• Journal of Life Science
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• v.23 no.8
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• pp.978-983
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• 2013

Kinesin-II, a molecular motor, consists of two different motor subunits, KIF3A and KIF3B, and one large kinesin superfamily-associated protein 3 (KAP3), forming a heterotrimeric complex. KAP3 is associated with the tail domains of motor subunits. However, its exact role remains unclear. Here, we demonstrated KAP3 binding to the carboxyl (C)-terminal tail region of HS-associated protein X-1 (HAX-1). HAX-1 bound to the C-terminal region of KAP3, but not to KIFs (KIF3A, KIF3B, and KIF5B) and the kinesin light chain (KLC) in the yeast two-hybrid assays. The interaction was further confirmed in the glutathione S-transferase (GST) pull-down assay and by co-immunoprecipitation. Anti- HAX-1 antibody as well as anti-KIF3A antibody co-immunoprecipitated KIF3B and KAP3 from mouse brain extracts. These results suggest that KAP3 could mediate the interaction between Kinesin-II and HAX-1.

• Development and Reproduction
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• v.4 no.1
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• pp.95-100
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• 2000

A large number of man-made chemicals that have been released into the environment have the potential to disrupt the endocrine system of animals and humans. There is a critical developmental period during which sexual brain differentiation proceeds irreversibly under the influence of gonadal hormone. Recently we identified KAP3 gene expressed during the critical period of rat brain sexual differentiation. KAP3 functions as a microtubule-based motor that transports membranous organelles anterogradely in cells, including neurons. In the present study, we aimed to investigate the effect of endocrine disrupter, Dichlorodiphenyl trichloroethane (DDT), on the KAP3 gene expression during critical period of rat brain development. Maternal exposure to DDT increased the level of KAP3 mRNA in male and female fetus brains when examined on the gestational day 17 (GDl7). In postnatal day 6, DDT suppressed the expression of KAP3 gene in male and female rat brain. Also, the body weight and fertilization rate were decreased in the DDT exposured rats. These results showed that endocrine disrupter, DDT, can affect the transcriptional level of brain sexual differentiation related gene, KAP3, in the prenatal and the neonatal rat brain and that maternal exposure to endocrine disruptors may lead to a toxic response in embryonic differentiation of brain. And so KAP3 gene may be used a gene maker to analyse the molecular mechanism for toxic response in animal nerve tissues exposed to endocrine disruptors.

• Animal cells and systems
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• v.5 no.4
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• pp.327-331
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• 2001

There is a critical developmental period during which brain sexual differentiation proceeds irreversibly under the influence of gonadal hormone. Recently, kinesin superfamily-associated protein 3 (KAP3) gene expressed during the 'critical period' of rat brain differentiation was identified by us (Choi and Lee, 1999). KAP3 functions as a microtubule-based motor that transports membranous organelles anterogradely in cells, including neurons (Yamazaki et al., 1996). mRNA level of KAP3 gene markedly increased before the initiation of puberty. Neonatal treatment of estrogen clearly inhibited the prepubertal increase in KAP3 mRNA level (Choi and Lee, 1999). In the present study, we aimed to investigate the effects of polychlorinated biphenyls (PCBs), as endocrine disruptors (EDs) on the expression of KAP3 gene during the 'critical period' of rat brain development. In our data, PCBs significantly decreased the expression of KAP3 gene in the fetal (day 17) and the neonatal (day 6 after birth in) male and female rat brains. The body weight and the breeding ability were significantly decreased in the PCBs-exposed rats compared with the control. These results showed that PCBs affect the transcriptional level of brain sexual differentiation related gene, KAP3, in the fetal and the neonatal rat brains. The maternal exposure to the PCBs may lead to toxic response in embryonic brain sexual differentiation and breeding ability after sexual maturation. This study indicates that KAP3 gene may be useful as a gene marker to analyze the molecular mechanism of toxic response in the animal brain development and sexual maturation exposed to PCBs.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.1
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• pp.75.1-75.1
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• 2016

