Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Peptide Sequence Analysis of the CNBr-Digested 34-36 kd Sperminogen

  • Yu, Hyunkyung (Department of Biological Science and the Institute for Basic Science, Sungkyunkwan University) ;
  • Yi, Lee-S.-H. (Department of Biological Science and the Institute for Basic Science, Sungkyunkwan University)
  • Published : 2001.09.01
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Abstract

Sperminogen was purified from the acid extracts of boar spermatozoa and partial peptide sequence of the 34-36 kd sperminogen was determined. Acid extracts of boar spermatozoa was gel-filtered through Sephadex G-75, and the 34-36 kd sperminogen was purified by preparative SDS-PAGE. The sperminogen bands were sliced out, and 34-36 kd sperminogen were eluted from the gel fragments and was subjected to peptide sequencing. Since the amino termini were blocked for Edman degradation method, internal amino acid sequences of the eluted 34-36 kd sperminogen were obtained from CNBr-digested peptides of sperminogen. Among several bands resolved on tricine SDS-PAGE, 14, 22 and 26 kd peptides were subjected to peptide sequencing. The ana1yzed amino acid sequences of the 26 and 22 kd peptides showed high homologies with that of the zona pellucida binding protein, Sp38, and the analyzed amino acid sequence of the 14 kd peptide showed neither sequence homology nor similarity with any known proteins.

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References

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