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http://dx.doi.org/10.5352/JLS.2013.23.8.978

Kinesin Superfamily-associated Protein 3 (KAP3) Mediates the Interaction between Kinesin-II Motor Subunits and HS-1-associated Protein X-1 (HAX-1) through Direct Binding  

Jang, Won Hee (Departments of Biochemistry, College of Medicine and UHRC, Inje University)
Seog, Dae-Hyun (Departments of Biochemistry, College of Medicine and UHRC, Inje University)
Publication Information
Journal of Life Science / v.23, no.8, 2013 , pp. 978-983 More about this Journal
Abstract
Kinesin-II, a molecular motor, consists of two different motor subunits, KIF3A and KIF3B, and one large kinesin superfamily-associated protein 3 (KAP3), forming a heterotrimeric complex. KAP3 is associated with the tail domains of motor subunits. However, its exact role remains unclear. Here, we demonstrated KAP3 binding to the carboxyl (C)-terminal tail region of HS-associated protein X-1 (HAX-1). HAX-1 bound to the C-terminal region of KAP3, but not to KIFs (KIF3A, KIF3B, and KIF5B) and the kinesin light chain (KLC) in the yeast two-hybrid assays. The interaction was further confirmed in the glutathione S-transferase (GST) pull-down assay and by co-immunoprecipitation. Anti- HAX-1 antibody as well as anti-KIF3A antibody co-immunoprecipitated KIF3B and KAP3 from mouse brain extracts. These results suggest that KAP3 could mediate the interaction between Kinesin-II and HAX-1.
Keywords
Microtubule motors; kinesin-II; kinesin superfamily-associated protein 3 (KAP3); HS-associated protein X-1 (HAX-1); adaptor proteins; protein-protein interaction;
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1 Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K. 1998. Current Protocols in Molecular Biology. John Wiley & Sons.
2 Brady, S. T. 1985. A novel brain ATPase with properties expected for the fast axonal transport motor. Nature 317, 73-75.   DOI   ScienceOn
3 Chao, J. R., Parganas, E., Boyd, K., Hong, C. Y., Opferman, J. T. and Ihle, J. N. 2008. HAX1-mediated processing of HtrA2 by Parl allows survival of lymphocytes and neurons. Nature 452, 98-102.   DOI   ScienceOn
4 Davenport, J. R., Watts, A. J., Roper, V. C., Croyle, M. J., van Groen, T., Wyss, J. M., Nagy, T. R., Kesterson, R. A. and Yoder, B. K. 2007. Disruption of intraflagellar transport in adult mice leads to obesity and slow-onset cystic kidney disease. Curr Biol 17, 1586-1594.   DOI   ScienceOn
5 Fadeel, B. and Grzybowska, E. 2009. HAX-1: a multifunctional protein with emerging roles in human disease. Biochim Biophys Acta 1790, 1139-1148.   DOI   ScienceOn
6 Gallagher, A. R., Cedzich, A., Gretz, N., Somlo, S. and Witzgall, R. 2000. The polycystic kidney disease protein PKD2 interacts with HAX-1, a protein associated with the actin cytoskeleton. Proc Natl Acad Sci USA 97, 4017-4022.   DOI   ScienceOn
7 Hirokawa, N., Niwa, S. and Tanaka, Y. 2010. Molecular motors in neurons: transport mechanisms and roles in brain function, development, and disease. Neuron 68, 610-638.   DOI   ScienceOn
8 Hirokawa, N., Tanaka, Y. and Okada, Y. 2012. Cilia, KIF3 molecular motor and nodal flow. Curr Opin Cell Biol 24, 31-39.   DOI   ScienceOn
9 Hirokawa, N. 1998. Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science 279, 519-526.   DOI   ScienceOn
10 Kanai, Y., Okada, Y., Tanaka, Y., Harada, A., Terada, S. and Hirokawa, N. 2000. KIF5C, a novel neuronal kinesin enriched in motor neurons. J Neurosci 20, 6374-6384.
