Browse > Article
http://dx.doi.org/10.5142/JGR.2009.33.3.183

Production of Ginsenoside-Rg3 Enriched Yeast Biomass Using Ginseng Steaming Effluent  

Kim, Na-Mi (KT&G Central Research Institute)
Lee, Seong-Kye (KT&G Central Research Institute)
Cho, Hae-Hyun (KT&G Central Research Institute)
So, Seung-Ho (KT&G Central Research Institute)
Jang, Dong-Pil (KT&G Central Research Institute)
Han, Sung-Tai (KT&G Central Research Institute)
Lee, Jong-Soo (Department of Life Science and Genetic Engineering, Paichai University)
Publication Information
Journal of Ginseng Research / v.33, no.3, 2009 , pp. 183-188 More about this Journal
Abstract
To produce ginsenoside-Rg$_3$ enriched edible yeast, ginseng steaming effluent (GSE) was used for yeast cultivation in this study. Four kinds of edible yeasts were cultured in sterilized GSE (2% w/v, pH 6.5), without any nutrient, for 48 h at 30$^{\circ}C$, and their growth and ginsenoside compositions were determined. Among the yeasts, Saccharomyces cerevisiae showed the highest growth in the GSE medium. 267.1 mg of Saccharomyces cerevisiae biomass was produced from 1 g of GSE solid and ginsenoside-Rg$_3$ contents was determined with 0.033 mg. Saccharomyces cerevisiae also showed the best overall acceptability, with a herbal and fermentative flavor and a slightly bitter taste. From these data, we conclude that Saccharomyces cerevisiae is the excellent strain for production of ginsenoside-Rg$_3$ enriched edible yeast using GSE.
Keywords
ginsenoside-Rg$_3$ yeast; Saccharomyces cerevisiae; biomass; ginseng steaming effluent;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Lee SH, Jung BH, Kim SY, Lee EH and Chung BC. The antistress effect of ginseng total saponin and ginsenoside $\text Rg_3$ and $\text Rb_1$ evaluated by brain polyamine level under immobilization stress. Pharmacol Res. 54: 46-49 (2006)   DOI   ScienceOn
2 Kim JH, Lee BH and Lee JS. Production of ribonucleotides by autolysis of Hansenula anomala grown on ginseng steaming effluent. J Biosci Bioeng. 93: 318-321 (2002)   DOI   ScienceOn
3 Kim JH, Lee KS, Kim NM and Lee JS. Production and characterization of chitosan from ginseng steaming effluent by Mucor miehei. J Microbiol Biotechnol. 12: 760-765 (2002)   과학기술학회마을
4 Kim NM, So SH, Lee SK, Song JE, Seo DS and Lee JS. Physiological functionality and enzyme activity of biomass from Pichia anomala grown on ginseng steaming effluent. Microbiology 36: 148-151 (2008)
5 Suhajda A, Hegoczki J, Janzso B, Pais I and Vereczkey G. Preparation of selenium yeasts I. Preparation of seleniumenriched Saccharomyces cerevisiae. J Trace Elements Med Biol. 14: 43-47 (2000)   DOI   ScienceOn
6 Oh SW, Lee S, Lee HJ and Han ES. Studies on the Electrofusion applied to the yeast to produce high quality of organic germanium. Korean J Food Sci Technol. 38: 712-716 (2006)
7 Demirci A, Pometto AL and Cox DJ. Enhanced organically bound selenium yeast production by fed-batch fermentation. J Agric Food Chem. 47: 2496-2500 (1999)   DOI   ScienceOn
8 Lynn MR and Geoffrey MG. Mutants of Saccharomyces cerevisiae defective in vacuolar function a role for the vacuole in toxic metal ion detoxification. Microbiol Lett. 152: 293-298 (1997)   DOI   ScienceOn
9 Kim JH, Lee DH, Jeong SC, Chung KS and Lee JS. Characterization of antihypertensive angiotensine Ⅰ converting enzyme inhibitor from Saccharomyces cerevisiae. J Microbiol Biotechnol. 14: 1318-1323 (2004)
