• Journal of the Korean Chemical Society
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• v.63 no.5
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• pp.342-345
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• 2019

The standards for the contents of glyphosate and glufosinate in foods are specific and well categorized. However, the standard of content in animal feeds is relatively inadequate and the classification is insufficient. There is also constant debate about the risk of glyphosate and glufosinate to human health, but the risk to animals has not been well studied. In this study, we established an analytical method in feeds that is estimated to be the path for animals to ingest glyphosate. The solvent extraction was carried out using 25% methanol. After centrifugation, samples were purified using solid phase extraction (SPE) and quantitatively analysed using LC-MS/MS after concentrated. Assessment of validation was conducted through detection limits, accuracy, and precision tests. The detection limits for the established method were 1.8 of ${\mu}g/kg$ of glufosinate and $2.4{\mu}g/kg$ of glyphosate. Accuracy was ranged from 94.4% to 103.4% and precision was range from 1.5% to 7.2%. Glufosinate was detected in one sample ($ND{\sim}8.8{\mu}g/kg$) and glyphosate was detected in all but one sample ($ND{\sim}337.0{\mu}g/kg$) by applying the analytical method to animal feeds (n=13).

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.1
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• pp.103-108
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• 2015

Curcumin is widely used as a traditional medicine. This work was aimed to investigate its possible protective effect against chemically induced hepatocellular carcinoma (HCC) in rats. Fifty male albino rats were divided into five groups (n=10, each). The control group received a single dose of normal saline, the diethylnitrosamine (DENA) group received a single intra-peritoneal dose at 200mg/kg body weight, and the 3rd, 4th and 5th groups were given DENA and daily administrated curcunine (CUR) via intra-gastric intubation in doses of 300, 200 and 100 mg/kg b.wt. respectively for 20 weeks. Serum, and liver samples were used for determination of alpha feto-protein (AFP), interleukin-2 (IL-2), interleukine-6 (IL-6), serum liver enzymes (AST, ALT, ALP and GGT) levels as well the activities and gene expression of glutathione peroxidise (GPx), glutathione reductase (GR), catalase (CAT) and super oxide dismutase (SOD). Curcumin significantly lowered the serum levels of AFP, IL-2 and IL-6, ALT, ALT, and malondialdehyde (MDA) as well gene expression of IL-2 and IL-6. In contrast it increased the gene expression and activities of Gpx, GRD, CAT and SOD. The protective effect of CUR against DEN-induced hepatocarcinogenesis in albino rats was proven.

• Preventive Nutrition and Food Science
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• v.1 no.1
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• pp.1-5
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• 1996

Rapid analytical method was investigated to determine precursors of N-nitrosamine such an nitrite and nitrate in squid(Illex illecebrosus and Sepiell maindroni), codfish(Gadus marcrocephalus)and flatfish(Paralichthys olivaceus) by ion chromatography(IC) and colorimetric methods. Recoveries of nitrite and nitrate in fish tissues were 89~98.7% and 94.1~99.8% for IC, and 98.4~103.7% and 67.7~102.2% for colori-metric method, respectively. Using IC, nitrite was not detected and nitrate was 0.89~1.23mg/kg, while using colorimetric method, nitrite and nitrate were NO~0.08mg/kg and 0.3~0.42mg/kg, respectively. Therefore, the IC method showed better recoveries, and was applicable to extract nitrite and nitrate simultaneously, and is simpler compared with colorimetric method in analyzing nitrite and nitrate from fish tissues.

• The Korean Journal of Pesticide Science
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• v.18 no.1
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• pp.1-7
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• 2014

The present study was performed to investigate the pre-harvest residue limit (PHRL) of pesticides namely, metalaxyl-M and flusilazole in oriental melon, and to identify the biological half-life and characteristics of their residues. In this study, pesticides were sprayed once as single spray and double spray on oriental melon. The oriental melon samples were collected at 0, 1, 2, 3, 5, 7, 9 and 11 days before harvest and samples were extracted with QuEChERS method. The residues of both the pesticides were quantified using GC/NPD and LC/MS/MS. The limit of detection was found to be 0.02 mg/kg and 0.01 mg/kg and their recoveries were greater than 95% (95.7% ~ 103.2% for metalaxyl-M and 100.2% ~ 106.8% for flusilazole) for both pesticides. The biological half-lives of both metalaxyl-M and flusilazole were 12 days at single and double spray, respectively. The PHRL of metalaxyl-M and flusilazole was found 1.0 mg/kg and 0.3 mg/kg, respectively for 10 days before harvest. The results of the present study shows the residual level of both the pesticides metalaxyl-M and flusilazole in oriental melon were less than their maximum residual limits.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.3
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• pp.308-317
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• 1996

