• Title/Summary/Keyword: KT

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Optimization of Headspace Sampling Using Solid Phase Microextraction For Volatile Organic Acids in Different Tobacco Types

  • Lee, Jang-Mi;Lee, Jeong-Min;Son, Seong-Ae;Kwon, Young-Ju;Jang, Gi-Chui;Kim, Young-Ho
    • Journal of the Korean Society of Tobacco Science
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    • v.35 no.1
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    • pp.7-12
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    • 2013
  • A Solid-phase micro extraction(SPME) was evaluated as a tool for headspace sampling of tobacco samples. Several experimental parameters (sampling temperature, pH, and type of SPME fibers) were optimized to improve sampling efficiency in two aspects ; maximum adsorption and selective adsorption of volatile organic acids onto SPME fibers. Among four types of SPME fibers such as PDMS(Polydimethylsiloxane), PA(Polyacrylate), Car/PDMS (Carboxen/Polydimethylsiioxane) and PDMS/DVB(Polydimethylsiioxane/Divinylbenzene) which were investigated to determine the selectivity and adsorption efficiency. A variety of tobacco samples such as flue cured, burley and oriental were used in this study. The effect of these parameters was often dominated by the physical and chemical nature (volatility, polarity) of target compounds. This method allowed us to make important improvements in selectivity and sensitivity. The Car/PDMS fiber was shown to be the most efficient at extracting the 10 selected volatile organic acids. The parameters were optimized: $80^{\circ}C$ adsorption temperature, 30 min of adsorption time, $240^{\circ}C$ desorption temperature, 1 min of adsorption time.

Isolation and Development of Quantitative Determination of Torilin from the Torilis Fructus (사상자 (Torilis Fructus)의 지표성분으로 Torilin의 분리 및 함량분석법 개발)

  • Hwang, Eui-Il;Lee, Seong-Kye;So, Seung-Ho;Koo, Bon-Suk;Han, Gyeong-Ho;Kim, Na-Mi
    • Korean Journal of Pharmacognosy
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    • v.38 no.4
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    • pp.321-326
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    • 2007
  • For the propose of the quality control of Torilis Fructus, one major compound was isolated by several column chromatograpies and identified by its spectral data as torilin (8,11-dihydroxy-8-angeloyl-11-acetyl-4-guaien-3-one). A quantitative analysis of torilin using HPLC method showed that the average contents at 230 and 254 nm were $7.167{\pm}0.168mg/g\;and\;7.052{\pm}0.160mg/g$, respectively, in 18 samples collected throughout the regions of Korea and China. This method provided a tool for standardization of the commercial Torilis Fructus.

Identification of tobacco Burley species specific marker in several tobacco species by AFLP (AFLP 방법을 이용한 담배 버어리종 특이 프라이머의 개발)

  • Lee, Yung-Gi;Jung, Suk-Hun;Keum, Wan-Soo;Lee, Jeong-Heon;Lee, Cheong-Ho;Rhee, Moon-Soo
    • Journal of the Korean Society of Tobacco Science
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    • v.28 no.2
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    • pp.94-99
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    • 2006
  • AFLP(Amplified Fragment Length Polymorphism) analysis was conducted to cultivars of tobacco, Nicotiana tabacum in order to select the cultivar-specific markers. AFLP results using 12 primer sets revealed genetic diversity among 12 field grown tobacco cultivars. Polymorphic fragments amplified by PCR was purified and cloned to identify their nucleotide sequences. From the sequences of them, 40 primer sets were designed to select cultivar-specific markers. When genomic DNA isolated from tobacco were used as PCR template, a set of primers, BrSF/BrSR showed Burley-specific band patterns. The results indicate that AFLP technique used in this experiments is useful for identifying tobacco cultivars in a rapid manner.

