• Title/Summary/Keyword: KC 수

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Development of PCR Diagnosis System for Plant Quarantine Seed-borne Wheat Streak Mosaic Virus (식물검역 종자전염 Wheat Streak Mosaic Virus의 PCR 검사시스템 개발)

  • Lee, Siwon;Kang, Eun-Ha;Chu, Yeon-Mee;Shin, Yong-Gil;Ahn, Tae-Young
    • Korean Journal of Microbiology
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    • v.49 no.2
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    • pp.112-117
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    • 2013
  • Wheat streak mosaic virus (WSMV), a member of the genus Tritimovirus in Potyviridae, severely impacts wheat and corn seed worldwide, but has yet to be detected in Korea, and hence, every effort should be made to prevent its introduction. To prevent WSMV from entering the country, it is necessary to prepare a specific, sensitive, simple, and fast detection method for routine application to plant quarantine procedures. For this reason, a two-step diagnosis system consisting of RT-PCR and nested PCR is being used for WSMV detection. In addition, a novel positive control was developed for use with the system. WSMV has been detected in seed sweet corn from Japan and seed wheat from USA by a two-step diagnosis system, the details of which are described in this study. After sequence analysis, similarities of 80.6 and 100.0% with other isolates were determined by BLAST. They showed the same topology, which was classified as 4 genotypes by various phylogenetic trees, using a poly protein encoding sequence amplification. In this analysis, WSMV-JSweet-corn2868 (JX845574) is classified as clade B, while WSMV-Uwheat1944-1 (KC754959) and WSMV-Uwheat1944-2 (KC754960) belong to clade D.

The Differences of Serum Homocysteine Levels in Mild Cognitive Impairment and Dementia of Alzheimer's Type with or without Depressive Symptoms (경도인지장애, 알쯔하이머형 치매 환자에서 우울증상 유무에 따른 혈중 호모시스테인의 차이)

  • Hwangbo, Ram;Kim, Hyun;Lee, Kang Joon
    • Korean Journal of Psychosomatic Medicine
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    • v.22 no.1
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    • pp.40-45
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    • 2014
  • Objectives : Mild cognitive impairment(MCI) and dementia of Alzheimer's type(AD) are characterized by progressive decline of cognitive abilities and a wide range of neuropsychiatric symptoms like depression. Among various diagnostic tools of AD, many studies showed that elevated levels of serum total homocysteine are associated with increased risk of developing Alzheimer's disease, depression and other neuropsychiatric disorders. We investigated whether elevated homocysteine concentrations are associated with depressive symptoms in MCI and AD. Methods : A total of 86 patients diagnosed with MCI or AD participated. Total serum homocysteine levels in fasting blood samples were measured. We examined cognitive symptoms by MMSE-KC, Global Deterioration Scale(GDS), Clinical dementia rating(CDR) and depressive symptoms by Korean version of Geriatric Depression Scale(K-GDS). Results : The total serum homocysteine levels were significantly higher in MCI with depression than in MCI without depression. There was no significant difference in the mean homocysteine levels between AD patients with depression and AD patients without depression. The total homocysteine levels showed a negative correlation with MMSE-KC and a positive correlation with CDR, GDS. Conclusions : These findings suggest that elevated homocysteine level is a risk factor for the decline of cognitive function and depression. We found a significant relationship between elevated serum homocysteine level and depressive symptoms in MCI. But our study had several limitations, thus more research is needed to confirm this finding.

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Studies on the Production of Protease by Aspergillus oryzae KC-15 and Characteristics of the Enzymes (Aspergillus oryzae KC-15에 의한 protease의 생산 및 그 효소의 특징에 관한 연구)