These experiments were conducted to investigate variation of physical characteristics of the culm related to lodging resistance by nitrogen levels under direct seeding on flooded paddy surface. The number of seedling per m$^2$ were from 103 to 110 plants, and seedling ratios were ranged from 66.7% to 71.2%. The lodging occurrence were increased in order to the nitrogen levels 15 kg, 10 kg, 5 kg /10a, and the lodging resistant varieties ; Dongjinbyeo and Cheongmyungbyeo showed less values of field lodging than those of lodging susceptible varieties ; Daecheongbyeo, Palgongbyeo and H waseongbyeo. The lodging resistance was decreased in semidwarf varieties compare with long culm varieties, but Dongjinbyeo, long culm variety has lodging resistance. The occurrence of lodging decreased with lower values in top moment, but with higher values in the breaking moment with leaf sheath. The root dry weight positively correlated with weight of culm base, but modulus of section was negatively correlated with bending curvature, respectively.

• Journal of Pharmaceutical Investigation
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• v.14 no.2
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• pp.92-103
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• 1984

The action of debrisoquine on renal function in rabbits was studied. 1. When debrisoquine was given into ear vein, it did not affect on renal functin with smaller doses of 0.1 or 0.3mg/kg, while with higher dose of 1.0mg/kg it elicited the significant decrease of urine flow, renal plasma flow and glomerular filtration rate, and the increase of filtration fraction, and at the same time sodium excreted in urine, FENa (fractional excretion of sodium) and osmolar clearance were significantly decreased, and then it exhibited the increase of $K^+/Na^+$ ratio and no changes of $T^cH_2O$. 2. Debrisoquine (1.0mg/kg), when injected repeatedly into a vein, produced a more marked decrease of urine flow. 3. Debrisoquine induced-antidiuretic action was not affected by pretreatment with phentolamine (2mg/kg, i.v.), alpha-sympathetic blocking agent. 4. Debrisoquine given intracerebroventricularly did not produce a significant change on renal function in dose of 0.1mg/kg. These results suggest that debrisoquine produce the antidiuretic effect in rabbit, and the mechanism of its action is due to dual actions that are the decrease of hemodynamic effect and the facilitation of reabsorption of sodium in renal tubules.

• Animal Bioscience
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• v.34 no.10
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• pp.1623-1631
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• 2021

Objective: This study aimed to validate and evaluate the dry matter (DM) intake prediction model of the Korean feeding standards for dairy cattle (KFSD). Methods: The KFSD DM intake (DMI) model was developed using a database containing the data from the Journal of Dairy Science from 2006 to 2011 (1,065 observations 287 studies). The development (458 observations from 103 studies) and evaluation databases (168 observations from 74 studies) were constructed from the database. The body weight (kg; BW), metabolic BW (BW^0.75, MBW), 4% fat-corrected milk (FCM), forage as a percentage of dietary DM, and the dietary content of nutrients (% DM) were chosen as possible explanatory variables. A random coefficient model with the study as a random variable and a linear model without the random effect was used to select model variables and estimate parameters, respectively, during the model development. The best-fit equation was compared to published equations, and sensitivity analysis of the prediction equation was conducted. The KFSD model was also evaluated using in vivo feeding trial data. Results: The KFSD DMI equation is 4.103 (±2.994)+0.112 (±0.022)×MBW+0.284 (±0.020)×FCM-0.119 (±0.028)×neutral detergent fiber (NDF), explaining 47% of the variation in the evaluation dataset with no mean nor slope bias (p>0.05). The root mean square prediction error was 2.70 kg/d, best among the tested equations. The sensitivity analysis showed that the model is the most sensitive to FCM, followed by MBW and NDF. With the in vivo data, the KFSD equation showed slightly higher precision (R² = 0.39) than the NRC equation (R² = 0.37), with a mean bias of 1.19 kg and no slope bias (p>0.05). Conclusion: The KFSD DMI model is suitable for predicting the DMI of lactating dairy cows in practical situations in Korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.2
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• pp.103-107
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• 2014

