• Title/Summary/Keyword: Kangwon National University

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Microbiological Studies of Korean Native Soy-sauce Fermentation: A Study on the MicroBora of Fermented Korean Maeju Loaves (한국 재래식간장의 발효미생물에 관한 연구 -한국재래식메주에 발효미생물군에 대하여-)

  • Cho, Duck-Hiyon;Lee, Woo-Jin
    • Applied Biological Chemistry
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    • v.13 no.1
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    • pp.35-42
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    • 1970
  • Five samples of Korean native Maeju(fermented soy-bean mash) loaves which were collected each from Kyunggi, Chungchung, Kangwon, Cholla and Kyungsang-Do were examined for their fermenting microorganisms. The results of taxonomic and ecological studies of fermentation microorganisms in these Maeju loaves were as the fellows. (1) The fungus flora grew only is the outer layer of Maeju loaves. Miscellaneous molds, 3 species of Mucor, 2 species of Pericallium., one species each of Scopulariopsis and Aspergillus, were isolated. None of them seemed exclusively predominant to be able to designate as the ecologically significant. (2) The bacterial flora which consisted of two species, Bacillus subtilis and Bacillus pumilus were distributed uniformly in th a entire Maeju loaves. The inner parts of Maeju loaves were especially inhabited solely by these bacterial flora. Probably the Korean native Maeju fermentation could be characterized by these bacterial flora. A Staphylococcus species was also isolated probably as a casual contaminant. (3) The yeasts, Rhodotorula flava and Torulopsis dattila, were isolated from Maeju loaves though their ecological significance was not clear. (4) The ecological aspects of fermentation microbes in the outer and inner parts of Maeju loaves were apparently different, consequently different fermentation processes might have occurred in these two parts and it brought quite different final outlooks in the final matured Maeju loaves. The outer part, rather rigid and dry, retained the light brown color of boiled soy-bean; whereas the inner part, soft and sticky, showed dark brown color indicating severe chemical changes. (5) The aflatoxin producing mold, Aspergillus oryzae was isolated from one sample among 5 of Maeju loaves. In addition to the low probability of isolability from Maeju loaves samples, since this mold grew only in the outer layer of Maeju loaves with such a low population density, about $10^4/g$, perhaps the aflatoxin problem in Korean native soysauce may not be critical.

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Crosshole EM 2.5D Modeling by the Extended Born Approximation (확장된 Born 근사에 의한 시추공간 전자탐사 2.5차원 모델링)

  • Cho, In-Ky;Suh, Jung-Hee
    • Geophysics and Geophysical Exploration
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    • v.1 no.2
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    • pp.127-135
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    • 1998
  • The Born approximation is widely used for solving the complex scattering problems in electromagnetics. Approximating total internal electric field by the background field is reasonable for small material contrasts as long as scatterer is not too large and the frequency is not too high. However in many geophysical applications, moderate and high conductivity contrasts cause both real and imaginary part of internal electric field to differ greatly from background. In the extended Born approximation, which can improve the accuracy of Born approximation dramatically, the total electric field in the integral over the scattering volume is approximated by the background electric field projected to a depolarization tensor. The finite difference and elements methods are usually used in EM scattering problems with a 2D model and a 3D source, due to their capability for simulating complex subsurface conductivity distributions. The price paid for a 3D source is that many wavenumber domain solutions and their inverse Fourier transform must be computed. In these differential equation methods, all the area including homogeneous region should be discretized, which increases the number of nodes and matrix size. Therefore, the differential equation methods need a lot of computing time and large memory. In this study, EM modeling program for a 2D model and a 3D source is developed, which is based on the extended Born approximation. The solution is very fast and stable. Using the program, crosshole EM responses with a vertical magnetic dipole source are obtained and the results are compared with those of 3D integral equation solutions. The agreement between the integral equation solution and extended Born approximation is remarkable within the entire frequency range, but degrades with the increase of conductivity contrast between anomalous body and background medium. The extended Born approximation is accurate in the case conductivity contrast is lower than 1:10. Therefore, the location and conductivity of the anomalous body can be estimated effectively by the extended Born approximation although the quantitative estimate of conductivity is difficult for the case conductivity contrast is too high.

