• 생명과학회지
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• 제23권8호
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• pp.978-983
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• 2013

Kinesin-II는 다양한 운반체들을 미세소관을 따라 운반하는 motor 단백질의 하나이다. Kinesin-II는 두 개의 motor 단백질 KIF3A와 KIF3B, 그리고 motor 단백질의 말단에 결합하는 kinesin superfamily-associated protein 3 (KAP3)로 구성되어 있다. KAP3는 Kinesin-II의 기능에 중요한 역할을 하는 것으로 알려져 있으나 명확한 기능은 아직 밝혀지지 않았다. 본 연구에서 KAP3와 결합하는 단백질을 분리하기 위하여 효모 two-hybrid system을 사용하여 탐색한 결과 HS-1-associated protein X-1 (HAX-1)을 분리하였다. KAP3은 HAX-1의 C-말단 부위와 결합하며, HAX-1은 KAP3의 C-말단부위와 결합함을 효모 two-hybrid assay로 확인하였다. 그러나, HAX-1는 KIF3A, KIF3B, KIF5B, 그리고 kinesin light chain (KLC)과는 결합하지 않았다. KAP3와 HAX-1의 단백질 결합은 glutathione S-transferase (GST) pull-down assay와 공동면역침강으로 추가 확인하였다. 생쥐의 뇌 파쇄액을 HAX-1 항체와 KIF3A 항체로 면역침강을 행한 결과 Kinesin-II의 구성단백질인 KIF3B와 KAP3가 같이 침강하였다. 이러한 결과들은 KAP3가 Kinesin-II와 HAX-1의 결합을 매개한다는 것을 시사한다.

• 한국발생생물학회지:발생과생식
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• 제4권1호
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• pp.95-100
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• 2000

환경에 방출되어 있는 많은 내분비교란물질들은 사람과 동물의 내분비계에 교란을 일으킬 수 있는 잠재력을 가진다. 뇌의 성분화는 생식소 호르몬 영향하에 비가역적으로 진행되며 흰쥐의 경우 이 시기는 임신말기에서 생후 7∼10일 가량이다. 최근에 본 연구진은 횐쥐의 뇌 성 분화의 결정적인 시기에 발현되는 KAP3유전자를 클로닝하였다 (Choi ＆ Lee, 1999). KAP3의 기능은 신경세포를 포함한 세포에서 aronal tansport를 조절하는 것으로 알려져 있다. 본 연구에서는 흰쥐 뇌 발생의 결정적인 시기에 내분비 교란물질인 Dichlorodiphenyl trichloroethane (DDT)가 KAP3유전자 발현과 성분화에 미치는 영향을 검토하였다. DDT에 노출된 임신 17일된 흰쥐 태아 암컷과 수컷의 뇌에서 KAP3 mRNA의 발현이 증가하였다. 그러나 출생후 DDT에 노출된 흰쥐 암컷과 수컷의 뇌에서는 KAP3 mRNA의 발현은 감소하였다. 또한 태어난 직후 DDT에 노출된 경우 체중이 현저히 감소하였으며 수정율도 DDT에 노출되지 않은 흰쥐에 비하여 크게 낮았다. 이러한 결과는 내분비 교란물질인 DDT가 뇌의 성 분화와 관련된 유전자인 KAP3의 전사에 영향을 미치며, 내분비 교란물질에 노출된 태아의 뇌 분화에서 독성을 보이는 것을 의미한다. 그리고 KAP3유전자는 동물의 신경세포의 발생에 미치는 내분비 교란 물질의 독성을 분자생물학적으로 연구하기 위한 유전자 지표로도 사용 가능하다고 생각된다.

