• Journal of Animal Environmental Science
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• v.10 no.2
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• pp.119-126
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• 2004

The protein in the extracts from the skins of pineapple and kiwi and the optimal conditions to hydrolyze blood, egg and gluten meals with them were investigated. Protein analysis by SDS-polyacylamide gel electrophoresis showed one protein band with 22 kd molecular weight in the pineapple skin extract, and Hve protein bands with 27 kd, 22.5 kd, 22 kd, 19 kd, and 14.4 kd molecular weight in the kiwi skin extract. The 22 kd protein in the pineapple skin extract is assumed to be bromelain, and the 27 kd protein in the kiwi skin extract is assumed to be actinidin, both are pretense. The optimal conditions for hydrolysis of blood, egg, and gluten meals we: 6-24 hours in time, $60^{\circ}C$ in temperature, and pH 4-pH 7.

• The Korean Journal of Mycology
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• v.26 no.4 s.87
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• pp.551-561
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• 1998

Plasma membrane from the fungal cells (Aspergillus phoenicis, Rhizopus acidus, Candida albicans) treated with sodium benzoate (S.B), potassium sorbate (P.S) and calcium propionate (C.P) during the cultivation were separated. The contents and patterns of plasma membrane proteins compared with those of the control. The growth of A. phoenicis was decreased by the average 64.0% in the S.B treatment. That of R. acidus was inhibited by the average 69.0% in the P.S treatment. Also, That of C. albicans was showed the deminution of the average 59.5% in the S.B treatment. The contents of protein involved in the plasma membrane of the each fungal cells were inhibited the average 41.0%, 41.7% and 59.5% in the S.B treatment, respectively. In case of A. phoenicis, the changes in the protein pattern involved in the plasma membraneshowed the aspect similar to the control on the 1st day and 2nd day of cultivation in the treatment group, but $116\;KD{\sim}97\;KD$ band almost disappeared in the 5th day of cultivation, and $45\;KD{\sim}29\;KD$ band was uncleared through the cultivation. In S.B treatment group R. acidus was showed the loss of $116\;KD{\sim}97\;KD$ band from the middle stage of cultivation and P.S, and C.P treatment group were started the loss at the early stage and completely lost at the 36 hours of cultivation. In C. albicans, $116\;KD{\sim}97\;KD$ band were started the loss at the early stage to compare with the control and $66\;KD{\sim}45\;KD$ band were dimmed at the 96 hours of cultivation. Especially, the C.P treatment group were perfectly lost at the 96 hours of cultivation.

• The Korean Journal of Zoology
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• v.32 no.4
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• pp.412-419
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• 1989

Monoclonal antibodies (MAbs) reacting with the plasmalemma of Amoeba proteus were produced. Specificity of the 3 MAbs was determined by transfer blotting of the SDS polvacryfamide gel. AMS antibody reacted with the mucopolysaccharide bands of the spacer gel, 220 KD and 50 KD proteins of the resolving gel. The maior glycoprotein bands (175 KD, 165 KD) and 50 KD protein of the plasmalemma were recognized by AUG antibody. A third, AMP antibody reacted with the 50 KD protein only. In immunofluorescence microscopy of the enzyme treated cells, the antigens of these MAbs were sensitive to proteases, but not sensitive to neuraminidase. In the assay of cell to substratum attachment after binding with the antibody, AMG and AMP antibodies exerted no effect, but AMS hindered the attachment and cell spreading. Thus the effective components of the plasmalemma in cell to substratum attachment appear to be the mucopolysaccharides and 220 KD protein. The membranes of latex particle infested phagosomes did not show any distinction from the plasmalemma in fluorescence microscopy. Phagosome membranes of amoebae appear to be derived from the plasma membrane without selection in terms of the antigen composition. Amoeba Proteus의 세포막과 반응하는 단세포군 항체를 생산하였다. SDS polyacrylamide gel을 transfer blotting하여 이들 항체의 반응 특이성을 조사해 본 결과 AMS 단항체는 PAS로 염색되는 spacer gel의 mucopolysaccharide 린드, resolving gel의 220 KD 및 50 KD 단백질과 반응하였으며, 세포막의 주요 당단백질인 175 KD 및 165 KD 빈드와 50 KD 단백질은 AMG 단항체에 의해서 인지되었다. 그리고 AMP단항체는 공통인 50 KD 단백질과 특이하게 반응하였다. 효소처리한 아메바의 면역형광칠미경적 조사에서 이들 항체에 대한 항원분자들은 모두 단백질분해효소에 민감하였으며 neuraminidase에 대해서는 변화가 없었다. 이들 항체를 결합시킨 아메바의 용기표면 부착 가능성을 분석한 결과 AMP 및 AMG 단항체는 아무런 영향을 미치지 못하였으며 AMS 단항체는 세포의 용기표면 부착 및 세포의 펴짐을 저해하였다. 따라서 아메바의 용기표면 부착은 mucopolysaccharide 및 220 KD 단백질에 의해서 매게되는 것으로 나타났다. 그리고 latex particle을 담고 있는 식포막은 면역형광형미경적 조사에서 세포막과 차이가 없었다. 따라서 겐포막은 항원 조성에 있어서 비 선택적으로 세포막에서 유도되는 것으로 나타났다.