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Negative apparent resistivity in dipole-dipole electrical surveys (쌍극자-쌍극자 전기비저항 탐사에서 나타나는 음의 겉보기 비저항)

  • Jung, Hyun-Key;Min, Dong-Joo;Lee, Hyo-Sun;Oh, Seok-Hoon;Chung, Ho-Joon
    • Geophysics and Geophysical Exploration
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    • v.12 no.1
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    • pp.33-40
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    • 2009
  • In field surveys using the dipole-dipole electrical resistivity method, we often encounter negative apparent resistivity. The term 'negative apparent resistivity' refers to apparent resistivity values with the opposite sign to surrounding data in a pseudosection. Because these negative apparent resistivity values have been regarded as measurement errors, we have discarded the negative apparent resistivity data. Some people have even used negative apparent resistivity data in an inversion process, by taking absolute values of the data. Our field experiments lead us to believe that the main cause for negative apparent resistivity is neither measurement errors nor the influence of self potentials. Furthermore, we also believe that it is not caused by the effects of induced polarization. One possible cause for negative apparent resistivity is the subsurface geological structure. In this study, we provide some numerical examples showing that negative apparent resistivity can arise from geological structures. In numerical examples, we simulate field data using a 3D numerical modelling algorithm, and then extract 2D sections. Our numerical experiments demonstrate that the negative apparent resistivity can be caused by geological structures modelled by U-shaped and crescent-shaped conductive models. Negative apparent resistivity usually occurs when potentials increase with distance from the current electrodes. By plotting the voltage-electrode position curves, we could confirm that when the voltage curves intersect each other, negative apparent resistivity appears. These numerical examples suggest that when we observe negative apparent resistivity in field surveys, we should consider the possibility that the negative apparent resistivity has been caused by geological structure.

Operating Performance Evaluation of Landfill Facility and Landfill Gas (LFG) Energy Facility (매립시설 및 매립가스 에너지화시설 운영현황 분석)

  • Kim, Jong-Hwan;Phae, Chae-Gun;Park, Joon-Seok
    • Clean Technology
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    • v.24 no.3
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    • pp.221-232
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    • 2018
  • This research was conducted to evaluate the operational performance of landfill facility and landfill gas energy facility from the questionnaire survey. In order to evaluate overall operational performance, three types of weighting methods were applied to each technical, economical, and environmental item. There was no significant difference between an equal weighted method, a weighted method of 40% for technology, 30% for economy, and 30% for environment, and a weighted method of 30% for technology, 40% for economy, and 30% for environment. In technical performance, large and middle scales of landfill facilities showed higher scores of 14.8 ~ 19.7 and 14.3 ~ 19.0 than 9.8 ~ 13.0 of small scale one. In environmental performance, large, middle, and small scales of landfill facilities showed 21.3 ~ 23.7, 17.6 ~ 19.6, and 20.8 ~ 23.1 scores, respectively. However, in economical performance, there was significant difference between them with scale. Large and middle scales of landfill facilities showed higher scores of 22.0 ~ 29.3 and 20.5 ~ 27.3 than 6.0 ~ 8.0 of small scale one. As a result of evaluation for landfill gas energy facility, large scale facility showed 19.2 ~ 25.6 and 17.8 ~ 23.7 scores in technical performance and 23.1 ~ 25.7 and 21.3 ~ 23.7 scores in environmental performance, respectively. However, in economical performance evaluation, large scale of landfill gas energy facility showed relatively higher of 27.8 ~ 33.3 score than 18.8 ~ 25.1 of small scale one. From these results, it was shown that economy evaluation heavily effect on the operational performance of landfill facility and landfill gas energy facility compared to technology and environment evaluation.

Production Medium Optimization for Monascus Biomass Containing High Content of Monacolin-K by Using Soybean Flour Substrates (기능성 원료를 기질로 이용하는 Monacolin-K 고함유 모나스커스 균주의 생산배지 최적화)

  • Lee, Sun-Kyu;Chun, Gie-Taek;Jeong, Yong-Seob
    • KSBB Journal
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    • v.23 no.6
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    • pp.463-469
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    • 2008
  • During the last decade, monacolin-K biosynthesized by fermentation of red yeast rice (Monascus strains) was proved to have an efficient cholesterol lowering capability, leading to rapid increase in the market demand for the functional red yeast rice. In this study, the production medium composition and components were optimized on a shake flask scale for monacolin-K production by Monascus pilosus (KCCM 60160). The effect of three different soybean flours on the monacolin-K production were studied in order to replace the nitrogen sources of basic production medium (yeast extract, malt extract and beef extract). Among the several experiments, the production medium with dietary soybean flour to replace a half of yeast extract was very good for monacolin-K production. Plackett-Burman experimental design was used to determine the key factors which are critical to produce the biological products in the fermentation. According to the result of Plackett-Burman experimental design, a second order response surface design was applied using yeast extract, beef extract and $(NH_4)_2SO_4$ as factors. Applying this model, the optimum concentration of the three variables was obtained. The maximum monacolin-K production (369.6 mg/L) predicted by model agrees well with the experimental value (418 mg/L) obtained from the experimental verification at the optimal medium. The yield of monacolin-K was increased by 67% as compared to that obtained with basic production medium in shake flasks.

