• Elastomers and Composites
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• v.39 no.1
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• pp.23-35
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• 2004

In this work, effects of hyper-elastic rubber material properties on the indentation load-deflection curve and subindenter deformation are first examined via finite element (FE) analyses. An optimal data acquisition spot is selected, which features maximum strain energy density and negligible frictional effect. We then contrive two normalized functions, which map an indentation load vs. deflection curve into a strain energy density vs. first invariant curve. From the strain energy density vs. first invariant curve, we can extract the rubber material properties. This new spherical indentation approach produces the rubber material properties in a manner more effective than the common uniaxial tensile/compression tests. The indentation approach successfully measures the rubber material properties and the corresponding nominal stress-strain curve.

• Molecules and Cells
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• v.23 no.1
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• pp.108-114
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• 2007

The mitogen-activated protein kinase (MAPK) signaling cascade is critical for regulating plant defense systems against various kinds of pathogen and environmental stresses. One component of this cascade, the MAP kinase kinases (MAPKK), has not yet been shown to be induced in plants following biotic attacks, such as those by insects and fungi. We describe here a gene coding for a blast (Magnaporthe grisea)- and insect (Nilaparvata lugens)-responsive putative MAPK kinase, OmMKK1 (Oryza minuta MAPKK 1), which was identified in a library of O. minuta expressed sequence tags (ESTs). Two copies of OmMKK1 are present in the O. minuta genome. They encode a predicted protein with molecular mass 39 kDa and pI of 6.2. Transcript patterns following imbibition of plant hormones such as methyl jasmonic acid (MeJA), ethephone, salicylic acid (SA) and abscisic acid (ABA), as well as exposure to methyl viologen (MV), revealed that the expression of OmMKK1 is related to defense response signaling pathways. A comparative analysis of OmMKK1 and its O. sativa ortholog OsMKK1 showed that both were induced by stress-related hormones and biotic stresses, but that the kinetics of their responses differed despite their high amino acid sequence identity (96%).

• Journal of Microbiology
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• v.44 no.4
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• pp.375-382
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• 2006

From its initial colonization to causation of disease, Streptococcus pneumoniae has evolved strategies to cope with a number of stressful in vivo environmental conditions. In order to analyze a global view of this organism's response to heat shock, we established a 2-D electrophoresis proteome map of the S. pneumoniae D39 soluble proteins under in vitro culture conditions and performed the comparative proteome analysis to a 37 to $42^{\circ}C$ temperature up-shift in S. pneumoniae. When the temperature of an exponentially growing S. pneumoniae D39 culture was raised to $42^{\circ}C$, the expression level of 25 proteins showed changes when compared to the control. Among these 25 proteins, 12 were identified by MALDI-TOF and LC-coupled ESI MS/MS. The identified proteins were shown to be involved in the general stress response, energy metabolism, nucleotide biosynthesis pathways, and purine metabolism. These results provide clues for understanding the mechanism of adaptation to heat shock by S. pneumoniae and may facilitate the assessment of a possible role for these proteins in the physiology and pathogenesis of this pathogen.

• Journal of Life Science
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• v.19 no.7
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• pp.949-955
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• 2009

Mucosal epithelia sense external stress signals and transmit them to the intracellular cascade responses. Ribotoxic stress-producing chemicals such as deoxynivalenol (DON) or other trichothecene mycotoxins have been linked with gastrointestinal inflammatory diseases by Fusarium-contamination. The purpose of this study was to test the hypothesis that DON evokes the epithelial sentinel signals of RNA-dependent protein kinase (PKR) and early growth response gene 1 (EGR-1), which together contribute to the pro-inflammatory cytokine interleukin 8 (IL-8) in human intestinal epithelial cells. PKR suppression by the dominant negative PKR expression attenuated DON-stimulated interleukin-8 production. Moreover, 1L-8 transcriptional activation by DON was also reduced by PKR inhibition in the human intestinal epithelial cells. Treatment with the PKR inhibitor also suppressed EGR-1 promoter activity, mRNA and protein induction, although mitogen-activated protein (MAP) kinases such as extracellular signal-regulated protein kinases (ERK) 1/2, p38, c-Jun N-terminal Kinase (INK) were little affected or even enhanced in presence of a PKR inhibitor. These patterns were also compared in the EGR-1-suppressed cells, which showed much more suppressed production of 1L-8. All things taken into consideration, DON-activated sentinel signals of EGR-1 via PKR mediated interleukin-8 production in human intestinal epithelial cells, which provide insight into the possible general mechanism associated with mucosal inflammation as an intestinal toxic insult by ribotoxic trichothecene mycotoxins.