• Korean Journal of Microbiology
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• v.42 no.2
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• pp.103-110
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• 2006

T-RFLP analysis and clone sequencing analysis based on bacterial 16S rDNA were conducted to assess bacterial community structure and diversity in Zoysia japonica soil treated with liquid fertilizer containing amino acids(LFcAA) after spray with herbicide. The results of T-RFLP (terminal restriction fragment length poly-morphism) analysis using restriction enzyme Hae III showed that the T-RFs of various size appeared evenly in the 32 clones of KD3 and 38 clones of KD4 respectively that had been treated with liquid fertilizer containing amino acid(LFcAA) compared to 23 clones of KD2 hat had not been treated with LFcAA. The microbial com- munity structure in KD2 appeared less diverse than those in KD3 and KD4. Analysis of partial sequences for 110 clones from KDI (control), KD2 (non-treated), KD3 (LFcAA 1X), KD4 (LFcAA 2X), respectively, revealed that most bacteria were related with uncultured bacteria in a 16S rDNA sequence similarity range of 91-99% through blast search. Otherwise, the other clones were members of proteobacteria, Acidobacteria, Act-inobacteria, Sphingobacteria and Planctomyces groups. Especially in KD4, members of Alpha Proteobacteria, Rhizobiales, Sphigomonadales, Caulobacterales, Gamma Proteobacteria, the genus Pseudomonas, Betapro-teobacteria, Nitrosomonadales and genus Nitrosospira appeared to be dominant. In addition, Acidobacteria group, Actinobacteria group, Planctomycetacia and Sphingobacteria were also shown. The microbial com-munity structure in Z. japonica soil sprayed with herbicide was affected by LFcAA.

• Korean journal of applied entomology
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• v.33 no.3
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• pp.178-183
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• 1994

The prolein components of each crystal toxin of Bacillus thuringiensis kurstaki and alzawai were separated by SDS-PAGE. The major clqistal proteins from both shins were composed of paiypeptides having molecular weights of 130 kd and 64 kd. Digestive mixture of both. toxins with t~ypsin and gut juices shared 62 kd polypeptide which may be major actwe toxh. However, aizawal produced much less amount of 62 kd polypeptide than kurstaki did. On ingestion with Bt &-endotoxin, larvae of Hyphantria cunea developed 45 kd stress protein in the several tissues including fat body, which was induced by heat and cold shock.

• Korean Journal of Logic
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• v.8 no.2
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• pp.85-107
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• 2005

Kleene first investigated a three-valued system which follows the evaluations of the Lukasiewicz infinite-valued logic ${\L}C$ with respect to negation, conjunction, and disjunction, and treats $\rightarrow$ as material-like implication in the sense that A $\rightarrow$ B is defined as ${\sim}A{\vee}B$ in its evaluation. Diense and Rescher extended it to many-valued logic and infinite-valued logic, respectively. This paper investigates a variant of the infinite-valued Kleene-Diense logic KD, which we shall call strong Kleene-Diense logic (sKD): sKD has the same evaluations as KD except that sKD takes a variant of Kleene-Diense implication. Following the idea of Dunn [2], we provide algebraic completeness for sKD together with its deduction theorem.

