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Preventive Effects of Daekumeumja on Fatty Degeneration of Liver and Immunosuppression Induced by Alcohol (대금음자(對金飮子)가 알코올 투여로 유발된 흰쥐의 간 지방 병증과 면역억제의 예방에 미치는 영향)

  • Kim, Jeong-Ja;Seo, Bu-Il;Choi, Hong-Sik;Kim, Seong-Mo;Woo, Chang-Hoon;Koo, Jin-Suk;Park, Gyu-Ryeol
    • Korean Journal of Oriental Medicine
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    • v.16 no.2
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    • pp.167-179
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    • 2010
  • Objective : The present study has been undertaken to investigate the preventive effects of Daekumeumja on fatty degeneration of liver and immunosuppression induced by alcohol in rats. Method : Except for the normal group, we fed rat on 25% alcohol for 55 days. And Daekumeumja(DK) extract was administrated for the same period. We measured the serum component in rat's blood, weight of internal organs, liver triglyceride contents, histomorphometry and histopathological observation of internal organs. Results : 1. In the change measurement of serum components, DK group(50 mg/kg, 200 mg/kg) showed significant decrease of AST, ALT, albumin, ALP and triglyceride in comparison with those of the alcohol control group. 2. In the change measurement of internal organ's weight, DK group(50 mg/kg, 200 mg/kg) showed significant increase of relative body weights of liver, thymus and spleen in comparison with those of the alcohol control group. 3. DK group(50 mg/kg, 200 mg/kg) showed significant decrease of hepatic triglyceride contents in comparison with those of the alcohol control group. 4. In histomorphometrical changes of liver, DK group(50 mg/kg, 200 mg/kg) showed significant decrease of numbers of hepatocytes occupied by over 10% lipid droplets, percentages of regions occupied by lipid droplets and mean diameters of hepatocytes in comparison with those of the alcohol control group. In histomorphometrical changes of thymus, DK group(50 mg/kg, 200 mg/kg) showed significant increase of lobular thickness and cortex thickness in comparison with those of the alcohol control group. In histomorphometrical changes of spleen, DK group(50 mg/kg, 200 mg/kg) showed significant increase of splenic thicknesses, numbers of white pulps and mean diameters of white pulps in comparison with those of the alcohol control group. 5. In histopathological changes of liver, DK group(50 mg/kg, 200 mg/kg) showed effective inhibition of severe fatty changes in comparison with those of the alcohol control group. In histopathological changes of thymus and spleen, DK group(50 mg/kg, 200 mg/kg) showed effective inhibition of atrophic changes in comparison with those of the alcohol control group. Conclusions : Reviewing these experimental results, it appears that Daekumeumja have pharmaceutical preventive efficacy on fatty degeneration of liver and immunosuppression induced by alcohol in rats.

Residue Levels of Chlorantraniliprole and Ethaboxam in Different Parts of a Head-type Korean Cabbage and Reduction of Residues in Outer Leaves by Water Washing and Heat-treatment (결구 배추의 부위별 Chlorantraniliprole 및 Ethaboxam의 잔류수준과 배추 겉잎의 수세 및 열처리에 의한 잔류량 감소)

  • Kim, Jun-Yeong;Lee, Mi-Gyung
    • The Korean Journal of Pesticide Science
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    • v.18 no.4
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    • pp.330-335
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    • 2014
  • This study was conducted to investigate residue levels of chlorantraniliprole and ethaboxam in inner part and outer leaves of a head-type Korean cabbage. Further, reduction of residues was measured after water washing, blanching and heat-cooking of the outer leaves. For chlorantraniliprole, residues in inner part and outer leaves were <0.01 mg/kg and 0.0757 mg/kg, respectively, in case of three-time spraying 30 days before harvest and <0.01 mg/kg and 1.19 mg/kg, respectively, in case of four-time spraying 10 days before harvest; for ethaboxam, <0.05 mg/kg and 0.216 mg/kg, respectively and <0.05 mg/kg and 1.18 mg/kg, respectively. Chlorantraniliprole and ethaboxam were not detected in inner part of the head-type Korean cabbage. Residue levels in outer leaves were very high as 10-100 fold as those in inner part of the cabbage. Therefore, there is no concern for safety of pesticide residues on kimchi prepared with the inner part of a head-type Korean cabbage. In addition, it needs to be noted that outer leaves should be carefully removed at harvest of the cabbage. Outer leaves water washed and blanched are called as Woogeogi, which is consumed after heat-cooking. In Woogeogi, residue concentrations of two compounds reduced to less than 10%, and further less than 5% by heat-cooking. This indicates that considerable amount of the test pesticide residues in outer leaves of a head-type Korean cabbage removed through water washing, blanching and heat-cooking.

