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http://dx.doi.org/10.4014/kjmb.1212.12002

Optimization of Conditions for the Production and Properties of Alginate-degrading Crude Enzyme from Shewanella oneidensis PKA 1008  

Sunwoo, Chan (Department of Food Science & Technology/Institute, Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences, Pukyong National University)
Kim, Dong-Hyun (Department of Food Science & Technology/Institute, Pukyong National University)
Jung, Seul-A (Department of Food Science & Technology/Institute, Pukyong National University)
Kim, Hyun-Jee (Department of Food Science & Technology/Institute, Pukyong National University)
Jeong, Da-Hyun (Department of Food Science & Technology/Institute, Pukyong National University)
Jung, Hee-Ye (Department of Food Science & Technology/Institute, Pukyong National University)
Kang, Bo-Kyeong (Department of Food Science & Technology/Institute, Pukyong National University)
Bark, Si-Woo (Department of Food Science & Technology/Institute, Pukyong National University)
Lim, Sung-Mee (Department of Food Science & Technology, Tongmyong University)
Hong, Yong-Ki (Department of Biotechnology, Pukyong National University)
Ahn, Dong-Hyun (Department of Food Science & Technology/Institute, Pukyong National University)
Publication Information
Microbiology and Biotechnology Letters / v.41, no.3, 2013 , pp. 372-378 More about this Journal
Abstract
An alginate-degrading bacterium, identified as Shewanella oneidensis PKA 1008 by 16S ribosomal RNA sequence analysis, was isolated from the green alga Ulva pertusa. Optimal conditions for the alginate-degrading ability of its crude enzyme were then determined. The optimal culture conditions for the growth of S. oneidensis PKA 1008 were pH 9, 2% NaCl, $30^{\circ}C$, and 24 hours incubation time. The crude enzyme produced by S. oneidensis PKA 1008 showed the highest alginate-degrading activity at pH 9, $30^{\circ}C$ and produced 1.001 g of reducing sugar per liter in 3.5% (w/v) sodium alginate for 1 hour.
Keywords
Alginate-degrading crude enzyme; Shewanella oneidensis PKA 1008;
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