• Title/Summary/Keyword: Kil Jae

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A Case of Multiple Large Renal Abscesses Completely Resolved by Conservative Antibiotics Administration (보존적 항생제 요법으로 완치된 다발성 거대 신농양 1례)

  • Park Jae-Ryun;Oh Jin-Won;Kim Pyung-Kil;Yoo Hwang-Jae
    • Childhood Kidney Diseases
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    • v.10 no.1
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    • pp.77-82
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    • 2006
  • Renal abscess in childhood is a rare disease, and generally treatment of renal abscesses in childhood follows the guidelines in adults. The guidelines of treatment of renal abscesses in adults include the following : renal abscesses smaller than 3 cm in size can be managed by antibiotics administration, while renal abscesses above 3 cm in size must be considered for percutaneous abscess drainage or open drainage. We experienced a case of a 2 year-old girl with multiple renal abscesses greater than 4 cm in size which resolved by oral antibiotics administration after 2 weeks of intravenous administration. We report this case with literature review.

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Building test bed of submerged gabion groyne to prevent bank erosion of curved channel (하천 만곡부 세굴 방지를 위한 잠형 수제 Test bed 구축)

  • Kim, Chang Sun;Lim, Dae Sik;Chung, Sun Kil;Lee, Hyun Jae;Hyun Jae, Lee
    • Proceedings of the Korea Water Resources Association Conference
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    • 2019.05a
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    • pp.86-86
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    • 2019
  • 일반적으로 하천에 설치하는 각종 이수 및 치수 시설물들은 각각의 고유기능을 충분히 발휘하도록 계획하고 설치하고 있으나, 이러한 하천 시설물 중에는 하천의 자연 환경에 부정적인 영향을 일으킬 수 있는 환경과 조화롭지 못한 시설물이 있기도 하다. 하도 만곡부에 설치하는 전통적인 수제, 즉 하안부에서 수로 중심방향으로 돌출하는 형식의 횡수제, 역시 외곽부 하안의 침식을 방지하기 위해 설치하는 시설물이지만 하천의 자연환경 측면에서는 바람직하지 못 한 현상을 일으키기도 한다. 본 연구에서 소개하는 잠형의 방틀공 수제는 하안부에 돌출하여 설치하는 기존 수제와 달리 만곡부 외측 하안에 근접하여 유수 방향으로 하상에 고정시키고 홍수 시에는 잠길 수 있는 높이로 방틀공을 설치하여 하천수의 흐름을 제어하는 시설물이다. 이와 같은 시설물은 만곡부에서 발생하는 2차류 흐름을 방지하고 유수의 수충력을 흡수 혹은 반사시킴으로써 하안부를 세굴로부터 보호할 뿐만 아니라 심각한 하상 세굴과 퇴적을 완화하는 하도의 안정 기능도 발휘하게 된다. 치수의 기능 외에도 하천 환경 측면에서는 수제의 안쪽에 형성되는 못 (pool)에 다양한 생물이 서식하는 서식기능을 담당하기도 한다. 본 고에서는 현재까지 국내에 설치한 사례가 없는 잠형 방틀형 수제의 효과를 검증하기 위해 강원도 양양 남대천에 구축하는 Test bed를 소개하고자 한다. 산지하천의 만곡부 하안부를 세굴로부터 보호하기 위해 설치하는 잠형 수제에 대한 Test bed 구축 배경과 설치 과정 등을 소개하여 이와 관련한 하천 기술자들에게 새로운 패러다임의 수제 설계가 이루어질 수 있는 계기를 제공하고자 한다.

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A New Protocol for Effective Cryopreservation of Human Embryonic Stem Cells by a Minimum Volume Cooling Method

  • Kim, Eun-Young;Lee, Keum-Sil;Shin, Hyun-Ah;Park, Sae-Young;Yoon, Ji-Yeon;Kil, Kwang-Soo;Lee, Young-Jae;Kim, Nam-Hyung;Chung, Kil-Saeng
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.98-98
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    • 2003
  • Recently, human embryonic stem (hES) cells have become very important resources for ES cell basic research, cell replacement therapy, and other medical applications; thus, efficient cryopreservation methods for these cells are needed. This study examined whether a newly developed minimum volume cooling (MVC) vitrification method, which was tested through cryopreservation of sensitive bovine oocytes, can be used for freezing hES cells. Feeder-free cultured hES cell (MB03) colonies were mechanically dissected into several small clumps following enzymatic treatment. We compared the freezing efficiency of a slow-cooling method using a cryo-module (0.4-0.6C/min, 20-30 clumps/vial) and MVC vitrification using a modified 0.5-ml French mini-straw designated as a MVC straw (>$20,000{\circ}C$/min, 10 clumps/straw) After thawing, in vitro survival of hES cell clumps was higher for MVC-vitrified cells (80.8%, 97/120) than for slow-cooled cells (38.2%, 39/102). Further, the proliferation rate of surviving MVC-vitrified cells was similar to that of control hES cells from 2 weeks after thawing. In addition, vitrified-thawed hES cells demonstrated a normal karyotype, were positively immunostained for surface marker antibodies (AP, SSEA-4 and TRA-1-60) and the Oct-4 antibody, and could differentiate into all three embryonic germ layer cells in vitro. This result demonstrates that hES cell clumps can be successfully cryopreserved by a newly developed MVC vitrification method without loss of human cell characteristics.