10 Lee JO, Kim Y O, Shin DH, Shin JH and Kim EK. Production of selenium peptide by autolysis of Saccharomyces cerevisiae. J Microbiol Biotechnol. 16: 1041-1046 (2006)   과학기술학회마을
11 Ali D and Anthony L P. Production of organically bound selenium yeast by continuous fermentation. J Agric Food Chem. 47: 2491-2495 (1999)   DOI   ScienceOn
12 Kown SW, Han SB, Park IH, Kim JM, Park MK and Park J H. Liquid chromatographic determination of less polar ginsenosides in processed ginseng. J Chromatogr A 921: 335-339 (2001)   DOI   ScienceOn
13 Kim ND, Kang SY, Park JH and Schini-kerth VB. Ginsenoside-$\text Rg_3$ mediate endothelium-dependent relaxation in response to ginsenosides in rat aorta : role of $\text K^+$ channels. Eur J Pharmacol. 367: 41-49 (1999)   DOI   ScienceOn
14 Kim WY, Kim JM, Han SB, Lee SK, Kim ND, Park MK, Kim CK and Park JH. Steaming of ginseng at high temperature enhances biological activity. J Nat Prod. 63: 1702-1704 (2000)   DOI   ScienceOn
15 Keum YS, Han SS, Chun KS, Park, KK, Park J H, Lee SK and Surh YJ. Inhibitory effects of the ginsenoside-$\text Rg_3$ on phorbol ester-induced cyclooxygenase-2 expression, NFkappaB activation and tumor promotion. Mutat Res. 323: 75-85 (2003)   DOI   ScienceOn
16 Park S, Kim EH, Na HK and Surh YJ. KG-135 inhibits COX-2 expression by blocking the activation of JNK and AP-1 in phorbol ester-stimulated human breast epithelial cells. Ann NY Acd Sci. 1095: 545-553 (2007)   DOI   ScienceOn
17 Chasteen TG and Bentley R. Biomethylation of selenium and tellurium. microorganisms and plants. Chem Rev. 103: 1-25 (2003)   DOI   ScienceOn
18 Choi MH, Ji GE, Koh KH, Ryn YW, Jo DH and Park YH. Use of waste Chinese cabbage as a substrate for yeast biomass production. Bioresource Technol. 83: 251-253 (2002)   DOI   ScienceOn
19 Choi MH and Park YH. Growth of Pichia guilliermondii A9, an osmotolerant yeast, in waste brine generated from Kimchi production. Bioresource Technol. 70: 231-236 (1999)   DOI   ScienceOn
20 Chanda S and Chakrabatri S. Plant origin liquid waste : a resource for single cell protein production by yeast. 57: 51-54 (1996)   DOI   ScienceOn
21 Chen F, Eckman EA and Eckman CB. Reductions in levels of the Alzheimer's amyloid beta peptide after oral administration of ginsenoside. FASEB J. 20: 1269-1271 (2006)   DOI   ScienceOn
22 Kim JH, Lee JH, Jeong SM, Lee BH, Yoon IS, Lee JH, Choi SH, Kim DH, Park TK, Kim BK and Nah SY. Stereospecific effects of ginsenoside-$\text Rg_3$ epimers on swine coronary artery contractions. Biol Pharm Bull. 29: 365-370 (2006)   DOI   ScienceOn
23 Kim YC, Kim SR, Markelonis GL and Oh TH. Ginsenoside $\text Rb_1$ and $\text Rg_3$ protect cultured rat cortical cells from glutamate-induced nerodegeneration. J Neurosci Res. 53: 426-432 (1998)   DOI   ScienceOn
24 Tian JF, Geng M, Jiang Y, Yang J, Jiang W, Wang C and Lin K. Neuroprotective effect of 20(s)-ginsenoside $\text Rg_3$ on cerebral ischemia in rats. Neurosci Lett. 374: 92-99 (2005)   DOI   ScienceOn
25 Lee SH, Ahn SD, Rho SY and Shou TU. A study preparation and binding properties of germanium-fortified yeast. J Korean Soc Appl Biol Chem. 48: 382-387 (2005)
26 Hang YD, Woodams EE and Hang LE. Utilization of corn silage juice by Klyuveromyces marxianus. Bioresource Technol. 86: 305-307 (2003)   DOI   ScienceOn
27 Hongfei Y, Zhiang C, Zhenxin G and Yongbin H. Optimization of natural fermentative medium for seleniumenriched yeast by D-optimal mixture design. LWT-Food Sci. Technol. 42: 327-331 (2009)   DOI   ScienceOn