• YAKHAK HOEJI
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• v.36 no.2
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• pp.167-172
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• 1992

Klebsiella K-36 producing novel sulfotransferase was isolated from rat intestinal flora. The novel sulfotransferase catalyzed the transfer of sulfate group from p-nitrophenylsulfate to phenolic compounds but it did not use PAPS(3'-phosphoadenosine 5'-phosphosulfate) as a donor substrate. The present enzyme was 160 K daltons. Optimal pH was 10. When p-nitrophenyl sulfate was used as a donor substrate, 1-naphthol was the best substrate, followed by phenol, phenanthrol and tyrosine. The apparent Km for phenol using p-nitrophenylsulfate as a donor substrate and that for p-nitrophenylsulfate using phenol as an acceptor substrate were determined to be 0.66 mM and 0.11 mM, respectively.

• Korean Chemical Engineering Research
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• v.55 no.2
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• pp.237-241
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• 2017

Acetoin is variously applicable platform chemical in chemical and food industry. In this study, Klebsiella pneumoniae was engineered for acetoin production using metabolic engineering. From the recombinant Klebsiella pneumoniae (KMK-05) producing 2,3-butanediol, budC and dhaD genes encoding two 2,3-butanediol dehydrogenases were deleted to reduce 2,3-butanediol production. Furthermore, a transcriptional regulator, AcoK, was deleted to reduce the expression levels of acetoin degrading enzyme. Lastly, NADH oxidase was overexpressed for adjusting intracellular redox balance. The resulting strain (KJW-03-nox) produced considerable amount of acetoin, with concentration reaching 51 g/L with 2.6 g/L/h maximum productivity in 36 h fed-batch fermentation.

• The Journal of the Korean Society for Microbiology
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• v.22 no.4
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• pp.427-433
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• 1987

One hundred and thirty-six strains of Klebsiella pneumoniae were isolated from clinical specimen taken from pediatric patiants at 6 different hospitals and identified, characterized and investigated on the patterns of antibiotic resistance. The 136 strains showed the positive reactions in the 17 tests; Voges-Proskauer, ONPG, cirate utilization, KCN degradation, productions of lysine decarboxylase, acid and gas from glucose, utilizations of malonate, manitol, rhamnose, salicin, sucrose, raffinose, arabinose, lactose, sucrose, inositol and raffinose, but the strains showed the negative reactions in the 8 tests; production of $H_2S$, indole, arginine dehydrolase, ornithine decaraoxylase, phenylalanine deaminase, motility, methly red and gelatin liquefaction. 41 did not utilize dulcitol, and 32 did not utilize adonitol. The 36 out of them produced klebecin. The 136 strains were resistant to ampicillin, 2 to gentamicin, 14 to cephalothin, 16 to chloramphenicol, 8 to kanamycin, 13 to streptomycin, and 17 to tetracycline. Twenty strains were resistant to 2 kinds of antibiotics 5 strains to 3, 4 to 4 and 1 to 6 and 7.

• Journal of Veterinary Clinics
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• v.36 no.5
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• pp.245-247
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• 2019

Taylorella equigenitalis (T. equigenitalis), Klebsiella pneumoniae (K. pneumoniae), and Pseudomonas aeruginosa (P. aeruginosa) are sexually transmittable bacteria known to cause venereal diseases (VD) in horses. T. equigenitalis causes contagious equine metritis (CEM), which is a considerable concern for equine breeding industry. K. pneumoniae and P. aeruginosa may cause endometritis and infertility in susceptible mares. The purpose of this study was to investigate the prevalence of these bacteria among breeding Thoroughbreds in South Korea. External genital swabs were collected from 178 breeding Thoroughbreds, including 11 stallions and 167 mares. The samples were tested using a commercial multiplex real-time PCR kit. T. equigenitalis, P. aeruginosa, and K. pneumoniae were present in 5.6%, 7.3%, and 5.6% of tested Thoroughbreds, respectively. The results highlight the need for regular testing of South Korean Thoroughbreds, particularly those used for breeding, for these bacteria. The regular pre-breeding test for these bacteria will prevent health complications for the horse and financial losses for the owner as a result of VD.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1992.05a
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• pp.23-23
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• 1992

횐 쥐에서 sulfotransferase 양성균주를 검색한 결과 호기성 조건보다는 혐기성 조건의 균이 많은 것으로 나타났다. 이러한 곁과는 장내미생물의 대부분이 혐기성균이라는 것과도 일치하는 것이다. 검색용 배지에서 형광을 나타내는 Sulfotransferase를 생산하는 4개 균주에서 가장 활성이 높은 k-36균주에 대하여 동정하였다. K-36 균주는 혐기적 및 호기적 양조건에서 잘 자라는 통성혐기성균이고, Gas를 생산하며 포자를 생성하지 않는 Gram음성의 간균이었다. 이것으로 보아 이균은 Enterobacteriacease의 Klebsiella oxytoca 로 동정했다. K-36에서 분리한 Sulfotransferase의 반응양식은 p-nitrophenylsulfate와 p-nitrophenol이 반응시간과 함께 동량씩 감소하여 반응 생성물인 phenylsulfate와 p-nitrophenol을 생성시켰으며 sulfatase 반응은 진행되지 않았다.

