• International Journal of Stem Cells
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• v.16 no.1
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• pp.52-65
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• 2023

Background and Objectives: Epithelial-Mesenchymal transition (EMT) is one of the origins of myofibroblasts in renal interstitial fibrosis. Mesenchymal stem cells (MSCs) alleviating EMT has been proved, but the concrete mechanism is unclear. To explore the mechanism, serum-free MSCs conditioned medium (SF-MSCs-CM) was used to treat rat renal tubular epithelial cells (NRK-52E) fibrosis induced by transforming growth factor-β1 (TGF-β1) which ameliorated EMT. Methods and Results: Galectin-3 knockdown (Gal-3 KD) and overexpression (Gal-3 OE) lentiviral vectors were established and transfected into NRK-52E. NRK-52E fibrosis model was induced by TGF-β1 and treated with the SF-MSCs-CM for 24 h after modelling. Fibrosis and autophagy related indexes were detected by western blot and immunocytochemistry. In model group, the expressions of α-smooth muscle actin (α-SMA), fibronectin (FN), Galectin-3, Snail, Kim-1, and the ratios of P-Akt/Akt, P-GSK3β/GSK3β, P-PI3K/PI3K, P-mTOR/mTOR, TIMP1/MMP9, and LC3B-II/I were obviously increased, and E-Cadherin (E-cad) and P62 decreased significantly compared with control group. SF-MSCs-CM showed an opposite trend after treatment compared with model group. Whether in Gal-3 KD or Gal-3 OE NRK-52E cells, SF-MSCs-CM also showed similar trends. However, the effects of anti-fibrosis and enhanced autophagy in Gal-3 KD cells were more obvious than those in Gal-3 OE cells. Conclusions: SF-MSCs-CM probably alleviated the EMT via inhibiting Galectin-3/Akt/GSK3β/Snail pathway. Meanwhile, Gal-3 KD possibly enhanced autophagy via inhibiting Galectin-3/Akt/mTOR pathway, which synergistically ameliorated renal fibrosis. Targeting galectin-3 may be a potential target for the treatment of renal fibrosis.

• Proceedings of the Korean Information Science Society Conference
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• 2007.06a
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• pp.87-88
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• 2007

• Proceedings of the Korea Crystallographic Association Conference
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• 2000.11a
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• pp.10-10
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• 2000

• Journal of Distribution Science
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• v.12 no.7
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• pp.53-61
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• 2014

Purpose - This study focuses on "large-scale marts," which is a typical discount-based retail channel (hereinafter, DRC), and provides practical managerial implications by applying the KD-SQS service quality factor based on customers' experiential perspective by developing and applying existing service measures. Research design, data, and methodology - The research subjects include adults who have experienced "large-scale marts." The research involved SPSS 20.0 and AMOS 19.0 packages; path analysis is used to analyze structural relationships. Results - First, physical aspects, human interaction, and additional convenience aspects of service quality have statistically significant influence on service value. Second, physical aspects, human interaction, and policy have statistically influence on store loyalty. Third, service value influences store loyalty. Fourth, service guarantee adjusts the relationship between service quality, service value, and store loyalty in terms of human interaction and policy. Conclusion - Among service quality measures, improving service value through personal service needs to be prioritized, while we need to develop different methods for the service guarantee system to effectively influence service value and store loyalty.

• Ocean Systems Engineering
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• v.5 no.4
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• pp.261-277
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• 2015

Internal solitary waves occur due to density stratification and are nonlinear in nature. These waves have been observed in many parts of the world including the South China Sea, Andaman Sea and Sulu Sea. Their effect on floating systems has been an emerging field of interest and recent offshore developments in the South China Sea where several offshore oil and gas discoveries are located have confirmed adverse effects including large platform motions and riser system damage. A valid numerical model conforming to the physics of internal waves is implemented in this paper and the effect on a spar platform is studied. The physics of internal waves is modeled by the Korteweg-de Vries (KdV) equation, which has a general solution involving Jacobian elliptical functions. The effects of vertical density stratification are captured by solving the Taylor Goldstein equation. Fully coupled time domain analyses are conducted to estimate the effect of internal waves on a typical truss spar, which is configured to South China Sea development requirements and environmental conditions. The hull, moorings and risers are considered as an integrated system and the platform global motions are analyzed. The study could be useful for future guidance and development of offshore systems in the South China Sea and other areas where the internal wave phenomenon is prominent.

