Browse > Article
http://dx.doi.org/10.5713/ajas.16.0078

Molecular characterization and expression pattern of a novel Keratin-associated protein 11.1 gene in the Liaoning cashmere goat (Capra hircus)  

Jin, Mei (Faculty of Life Science, Liaoning Normal University, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery)
Cao, Qian (Faculty of Life Science, Liaoning Normal University, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery)
Wang, Ruilong (Faculty of Life Science, Liaoning Normal University, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery)
Piao, Jun (Faculty of Life Science, Liaoning Normal University, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery)
Zhao, Fengqin (Faculty of Life Science, Liaoning Normal University, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery)
Piao, Jing'ai (Faculty of Life Science, Liaoning Normal University, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.30, no.3, 2017 , pp. 328-337 More about this Journal
Abstract
Objective: An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness. Methods: In previous work, we constructed a skin cDNA library and isolated a full-length cDNA clone termed KAP11.1. On this basis, we conducted a series of bioinformatics analysis. Tissue distribution of KAP11.1 mRNA was performed using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The expression of KAP11.1 mRNA in primary and secondary hair follicles was performed using real-time PCR (real-time polymerase chain reaction) analysis. The expression location of KAP11.1 mRNA in primary and secondary hair follicles was performed using in situ hybridization. Results: Bioinformatics analysis showed that KAP11.1 gene encodes a putative 158 amino acid protein that exhibited a high content of cysteine, serine, threonine, and valine and has a pubertal mammary gland) structural domain. Secondary structure prediction revealed a high proportion of random coils (76.73%). Semi-quantitative RT-PCR showed that KAP11.1 gene was expressed in heart, skin, and liver, but not expressed in spleen, lung and kidney. Real time PCR results showed that the expression of KAP11.1 has a higher expression in catagen than in anagen in the primary hair follicles. However, in the secondary hair follicles, KAP11.1 has a significantly higher expression in anagen than in catagen. Moreover, KAP11.1 gene has a strong expression in inner root sheath, hair matrix, and a lower expression in hair bulb. Conclusion: We conclude that KAP11.1 gene may play an important role in regulating the fiber diameter.
Keywords
Liaoning Cashmere Goat; Keratin-associated Protein 11.1 (KAP11.1); Expression Pattern; Tissue-specific; Gene; Hair;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Li CQ, Yin J, Zhang YJ, et al. Comparative study on skin and hair follicles cycling between inner mongolia and Liaoning cashmere goats. Acta Vet Zootec Sin 2005;36:674-9.
2 Jin M, Bai X, Zhang W, Gao WB, Wang W. Study on sequence variation of mitochondrial cytochrome b gene and phlogenetic relationships of two kinds of cashmere goats. Sci Agric Sin 2006;39:1897-901.
3 Zhao M, Chen H, Wang X, et al. PCR-SSCP and DNA sequencing detecting two silent SNPs at KAP8.1 gene in the cashmere goat. Mol Biol Rep 2009;36:1387-91.   DOI
4 Zhou H, Gong H, Yan W, Luo Y, Hickford JG. Identification and sequence analysis of the keratin-associated protein 24-1 (KAP24-1) gene homologue in sheep. Gene 2012;511:62-5.   DOI
5 Wu DD, Irwin DM, Zhang YP. Molecular evolution of the keratin associated protein gene family in mammals, role in the evolution of mammalian hair. BMC Evol Biol 2008;8:1471-2148.
6 Gong H, Zhou H, Dyer JM, Hickford JG. Identification of the ovine KAP11-1 gene (KRTAP11-1) and genetic variation in its coding sequence. Mol Biol Rep 2011;38:5429-33.   DOI
7 Gong H, Zhou H, Dyer JM, Plowman JE, Hickford JG. Identification of the keratin-associated protein 13-3 (KAP13-3) gene in sheep. Open J Genet 2011;1:60-4.   DOI
8 Jin M, Hu JH. The breeding situation and development direction of Liaoning cashmere goat. Chinese J Anim Sci 2005;41:54-6.
