• 제목/요약/키워드: Kinetic Method

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Kinetic Parameters 결정을 위한 Equivalent Point Method의 이용 (Application of the Equivalent Point Method for Estimation of Kinetic Parameters)

  • 임종환
    • 한국식품과학회지
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    • 제22권5호
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    • pp.582-589
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    • 1990
  • 본 고에서는 Equivalent time과 Equivalent temperature를 활용하여 Kinetic parameters를 결정하는 새로운 방법을 제안하였다. 본 방법의 타당성을 두 가지의 kinetic data 즉, 계산치와 실험치를 이용하여 예시하였다. 계산치는 그 Kinetics가 잘 알려진 세 가지 화학반응에 대해 임의의 등온가열조건을 적용하여 계산하였고 실험치는 2% 설탕용액을 사용하여 0.0005N 염산용액을 사용하여 가수분해가 일어나는 정도를 효소반응을 이용하여 측정하였다. 본 방법에 의해 결정된 활성화 에너지와 Frequency factor는 각각 $104.74{\pm}1.87KJ/mol$$5.26{\times}10^{14)hr^{-1}$이었으며 이들 값은 보고된 결과와 잘 일치되었다.

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발열성 물질시험과 세균 내독소 시험의 비교 연구 (Comparison of LAL Test with Pyrogen Test)

  • 이유경;강윤숙;백선영;김용관;신광훈;민홍기
    • 약학회지
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    • 제43권5호
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    • pp.606-613
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    • 1999
  • To survey the possibility of replacing the pyrogen test with Limulus Amebocyte Lysate(LAL) test and to find out a standard methods suitable to our blood products made in Korea, 100 samples of 20% human serum albumin were tested by commercial LAL test kits and results of those were compared with rabbit pyrogen test. The LAL test is used both dinetic-chromogenically and kinetic-turbidimetrically. Both methods equally showed broad detection range (5.0~0.005 EU/ml), excellent sensitivity ($\geq$ 0.005 EU/ml) and predominant recovery rate within valid dilution range, but kinetic-turbidimetric method seemed to be more reproducible than kinetic-chromogenic method(kinetic-chromogenic method : S.D. = 15.88, kinetic-turbidimetric method : S.D. = 8.12). After heating the sample at 75$^{\circ}C$ for 15 min, the results showed a little elevated recovery rate with both methods. After performing the test on 100 albumin samples with both kits, the results were analysed using the USP standard (1.33 EU/ml). 7% of samples in kinetic-chromogenic methods and 1% of samples in kinetic-turbidimetric method exceeded the limit of endotoxin levels regulated for blood products in USA. Because this phenomenon was not observed in both methods at the same time and both methods have high sensitivity ($\geq$0.005 EU/ml), these results seemed to depend on nonspecific reaction. Considering its sensitivity and reproducibility, we could assure that LAL test is proper to detecting pyrogenic with good sensitivity.

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개에 있어서 약물에 의한 당뇨병의 유발과 혈당제어의 감시에 관한 연구 1. 세 가지 Fructosamine측정법의 비교 실험 (Drug-Induced Diabetes Mellitus and Monitoring Hyperglycemic Control in Dogs 1. Comparison of Three Methods of Measuring Fructosamine)

  • 이창우;최희인;김본원
    • 한국임상수의학회지
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    • 제15권2호
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    • pp.279-290
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    • 1998
  • The diagnosis of canine diabetes mellitus is generally made by measuring serum fructosamine levels. The Rosental's end points Tas and El Din's kinetic, and Johnson and colleagues'kinetic techiniques were compared for the measuring serum fructosamine levels. In the Rosental's end point methods within-run and between-run coefficient of variability (CV) were 2.4-3.5% and 6.8-8.5%, respectively, and in the Tas and El Din's kinetic method, within-run and between-run CV were 1.3-3.0% and 3.4-3.9%, respectively. In the Johnson and colleagues'kinetic methods within-run and between-run CV were 0-1.8% and 3.1-6.8%1 ruts- pectively. Hyperlipidemia induced by oral administration of 3 ml of corn oil per kilogram body weight did not affect serum fructosamine concentration significantly in the Johnson and colleagues' kinetic method Johnson and colleagues'kinetic method is most reliable and consistant on the basis of comparison study.