Estimating Unit Load of Non-Point Source Pollutants for Landuse Types in Anseongchun Watershed (안성천 유역의 토지이용별 비점오염원 원단위 산정)

  • Hwang, Byung-Gi
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.5
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    • pp.1026-1033
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    • 2009
  • In this study, we computed the unit load of nonpoint source for the forest, agricultural, and urban representative region in Anseongchun watershed. In addition, Flow-weighted mean concentration (FWMC) that well represents runoff characteristics of storm water during rainfall, was calculated, and runoff pollutants loading was also examined. FWMCs of 1st rainfall, which runoff coefficient was high, had a tendency higher than those of 2nd rainfall. Based on landuse results, pollutant concentration of the non-urban such as forest and agricultural regions was higher than that of urban region. In case of BOD, runoff pollutants loading was calculated as 1,395, 1,623, 2,268kg/d in 1st rainfall for forest, agricultural, and urban regions, respectively, while runoff loading of 2nd rainfall was 503kg/d in forest), 512kg/d in agricultural, and 898kg/d in urban. By landuses, unit load of the urban as 72.7kg/ha/yr was 12 times higher than that of the agricultural as 6.5kg/ha/yr, and 8 times higher than that of the forest as 9.5kg/ha/yr.

Changes in Textural Properties of Jeung-Pyon (Korean Traditional Fermented- and Steamed-Rice Bread) during Storage (증편의 저장 중 조직 특성 변화)

  • Chang, Kyu-Seob;Lee, Jeong-Shik;Choi, Seok-Hyun;Park, Young-Duck
    • Korean Journal of Agricultural Science
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    • v.18 no.2
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    • pp.148-156
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    • 1991
  • This study was carried out to investigate the storage conditions and methods required for commercialization of Jeung-Pyon. The storage characteristics of Jeung-Pyon were examined with Universal Testing Machine (UTM, Instron model 1000) for textural properties during storage of under the conditions such as sealing, nonsealing, and temperatures. The differences in storage characteristics on the different varieties and years of rices were not shown. When the Jeung-Pyon was lost it's quality as a commercial product, the measured value of UTM was increased as hardness 0.74Kg to 1.0Kg, gumminess 0.23Kg to 0.59Kg, chewiness 6Kg.mm to 18Kg.mm, but decreased in adhesiveness 0.65Kg.mm to 0.40Kg.mm, cohesiveness 0.61 to 0.34, springiness 41mm to 32mm. The nonsealed Jeung-Pyon was lost it's quality for storing at room temperature because of the case-hardening after 2 days. In the case of sealed Jeung-Pyon, the case-hardening phenomena by the evaporation of moisture was not occured, therefore it was a significant factor for an extension in sealed Jeung-Pyon. The shelf-life of sealed Jeung-Pyon was 20 days and 5 days respectively during storage at $-20^{\circ}C$ and $20^{\circ}C$. The sealed Jeung-Pyon stored at $5^{\circ}C$ was lost it's quality, in consideration of cold chain this temperature was suitable for retrogradation of starch. The Jeung-Pyon sealed with air and mosture proof packaging material stored at the accelerated condition as 92% R.H. and $35^{\circ}C$ lost it's quality within two or three days.

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Investigation of Proper Spring Harvesting Methods on the Summer Planted Asparagus (Asparagus officinalis L.) in Jeju (제주에서 여름정식한 아스파라거스의 이듬해 적정 수확방법 구명)

  • Seong, Ki-Cheol;Kim, Chun-Hwan;Lee, Jin-Su;Moon, Doo-Kyong;Kang, Kyeong-Hee;Eum, Young-Cheol
    • Journal of Bio-Environment Control
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    • v.18 no.3
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    • pp.280-284
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    • 2009
  • One of the big obstacles to cultivate asparagus was long days taking before first harvesting. This study was carried out to hasten the first harvesting of summer planted asparagus in Jeju. Seedlings were raised for three months and planted June 20th in green house. Harvesting of Spring were separated into non-harvested (control) and harvested (partly-harvesting, completely-harvesting). The first year we could harvest $399kg{\sim}400kg/10a$ in harvesting treatment. Second year's yield was 834kg/10a in partly-harvesting, 825kg/10a in completely-harvesting treatment and 908kg/10a in control. There is no significant difference in second years yield regardless of first year's harvesting methods. The accumulated total yield was increased by 35% (l,229kg/10a) in harvesting treatment from the first spring compared with the control. Marketable yield was increased by 33% (1,116kg/10a) compared with non harvesting in first year (839kg/10a). The result of this study shows that doing harvest of the first year's spring in summer planting asparagus would be desirable for yield and possible to harvest after 8 months planting.