• Applied Biological Chemistry
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• v.35 no.3
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• pp.191-195
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• 1992

In order to elucidate the plant-microorganism relationship, we purified an ethylene-inducible, basic 30 KD endochitinase from bean leaves and studied its antifungal activity by a hyphal extension-inhibition assay. The purified chitinase was effective in the inhibition of hyphal growth of Aspergillus fumigatus, Botrytis cinerea, Fusarium oxysporum, Rhizoctonia solani, while microbial chitinases of Serratia marcescens and Streptomyces griceus, egg white lysozyme and papya protease didn't affect hyphal growth of the fungi. The chitinase degraded the cell walls of Micrococcus lysodeikticus, suggesting the lysozyme activity of the chitinase. We discussed the implication of the bifunctional chitinase/lysozyme activities of the protein with hydrolysis of chitin in the rapidly extending hyphae of the fungi.

• Journal of Radiation Protection and Research
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• v.10 no.1
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• pp.3-13
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• 1985

The sorption behavior of some typical fission products such as Cs-137, long-lived radionuclide; Ce-144, rare-earth element; and Co-60, corrosion product on zeolite A, zeolite F-9 (faujasite) and amorphous zeolite was determined with the salt concentrations, 0.01 M- to 2.0 M- nitric acid and ammonium nitrate, and the shaking time, 15 minutes interval from 15 minute to 90 minute. Kd values were obtained through the batch experiment. In conclusion, the optimal conditions for isolation and removal of the typical radionuclides are as following: zeolite, amorphous zeolite; concentration, $0.01\;M-HNO_3\;and\;0.1\;M-NH_4NO_3$; pH4; shaking time, one hour; the most effective species, Cs-137.

• BMB Reports
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• v.45 no.9
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• pp.521-525
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• 2012

In addition to its pivotal role in neuronal survival, PI3K/Akt signaling is integral to neuronal differentiation and neurite outgrowth. However, the exact role of Akt in neuronal differentiation is still controversial. Here, we found that nuclear expression of CA-Akt resulted in unusual rapid neurite outgrowth and overexpression of KD-Akt caused multiple dendrite growth without specific axon elongation. Moreover, microarray data revealed that the expression of FOXQ1 expression was about 10-fold higher in cells with nuclear, active Akt than in control cells. Quantitative real-time PCR analysis showed that mRNA levels were upregulated in NLS-CA-Akt cells as compared to KD or EV cells. Furthermore, our FACS analysis demonstrated that overexpression of NLS-CA-Akt accumulate cells in the G1 phase within 24 h, fitting with the rapid sprouting of neuritis. Thus, our data implied that at least in this early time frame, the overexpression of nuclear, active Akt forced cells into neurite development through probably FOXQ1regulation.

• Journal of applied mathematics & informatics
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• v.29 no.5_6
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• pp.1583-1602
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• 2011

A special system of partial differential equations (PDEs) occur in a natural way while studying a class of irrotational inviscid fluid flow problems involving infinite channels. Certain aspects of solutions of such PDEs are analyzed in the context of flow problems involving multiple layers of fluids of different constant densities in a channel associated with arbitrary bottom topography. The whole analysis is divided into two parts-part A and part B. In part A the linearized theory is employed along with the standard Fourier analysis to understand such flow problems and physical quantities of interest are derived analytically. In part B, the same set of problems handled in part A are examined in the light of a weakly non-linear theory involving perturbation in terms of a small parameter and it is shown that the original problems can be cast into KdV type of nonlinear PDEs involving the bottom topography occurring in one of the coefficients of these equations. Special cases of bottom topography are worked out in detail and expressions for quantities of physical importance are derived.

• Proceedings of the KSME Conference
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• 2004.11a
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• pp.1617-1622
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• 2004

Two-phase flow of liquid and gas through pipe lines are frequently encountered in nuclear power plant or industrial facility. Pressure waves which can be generated by a valve operation or any other cause in pipe lines propagate through the two-phase flow, often leading to severe noise and vibration problems or fatigue failure of pipe line system. It is of practical importance to predict the propagation characteristics of the pressure waves for the safety design for the pipe line. In the present study, a theoretical analysis is performed to understand the propagation characteristics of a weak shock wave in a bubbly flow. A wave equation is developed using a small perturbation method to analyze the weak shock wave through a bubbly flow with comparably low void fractions. It is known that the elasticity of pipe and void fraction significantly affect the propagation speed of shock wave, but the frequency of relaxation oscillation which is generated behind the shock wave is not strongly influenced by the elasticity of pipe. The present analytical results are in close agreement with existing experimental data.