11 Kim, S. J., Lee, C. H., Park, H. Y., Yea, S. S., Jang, W. H., Lee, S. K., Park, Y. H., Cha, O. S., Moon, I. S. and Seog, D. H. 2007. JSAP1 interacts with kinesin light chain 1 through conserved binding segments. J Life Sci 17, 889-895.   과학기술학회마을   DOI   ScienceOn
12 Kozminski, K. G., Beech, P. L. and Rosenbaum, J. L. 1995. The Chlamydomonas kinesin-like protein FLA10 is involved in motility associated with the flagellar membrane. J Cell Biol 131, 1517-1527.   DOI
13 Lin, F., Hiesberger, T., Cordes, K., Sinclair, A. M., Goldstein, L. S., Somlo, S. and Igarashi, P. 2003. Kidney-specific inactivation of the KIF3A subunit of kinesin-II inhibits renal ciliogenesis and produces polycystic kidney disease. Proc Natl Acad Sci USA 100, 5286-5291.   DOI   ScienceOn
14 Modem, S. and Reddy, T. R. 2008. An anti-apoptotic protein, HAX-1, inhibits the HIV-1 rev function by altering its sub-cellular localization. J Cell Physiol 214, 14-19.   DOI   ScienceOn
15 Mueller, J., Perrone, C. A., Bower, R., Cole, D. G. and Porter, M. E. 2005. The FLA3 KAP subunit is required for localization of kinesin-2 to the site of flagellar assembly and processive anterograde intraflagellar transport. Mol Biol Cell 16, 1341-1354.   DOI   ScienceOn
16 Ou, G., Koga, M., Blacque, O. E., Murayama, T., Ohshima, Y., Schafer, J. C., Li, C., Yoder, B. K., Leroux, M. R. and Scholey, J. M. 2007. Sensory ciliogenesis in Caenorhabditis elegans: assignment of IFT components into distinct modules based on transport and phenotypic profiles. Mol Biol Cell 18, 1554-1569.   DOI   ScienceOn
17 Ramsay, A. G., Keppler, M. D., Jazayeri, M., Thomas, G. J., Parsons, M., Violette, S., Weinreb, P., Hart, I. R. and Marshall, J. F. 2007. HS1-associated protein X-1 regulates carcinoma cell migration and invasion via clathrin-mediated endocytosis of integrin alphavbeta6. Cancer Res 67, 5275-5284.   DOI   ScienceOn
18 Sarpal, R., Todi, S. V., Sivan-Loukianova, E., Shirolikar, S., Subramanian, N., Raff, E. C., Erickson, J. W., Ray, K. and Eberl, D. F. 2003. Drosophila KAP interacts with the kinesin II motor subunit KLP64D to assemble chordotonal sensory cilia, but not sperm tails. Curr Biol 13, 1687-1696.   DOI   ScienceOn
19 Shimizu, K., Kawabe, H., Minami, S., Honda, T., Takaishi, K., Shirataki, H. and Takai, Y. 1996. SMAP, an Smg GDS-associating protein having arm repeats and phosphorylated by Src tyrosine kinase. J Biol Chem 271, 27013-27017.   DOI   ScienceOn
20 Suzuki, Y., Demoliere, C., Kitamura, D., Takeshita, H., Deuschle, U. and Watanabe, T. 1997. HAX-1, a novel intracellular protein, localized on mitochondria, directly associates with HS1, a substrate of Src family tyrosine kinases. J Immunol 158, 2736-2744.
21 Tabish, M., Siddiqui, Z. K., Nishikawa, K. and Siddiqui, S. S. 1995. Exclusive expression of C. elegans osm-3 kinesin gene in chemosensory neurons open to the external environment. J Mol Biol 247, 377-389.   DOI   ScienceOn
22 Takeda, S., Yamazaki, H., Seog, D. H., Kanai, Y., Terada, S. and Hirokawa, N. 2000. Kinesin superfamily protein 3 (KIF3) motor transports fodrin-associating vesicles important for neurite building. J Cell Biol 148, 1255-1265.   DOI   ScienceOn
23 Yamazaki, H., Nakata, T., Okada, Y. and Hirokawa, N. 1996. Cloning and characterization of KAP3: a novel kinesin superfamily- associated protein of KIF3A/3B. Proc Natl Acad Sci USA 93, 8443-8448.   DOI   ScienceOn