Residues of Ampicillin and Amoxicillin in Olive Flounder Paralichthys olivaceus Following Oral Administration (양식 넙치(Paralichthys olivaceus)에 경구투여한 Ampicillin 및 Amoxicillin의 어류체내 잔류량의 변화)

  • Son, Kwang-Tae;Jo, Mi-Ra;Oh, Eun-Gyoung;Mok, Jong-Soo;Kwon, Ji-Young;Lee, Tae-Seek;Song, Ki-Cheol;Kim, Poong-Ho;Lee, Hee-Jung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.5
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    • pp.464-469
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    • 2011
  • Residues of ampicillin and amoxicillin in the muscles of olive flounder Paralichthys olivaceus were investigated after oral doses of 20 mg/kg body weight/day of ampicillin and 40 mg/kg body weight/day of amoxicillin in a first examination and 40 mg/kg body weight/day of ampicillin and 80 mg/kg body weight/day of amoxicillin in a second examination for 5-6 days. The ranges of seawater temperatures in the $1^{st}$ and $2^{nd}$ examinations were $13.4-15.6^{\circ}C$ and $16.8-21.4^{\circ}C$, respectively. Ampicillin and amoxicillin concentrations were analyzed by high performance liquid chromatography (HPLC). The average recovery rates of ampicillin and amoxicillin in fish samples ranged from 84.8-95.2% and 100.8-103.8%, respectively. Residual concentrations in the olive flounder peaked on the $5^{th}$ day, with average concentrations of 0.075 mg/kg and 0.311 mg/kg in the $1^{st}$ examination, and 0.098 mg/kg and 0.630 mg/kg in the $2^{nd}$ examination for ampicillin and amoxicillin, respectively, with maximum concentrations in muscle. In the $1^{st}$ examination, ampicillin concentrations in olive flounder after withdrawals of 1 and 3 days were 0.041 mg/kg and 0.023 mg/kg, respectively, and amoxicillin concentrations after withdrawals of 1 day and 10 days were 0.172 mg/kg and 0.023 mg/kg, respectively. In the $2^{nd}$ examination, the ampicillin concentrations in olive flounder after withdrawals of 1 and 3 days were 0.041 mg/kg and 0.023 mg/kg, respectively, and amoxicillin concentration after withdrawals of 1 day and 10 days were 0.172 mg/kg and 0.023 mg/kg, respectively. We suggest that the recommended withdrawal periods should be 3 days for ampicillin and 10 days for amoxicillin in the olive flounder.

Effects of Weekly Administration of Implant-type Recombinant Porcine Somatotropin on the Performance and Carcass Characteristics of Finishing Pigs

  • Kim, Y.H.;Jung, H.J.;Park, J.C.;Kwon, O.S.;Chung, C.S.;Ko, Y.D.;Moon, H.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.4
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    • pp.557-561
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    • 2005
  • The present study was undertaken to investigate the effects of weekly administration of implant type recombinant porcine somatotropin (rpST) on the performance and carcass characteristics in finishing pigs. A total of 120 crossbred (Landrace${\times}$Yorkshire${\times}$Duroc) pigs were employed for 11 weeks in a growth trial in experiment. A rpST designed to implant every 7 d was used. Forty pigs, each weighing 75 kg, were allocated into three rpST treatments; control (CONT), implant of rpST from 75 kg (TRT1) or 90 kg (TRT2) of body weight. The CONT pig and pigs in TRT2 from 75 kg to 90 kg were treated without rpST but with placebo. In rpST-treated pigs, each 100 mg and 125 mg of the equivalent rpST was implanted from live weight of 75 kg to 90 kg and from 90 kg to market weight, respectively. Half of the pigs from each treatment were marketed at live weight of 110 kg and the rest at 130 kg. All pigs were allowed ad libitum access to a commercial feed containing 0.94% and 0.88% of lysine from 75 to 110 kg, 110 to 130 kg of body weights, respectively. rpST had no effect on daily gain, while feed efficiency was improved by 7 to 13% (p<0.05) in the rpST-treated groups compared with the CONT. Compared with the CONT, backfat thickness was decreased by 12% (p<0.05) in TRT1 at 110 kg of market weight, and by 23 to 32% (p<0.05) in the rpST-treated groups at 130 kg of market weight, respectively. Lean muscle rate tended to be higher in TRT1 at both 110 kg and 130 kg of market weight, and carcass fat percentage in the rpST-treated groups was decreased by 33 to 46% (p<0.05) compared with the CONT.

