.1002/ptr. 4696.
16 Gibbons, G. F. and D. Wiggins. 1995. Intracellular triacylglycerol lipase: Its role in the assembly of hepatic very low density lipoprotein (VLDL). Adv. Enzyme Regul. 35: 179-198.   DOI   ScienceOn
17 Hadvay, P., H. Lengsfeld, and H. Wolter. 1988. Inhibition of pancreatic lipase in vitro by covalent inhibitor tetrahydrolipstatin. Biochem. J. 256: 357-361.
18 Joo, D. S., J. K. Lee, Y. S. Choi, S.Y. Cho, Y. K. Je, and J. W. Choi. 2003. Effect of sea tangle oligosaccharide drink on serum and hepatic lipids in rats fed a hyperlipidemic diet. J. Korean Soc. Food Sci. Nutr. 32: 1364-1369.   DOI
19 Kang, K., Y. Park, H. J. Hwang, S. H. Kim, J. G. Lee, and H. C. Shin. 2003. Antioxidative properties of brown algae polyphenolics and their perspectives as chemopreventive agents against vascular risk factors. Arch. Pharm. Res. 26: 286-293.   DOI
20 Kang, M. C., Y. J. Lee, R. K. Ko, H. B. Kim, S. H. Hong, and G. O. Kim. 2008. Melanin inhibitory effect and anti-inflammatory effects of Dietyota coriacea extracts derived from adjacent sea of the Jeju island. Korean J. Biotechnol. Bioeng. 23: 311-316.
21 Kang, S. M., S. J. Heo, K. N. Kim, S. H. Lee, H. M. Yang, A. D. Kim, and Y. J. Jeon. 2012. Molecular docking studies of a phlorotannin, dieckol isolated from Ecklonia cava with tyrosinase inhibitory activity. Bioorgan. Med. Chem. 20: 311-316.   DOI   ScienceOn
22 Kim, D. H., E. H. Lee, J. C. Hwang, J. H. Jeung, D. H. Kim, J. Y. Cheong, S. W. Cho, and Y. B. Kim. 2002. A case of acute cholestatic hepatitis associated with orlistat. Korean J. Hepatol. 8: 317-320.
23 Kim, J. A., J. M. Lee, D. B. Shin, and N. H. Lee. 2004. The antioxidant activity and tyrosinase inhibitory of phlorotannin in Ecklonia cava. Food Sci. Biotechnol. 13: 476-480.
24 Kim, J. H., H. J. Kim, C. Y. Kim, H. Y. Jung, Y. O. Kim, J. Y. Ju, and C. S. Shin. 2007. Development of lipase inhibitors from various derivatives of monascus pigment produced by Monascus fermentation. Food Chem. 101: 357-364.   DOI   ScienceOn
25 Kim, K. B. W. R., J. Y. Jung, J. Y. Cho, and D. H. Ahn. 2012. Lipase inhibitory activity of ethyl acetate fraction from Ecklonia cava extracts. Biotechnol. Bioprocess Eng. 17: 739-745.   DOI   ScienceOn
26 Kim, K. Y., K. A. Nama, H. Kurihara, and S. M. Kim. 2008. Potent $\alpha$-glucosidase inhibitors purified from the red alga Grateloupia elliptica. Phytochemistry 69: 2820-2825.   DOI   ScienceOn
27 Kim, S. A., J. Kim, M. K. Woo, C. S. Kwak, and M. S. Lee. 2005. Antimutagenic and cytotoxic effects of ethanol extracts from five kinds of seaweeds. J. Korean Soc. Food Sci. Nutr. 34: 451-459.
28 Kong, C. S., Y. R. Um, J. I. Lee, Y. A. Kim, J. S. Lee, and Y. W. Seo. 2008. Inhibition effects of extracts and its solvent fractions isolated from Limonium tetragonum on growth of human cancer cells. Korean J. Biotechnol. Bioeng. 23: 177-182.
29 Kim, Y. M., C. K. Han, S. J. Bang, and J. H. Park. 2006. Effect of laminaran from Eisenia bicyclis on serum lipids in rats fed high choelsterol diet. J. Korean Soc. Food Sci. Nutr. 36: 841-846.
30 Kobayashi, A., T. Osaka, Y. Namba, S. Inoue, T. H. Lee, and S. Kimura. 1998. Capsaicin activates heat loss and heat production simultaneously and independently in rats. Am. J. Physiol. 275: 92-98.
31 Lee, H. O., D. S. Kim, J. R. Do, and Y. S. Ko. 1999. Angiotensin- I converting enzyme inhibitory activity of algae. J. Korean Fish Soc. 32: 427-431.
32 Lee, S. H., Y. Li, F. Karadeniz, M. M. Kim, and S. K. Kim. 2009. $\alpha$-Glucosidase and α-amylase inhibitory activities of phloroglucinal derivatives from edible marine brown alge, Ecklonia cava. J. Sci. Food Agric. 89: 1552-1558.   DOI   ScienceOn
33 Mattes, R. D. and L. Bormann. 2000. Effects of hydroxycitric acid on appetitive variables. Physiol. Behav. 71: 87-94.   DOI   ScienceOn
34 Moon, H. E., M. N. Islam, B. R. Ahn, S. S. Chowdhury, H. S. Sohn, H. A. Jung, and J. S. Choi. 2011. Protein tyrosine phosphatase 1B and α-glucosidase inhibitory phlorotannins from edible brown algae, Ecklonia stolonifera and Eisenia bicyclis.Biosci. Biotech. Bioch. 75: 1472-1480.   DOI   ScienceOn
35 Nakamura, T., K. Nagayama, K. Uchida, and R. Tanaka. 1996. Antioxidant activity of phlorotannins isolated from the brown alge Eisenia bicylis. Fisheries Sci. 62: 923-926.
36 Pi-Sunyer, F. X. 1991. Health implications of obesity. Am. J. Clin. Nutr. 5: 15955-16035.