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Validation of Kinetic Method for the PKA Assay in Plasma-Derived Products

  • Shin, In-Soo;Hong, Choong-Man;Koh, Hyun-Chul;Hong, Seung-Hwa
    • Biomolecules & Therapeutics
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    • 제13권1호
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    • pp.59-63
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    • 2005
  • A kinetic assay was carried out in order to compare the ability of detection for prekallikrein activator(PKA) in plasma-derived products with that of an endpoint assay and a commercial method. Using these methods, 9 human albumin preparations were assayed and compared to each other. The coefficient of variation between the Kinetic assay and the end point assay was found within 6.6% and this result showed that two methods were highly correlative and the end point assay could act as a replacement of the kinetic assay. Another important goal of this study was to investigate the reproducibility among laboratories on the kinetic assay. A collaborative study was performed to validate the kinetic method with intra and inter assays. The coefficient of variation for the intra assay of each laboratory was less than 4% and that for between individuals in the inter assay was 4.1%. These results revealed that the kinetic assay showed good reproducibility. The contents of PKA in plasma-derived products were also determined by the kinetic assay. As a result, it was found that trace amounts of PKA were present in 32 human immunoglobulin preparations, however the average concentration of PKA in 171 albumin preparations was 5.8 IU/mL.

Design Parameter Structure for Architectural Elements of External Kinetic Facade

  • Ji, Seok-Hwan;Lee, Byung-Yun
    • KIEAE Journal
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    • 제16권3호
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    • pp.35-46
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    • 2016
  • Purpose: This paper aims to analyse the composition system of architectural elements including shape, kinetic and material elements of kinetic facades and establish the design parameter system as a common conceptual and practical knowledge sharing platform with mechanical and electrical experts. Method: This research has been conducted in a three steps. At first, 120 cases of external shading devices are analyzed and their classification criteria have been established. Secondly geometric, kinetic and material elements are categorized in a common kinetic facade coordinates system considering environmental effects and operation method, and the applicability of combination of each element are tested. Lastly core design parameters for each element have been established in a common office building installation coordinate. Result: Geometry elements are categorized into seven geometric shapes and kinetic elements is categorized into basic linear and rotational motion and combinational folding and rolling motion. The combined set of parameters for three elements composes the whole design parameters for architectural elements of kinetic façade. Design parameters of shape elements are composed of shape, installation and arrangement parameters; design parameters for kinetic elements are composed of axis and range parameters; and design parameters of material elements are composed of thermal, lighting and color parameters.

The Theoretical Calculations of Kinetic and Thermodynamics Parameters and Anharmonic Correction for the Related Reactions of NO3

  • Yu, Hongjing;Liu, Yancheng;Xia, Wenwen;Wang, Li;Jiang, Meiyi;Hu, Wenye;Yao, Li
    • 대한화학회지
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    • 제65권6호
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    • pp.419-432
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    • 2021
  • According to the transition state (TS) theory, Gaussian software and Yao and Lin (YL) method, the thermodynamics and kinetic data respectively were calculated, and anharmonic effect was considered for related reactions of NO3. The methods of calculating and fitting kinetic and thermodynamics parameters were provided by least square method and related equations in this paper. Notably, the fitted E of Arrhenius equation was close to the calculated barrier of related reaction by QCISD(T) method. Therefore, the kinetic fitting result can well express the physical meaning of E in Arrhenius equation. Besides, the conversion process and the reaction mechanism of NO3 were researched. For NO3, it seemed that its instability results from its easy reaction with other substances rather than the decompose reaction of itself.

가우시안 랜덤매질을 통과한 광도변동의 시뮬레이션 (Numerical Simulation of Irradiance Scintillation through a Gaussian Random Medium)

  • Jeong Ki Pack
    • 전자공학회논문지A
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    • 제29A권11호
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    • pp.40-48
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    • 1992
  • The wave-kinetic numerical method is used in simulating the irradiance scintillations of optical wave through a two-dimensional random medium containing weak Gaussian fluctuations of the refractive index. The results are compared to existing analytical or numerical results. The wave-kinetic method is a phase-space ray-tracing method for certain key ray trajectories, and the irradiance is calculated by reconstructing the entire beam from these trajectories. The strength of the wave-kinetic method lies in the fact that it can be applied to any type of random media.