Determination of Organic Acids in Tobacco Leaves by HPLC (HPLC를 이용한 잎담배 중 유기산 함량 분석)

  • Min, Hye-Jeong;Jang, Seok-Su;Kim, Ick-Joong;Shin, Jun-Won;Kim, Yong-Ha;Min, Young-Keun
    • Journal of the Korean Society of Tobacco Science
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    • v.28 no.2
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    • pp.130-135
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    • 2006
  • This study was carried out to improve the analytical methods for determination of organic acids in tobacco leaf by HPLC. The samples for HPLC analysis were pre-treated by means of SPE. The calibration curve for each acid was linear and $R^2$ values ranged from 0.9990 to 1.0000. The limit of detection were obtained from the calibration curves and their values were between 1.39 to $4.87{\mu}g/mL$. Recovery rates of organic acids were between 88.6 % to 98.5 %. The concentrations of organic acids among the various tobacco leaves were compared to the concentration of organic acids, were in the order oriental, burley, flue-cured tobacco. In the case of flue-cured and oriental tobacco leaves, the order of concentration of organic acids was malic acid, oxalic acid, citric acid. But in the case of burley tobacco leaves, the order of concentration of organic acids was citric acid, oxalic acid, malic acid.

Development of the Seed Filter for the enhancement of cigarette filter biodegradability (필터 생분해성 증진을 위한 종자 필터 개발 및 적용 효과)

  • Kim, Soo-Ho;Kim, Min-Kyu;Hwang, Eui-Il;Han, Young-Rim;Lee, Chang-Kuk;Yeo, Woon-Hyung
    • Journal of the Korean Society of Tobacco Science
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    • v.36 no.1
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    • pp.1-11
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    • 2014
  • Cigarette filters containing Brassica Rapa L. seeds of the dark brown and round shaped were evaluated to determine the effect of seed addition on filter degradability. The seeds with germination capability under the tar/nicotine condition in the preliminary test, were put into the active carbon part of the filter(12mm) during filter rod making by the kit. The $4{\pm}2$ pieces of the seeds were put into the opened fiber bundle of the filter tow. In order to test the germination rate of the seeds, seed filters were placed either in a petri dish or test-pot in a conditioned area ($25^{\circ}C$, 70 % RH). The seed filters were placed outdoors exposed to natural conditions with the periodic water supply. The seeds in the smoked filters showed 90 % germination rate after a month under the open air condition. No significant differences in the sensory evaluation and analysis were obtained when the control sample was compared to the same cigarettes with the seeds.

Packet data analyzing system for quality assurance of satellite network (위성망 품질보증을 위한 패킷 데이터 분석 시스템)

  • Yu Kang-Hi;Kim Jae-Kwon;Kong Kyung-Tae;Shin Dong-Houn;Lee Sang-Yun
    • 한국정보통신설비학회:학술대회논문집
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    • 2004.08a
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    • pp.298-301
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    • 2004
  • KT는 무궁화 3호 위성을 이용한 양방향 위성 통신 사업을 추진하고 있으며, 지상망을 이용하기 어려운 지역에 정부/공공, 에너지 산업, 기업 단일망 등 다양한 산업 분야를 대상으로 초고속 네트워크를 제공하고 있다. 양방향 위성 통신 서비스는 36,000km 상공의 위성 중계기와 지상 간의 통신 지연을 극복하기 위한 첨단 기술로 운용되고 있으며, 중요 데이터에 대한 위성 통신 품질보증 기능을 제공한다. 본 논문에서는 위성망의 트래픽을 수집하고 데이터를 로깅하는 위성망 트래픽 수집 도구와 수집된 데이터를 마이닝을 통해 실시간 모니터링하고 통계 정보를 생성하는 위성망 패킷 데이터 분석 도구로 이루어진 위성망 패킷 데이터 분석 시스템에 대해 논한다. 위성망 패킷 데이터 분석 시스템은 데이터 송수신 정보를 모니터링 함으로써 실시간 위성 통신 상태를 점검하고, 전송 지연 및 실패 등의 위성 통신 오류 상태를 조기 진단하고 조치할 수 있는 위성망 품질 보증 서비스를 제공한다.

