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Effect of Feeding Frequency of Extruded Pellet on Growth and Body Composition of Juvenile Flounder, Paralichthys olivaceus During the Winter Season (겨울철에 부상사료 공급횟수가 넙치 치어의 성장과 체성분에 미치는 효과)

  • Kim Gun-Up;Jang Hyun-Seok;Seo Joo-Young;Lee Sang-Min
    • Journal of Aquaculture
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    • v.18 no.1
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    • pp.31-36
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    • 2005
  • A feeding trial of two feeding rate (satiation and $80\%$ satiation) $\times$ four feeding frequency (three meals a day, two meals a day, one meal a day and one meal every two days) factorial design with three replications was carried out to investigate the optimum feeding frequency and rate for growth of flounder during the winter season. Juvenile flounder averaging 45 g were fed a extruded pellet for 60 days. Weight gain was affected by both feeding frequency (P<0.001) and feeding rate (P<0.01). The best weight gain obtained from fish fed two meals a day with satiation was not different from that of fish fed three meals a day with satiation and $80\%$ satiation. Feed efficiency and protein efficiency ratio of fish fed the diet three times daily were the highest among groups, but not different to other groups of fish except for one meal every two days with satiation and $80\%$ satiation feeding. Daily feed intake was affected by both feeding frequency and feeding rate (P<0.001), these values of fish fed two and three meals a day with satiation were the highest among the groups. Moisture, crude protein, crude lipid and ash contents of fish were not affected by both feeding frequency and feeding rate (P>0.05). The results of this study indicate that the optimum feeding frequency for the growth of juvenile flounder grown from 45 g to 53 g is two meals a day with satiation feeding during the winter season.

Effects of the Mixing Ratio of the Different Substrates and the Concentration of Fertigation in Nutrient Solution on the Growth of Tomato Plug Seedlings (배지의 혼합비율과 관비 양액 농도가 토마토 플러그묘의 생장에 미치는 영향)

  • Kim, Hong-Gi;Cho, Ja-Yong;Yu, Sung-Oh;Yang, Seung-Yul;Kang, Jong-Gu;Heo, Buk-Gu
    • Journal of Bio-Environment Control
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    • v.16 no.2
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    • pp.108-114
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    • 2007
  • This study was conducted to clarify the effects of the different mixing ratios of substrate mixtures based on peat moss and the concentration of nutrient solution on the growth of tomato (Lycopersicon esculentum Mill.) seedlings. Substrates such as peat moss, rice hull, carbonized rice hull, decomposed sawdust, perlite and granular rock wool were mixed and used. The concentration of nutrient solution were adjusted to EC $0.5{\sim}1.5mS/cm$. The volumetric moisture contents became higher as peat moss mixed were much more. Total porosities in all substrate mixtures were over 80%, and pH in substrate mixtures became lower as the volume of peat moss mixed higher. Mixing ratios of substrates suitable for the production of tomato seedlings with the higher quality were peat moss:rice hull:carbonized rice hull:decomposed sawdust:perlite=25:10:25:20:20(v/v). The plant growth was not significant among the different substrate mixtures. However, plant growth such as plant height, leaf area, and total dry weight became significantly increased as EC increasing.

Morphology of Neoheterobothrium hirame parasitic in the oral cavity of olive flounder (Paralichthys olivaceus) and histopathology of diseased fish (넙치(Paralichthys olivaceus) 구강에 기생하는 Neoheterobothrium hirame의 형태 및 감염어의 병리조직)