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Comparison of the Cross-Sectional Area of Longus Colli and Muscle Activity of Sternocleidomastoid in Subjects With Forward Head Posture on the Two Craniocervical Flexion Methods

  • Son, Min-chan;Hwang, Ui-jae;Jung, Sung-hoon;Ahn, Sun-hee;Kim, Hyun-a;Kwon, Oh-yun
    • 한국전문물리치료학회지
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    • 제25권2호
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    • pp.62-70
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    • 2018
  • Background: The craniocervical flexion (CCF) exercise is one of the effective exercise in correcting forward head posture (FHP). However, some people with FHP achieve CCF with compensatory movements, for example, low cervical flexion using superficial neck flexors such as the sternocleidomastoid (SCM) muscle. No study has yet investigated whether a dual­pres ure biofeedback unit (D-PBU) method to prevent low cervical flexion would be helpful in performing pure CCF movement. Objects: The purpose of this study was to compare the effects of the CCF using D-PBU method and the traditional CCF method on the cross-sectional area (CSA) of the longus colli muscle (LCM) and the activity of SCM muscle in subjects with FHP. Methods: Twenty­four FHP subjects (male: 16, female: 8) were recruited for this study. All subjects performed CCF using two different methods: the traditional CCF method and the CCF using D-PBU method. The CSA of the LCM was measured via ultrasound, and surface electromyography was used to measure SCM muscle activity. Results: The change in CSA of the LCM was significantly larger during the CCF using D-PBU method ($1.28{\pm}.09$) compared with the traditional CCF method ($1.19{\pm}.08$) (p<.05). The SCM muscle activity using the CCF using D-PBU method ($2.01{\pm}1.97$ %MVIC) was significantly lower than when using the traditional CCF method ($2.79{\pm}2.32$ %MVIC) (p<.05). Conclusion: The CCF using D-PBU method can be recommended for increasing LCM activation and decreasing SCM muscle activity during CCF movement in subjects with FHP.

Kinetic Energy Release in the Fragmentation of tert-Butylbenzene Molecular Ions. A Mass-analyzed Ion Kinetic Energy Spectrometric (MIKES) Study

  • Choe, Joong-Chul;Kim, Byung-Joo;Kim, Myung-Soo
    • Bulletin of the Korean Chemical Society
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    • 제10권2호
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    • pp.167-171
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    • 1989
  • Kinetic energy release in the fragmentation of tert-butylbenzene molecular ion was investigated using mass-analyzed ion kinetic energy spectrometry. Method to estimate kinetic energy release distribution (KERD) from experimental peak shape has been explained. Experimental KERD was in good agreement with the calculated result using phase space theory. Effect of dynamical constraint was found to be important.

진공상온분사(VKS) 공정에서의 비행입자 가속 기구 및 속도제어를 위한 가스 유량 효과에 관한 연구 (Research on Acceleration Mechanism of Inflight Particle and Gas Flow Effect for the Velocity Control in Vacuum Kinetic Spray Process)

  • 박형권;권주혁;이일주;이창희
    • 한국재료학회지
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    • 제24권2호
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    • pp.98-104
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    • 2014
  • Vacuum kinetic spray(VKS) is a relatively advanced process for fabricating thin/thick and dense ceramic coatings via submicron-sized particle impact at room temperature. However, unfortunately, the particle velocity, which is an important value for investigating the deposition mechanism, has not been clarified yet. Thus, in this research, VKS average particle velocities were derived by numerical analysis method(CFD: computational fluid dynamics) connected with an experimental approach(SCM: slit cell method). When the process gas or powder particles are accelerated by a compressive force generated by gas pressure in kinetic spraying, a tensile force generated by the vacuum in the VKS system accelerates the process gas. As a result, the gas is able to reach supersonic speed even though only 0.6MPa gas pressure is used in VKS. In addition, small size powders can be accelerated up to supersonic velocity by means of the drag-force of the low pressure process gas flow. Furthermore, in this process, the increase of gas flow makes the drag-force stronger and gas distribution more homogenized in the pipe, by which the total particle average velocity becomes higher and the difference between max. and min. particle velocity decreases. Consequently, the control of particle size and gas flow rate are important factors in making the velocity of particles high enough for successful deposition in the VKS system.