A Study on Establishment of the Optimum Mountain Meteorological Observation Network System for Forest Fire Prevention (산불 방지를 위한 산악기상관측시스템 구축방안)

  • Lee, Si-Young;Chung, Il-Ung;Kim, Sang-Kook
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.8 no.1
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    • pp.36-44
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    • 2006
  • In this study, we constructed a forest fire danger map in the Yeongdong area of Gangwon-do and Northeastern area of Gyeongsangbuk-do using a forest fire rating model and geographical information system (GIS). We investigated the appropriate positions of the automatic weather station (AWS) and a comprehensive network solution (a system including measurement, communication and data processing) for the establishment of an optimum mountain meteorological observation network system (MMONS). Also, we suggested a possible plan for combining the MMONS with unmanned monitoring camera systems and wireless relay towers operated by local governments and the Korea Forest Service for prevention of forest fire.

Variation of Samara, Seed, Germination and Growth Characteristics of Ulmus davidiana var. japonica Nakai Populations (느릅나무 자연집단(自然集團)의 시과(翅果), 종자(種子), 발아(發芽) 및 생장특성(生長特性) 변이(變異))

  • Song, Jeong-Ho;Jang, Kyung-Hwan;Lim, Hyo-In;Park, Wan-Geun;Bae, Kwan-Ho
    • Journal of Korean Society of Forest Science
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    • v.100 no.2
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    • pp.226-231
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    • 2011
  • Ulmus davidiana var. japonica is a deciduous tree species used for traditional medicine. This study was conducted to investigate the variation of samara, seed, germination and growth characteristics among populations and among individuals within five natural populations of U. davidiana var. japonica distributed in Korea. The ten characteristics of samara and seed, the three germination behaviors as well as the two growth traits were studied in samaras collected from total 32 trees. Statistical analysis of all characteristics showed that there were significant differences among populations as well as among individuals within populations. In this study, the mean characteristics of this species were 13.0 mm in samara length, 9.7 mm in samara width, 1.37 in samara index, 0.015 g in samara weight, 3.07 mm in samara stalk length, 3.85 seed length, 2.66 mm in seed width, 1.46 in seed index, 1.29 mm seed thickness, 0.0062 g in seed weigh, 34.8% in germination percentage, 8.6 days in mean germination time, 3.5 ea./day in gemination rate, 37.7 cm in height and 4.90 mm in root collar diameter. Especially, coefficients of variations in samara weight, germination percentage, germination rate, height and root collar diameter were relatively high (${\geq}30.0%$) compared to other traits. There was no significant relationship between population association and geographical distribution. The results of principal component analysis for 15 characteristics showed that primary four principal components (PC's) explained 100% of the total variation. The first PC accounted for 41.8% of the variability which correlated with morphological traits, the second PC accounted for 32.9% of the variability which correlated with germination behaviors and the third PC accounted for 16.3% of the variability which correlated with growth traits.

A Study of Depression in Female Seniors Living Alone: A Comparison Between the Young-old and the Old-old Adults (여성 독거노인의 우울에 관한 연구: 전기와 후기노인의 비교를 중심으로)

  • Jin-Seop Lim;Je-sun Kim
    • Journal of Industrial Convergence
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    • v.22 no.1
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    • pp.149-162
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    • 2024
  • This study is a longitudinal study of female older adults living alone, one of the most vulnerable groups in our society, to determine how their depression changes over time and what factors affect their depression. At the same time, considering that there is a large difference in age among the same older adults, this study divided the female older adults into the young-old and the old-old to see how the predictors of depression in each group differ from each other. The main findings are as follows First, depression among female older adults living alone appears to have a declining pattern over time. In the conditional model, factors affecting the initial level of the depression trajectory among women living alone were found to be associated with lower initial depression values among those living in metropolitan areas rather than non-metropolitan areas, better subjective health, and those who did not exercise. Next, we examined the factors affecting rate of change (slope) in depression among female living alone older adults and found that the higher the age, the larger the metropolitan area, the better the subjective health, the less socializing, and the more socializing, the greater the decrease in depression level. Finally, there were some differences in the pathways affecting the initial value and slope of depression among female older adults living alone between the early and late older adults. Specifically, the higher the initial level of participation in social activities, the greater the change in depression among the late older adults, while there was no significant relationship among the early older adults. In the early older adults, better initial subjective health was associated with a larger change in depression than in the late older adults. Only in the late older adults did those who regularly exercised in the early years have higher initial depression values than those who did not. Based on the results of the above analyses, suggestions were made to reduce depression among female older adults living alone.