• Composites Research
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• v.31 no.4
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• pp.145-150
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• 2018

Various structural health monitoring (SHM) systems have been suggested for aerospace industry in order to increase its life-cycle and economic efficiency. In the case of aircraft structure madden with metal, a major concern was hot spots, such as notches, bolts holes, and where corrosion or stress concentration occurs due to moisture or salinity. However, with the increasing use of composites in the aerospace industry, further advanced SHM systems have been being required to be applied to composite structures, which have much complex damage mechanism. In this paper, a method of acoustic emission localization for composite structures using Q-switched laser and multiple Amplifier-integrated PZTs have been proposed. The presented technique aims at localization of the AE with an error in distance of less than 10 mm. Acoustic emission simulation and the localization attempt were conducted in the composite structure to validate the suggested method. Localization results, which are coordinates of detected regions, grid plots and color intensity map have been presented together to show reliability of the method.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2004.03a
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• pp.848-853
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• 2004

Regarding to the project SUAV (Smart Unmanned Aerial Vehicle) in KARI (Korea Aerospace Research Institute), several engine configurations has been evaluated. However it's not an easy task to collect all the necessary data of each engine for the analysis. Usually, some kind of modeling technique is required in order to determine the unknown data. In the present paper a qualitative method for reverse engineering is proposed, in order to identify some design patterns and relationships between parameters. The method can be used to estimate several parameters that usually are not provided by the manufacturer. The method consists of modeling an existing engine and through a simulation, compare its transient behavior with its operating envelope. In the simulation several parameters such as thermodynamics, performance, safety and mechanics concerning to the definition of operation-envelope, have been discussed qualitatively. With the model, all engine parameters can be estimated with acceptable accuracy, making possible the study of dependencies among different parameters such as power-turbine total inertia, TIT, take-off time and part load, in order to check if the engine transient performance is within the design criteria. For more realistic approach and more detailed design requirements, it will be necessary to enhance the compressor map first, and more realistic estimated values must be taken into account for intake-loss, bleed-air and auxiliary power extraction. The relative importance of these “unknown” parameters must be evaluated using sensitivity analysis in the future evaluation. Moreover, fluid dynamics, thermal analysis and stress analysis necessary for the resulting life assessment of en engine, will not be addressed here but in a future paper. With the methodology presented in the paper was possible to infer the relationships between operation-envelope and engine parameters.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.185-185
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• 2023

Agricultural water demand is considered as the major sector of water withdrawal due to irrigation. The majority part of the global agricultural field depends on various irrigation techniques. Therefore, a timely and sufficient supply of water is the most important requirement for agriculture. Irrigation is implemented in different ways in various land surface models, it can be modeled empirically based on observed irrigation rates or by calculating water supply and demand. Certain models can also calculate the irrigation demand as per the soil water deficit. In these implementations, irrigation is typically applied uniformly over the irrigated land regardless of crop types or irrigation techniques. Whereas, the latest version of Community Land Model (CLM) in the Community Terrestrial Systems Model (CTSM) uses a global distribution map of irrigation with 64 crop functional types (CFTs) to simulate the irrigation water demand. It can estimate irrigation water withdrawal from different sources and the amount or the areas irrigated with different irrigation techniques. Hence, we set up the model for the simulation period of 16 years from 2000 to 2015 to analyze the global irrigation demand at a spatial resolution of 1.9° × 2.5°. The simulated irrigation water demand is evaluated with the available observation data from FAO AQUASTAT database at the country scale. With the evaluated model, this study aims to suggest new sustainable scenarios for the ratios of irrigation water withdrawal, high depending on the withdrawal sources e.g. surface water and groundwater. With such scenarios, the CFT maps are considered as the determining factor for selecting the areas where the crop pattern can be altered for a sustainable irrigation water management depending on the available withdrawal sources. Overall, our study demonstrate that the scenarios for the future sustainable water resources management in terms of irrigation water withdrawal from the both the surface water and groundwater sources may overcome the excessive stress on exploiting the groundwater in major river basins globally.

