Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Morphology of Neoheterobothrium hirame parasitic in the oral cavity of olive flounder (Paralichthys olivaceus) and histopathology of diseased fish

넙치(Paralichthys olivaceus) 구강에 기생하는 Neoheterobothrium hirame의 형태 및 감염어의 병리조직

  • Lee, Beom-Seok (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Jeong-Ho (Department of Marine Bioscience, Gangneung-Wonju National University) ;
  • Kim, Taeho (Division of Culture Contents, Chonnam National University) ;
  • Jung, Sung-Ju (Department of Aqualife Medicine, Chonnam National University)
  • 이범석 (전남대학교 수산생명의학과) ;
  • 김정호 (강릉원주대학교 해양자원육성학과) ;
  • 김태호 (전남대학교 해양생산관리학과) ;
  • 정성주 (전남대학교 수산생명의학과)
  • Received : 2020.05.31
  • Accepted : 2020.06.15
  • Published : 2020.06.30
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Abstract

A disease that manifested severe anemia of the gills occurred in the olive flounder (Paralichthys olivaceus), which was cultured for 5-6 months with a recirculation water system in the laboratory. Microscopic observations showed immature parasite in the gills and mature adults in the oral cavity. The matured parasite was 5.60-9.32 (7.42) mm in total length, with 4 pairs of clamps on the pedunculated haptor, which was separated from the body proper by a long isthmus. From the morphological observations of the larvae, it was identified as Neoheterobothirum hirame belonging to the monogenea. The average hematocrite of infected fish was 10.3 ± 2.8%, significantly lower than that of normal flounder 31.4 ± 4.2%. Histopathologically, fish infected with N. hirame exhibited reduced numbers of erythrocytes in blood vessels of the gill filament and lamellae, reduced red pulp area of the spleen, and hepatocyte atrophy. From the above results, the cause of severe anemia in olive flounder was identified as N. hirame infection. This study shows an example where the growth of pathogens can be a problem in the recirculation system due to low water exchange rate.

실험실에서 순환여과시스템으로 5-6개월 사육하던 넙치 치어에서 아가미의 심한 빈혈을 나타내는 질병이 발생하였다. 현미경관찰에서 아가미에서는 유충이, 입천정에서는 성숙한 성충이 관찰되었다. 성충의 체장은 5.60-9.32 mm이며 방추형 몸의 길이는 3.83-6.32 mm, 부착기는 방추형 몸에서 부터 연결된 isthmus의 끝부분에 꽃자루 모양으로 존재하였고 부착기의 끝에는 8개의 파악기가 있었다. 충의 형태학적 관찰로부터 단생류에 속하는 Neoheterobothirum hirame로 동정하였다. 감염어의 적혈구용적는 평균 10.3±2.8%로 정상 넙치의 31.4±4.2%보다 현저히 낮았다. 병리조직학적으로 N. hirame에 감염 된 넙치는 아가미 새판과 새엽을 흐르는 혈관내의 적혈구가 감소하고 비장의 적수(red pulp) 영역이 감소되고 간세포는 위축이 관찰되었다. 이상의 결과로부터 넙치에 심한 빈혈을 일으킨 원인은 N. hirame인 것을 밝혔다. 이 연구는 순환여과식 사육시스템에서는 사육수의 교환이 적으므로 소독장치가 충분하지 못할 경우 병원체의 증식이 문제가 될 수 있는 예를 보이고 있다.

Keywords

References

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