• Animal cells and systems
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• 제5권4호
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• pp.327-331
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• 2001

There is a critical developmental period during which brain sexual differentiation proceeds irreversibly under the influence of gonadal hormone. Recently, kinesin superfamily-associated protein 3 (KAP3) gene expressed during the 'critical period' of rat brain differentiation was identified by us (Choi and Lee, 1999). KAP3 functions as a microtubule-based motor that transports membranous organelles anterogradely in cells, including neurons (Yamazaki et al., 1996). mRNA level of KAP3 gene markedly increased before the initiation of puberty. Neonatal treatment of estrogen clearly inhibited the prepubertal increase in KAP3 mRNA level (Choi and Lee, 1999). In the present study, we aimed to investigate the effects of polychlorinated biphenyls (PCBs), as endocrine disruptors (EDs) on the expression of KAP3 gene during the 'critical period' of rat brain development. In our data, PCBs significantly decreased the expression of KAP3 gene in the fetal (day 17) and the neonatal (day 6 after birth in) male and female rat brains. The body weight and the breeding ability were significantly decreased in the PCBs-exposed rats compared with the control. These results showed that PCBs affect the transcriptional level of brain sexual differentiation related gene, KAP3, in the fetal and the neonatal rat brains. The maternal exposure to the PCBs may lead to toxic response in embryonic brain sexual differentiation and breeding ability after sexual maturation. This study indicates that KAP3 gene may be useful as a gene marker to analyze the molecular mechanism of toxic response in the animal brain development and sexual maturation exposed to PCBs.

• 천문학회보
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• 제41권1호
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• pp.75.1-75.1
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• 2016

KAP82 (KHU Auto-guiding software Package for McDonald 82 inch telescope)는 천체망원경의 정밀한 추적을 가능하게 하는 가이드 프로그램으로, 미국 텍사스주에 위치한 McDonald 천문대의 82 inch 망원경에 장착된 광학 및 적외선 영역의 관측기기 SQUEAN (SED camera for Quasars in EArly uNiverse)과 함께 개발되었다. KAP82는 지난 한 해 동안 두 차례의 개정을 통해 프로그램 작동의 안정성을 확보하고, 동시에 가이드의 성능에도 많은 개선이 이루어졌다. 대상 별 중심을 찾는 알고리즘에 따라 KAP82 1.0에서는 가중 평균(weighted mean)을, KAP82 2.0에서는 산술평균을 활용해 자체 개발한 J-J 함수를 사용해 가이드를 구현한 것이 특징이다. 이번에 개발한 KAP82 3.0은 가이드 알고리즘으로 가우스 함수를 채택하고, 제조사가 다른 다수의 상용 CCD카메라 및 망원경과 연결이 가능한 ASCOM Platform에서 작동하므로, 다른 시스템에도 쉽게 적용할 수 있는 장점이 있다. 본 포스터에서는 KAP82 3.0을 소개하면서 기존 KAP82버전들과 KAP82 3.0의 차이로 알아본 서로 다른 알고리즘에 따른 가이드의 정확성을 비교 분석한 결과를 제시한다.

• 한국융합학회논문지
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• 제10권6호
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• pp.73-78
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• 2019

본 연구는 국내 의료기관 500명의 식품 취급자들의 식품안전에 관한 지식, 인식 및 행태 수준을 평가하고 지식, 인식, 행태와 사회인구학적 특성과의 연관성을 분석하였다. 자가보고 설문지는 식품안전에 관한 지식 15문항, 인식 15문항, 행태 20문항 등 총 50문항으로 구성되었으며 연령별, 교육수준별, 경력별에 따른 지식, 인식, 행태와 KAP과의 관련성분석은 t 또는 ANOVA test를 실시하였다. 분석 결과 식품안전에 관한 평균 지식점수는 3.25, 인식 3.65, 행태 3.36으로 나타났다. 연령그룹별 식품안전에 관한 지식, 인식, KAP score는 차이가 나타났으며, KAP과 교육수준과의 관련성은 교육수준이 증가할수록 KAP score가 증가하는 것을 확인하였다(P < 0.05). 식품위해를 최소화하기 위해서 식품 취급자에게 공중보건학적차원의 지속적인 식품안전 예방교육 및 프로그램을 제공해야 할 것으로 사료된다.