  • Lee, Beom-Seok;Kim, Jeong-Ho;Kim, Taeho;Jung, Sung-Ju
    • Journal of fish pathology
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    • v.33 no.1
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    • pp.7-14
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    • 2020
  • A disease that manifested severe anemia of the gills occurred in the olive flounder (Paralichthys olivaceus), which was cultured for 5-6 months with a recirculation water system in the laboratory. Microscopic observations showed immature parasite in the gills and mature adults in the oral cavity. The matured parasite was 5.60-9.32 (7.42) mm in total length, with 4 pairs of clamps on the pedunculated haptor, which was separated from the body proper by a long isthmus. From the morphological observations of the larvae, it was identified as Neoheterobothirum hirame belonging to the monogenea. The average hematocrite of infected fish was 10.3 ± 2.8%, significantly lower than that of normal flounder 31.4 ± 4.2%. Histopathologically, fish infected with N. hirame exhibited reduced numbers of erythrocytes in blood vessels of the gill filament and lamellae, reduced red pulp area of the spleen, and hepatocyte atrophy. From the above results, the cause of severe anemia in olive flounder was identified as N. hirame infection. This study shows an example where the growth of pathogens can be a problem in the recirculation system due to low water exchange rate.

Application of Lignocellulosic and Macro-algae Hydrolysates for Culture of Chlorella saccharophila (Chlorella saccharophila 배양을 위한 목질계 및 해조류 바이오매스 가수분해물의 이용)

  • Kim, A-Ram;Kim, Hyo Seon;Park, Mi-Ra;Kim, Sung-Koo;Jeong, Gwi-Taek
    • Microbiology and Biotechnology Letters
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    • v.44 no.4
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    • pp.522-528
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    • 2016
  • In this study, we investigated the possibility of using hydrolysates of lignocellulosics (rapeseed straw, barley straw, rice straw) and marine macro-algae (Undaria pinnatifida, Laminaria japonica, Enteromorpha intestinalis, and Gracilaria verrucosa) to cultivate Chlorella saccharophila. The growth of C. saccharophila was inhibited by 7 hydrolysates without active carbon treatment. In contrast, hydrolysates treated with active carbon increased the cell growth and product (oil and chlorophyll) formation by C. saccharophila. The oil contents of C. saccharophila treated with each hydrolysate were $41.26{\pm}0.69%$ (glucose), $22.06{\pm}1.21%$ (rapeseed straw), $28.65{\pm}1.08%$ (barley straw), $31.15{\pm}0.76%$ (rice straw), $31.50{\pm}2.12%$ (U. pinnatifida), $31.49{\pm}4.53%$ (L. japonica), $29.63{\pm}3.93%$ (E. intestinalis), and $26.15{\pm}1.99%$ (G. verrucosa), respectively. Lignocellulosics and marine macro-algae may be useful resources for improving the mass cultivation of C. saccharophila.

Effects of Non-ionic Surfactants on Enzyme Distributions of Rumen Contents, Anaerobic Growth of Rumen Microbes, Rumen Fermentation Characteristics and Performances of Lactating Cows

  • Lee, S.S.;Ahn, B.H.;Kim, H.S.;Kim, C.H.;Cheng, K.-J.;Ha, J.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.1
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    • pp.104-115
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    • 2003
  • A series of experiments was carried out to determine the possibility for the non-ionic surfactant (NIS) as a feed additive for ruminant animals. The effect of the NIS on (1) the enzyme distribution in the rumen fluids of Hereford bulls, (2) the growth of pure culture of rumen bacteria and (3) rumen anaerobic fungi, (4) the ruminal fermentation characteristics of Korean native cattle (Hanwoo), and (5) the performances of Holstein dairy cows were investigated. When NIS was added to rumen fluid at the level of 0.05 and 0.1% (v/v), the total and specific activities of cell-free enzymes were significantly (p<0.01) increased, but those of cell-bound enzymes were slightly decreased, but not statistically significant. The growth rates of ruminal noncellulolytic species (Ruminobacter amylophilus, Megasphaera elsdenii, Prevotella ruminicola and Selenomonas ruminantium) were significantly (p<0.01) increased by the addition of NIS at both concentrations tested. However, the growth rate of ruminal cellulolytic bacteria (Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens and Butyrivibrio fibrisolvens) were slightly increased or not affected by the NIS. In general, NIS appears to effect Gram-negative bacteria more than Gram-positive bacteria; and non-cellulolytic bacteria more than cellulolytic bacteria. The growth rates of ruminal monocentric fungi (Neocallimastix patriciarum and Piromyces communis) and polycentric fungi (Orpinomyces joyonii and Anaeromyces mucronatus) were also significantly (p<0.01) increased by the addition of NIS at all concentrations tested. When NIS was administrated to the rumen of Hanwoo, Total VFA and ammonia-N concentrations, the microbial cell growth rate, CMCase and xylanase activities in the rumen increased with statistical difference (p<0.01), but NIS administration did not affect at the time of 0 and 9 h post-feeding. Addition of NIS to TMR resulted in increased TMR intake and increased milk production by Holstein cows and decreased body condition scores. The NEFA and corticoid concentrations in the blood were lowered by the addition of NIS. These results indicated that the addition of NIS may greatly stimulate the release of some kinds of enzymes from microbial cells, and stimulate the growth rates of a range of anaerobic ruminal microorganisms, and also stimulate the rumen fermentation characteristics and animal performances. Our data indicates potential uses of the NIS as a feed additive for ruminant animals.