• Korean Journal of Food Science and Technology
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• v.31 no.1
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• pp.260-262
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• 1999

Abies koreana Wilson is a proper tree to Korea. We tested the antimicrobial activity of the lignans isolated from it. The lignans inhibited the growth of Klebsiella pneumoniae and Vibrio parahaemolyticus at the concentration of $10^3\;ppm\;and\;10^4\;ppm$. Especially, the compound V showed higher inhibition activity to Vibrio parahaemolyticus by 1.7 times compared with the control. The compound III showed activity of 36% of the control to Klebsiella pneumoniae. The compounds II and VI had the high growth inhibition activity of 48, 66% respectively to Vibrio parahaemolyticus.

• Journal of Microbiology and Biotechnology
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• v.20 no.5
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• pp.935-941
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• 2010

The emergence of antibiotic-resistant bacterial strains is one of the most critical problems of modern medicine. Bacteriophages have been suggested as an alternative therapeutic agent for such bacterial infections. In the present study, we examined the therapeutic potential of phage Kpn5 in the treatment of Klebsiella pneumoniae B5055-induced burn wound infection in a mouse model. An experimental model of contact burn wound infection was established in mice employing K. pneumoniae B5055 to assess the efficacy of phage Kpn5 in vivo. Survival and stability of phage Kpn5 were evaluated in mice and the maximum phage count in various organs was obtained at 6 h and persisted until 36 h. The Kpn5 phage was found to be effective in the treatment of Klebsiella-induced burn wound infection in mice when phage was administered immediately after bacterial challange. Even when treatment was delayed up to 18 h post infection, when all animals were moribund, approximately 26.66% of the mice could be rescued by a single injection of this phage preparation. The ability of this phage to protect bacteremic mice was demonstrated to be due to the functional capabilities of the phage and not due to a nonspecific immune effect. The levels of pro-inflammatory cytokines (IL-$1{\beta}$ and TNF-${\alpha}$) and anti-inflammatory cytokines (IL-10) were significantly lower in sera and lungs of phage-treated mice than phage untreated control mice. The results of the present study bring out the potential of bacteriophage therapy as an alternate preventive approach to treat K. pneumoniae B5055-induced burn wound infections. This approach not only helps in the clearance of bacteria from the host but also protects against the ensuing inflammatory damage due to the exaggerated response seen in any infectious process.

• Food Science of Animal Resources
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• v.29 no.4
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• pp.533-538
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• 2009

To suggest an improved diagnostic method for Salmonella spp., analyses were conducted with immunoliposomes and compared with the results from a commercial test kit. One sample out of 36 samples of eggshell was Salmonella-positive via immunoliposomes. In the case of the use of the commercial test kit, six samples out of 36 samples were Salmonellapositive. These Salmonella-positive samples were subjected to biochemical identification tests that confirmed that they were Salmonella-negative. As for the egg content samples, they were Salmonella-negative in both analyses with immunoliposomes and the commercial test kit. The Salmonella analysis with immunoliposomes reduced detection time, by 24 h compared to the commercial test kit. Bacteria, including Acinetobacter baumanni, Chryseomonas luteola, Enterobacter cloacae, Escherichia coli, Escherichia hermannii, Klebsiella pneumonia, Pantoea spp., and Pasteurella pneumotropica, were isolated from the eggshells. Other than Acinetobacter baumanni and Pasteurella pneumotropica most of the isolates were known to frequently appear during egg production processing.

• Korean Journal of Microbiology
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• v.34 no.1_2
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• pp.31-36
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• 1998

A broad spectrum of antibiotic action was studied with Centipedin purified from centipede Scolopendra subspinipes multilans L. Koch aganist gram-positive, gram-negative bacteria and fungi. The minimal inhibitory concentrations(MICs) were determined in liquid medium. The significant antibiotic activity was obtained aganist gram-negative Klebsiella pneumoniae ATCC 8308 responsible for causing infection at lung and intestine. The MIC value against Klebsiella pneumoniae ATCC 8308 was $2{\mu}g/ml$, and this Centipedin was active against Proteus vulgaris NRRL B-123. In addition, it has been shown that Centipedin blocks procaryotic RNA transcription and a little of DNA replication system in vitro. Centipedin did not exhibit any significant cytotoxicity against animal cells such as human blood leukemia (HL-60) and mouse B lymphocyte myeloma cell.

• Journal of Microbiology and Biotechnology
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• v.22 no.6
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• pp.872-881
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• 2012

The inhibitory effect of the Klebsiella pneumoniae ATCC 13883 strain caused by the hexane extract of Halimeda discoidea (Nor Afifah et al., 2010) was further evaluated by means of the microscopy view and its growth curves. The morphological changes of the K. pneumoniae ATCC 13883 cells were observed under the scanning electron microscope (SEM) and transmission electron microscope (TEM) after they were treated at minimum inhibitory concentration (MIC; 0.50 mg/ml) (Nor Afifah et al., 2010) for 12, 24, and 36 h. The results showed the severity of the morphological deteriorations experienced by the treated cells. The killing curve assay was performed for 48 h at three different extract concentrations (1/2 MIC, MIC, and 2 MIC). An increase in the extract concentration of up to 2 MIC value did significantly reduce the number of cells by approximately 1.9 $log_{10}$, as compared with the control. Identification of the potential compounds of the extract responsible for the antibacterial activity was carried out through the gas chromatography-mass spectrum (GC-MS) analysis of the active subfraction, and the compound E-15-heptadecenal was identified and suggested as the most potential antibacterial compound of this extract. The subsequent cellular degenerations showed by the data might well explain the inhibitory mechanisms of the suggested antibacterial compound. All of these inhibitory effects have further proven the presence of an antibacterial compound within H. discoidea that can inhibit the growth of K. pneumoniae ATCC 13883.