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A study on the measurement of the nucleated red blood cell (nRBC) count based on birth weight and its correlation with perinatal prognosis in infants with very low birth weights

  • Kil, Tae-Hwan;Han, Ji-Yeon;Kim, Jun-Bum;Ko, Gyeong-Ok;Lee, Young-Hyeok;Kim, Kil-Young;Lim, Jae-Woo
    • Clinical and Experimental Pediatrics
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    • v.54 no.2
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    • pp.69-78
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    • 2011
  • Purpose: The aim of this study was conducted to investigate the mean nRBC count in very low births weight infants (VLBWIs) and to determine the usefulness of the nRBC as an independent prognostic factors of perinatal complications in VLBWIs. Methods: This study was conducted on 112 VLBWIs who were hospitalized in the neonatal intensive care unit (NICU) of the author's hospital within the period from March 2003 to and May 2008. Based on the infants' nucleated red blood cells (nRBC) counts at birth, on the third day after birth, on the seventh day after birth, in the second week after birth, and in the fourth week after birth in the medical records, the correlation between nRBC or absolute nRBC counts with birth weight, gestational age, and other perinatal outcomes were retrospectively investigated. Results: In VLBWIs, their mean nRBC and absolute nRBC counts were showing a gradual decrease after birth, and they were consisteantly kept at low values since one week after and inversely proportional to the birth weights. The mean nRBC counts based on the stage after birth showed a significant correlation with perinatal death, necrotizing enterocolitis, and severe intraventricular hemorrhage. Conclusion: The increase in the nRBC count showed a significant correlation with having a severe intraventricular hemorrhage, necrotizing enterocolitis, and perinatal death in VLBWIs. If an increase or no decrease in the nRBC count after birth is observed, newborninfant care precautions should be required.

Carrier-enhanced Ferromagnetism in Cr-doped ZnO (Cr이 치환된 ZnO에서 나르개에 의한 강자성의 향상)

  • Sim, Jae-Ho;Kim, Hyo-Jin;Kim, Do-Jin;Ihm, Young-Eon;Yoon, Soon-Kil;Kim, Hyun-Jung;Choo, Woong-Kil
    • Journal of the Korean Magnetics Society
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    • v.15 no.3
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    • pp.181-185
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    • 2005
  • We have investigated the effects of Al codoping on the structural, electrical transport, and magnetic properties of oxide diluted magnetic semiconductor $Zn_{1-x}Cr_xO$ thin films prepared by reactive sputtering. Nondoped $Zn_{0.99}Cr_{0.01}O$ thin films show semiconducting transport behavior and weak ferromagnetic characteristic. The Al doping increases the carrier concentration and results in an decrease of resistivity and metal-insulator transition behavior. With increasing carrier concentration, the magnetic properties drastically change, exhibiting a remarkable increase of the saturation magnetization. These results show carrier-enhanced ferromagnetic order in Cr-doped ZnO.

Response of Growth and Photosynthesis of Pepper Seedlings to Potassium Fertilizer (칼리처리가 고추 플러그묘의 생육과 광합성에 미치는 영향)

  • Ahn, Chong-Kil;Son, Beung-Gu;Kang, Jum-Soon;Lee, Yong-Jae;Chang, Min-Kyu;Son, Kil-Woo;Park, Yaung-Hoon;Choi, Young-Whan
    • Journal of Bio-Environment Control
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    • v.12 no.1
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    • pp.26-29
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    • 2003
  • Experiments were conducted to estimate optimal potassium strength in the nutrient solution for ‘Nokgwang’and‘Kwari’green pepper seedlings cultured in small plug-cell trays. Plant height, stem diameter, and leaf area increased with increasing K concentration, with greater effect on ‘Kwari’ than ‘Nokgwang’. Total dry weight increased by increasing potassium strength.‘Kwari’had heavier dry weight than ‘Nokgwang’Chlorophyll content differed by cultivars and potassium strength. The highest chlorophyll content was obtained at 2.0 strength potassium in‘Nokgwang’, but not in‘Kwari’Net photosynthesis was greater in plants fertilized with 1.5 strength potassium for‘Nokgwang’and 2.0 strength potassium for ‘Kwari’than the other treatments.