  • 이미자;정만재
    • Microbiology and Biotechnology Letters
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    • v.8 no.2
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    • pp.77-85
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    • 1980
  • This experiment was conducted to investigate the conditions for production and the characteristics of pretenses. Aspergillus oryzae KC-15, which is selected as a superior strain for the production of the protease, was used in this study. The results obtained were as follows: 1. The optimum culture time for the production of acid, neutral and alkaline protease on wheat bran medium were about 48, 48 and 72hr, respectively. The protease-produced by the strain were mainly alkaline and neutral one, but the production of acid protease was feeble extremely. 2. The addition of NaH$_2$PO$_4$, Na$_2$HPO$_4$, glucose, rice powder and Na-glutamate respectively to wheat bran media were effective for the production of alkaline and neutral protease, and the addition of (NH$_4$)$_2$HPO$_4$, glucose and rice powder respectively were effective for the production of acid protease. 3. Characteristics of professes(equation omitted) 4. As a heat resistance agent, NaH$_2$PO$_4$was the most effective one. The optimum amount of NaH$_2$PO$_4$was 10mg for alkaline and neutral protease, and 5mg for acid protease. 5. The heat resistance of the Protease by NaH$_2$PO$_4$was not recognized mostly above 6$0^{\circ}C$. 6. After the treatment of enzyme solution with 10mg of NaH$_2$PO$_4$for 30 minutes at 55$^{\circ}C$, the residual activities measured for alkaline, neutral and acid protease were 58, 57 and 55% respectively.

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Phylogenetic Analysis of Korean Black Cattle Based on the Mitochondrial Cytochrome b Gene (mtDNA cytochrome b에 기초한 한국흑우의 계통유전학적 분석)

  • Kim, Jae-Hwan;Byun, Mi Jung;Kim, Myung-Jick;Suh, Sang Won;Kim, Young-Sin;Ko, Yeoung-Gyu;Kim, Sung Woo;Jung, Kyoung-Sub;Kim, Dong-Hun;Choi, Seong-Bok
    • Journal of Life Science
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    • v.23 no.1
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    • pp.24-30
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    • 2013
  • The purpose of this study was to identify genetic polymorphisms of the mitochondrial cytochrome b (mtDNA cyt b) gene in Korean black (KB) cattle breed and to analyze the genetic relationship between the KB and other breeds. We determined the complete sequence of the mtDNA cyt b gene in 38 KB cattle. We also analyzed their genetic diversity, and phylogenetic analysis was performed by comparison with Korean cattle (KC, called Hanwoo) and breeds from China and Japan. A nucleotide substitution was detected in the KB cattle, and two haplotypes were defined. In the neighbor-joining (NJ) tree, the haplotypes of KB were located in Bos taurus lineage with those of KC, Japanese black (JB), Yanbian and Zaosheng breeds. However, the haplotypes of Chinese breeds, excluding Yanbian and Zaosheng, were separated into B. taurus and B. indicus lineages. In the NJ tree of breeds based on Dxy genetic distances, Chinese breeds mixed with B. taurus and B. indicus lineages were located between B. indicus and B. taurus lineages. KB was contained within B. taurus lineage and was determined to be genetically more closely related to two Chinese (Yanbian and Zaosheng) breeds than to KC and JB. The haplotype distribution and the results of the phylogenetic analysis suggest that KB and KC have genetic differences in their mtDNA cyt b gene sequences.

Implementation of Channel Coding System using Viterbi Decoder of Pipeline-based Multi-Window (파이프라인 기반 다중윈도방식의 비터비 디코더를 이용한 채널 코딩 시스템의 구현)

  • Seo Young-Ho;Kim Dong-Wook
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.9 no.3
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    • pp.587-594
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    • 2005
  • In the paper, after we propose a viterbi decoder which has multiple buffering and parallel processing decoding scheme through expanding time-divided imput signal, and map a FPGA, we implement a channel coding system together with PC-based software. Continuous input signal is buffered as order of decoding length and is parallel decoded using a high speed cell for viterbi decoding. Output data rate increases linearly with the cell formed the viterbi decoder, and flexible operation can be satisfied by programming controller and modifying input buffer. The tell for viterbi decoder consists of HD block for calculating hamming distance, CM block for calculating value in each state, TB block for trace-back operation, and LIFO. The implemented cell of viterbi decoder used 351 LAB(Logic Arrary Block) and stably operated in maximum 139MHz in APEX20KC EP20K600CB652-7 FPGA of ALTERA. The whole viterbi decoder including viterbi decoding cells, input/output buffers, and a controller occupied the hardware resource of $23\%$ and has the output data rate of 1Gbps.