• Journal of the Korean Geotechnical Society
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• v.34 no.8
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• pp.37-49
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• 2018

Since the observation of ground motions in South Korea, liquefaction manifestation was the first to be observed in Pohang earthquake in 2017 with $M_L$ 5.4. Because liquefaction causes ground settlement and lateral spread damaging in-ground or super structures, various researchers have been analyzing the Pohang liquefaction case history to better understand and predict liquefaction consequence and to prevent future disasters. In prior research at the 2018 EESK conference, a map of Liquefaction Potential Index (LPI), indicating the severity of liquefaction, in Pohang was created and compared with damage observations. The LPI correlated well with the observations, but the severity categorized by LPI range was significantly higher than the actual observations in most regions. The prior LPI map was created evaluating ground motions using the simplified approach. In this research, we perform the effective site response analyses with porewater pressure generation model for the detailed evaluation of liquefaction on the liquefied sites in Pohang. We found that the simplified approach for LPI evaluation can overestimate the severity.

• Korean Journal of Agricultural and Forest Meteorology
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• v.19 no.1
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• pp.10-18
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• 2017

Korean Red Pine (Pinus densiflora) has been protected and used as the most ecologically and socio-culturally important tree species in Korea. However, as dieback of Korean red pines has occurred in the protected area of the forest genetic resources. The aims of this study is to identify causes for dieback of pine tree by investigating topographical characteristics of pine tree dieback and its correlation to meteorological factors. We extracted the dead trees from the time series aerial images and analyzed geomorphological characteristics of dead tree concentration area. As a result, 1,956 dead pine trees were extracted in the study region of 2,600 ha. Dieback of pine trees was found mostly in the areas with high altitude, high solar radiation, low topographic wetness index, south and south-west slopes, ridgelines, and high wind exposure compared to other living pine forest area. These areas are classified as high temperature and high drought stress regions due to micro-climatic characteristics affected by topographic factors. As high temperature and drought stress are generally increasing with climate change, we can evaluated that a risk of pine tree dieback is also increasing. Based on these geomorphological characteristics, we developed a pine tree dieback risk map using Maximum Entropy Model (MaxEnt), and it can be useful for establishing Korean red pine protection and management strategies.

• Molecular & Cellular Toxicology
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• v.3 no.2
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• pp.98-106
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• 2007

Genotoxic stress triggers a variety of biological responses including the transcriptional activation of genes regulating DNA repair, cell survival and cell death. In this study, we investigated to examine gene expression profiles and genotoxic response in TK6 cells treated with DNA damaging agents MNNG (N-methyl-N'-nitrosoguanidine) and hydrogen peroxide $(H_2O_2)$. We extracted total RNA in three independent experiments and hybridized cRNA probes with oligo DNA chip (Applied Biosystems Human Genome Survey Microarray). We analyzed raw signal data with R program and AVADIS software and identified a number of deregulated genes with more than 1.5 log-scale fold change and statistical significancy. We indentified 14 genes including G protein alpha 12 showing deregulation by MNNG. The deregulated genes by MNNG represent the biological pathway regarding MAP kinase signaling pathway. Hydrogen peroxide altered 188 genes including sulfiredoxins. These results show that MNNG and $H_2O_2$ have both uniquely regulated genes that provide the potential to serve as biomarkers of exposure to DNA damaging agents.