Practical Problem-Focused Instructional Design and It's Perception Applying Universal Design (유니버설 디자인을 적용한 체험형 실천적 문제중심 수업 설계 및 수업 인식)

  • Lee, Gyeong-Suk;Jang, Sang-Ock
    • Journal of Korean Home Economics Education Association
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    • v.22 no.2
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    • pp.155-169
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    • 2010
  • This study aims to design practical problem-focused instruction by applying universal design of "Housing" unit of Technology Home Economics in the 10th grade and examine the students' perception on the lesson. The lesson is composed of the following procedures: thinking, problem recognition, problem understanding, information search & alternative search, consideration of the result, and practice plan. To solve problem such as 'What should one do for everyone's convenient and comfortable residential life' practically, we chose the major concern of 'understanding the universal design', 'analysis of cause for environmental change', 'creation of human-concerned residential culture'and performed alternative experience program for the old, the pregnant and the disabled. The students perceived teaching methods and contents positively with interest. They also understood the lesson easily by experiential activity and discussion thus enabling their active participation. It was also found out that the students felt considerable change of their consciousness of living environment of residence and the local community, thus it is concluded that the practical problem-focused instructional design method is very effective.

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A Study on Comparative Analysis of Food Characteristics of Sea Bream and Similar Species (도미와 유사어종의 식품학적 특성비교 분석에 관한 연구)

  • Chung, Ji-Yong;Jung, Young Mi
    • Culinary science and hospitality research
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    • v.23 no.2
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    • pp.159-168
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    • 2017
  • This study was conducted to compare the food quality of domesticated species. Consumers surveyed for safe food intake and proper culture of food distribution. The results of the comparison study are as follows. Muscle moisture content, protein content, and fat content. K, P, and C showed relatively high values in the muscle of the sea bream. Fe showed low contents. As a result of measuring heavy metal component, Cd was not detected in sea bream and mullet, but $0.01{\pm}0.00mg/kg$ was detected in red mine. Other heavy metals were below the reference value or were not detected. Electrophoresis results showed that the band appeared at in red minefish. In the case of sea bream and swordfish, no distinctive features of the band were shown. In the case of sea bream, there was little difference in food science between the similar fish species and the red sea bream fish, but price was different. An environment should be created for consumers to buy the right ingredients at the price they want. It is necessary to educate consumers about food ingredients immediately.