Growth inhibition of hydrotrope-combined copper against Microcystis aeruginosa and evaluation of its toxicity (Microcystis aeruginosa에 대한 hydrotrope-combined copper의 생장억제 및 독성 평가)

  • Park, Se-Keun;Ji, Jun-Gu;Jang, Hee Jung;Kim, Yeong-Kwan;Oh, Young-Sook;Choi, Sung-Chan
    • Korean Journal of Microbiology
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    • v.51 no.1
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    • pp.7-13
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    • 2015
  • Hydrotrope-combined copper (HCC) is a copper ($Cu^{2+}$)-based algicide, which is combined with a hydrotrope that keeps copper ion in solution to improve performance. This study assessed the growth inhibition effect of HCC against Microcystis aeruginosa which is one of the most common toxic cyanobacterium in eutrophic freshwater environment. Various HCC doses, ranging from 5.5 to $550{\mu}g/L$ as $Cu^{2+}$, were applied to either BG-11 or 1/4 diluted medium with low- or high-inoculum density of M. aeruginosa. Growth inhibition was monitored based on a decrease in chlorophyll-a content in culture medium during the incubation. Results showed that HCC significantly inhibited the growth of M. aeruginosa in a dose-dependent manner. In case of 1/4 diluted BG-11 medium, HCC dose as low as $5.5{\mu}g$ $Cu^{2+}/L$ completely inhibited the production of chlorophyll-a by M. aeruginosa. It was found that HCC did not induce any significant release of microcystin-LR from M. aeruginosa. Acute toxicity of HCC was tested using Daphnia magna, and the 24-h $EC_{50}$ value was 0.30 mg/L as $Cu^{2+}$ which was much higher than the actual inhibition dose. Ames test was performed using Salmonella enterica serovar Typhimurium TA100, and HCC showed no increase in the number of revertant colonies. The result suggested that HCC does not have any mutagenic potential in the aquatic environment. In addition, no genotoxic effect of HCC was also confirmed based on the SOS ChromoTest using Escherichia coli PQ37. Therefore, HCC could be used as a relatively safe and effective pre- and post-treatment agent to control hazardous algal blooming in aquatic environments.

Extent of Subprosthetic Pannus after Aortic Valve Replacement: Changes Over Time and Relationship with Echocardiographic Findings (대동맥판막치환술 후 발생한 판막하 판누스(Pannus): 시간에 따른 변화 및 심초음파 소견)

  • Mi Yeon Park;Hyun Jung Koo;Hojin Ha;Joon-Won Kang;Dong Hyun Yang
    • Journal of the Korean Society of Radiology
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    • v.81 no.5
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    • pp.1151-1163
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    • 2020
  • Purpose This study aimed to evaluate changes of subprosthetic pannus on cardiac CT and determine its relationship to echocardiographic findings in patients with mechanical aortic valve replacement (AVR). Materials and Methods Between April 2011 and November 2017, 17 AVR patients (56.8 ± 8.9 years, 12% male) who showed pannus formation on CT and had undergone both follow-up CT and echocardiography were included. The mean interval from AVR to the date of pannus detection was 10.5 ± 7.1 years. In the initial and follow-up CT and echocardiography, the pannus extent and echocardiographic parameters were compared using paired t-tests. The relationship between the opening angle of the prosthetic valve and the pannus extent was evaluated using Pearson correlation analysis. Results The pannus extent was significantly increased on CT (p < 0.05). The peak velocity (3.9 ± 0.8 m/s vs. 4.2 ± 0.8 m/s, p = 0.03) and mean pressure gradient (36.4 ± 15.5 mm Hg vs. 42.1 ± 15.8 mm Hg, p = 0.03) were significantly increased. The mean opening angles of the mechanical aortic leaflets were slightly decreased, but there was no statistical significance (73.1 ± 8.3° vs. 69.4 ± 12.1°, p = 0.12). The opening angle of the prosthetic leaflets was inversely correlated with the pannus extent (r = -0.57, p < 0.001). Conclusion The pannus extent increases over time, increasing transvalvular peak velocity and the pressure gradient. CT can be used to evaluate the pannus extent associated with hemodynamic changes that need to be managed by surgical intervention.