• Animal cells and systems
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• 제3권3호
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• pp.319-329
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• 1999

Onset of female puberty follows a series of prepubertal cellular and molecular events including changes of synaptic plasticity, synthetic and releasing activity and gene expression. Dramatic increase of gonadal steroid level is one of the most prominent changes before the onset of puberty. Based on the importance of steroid feedback upon the hypothalamus, we adopted an estrogen sterilized rat (ESR) model where 100 ng of 17$\eta$-estradiol were administered into neonatal pubs for 7 days after birth. To identify genes involved in the onset of female puberty, we applied PCR differential display using RNA samples derived from ESR and control rat hypothalami. About 100 out of more than 1000 RNA species examined displayed differential expression patterns between a 60-day old control rat and ESR. Sequence analysis of differentially amplified PCR products showed homology with genes such as mouse kinesin superfamily-associated protein 3 (KAP3) and several cDNAs previously described by others in mouse and human tissues. Several gene products such as 2-1 and 8-1 corresponded to novel DNA sequences. We analyzed mRNA levels of KAP3, 2-1 and 8-1 genes in the hypothalami derived from neonatal, 6-, 28-, 31-, and 40-day old rats. Northern blot analysis showed that mRNAs of KAP3, 2-1 and 8-1 genes were markedly increased before the initiation of puberty. Neonatal treatment of estrogen clearly inhibited prepubertal increases in KAP3, 2-1 and 8-1 mRNA levels. Therefore, these genes may play important roles in the initiation of hypothalamic puberty. In addition, intracerebroventricular (icv) injection of antisense KAP3 oligodeoxynucleotide (ODN) clearly delayed puberty initiation determined by vaginal opening, which further confirmed that KAP3 plays an important role in the regulation of puberty initiation.

• 한국환경보건학회지
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• 제22권1호
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• pp.65-81
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• 1996

This study was conducted from march 20 through April 20, 1992, jn order to figure out the factors affecting behavior of the people on environmental health. 1, 261 people were selected by sampling from Seoul, Pusan, Taegu, Taejon and Kwangju. Questionnaire forms were prepared and the persons selected were-interviewed by trained interviewers. The data collected were analyzed in order to determine factors affecting kowledge, attitude and practice on environmental health in major cities in Korea. The major results are as follows: 1. The people who got high KAP score were found not to practice what they know. The people who got lower scores were found to use their knowledge in practical life. 2. Correlation between knowledge, attitude, and practice (KAP) on environmental health was statistically significant (p < 0.001). 3. The levels of KAP on environmental health affected by variables of gender and marital status were statistically significant (p < 0.01). 4. Variables of education, occupation, religion and economic status affected the levels of KAP significantly (p < 0.01). 5. The levels of KAP on environmental health by variables of health education on environmental health were statistically significant (p < 0.001). 6. The levels of KAP of the people on environmental health by demographic variables showed a reverse relation statistically (p < 0.001). 7. The levels of KAP of the people on environmental health had correlation with education level and the KAP level had correlation with knowledge (p < 0.001). 8. The KAP levels of the people on environmental health had correlation with environmental health education (p < 0.001). 9. The total variables affecting KAP of the people on environmental health had 14% variance and environmental health education was the highest($\beta$=0.23827), education level was the next($\beta$=0.12442), and economic status was ($\beta$=-0.06970), age ($\beta$=-0.06710) and print media ($\beta$=0.06539). 10. The variables most affecting KAP of the people on environmental health were environmental health education(r=0.2980) and education(r=0.2419) and the next were age, marital status, religion, electronic media, print media, place of birth and gender. 11. The most important variable affecting KAP level of the people on environmental health was education level.