Tall fescue (Festuca arundinacea Schreb.) is one of cool-season adapted perennial grass species and has been cultivated worldwide as a important forage and lawn grass. In this study, we evaluated the seed production of new tall fescue cultivar, 'Purum', which was cultivated in Korea. Agronomic aspects of tall fescue were determined using seed production and forage nutritive value of straw at the National Institute of Animal Science, RDA from 2012 to 2013. The heading date according to the seeding time displayed 2~3 days differences. The harvested seed yields were the largest (1,711 kg/ha) when seeding date at September $17^{th}$, whereas it decreased when planted ten days earlier or later than at that time. The thousand-grain weight of gathering seeds was 2 g, and the germination rate was 80 to 83% showing no significant differences among the different seeding times. The yield of fresh weight and dry matter were the largest (63,556 kg/ha and 16,926 kg/ha, respectively) when planted at September $17^{th}$. The dry matter yield was gradually decreased with delaying seeding time as 10 days period. However, nutritive values of straw did not showed significant differences.

• Journal of Climate Change Research
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• v.4 no.1
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• pp.27-39
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• 2013

In this study, the anthracite coal being used as fuel in Korea were classified into different types. These types include the domestically produced anthracite, imported anthracite used as raw material, and imported anthracite used as fuel. Each of the calorific values and greenhouse gas emission factors were calculated. The calculation of greenhouse gas emission factors resulted in the domestically produced anthracite as $111,477{\pm}4,508kg\;CO_2/TJ$, the imported anthracite used as raw material as $108,358{\pm}4,033kg\;CO_2/TJ$, and anthracite used as fuel was displayed as $103,927{\pm}8,367kg\;CO_2/TJ$. Additionally, the amount of greenhouse gas emission based on these calculated emission factors was displayed as $6,216,942ton\;CO_2$, which resulted as 12.7% lower than the green house gas emission amount which was calculated without distinguishing anthracite coal in details. Therefore, collecting activity data through a detailed classification of anthracites facilitate a more accurate calculation of greenhouse gas emission amount compared to collecting activity data through combination. Furthermore, since the anthracite coal used domestically possesses characteristics differing from the anthracite coal proposed by the IPCC, anthracite coal should be classified for each purpose and calculated for the improvement of the national greenhouse gas inventory.

• Korean Journal of Applied Biomechanics
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• v.24 no.4
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• pp.417-423
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• 2014

The purpose of this study was to compare two saddle height determination methods by the effectiveness of pedal reaction force. Ten male subjects (age: $24.0{\pm}2.4years$, height: $175.1{\pm}5.4cm$, weight: $69.3{\pm}11.1kg$, inseam: $77.8{\pm}4.5cm$) participated in three minutes, 60 rpm cycle pedaling tests with the same load and cadence. Subject's saddle height was determined by $25^{\circ}$ knee flexion angle (K25) when the pedal crank was at the 6 o'clock position (knee angle method) and 97% (T97), 100% (T100), 103% (T103) of trochanter height (trochanteric method). The RF (resultant force), EF (effective force), and IE (index of effectiveness) were compared by measuring 3D motion and 3-axis pedal reaction force data during 4 pedaling phases (phase1: $330^{\circ}-30^{\circ}$, phase2: $30^{\circ}-150^{\circ}$, phase3: $150^{\circ}-210$, phase4: $210^{\circ}-330^{\circ}$). Results showed that there were significant differences in EF at phase1 between T97 and K25, in EF at phase4 between T100 and T103, in IE at total phase between T97 and K25, between T100 and T103, in IE at phase1 & phase2 between T97 and K25. There was higher IE in the K25 than any other saddle heights, which means that K25 was better pedaling effectiveness than the trochanteric method. Therefore it was suggested the saddle height as 103.7% of trochanter height that converted from K25.