9 Fratini A, Powell BC, Hynd PI, Keough RA, Rogers GE. Dietary cysteine regulates the levels of mRNAs encoding a family of cysteine-rich proteins of wool. J Invest Dermatol 1994;102:178-85.   DOI
10 Liu GF, Tian KC, Zhang EP, Huang XX, Zhang YH. Candidate gene analysis of high quality merino sheep. Hereditas 2007;29: 70-4.
11 Hiroki F, Atsushi F, Muhammad F, Masaaki I, Yutaka S. Characterization of the human hair keratin-associated protein 2 (KRTAP2) gene family. J Investe Dermatol 2012;132:1806-13.   DOI
12 Shah RM, Ganai TA, Sheikh FD, et al. Characterization and polymorphism of Keratin associated protein 1.4 gene in goats. Gene 2013;518:431-42.   DOI
13 Shibuya K, Obayashi I, Asakawa S, et al. A cluster of 21 keratinassociated protein genes within introns of another gene on human chromosome 21q22.3. Genomics 2004;83:679-93.   DOI
14 Michael AR, Lutz L, Hermelita W, et al. Characterization of a first domain of human high glycine-tyrosine and high sulfer keratinassociated protein (KAP) genes on chromosome 21q22.1. Biol Chem 2002;277:48993-9002.   DOI
15 Powell BC, Nesci A, Rogers GE. Regulation of keratin gene expression in hair follicle differentiation. Ann NY Acad Sci 1991;642:1-20.
16 Refaut NW, Pearson AJ, Nixon AJ, Wheeler TT, Wilkins RJ. Identification of diffemtially expressed genes during a wool follicle growth cycle induced by prolactin. J Invest Dernmtol 1999;113:865-72.   DOI
17 Hashimoto O, Yonezawa T, Sugiyama Y, Kawaminami M, Hasegawa Y. Molecular clonin and expresion of Canine Prolactin gene. Exp Anim 2010;59:643-6.   DOI
18 Rogers MA, Winter H, Langbein L, et al. Characterization of human KAP24.1, a cuticular hair keratin-associated protein with unusual amino-acid composition and repeat structure. J Invest Dermatol 2007;127:1197-204.   DOI
19 Rogers MA, Langbein L, Praetzel-Wunder S, Giehl K. Characterization and expression analysis of the hair keratin associated protein KAP26.1. Br J Dermatol 2008;159:725-9.   DOI
20 Langbein L, Rogers MA, Winter H, Praetzel S, Schweizer J. The catalog of human hair keratins. II. Expression of the six type II members in the hair follicle and the combined catalog of human type I and II keratins. J Biol Chem 2001;276:35123-32.   DOI
21 Powell BC, Arthur J, Nesci A. Characterization of a gene encoding a cysteine-rich keratin associated protein synthesized late in rabbit hair follicle differentiation. Differentiation 1995;58:227-32.   DOI
22 Powell BC, Rogers GE. The role of keratin proteins and their genes in the growth, structure and properties of hair. EXS 1997;78:59-148.
23 Gong H, Zhou H, Mckenzie GW, et al. Emerging issues with the current keratin-associated protein nomenclature. Int J Trichol 2010; 2:104-5.   DOI
24 Rogers MA, Langbein L, Praetzel-Wunder S, Winter H, Schweizer J. Human hair keratin associated proteins (KAPs). Int Rev Cytol 2006;251:209-63.
25 Kuhn FC, Lassing A, Range M, et al. Pmg-1 and pmg-2 constitute a novel family of KAP genes differentially expressed during skin and mammary gland development. Mech Dev 1999;86:193-6.   DOI
26 Toivola DM, Ku NO, Resurreccion EZ, Wright TL, Omary MB. Keratin 8 and 18 hyperphosphorylation is a maker of progression of human liver disease. Hepatology 2004;40:459-66.   DOI