Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Gene Expression Profiling of Genotoxicity Induced by MNNG in TK6 Cell

  • Suh, Soo-Kyung (Genetic Toxicology Team, Toxicological Research Department, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim, Tae-Gyun (Genetic Toxicology Team, Toxicological Research Department, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim, Hyun-Ju (Genetic Toxicology Team, Toxicological Research Department, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Koo, Ye-Mo (Genetic Toxicology Team, Toxicological Research Department, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Lee, Woo-Sun (Genetic Toxicology Team, Toxicological Research Department, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Jung, Ki-Kyung (Genetic Toxicology Team, Toxicological Research Department, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Jeong, Youn-Kyoung (Genetic Toxicology Team, Toxicological Research Department, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kang, Jin-Seok (Genetic Toxicology Team, Toxicological Research Department, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim, Joo-Hwan (Genetic Toxicology Team, Toxicological Research Department, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Lee, Eun-Mi (Genetic Toxicology Team, Toxicological Research Department, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Park, Sue-Nie (Genetic Toxicology Team, Toxicological Research Department, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim, Seung-Hee (Genetic Toxicology Team, Toxicological Research Department, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Jung, Hai-Kwan (Genetic Toxicology Team, Toxicological Research Department, National Institute of Toxicological Research, Korea Food and Drug Administration)
  • Published : 2007.06.30
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Abstract

Genotoxic stress triggers a variety of biological responses including the transcriptional activation of genes regulating DNA repair, cell survival and cell death. In this study, we investigated to examine gene expression profiles and genotoxic response in TK6 cells treated with DNA damaging agents MNNG (N-methyl-N'-nitrosoguanidine) and hydrogen peroxide $(H_2O_2)$. We extracted total RNA in three independent experiments and hybridized cRNA probes with oligo DNA chip (Applied Biosystems Human Genome Survey Microarray). We analyzed raw signal data with R program and AVADIS software and identified a number of deregulated genes with more than 1.5 log-scale fold change and statistical significancy. We indentified 14 genes including G protein alpha 12 showing deregulation by MNNG. The deregulated genes by MNNG represent the biological pathway regarding MAP kinase signaling pathway. Hydrogen peroxide altered 188 genes including sulfiredoxins. These results show that MNNG and $H_2O_2$ have both uniquely regulated genes that provide the potential to serve as biomarkers of exposure to DNA damaging agents.

Keywords

References

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