Metabolic profiling reveals an increase in stress-related metabolites in Arabidopsis thaliana exposed to honeybees

  • Baek, Seung-A;Kim, Kil Won;Kim, Ja Ock;Kim, Tae Jin;Ahn, Soon Kil;Choi, Jaehyuk;Kim, Jinho;Ahn, Jaegyoon;Kim, Jae Kwang
    • Journal of Applied Biological Chemistry
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    • v.64 no.2
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    • pp.141-151
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    • 2021
  • Insects affect crop harvest yield and quality, making plant response mechanisms to insect herbivores a heavily studied topic. However, analysis of plant responses to honeybees is rare. In this study, comprehensive metabolic profiling of Arabidopsis thaliana exposed to honeybees was performed to investigate which metabolites were changed by the insect. A total of 85 metabolites-including chlorophylls, carotenoids, glucosinolates, policosanols, tocopherols, phytosterols, β-amyrin, amino acids, organic acids, sugars, and starch-were identified using high performance liquid chromatography, gas chromatography-mass spectrometry, and gas chromatography-time-of-flight mass spectrometry. The metabolite profiling analysis of Arabidopsis exposed to honeybees showed higher levels of stress-related metabolites. The levels of glucosinolates (glucoraphanin, 4-methoxyglucobrassicin), policosanols (eicosanol, docosanol, tricosanol, tetracosanol), tocopherols (β-tocopherol, γ-tocopherol), putrescine, lysine, and sugars (arabinose, fructose, glucose, mannitol, mannose, raffinose) in Arabidopsis exposed to honeybees were higher than those in unexposed Arabidopsis. Glucosinolates act as defensive compounds against herbivores; policosanols are components of plant waxes; tocopherols act as an antioxidant; and putrescine, lysine, and sugars contribute to stress regulation. Our results suggest that Arabidopsis perceives honeybees as a stress and changes its metabolites to overcome the stress. This is the first step to determining how Arabidopsis reacts to exposure to honeybees.

Somatic Embryogenesis from In Vitro Grown Leaf Explants of Rosa hybrida L.

  • Kim Chang-Kil;Chung Jae-Dong;Jee Sun-Ok
    • Journal of Plant Biotechnology
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    • v.5 no.3
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    • pp.169-172
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    • 2003
  • Somatic embryogenesis was initiated from in vitro grown leaf explants of rose following an induction period of four weeks on MS basal medium supplemented with auxin and several subcultures on MS medium with cytokinin. '4th of July' showed the highest regeneration frequencies on 1 mg/L NAA followed by culture on medium with 4 mg/L zeatin. The embryogenic callus was propagated on MS medium with NAA, zeatin and $GA_3$. Germination of somatic embryos was achieved on MS medium with 1 mg/L BA. Somatic embryo derived plantlets were hardened and successfully transferred to the greenhouse.

Selection and Identification of a Strain KT-10 Producing the Cathepsin B Inhibitor

  • Han, Kil-Hwan;Do, Jae-Ho;Kim, Sang-Dal
    • Journal of Microbiology and Biotechnology
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    • v.7 no.5
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    • pp.333-340
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    • 1997
  • An actinomycetes, KT-10 isolated from ginseng field in Kyongpook, Korea was selected based on its ability to produce a lysosomal cathepsin B inhibitor. The inhibitor purified from the culture supernatant of the isolate KT-10 showed strong inhibitory effects against cathepsin B as well as against papain when the activities were measured using synthetic substrate, ${\alpha}$-N-benzyloxycarbonyl-L-Iysine p-nitrophenyl ester (CLN) or ${\alpha}$-N-benzoyl-D,L-arginine 2-naphthylamide (BANA). The isolate KT-10 was identified as a species of Streptomyces based on its morphological characteristics and chemotaxonomic data. The TAXON program of Ward was used to identify Streptomyces sp. KT-10 as a strain of Streptomyces luteogriseus belong to cluster 18 of the genus Streptomyces with a Willcox probability 0.999388. The cathepsin B inhibitor was presumed to a novel material composed of a polyhydroxylamine.

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A Study on the Short Circuit Current and Molten Mark Analysis of Polyvinyl Chloride Insulated Flexible Cords (비닐코드의 단락전류 및 용융흔 분석에 관한 연구)

  • Choi, Chung-Seog;You, Sun-Hee;Yoo, Jae-Geun;Shong, Kil-Mok;Kim, Hyang-Kon;Lee, Kyung-Sup
    • Proceedings of the KIEE Conference
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    • 1999.07d
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    • pp.1630-1632
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    • 1999
  • In this paper, we studied the short circuit current and molten mark of polyvinyl chloride insulated flexible cords(VFF). The calory decreased remarkably with increase of current. The surface structure and composition of a strand wire were analyzed by stereoscope, scanning electron microscope (SEM), and energy dispersive x-ray spectroscopy(EDX). The surface of a strand wire showed columnar and void. The intensity of CuL spectrum increased in melted mark.

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