Salt Movement of Soils by Runoff in Green House Area (시설재배지 토양의 유거수에 의한 염류의 이동)

  • Kang, Bo-Koo;Kim, Hyun-Ju;Lee, Kyung-Ja;Kim, Jai-Joung;Hong, Soon-Dal
    • Korean Journal of Environmental Agriculture
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    • v.20 no.2
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    • pp.112-115
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    • 2001
  • Salt accumulation and movement by runoff ware studied in runoff resevior lysimeter constructed in a green house located in the area of Cheongju, Chungbuk province. Average runoff ratio of rainfall within period of this experiment was 58%. The average content of cations lost from field soil by runoff was in the order of $Ca^{2+}(27.12\;mg/L\;)$ > $K^+(9.18\;mg/L)$ > $Mg^{2+}(2.53\;mg/L)$ > $Na^+(1.89\;mg/L)$ and in the care anions $SO_4\;^{2-}(63.38\;mg\;/L)$ > $NO_3\;^-(25.40\;mg/L)$ > $Cl^-(4.19\;mg/L)$ > $PO_4\;^{3-}(3.18\;mg/L)$. Amounts of salt movement by runoff $SO_4\;^{2-}(140.2\;kg/ha)$, $Ca^{2+}(59.9\;kg/ha)$, $NO_3\;^-(56.1\;kg/ha)$, $K^+(20.3\;kg/ha)$, $Cl^-(9.3\;kg/ha)$, $PO_4\;^{3-}(7.0\;kg/ha)$, $Mg^{2+}(5.6\;kg/ha)$ and $Na^+(4.2\;kg/ha)$. The loss amount of $PO_4\;^{3-}$ was the lowest among the anions investigated in this experiment. $P_2O_5$ was accumulated on the soil surface due to strong affinity for the sorption site on the soil particle surface.

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Selection of superior breeding colonies of Apis mellifera based on performance-testing (능력검정에 의한 우량 종봉 선발에 관한 연구)

  • Son, Young-Ik;Cha, Yong-Ho;Choi, Kwang-Soo
    • Current Research on Agriculture and Life Sciences
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    • v.19
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    • pp.17-22
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    • 2001
  • The study was conducted to establish the method of performance test of honeybee queens under Korean environment. Colony performances of Apis mellifera were tested based on wintering ability, colony population and honey yield in Sangju, Korea from November, 1999 to October, 2001. The results of performance-testing on the honeybee colonies are summarized as follows: The colony weights measured before and after wintering in 1999~2000 season were averaged $22.6{\pm}2.6kg$ and $20.1{\pm}2.6kg$, respectively, showing the decrease of $2.5{\pm}0.7kg$. During 2000~2001 winter season, the colony weight before wintering was $22.6{\pm}2.3kg$ and was decreased by $2.3{\pm}0.6kg$. The number of combs well covered with bees was $13.1{\pm}4.6$ as measured on the 22nd of May. 2000 and $16.0{\pm}3.8$ on the 21st of May. 2001, respectively. The rate of supering colonies were 50% and 98.5% in 2000 and 2001, respectively. The amount of honey production was $12.7{\pm}8.6kg$ per colony in 2000, and $14.2{\pm}7.6kg$ per colony with unselected queens and $23.5{\pm}4.9kg$ per colony with selected-tested queens in 2001, which were harvested two times during main nectar flow season of black locust, Robinia peseudoacasia.

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Change of Dry Matter Yield and Feed Values According to Different Growth Stages of Italian Ryegrass and Triticale Cultivated in the Central Northern Region (중북부 지역에서 생육단계에 따른 이탈리안 라이그라스와 트리티케일의 건물수량과 사료가치)

  • Lee, Hong-Ju;Byeon, Ji-Eun;Hwang, Sun-Goo;Ryoo, Jong-Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.40 no.1
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    • pp.50-56
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    • 2020
  • The field experiment was carried out to evaluate changes of dry matter productivity and feed value of Italian ryegrass and triticale at different growth stages in Wonju from 2018 to 2019. Italian ryegrass and triticale forages were harvested at an interval of seven days from May to June. The dry matter yield of Italian ryegrass with 7,870 kg/ha harvested on May 16 was significantly higher than that harvested on May 3 and May 9. The dry matter yield of triticale with 12,050 kg/ha harvested on June 7 was significantly higher than that harvested from May 3 to May 16. The crude protein yields harvested on May 16 were 890 kg/ha for Italian ryegrass and 1,103 kg/ha for triticale and were significantly higher than those harvested on May 3 and May 9. The total digestible nutrient yield(TDN) of Italian ryegrass with 5,223 kg/ha harvested on May 16 was significantly higher than that harvested on May 3 and May 9. The total digestible nutrient yield of triticale with 8,277 kg/ha harvested on June 7 was significantly higher than that harvested during May, but not different from that harvested after June 7. Considering the dry matter yield, crude protein yield and total digestible nutrient yield, it is suggested that the optimal harvesting times for Italian ryegrass and triticale seem to be May 16 and June 7, respectively.