• 보건교육건강증진학회지
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• 제6권2호
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• pp.4-22
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• 1989

This study aims to explore the relationship between housewives' health KAP level and the physical health of families. The data used in this study are obtained from the Last Evaluation Program of the National Note for Health surveyed in July, 1989. The respondents for this study are 770 housewives residing in Chunan and Buan. The reason why this study focus on KAP level of housewives is to find out whether a housewife as a emotional supporter contributes to the physical health of her families. A housewife gives her families emotional satisfaction insteade of economic satisfaction. So she has the most interest in family health among the members of her family. Therefore, housewife's KAP level will influence her family health. The independent variables chosen for the analysis are the general characteristic variables and KAP level. And the dependent variable is the physical health of families which excluded psychosocial one. This level of family health includes weight for family health and seriouseness of disease. The result of this study was summarized as follows. (1) KAP level was significantly correlated with variables which have mainly the socioeconomic characteristics. The variables were: area of residence, education level occupation, self assessment on wealth, and exposure to mass communication. (2) In the analysis of relationship between the general characteristic variables and family health, family health was significantly correlated with almost all variables. The variables were: Presence of the aged, Health status, Experience in disease, Self assessment on health, No. of families, Occupation, Education level, Self assessment on wealth, Concern on health, and Exposure to mass communication. (3) In the analysis of relationship between family health and KAP level, family health was not significantly correlated with KAP level but. (4) Also in the stepwise regression analysis, the general variables account for about 32.1 percent of the variance in the dependent variable, family health. The variable with the greatest explanatory power was presence of the aged. On the contrary, KAP level explain about 0.4 percent of the variance in the dependent variable. In sum, the study shows that housewives' health KAP level has relatively weak relationship with the physical health of families

• 한국콘텐츠학회논문지
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• 제11권3호
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• pp.302-308
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• 2011

혈관 조영 검사 및 중재적 방사선 시술을 통한 환자에게 주어지는 피폭선량에 대해 대뇌를 중심으로 환자 체형을 표준화하여 검사 및 시술 차이에 따른 투시시간, 면적 선량률(kerma area product rate)을 관측하고 참고 기준치를 비교하고자 하였다. 그 결과 질병 분류에 따른 면적 선량률은 지주막하출혈(subarachnoid hemorrhage)이 가장 높았고, 뇌동맥류가 가장 낮았다. 또한 검사 및 시술에 따른 면적 선량률은 양쪽 내경동맥, 양쪽 총경동맥 및 추골동맥으로 하는 방법이 가장 높았으며, guglielmi detachable coil(GDC)이 가장 낮게 나타났다. 따라서 이를 혈관 조영 검사 및 중재적 방사선 시술시 환자 피폭 선량에 대한 참고 기준치로 이용할 수 있을 것이며 향후 환자 피폭 선량 교육과 관리에 유용할 것이라 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권3호
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• pp.328-337
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• 2017

Objective: An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness. Methods: In previous work, we constructed a skin cDNA library and isolated a full-length cDNA clone termed KAP11.1. On this basis, we conducted a series of bioinformatics analysis. Tissue distribution of KAP11.1 mRNA was performed using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The expression of KAP11.1 mRNA in primary and secondary hair follicles was performed using real-time PCR (real-time polymerase chain reaction) analysis. The expression location of KAP11.1 mRNA in primary and secondary hair follicles was performed using in situ hybridization. Results: Bioinformatics analysis showed that KAP11.1 gene encodes a putative 158 amino acid protein that exhibited a high content of cysteine, serine, threonine, and valine and has a pubertal mammary gland) structural domain. Secondary structure prediction revealed a high proportion of random coils (76.73%). Semi-quantitative RT-PCR showed that KAP11.1 gene was expressed in heart, skin, and liver, but not expressed in spleen, lung and kidney. Real time PCR results showed that the expression of KAP11.1 has a higher expression in catagen than in anagen in the primary hair follicles. However, in the secondary hair follicles, KAP11.1 has a significantly higher expression in anagen than in catagen. Moreover, KAP11.1 gene has a strong expression in inner root sheath, hair matrix, and a lower expression in hair bulb. Conclusion: We conclude that KAP11.1 gene may play an important role in regulating the fiber diameter.