Stress Path Dependent Deformation Characteristics of A Normally Consolidated Saturated Cohesive Soil (정규압밀 포화점성토의 응력경로에 따른 변형특성)

  • 권오엽;정인준
    • Geotechnical Engineering
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    • v.5 no.2
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    • pp.45-56
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    • 1989
  • The influence of stress path on the deformation characteristics of clay has been studied through a series of stress-path controlled triaxial tests on artificially sedimented and normally con- solidated Kaolinite. It has been found that there exists a critical stress increment ratio, Kc, in which stress·strain characteristics possesses a linear relationships and beyond Kc, strain hardening. A modified hyperbolic constitutive model for the strain hardening behavior has been formulated based on the Drnevich's hyperbolic function. And, a method of settlement analyses has been Proposed wherein the effect of stress path during consolidation is taken into account.

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A Study on Development of Seam Tracker with Weaving Function (위빙기능을 가진 용접선 추적장치의 개발에 관한 연구)

  • Kim, Hyun-Soo
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.13 no.4
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    • pp.113-117
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    • 2007
  • The study was performed on the development of system in which the bead width can be controlled. In order to control the bead width, we designed the automatic seam tracking device by attaching the probe type strain gauge sensor, motor driving slide and encoder to check the moving distance, and interface card connected MCU(80Cl96KC) upside the speed controllable carriage. Seam tracking experiments were done by changing the bead width. We compared and analyzed the sampling data which were obtained by output voltage of strain gauge sensor and rotary encoder pulse every 50ms.

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Speed Control of SRM by Pl Controller with Fuzzy Logic Modifier (Fuzzy Logic Modifier를 가진 Pl 제어기에 의한 스위치드 리럭턴스 전동기의 속도제어)

  • Kim, Bo-Hyung;Kim, Jae-Mun;Won, Chung-Yuen
    • Journal of IKEEE
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    • v.2 no.2 s.3
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    • pp.299-308
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    • 1998
  • In this paper, reliable switched reluctance motor(SRM) drive system and 4-rule based fuzzy logic modifier(FLM) of the conventional PI controller are presented. The i80C196KC, low-cost one-chip microcontroller is used for designing SRM drive controller which include the speed controller and the starting sequence. The fuzzy logic modifier which exhibits a stabilizing effects on the closed-loop system, has good robustness regarding the improperly tuned PI controller. The simulation and experimental results are performed to verify the capability of proposed control method on 6/4 salient type SRM.

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A Development of Sun Tracking Control System for Parabolic Trough Concentrator (PTC용 태양 추적 장치의 개발)

  • Park, Y.C.;Kwak, H.Y.;Kang, Y.H.
    • Solar Energy
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    • v.18 no.3
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    • pp.25-30
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    • 1998
  • A sun tracking controller for PTC(parabolic trough concentrator) is a control system used to orient the concentrator toward the sun always, so that the maximum energy can be collected. The work presented here is a design and development of microprocessor based sun tracking control system for PTC. Sun tracking control system consists of a sun sensor and a single axis tracking control system. 80c196KC based control system consists of an analog input unit, 24V DC servomotor drive unit, I/O unit. Sun sensor has been constructed using photodiode and can detect the sun located within ${\pm}50^{\circ}$ measured from the sun sensor normal direction. The sun tracking system developed is being implemented and shows a good sun tracking performance.

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A Novel Optimized PWM Method Based on the Selection of Pulse Position (펄스 위치 가변에 의한 취적 PWM 방식)

  • 최익;권순학;송중호;박귀태;황재호
    • The Transactions of the Korean Institute of Power Electronics
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    • v.3 no.1
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    • pp.8-14
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    • 1998
  • This paper describes a novel real-time based optimal Pulse Width Modulation (PWM) method suitable for microprocessor-based PWM inverters. Optimal switching patterns minimizing the performance index corresponding to the distortion between the reference and the controlled output voltages are decided by on-line calculation using the microprocessor-implemented control system. To show the effectiveness of the proposed PWM scheme, digital simulation studies and experiments using a 16-bit single-chip microcontroller (Intel 80C196KC) are performed. The results obtained from these simulation studies and experiments show that the proposed PWM scheme has better performance than the other methods such as the natural PWM and the direct PWM.