• Journal of applied mathematics & informatics
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• v.29 no.1_2
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• pp.351-367
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• 2011

In this article, we construct exact traveling wave solutions for nonlinear PDEs in mathematical physics via the (1+1)- dimensional combined Korteweg- de Vries and modified Korteweg- de Vries (KdV-mKdV) equation, the (1+1)- dimensional compouned Korteweg- de Vries Burgers (KdVB) equation, the (2+1)- dimensional cubic Klien- Gordon (cKG) equation, the Generalized Zakharov- Kuznetsov- Bonjanmin- Bona Mahony (GZK-BBM) equation and the modified Korteweg- de Vries - Zakharov- Kuznetsov (mKdV-ZK) equation, by using the (($\frac{G'}{G}$) -expansion method combined with the Riccati equation, where G = $G({\xi})$ satisfies the Riccati equation $G'({\xi})=A+BG^2$ and A, B are arbitrary constants.

• Animal cells and systems
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• v.5 no.3
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• pp.199-203
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• 2001

Sperminogen was purified from the acid extracts of boar spermatozoa and partial peptide sequence of the 34-36 kd sperminogen was determined. Acid extracts of boar spermatozoa was gel-filtered through Sephadex G-75, and the 34-36 kd sperminogen was purified by preparative SDS-PAGE. The sperminogen bands were sliced out, and 34-36 kd sperminogen were eluted from the gel fragments and was subjected to peptide sequencing. Since the amino termini were blocked for Edman degradation method, internal amino acid sequences of the eluted 34-36 kd sperminogen were obtained from CNBr-digested peptides of sperminogen. Among several bands resolved on tricine SDS-PAGE, 14, 22 and 26 kd peptides were subjected to peptide sequencing. The ana1yzed amino acid sequences of the 26 and 22 kd peptides showed high homologies with that of the zona pellucida binding protein, Sp38, and the analyzed amino acid sequence of the 14 kd peptide showed neither sequence homology nor similarity with any known proteins.

• The Korean Journal of Zoology
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• v.35 no.2
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• pp.125-135
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• 1992

참개구리의 난자형성 단계에 따른 난황막의 구조적 변화와 막단백질의 변화에 대하여 연구함으로써 난자형성에 따른 난탈막의 기능적 분화의 가능성을 알아보고자 하T:다. 난황막의 구조적 변화는 난자형성에 따른 미세음모의 수와 모양의 변화로 확인되었다. 그 수가 초기에는 적으나 중기에 증가하고 알기에 다시 감소하며, 그 모양도 처음에는 킬고 가늘지만, 나중에는 짧고 굵어진다. 막단백질은 wheat germ agglutinin에 대해서만 특이하게 반응을 보이는 당단백질로서 작은 난모세포(직경 100-800 urn)와 큰 난모세포(직경 1500 urn)에서 다르게 나타난다. 분자량은 106 KD, 60 KD, 순 KD과 같이 어느 단계에서나 공통적으로 나타나는 단백질이 존재하는 반면에 특히 작은 난모세포의 난황막에서만 찾아 볼 수 있는 130 KD, 125 KD, 90 KD, 28 KD, 26KD과 같은 단겐 특이적으로 나타나는 막단백질도 있다. 이와 같은 결과로 미루어 보아 난황막 단백질은 난자형성단계에 따라 변화한다는 사실을 알 수 있다.

• Clinical and Experimental Pediatrics
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• v.62 no.8
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• pp.292-296
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• 2019

Epidemiologic studies of Kawasaki disease (KD) have shown a new pattern or change of its occurrence suggestive of its pathophysiology or risk factors from the first patient with KD reported in 1961. The incidence of KD in Northeast Asian countries including Japan, South Korea, China, and Taiwan is 10-30 times higher than that in the United States and Europe. Knowing the true epidemiology of KD in each country and the availability of publications of KD epidemiology also could benefit general health care providers and general population. This would enable the early detection and treatment of KD, ultimately reducing the incidence of coronary artery complications and mortality. Therefore, efforts to investigate the true epidemiology of KD should be continued in every country using a questionnaire survey, National Health Insurance system data, or combined methods depending on each country's medical environment to ensure high-quality care of patients with KD.