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Production of Ginsenoside-Rg3 Enriched Yeast Biomass Using Ginseng Steaming Effluent (수삼 증자 시 생성되는 유출액을 이용한 ginsenoside-Rg3 강화 효모 제조)

  • Kim, Na-Mi;Lee, Seong-Kye;Cho, Hae-Hyun;So, Seung-Ho;Jang, Dong-Pil;Han, Sung-Tai;Lee, Jong-Soo
    • Journal of Ginseng Research
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    • v.33 no.3
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    • pp.183-188
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    • 2009
  • To produce ginsenoside-Rg$_3$ enriched edible yeast, ginseng steaming effluent (GSE) was used for yeast cultivation in this study. Four kinds of edible yeasts were cultured in sterilized GSE (2% w/v, pH 6.5), without any nutrient, for 48 h at 30$^{\circ}C$, and their growth and ginsenoside compositions were determined. Among the yeasts, Saccharomyces cerevisiae showed the highest growth in the GSE medium. 267.1 mg of Saccharomyces cerevisiae biomass was produced from 1 g of GSE solid and ginsenoside-Rg$_3$ contents was determined with 0.033 mg. Saccharomyces cerevisiae also showed the best overall acceptability, with a herbal and fermentative flavor and a slightly bitter taste. From these data, we conclude that Saccharomyces cerevisiae is the excellent strain for production of ginsenoside-Rg$_3$ enriched edible yeast using GSE.

System Requirement Architecture Modeling of KT-1 Export Version (수출용 KT-1 요구사항 아키텍처 모델 구축)

  • Kang, Min-Seong;Lee, Jin-Kyun;Kim, Jin-Suk;Shon, Hwan-Ick;Kang, Young-Ho;Lee, Joong-Yoon;Choi, Min-Shin;Lee, Young-Sun
    • Journal of the Korea Institute of Military Science and Technology
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    • v.9 no.1 s.24
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    • pp.135-143
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    • 2006
  • This paper describes a requirement architecture modeling for the front end of KT-1 export version requirement definition processes to construct SDS(system development specification). The requirement definition process is a highly conceptual process that is difficult to carry out. This paper focuses on how to perform the KT-1 export version requirements definition process including the integration of process, methods and tools for the front-end activity of requirements definition process. This requirement model is structured in four segments, including requirement layering, requirement categorization, life cycle stakeholder and requirement definition process using Computer-Aided Systems Engineering tool(CORE).

Predicting the Capability Curve of Cellulose Acetate Filters (셀롤로오스 아세테이트 필터의 특성곡선 예측)

  • Kim Soo-HO;Lim Sung-Jin;Kim Chung-Ryul;Shin Chang-Ho;Rhee Moon-Soo;Kim Jong-Yeol
    • Journal of the Korean Society of Tobacco Science
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    • v.27 no.2
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    • pp.163-170
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    • 2005
  • A theoretical model for predicting the capability curve of cellulose acetate filter is derived. The pressure drop is expressed as a function of the filter dimensions, the tow fiber characteristics, the filter weight, the fluid flow rate, and a filter fiber factor. Where, the filter fiber factor is affected by the distribution of the tow fibers within the filter, the relative orientations of the tow fibers, and their cross-sectional shapes. The minimum and maximum fraction of solids in capability curves determined from experimental data. Also, the filter fiber factor is expressed as a function of the filter length, tow fiber length, and tow fiber diameter. Capability curves predicted by the suggested model in this work correspond well with capability curves by experimental data.

A Study on Website Operators Privacy Breaches through Hacking Forensic Research (통신사 홈페이지 해킹을 통한 개인정보유출 사고 포렌식 연구)

  • Noh, Jung-Ho;Park, Dea-Woo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2014.05a
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    • pp.257-260
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    • 2014
  • Digital Forensics in the event of an accident, the system restore files and the only way to find evidence KT Website hacking happening now, credit card companies, and leakage of personal information by three recent spills occurred, such as Skin Food Home Up Customer Information hackers to find these crimes only means as well. This study attempted to bypass the KT website hacking attacks utilizing automated programs hacking programs, and if you try to experiment on whether any information has been disclosed and if so what home attacked forensics evidence for hackers to locate the can make a report is described.

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