Effects of Fertilizers on Growth and Yield of Tree Peony Moutan cortex RADICIS (모단 재배시 비료 종류가 생육 및 수량에 미치는 영향)

  • 최인식
    • Korean Journal of Plant Resources
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    • v.9 no.3
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    • pp.261-267
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    • 1996
  • This experiment was carried out to investigate the effects of various fertilizers on the growth and yield of tree peony Moutan cortex RADICIS from 1990 to 1994. The paeonol content, a main medical ingredient of tree peony, was 0.5% in the control, and the contents of the chemical fertilizer(N-P-K) and the two-kind compound fertilizer treatments were $0.26{\sim}0.41%$, and those were lower than $0.40{\sim}0.45$ of the compost, the fowl dropping manure and the organic fertilizer treatments. The weights off dry root(radix) rind were increased by 42% in the fowl dropping manure treatment and by 49% in the compost treatment compared with 151kg/10a in the control. All things considered, compost, fowl dropping manure and organic fertilizer were more effective than chemical fertilizers for the culture of tree peony.

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Elicitation of Indole-3-ylmethyl Glucosinolate Biosynthesis in Turnip Culture Cells and Their Relationship with Plant Resistance to Botrytis cinerea (잿빛곰팡이병 추출물을 이용한 순무배양세포의 Indole-3-ylmethyl glucosinolate의 생합성유도와 병원성연구)

  • Kwon, Soon Tae;Zhang, Vivian
    • Korean Journal of Plant Resources
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    • v.30 no.5
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    • pp.542-548
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    • 2017
  • Two different races of Botryris cinerea were selected by the response of plant leaves to the pathogen infection. Based on lesion size of the pathogen on the leaves, turnip showed susceptible response to 'Grape-01' race, and resistant to 'Orange' race. Turnip leaves infected with resistant pathogen race, "Orange", showed significantly higher content of indole-3-ylmethyl glucosinolate (I3M) than those infected with susceptible race, 'Grape-01'. Contents of I3M in the leaves with resistant 'Orange' race was 2.5 times as high as that in uninfected leaves, whereas I3M in the leaves infected with susceptible 'Grape-01' race showed lower content than in untreated leaves. Growth of turnip suspension cells was significantly inhibited by the treatment of MeOH extract or water extract of 'Orange' race as compared with the treatment of susceptible race, 'Grape-01'. Treatment of MeOH or water extract from 'Orange' race to turnip suspension cells, strongly inhibited cell viability up to 22.7% or 16.5%, respectively. However, plant cells treated with MeOH or water extract from resistant race, 'Orange' showed higher I3M content than that from susceptible race, 'Grape-01'. These results suggest that accumulation and degradation of I3M glucosinolate in turnip cells closely related to the resistance and susceptibility of turnip cells to Botrytis cinerea.

Soil Resilience and Threat Factors Related to Agricultural Environment (농업환경 분야에서의 토양 리질리언스와 그 위협 요인)

  • Kim, Min-Suk;Min, Hyun-Gi;Hyun, Seung-Hun;Kim, Jeong-Gyu
    • Ecology and Resilient Infrastructure
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    • v.7 no.1
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    • pp.26-42
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    • 2020
  • Soils are the basis for plant rooting and ecosystem creation, the site of life for humankind, and require much time for their creation, so there will be no disagreement about the importance and necessity of soil conservation and management. Soil resilience is the ability of soils to maintain their original structure and function (resistance and recovery) from various kinds of disturbances, and is an indispensable field of study that prepares for a future with high uncertainty and unpredictability. Therefore, this study summarizes the concept and necessity of soil resilience, which is not yet widely known in Korea, and the contents of previous studies were reviewed. This study was carried out with the aim of contributing to lowering the threshold for entry into resilience research for domestic and foreign researchers who are new to soil resilience. In the first part of this study, we introduced resilience and soil resilience, and in the second part, we summarized the main causes of stress or disturbance that have been studied by many soil resilience researches. This makes it easy to find the references authors need. It is virtually impossible to find the same soil environment because there is no same area on the earth with all the same rock, climate, human activity, and culture, suggesting that each soil has its own uniqueness. Therefore, the researcher who wants to utilize the results of this study should take into consideration the specificity of the soil and the region to which the soil resilience is introduced, and modify it if necessary. In addition, efforts should be made to strengthen the network of soil resilience researchers to create a basis for sharing and actively utilizing the research results.