KAP82 (KHU Auto-guiding software Package for McDonald 82 inch telescope)는 천체망원경의 정밀한 추적을 가능하게 하는 가이드 프로그램으로, 미국 텍사스주에 위치한 McDonald 천문대의 82 inch 망원경에 장착된 광학 및 적외선 영역의 관측기기 SQUEAN (SED camera for Quasars in EArly uNiverse)과 함께 개발되었다. KAP82는 지난 한 해 동안 두 차례의 개정을 통해 프로그램 작동의 안정성을 확보하고, 동시에 가이드의 성능에도 많은 개선이 이루어졌다. 대상 별 중심을 찾는 알고리즘에 따라 KAP82 1.0에서는 가중 평균(weighted mean)을, KAP82 2.0에서는 산술평균을 활용해 자체 개발한 J-J 함수를 사용해 가이드를 구현한 것이 특징이다. 이번에 개발한 KAP82 3.0은 가이드 알고리즘으로 가우스 함수를 채택하고, 제조사가 다른 다수의 상용 CCD카메라 및 망원경과 연결이 가능한 ASCOM Platform에서 작동하므로, 다른 시스템에도 쉽게 적용할 수 있는 장점이 있다. 본 포스터에서는 KAP82 3.0을 소개하면서 기존 KAP82버전들과 KAP82 3.0의 차이로 알아본 서로 다른 알고리즘에 따른 가이드의 정확성을 비교 분석한 결과를 제시한다.

• Journal of the Korea Convergence Society
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• v.10 no.6
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• pp.73-78
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• 2019

This study aimed to assess the level of food safety knowledge, attitude and practices (KAP) among 500 Food handlers of hospitals in Korea and to explore the association between their knowledge, attitude and practices (KAP) and the socio-demographic characteristics. Self-reported questionnaire contains 50 questions: 15 for knowledge, 15 for attitude, and 20 for practice on food safety. The results showed that the overall mean of the knowledge score is 3.25, attitude 3.65, and practice 3.36 respectively. There was a significant difference between the mean score for knowledge, practice and overall KAP in three aged groups. Also, the overall KAP scores were significantly (p < 0.05) affected by the education level, where the average scores increased with the level of education. Public health preventive education and program should be provided to the food handlers in order to minimize foodborne hazards.

• Animal cells and systems
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• v.3 no.3
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• pp.319-329
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• 1999

Onset of female puberty follows a series of prepubertal cellular and molecular events including changes of synaptic plasticity, synthetic and releasing activity and gene expression. Dramatic increase of gonadal steroid level is one of the most prominent changes before the onset of puberty. Based on the importance of steroid feedback upon the hypothalamus, we adopted an estrogen sterilized rat (ESR) model where 100 ng of 17$\eta$-estradiol were administered into neonatal pubs for 7 days after birth. To identify genes involved in the onset of female puberty, we applied PCR differential display using RNA samples derived from ESR and control rat hypothalami. About 100 out of more than 1000 RNA species examined displayed differential expression patterns between a 60-day old control rat and ESR. Sequence analysis of differentially amplified PCR products showed homology with genes such as mouse kinesin superfamily-associated protein 3 (KAP3) and several cDNAs previously described by others in mouse and human tissues. Several gene products such as 2-1 and 8-1 corresponded to novel DNA sequences. We analyzed mRNA levels of KAP3, 2-1 and 8-1 genes in the hypothalami derived from neonatal, 6-, 28-, 31-, and 40-day old rats. Northern blot analysis showed that mRNAs of KAP3, 2-1 and 8-1 genes were markedly increased before the initiation of puberty. Neonatal treatment of estrogen clearly inhibited prepubertal increases in KAP3, 2-1 and 8-1 mRNA levels. Therefore, these genes may play important roles in the initiation of hypothalamic puberty. In addition, intracerebroventricular (icv) injection of antisense KAP3 oligodeoxynucleotide (ODN) clearly delayed puberty initiation determined by vaginal opening, which further confirmed that KAP3 plays an important role in the regulation of puberty initiation.

• Journal of Environmental Health Sciences
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• v.22 no.1
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• pp.65-81
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• 1996

This study was conducted from march 20 through April 20, 1992, jn order to figure out the factors affecting behavior of the people on environmental health. 1, 261 people were selected by sampling from Seoul, Pusan, Taegu, Taejon and Kwangju. Questionnaire forms were prepared and the persons selected were-interviewed by trained interviewers. The data collected were analyzed in order to determine factors affecting kowledge, attitude and practice on environmental health in major cities in Korea. The major results are as follows: 1. The people who got high KAP score were found not to practice what they know. The people who got lower scores were found to use their knowledge in practical life. 2. Correlation between knowledge, attitude, and practice (KAP) on environmental health was statistically significant (p < 0.001). 3. The levels of KAP on environmental health affected by variables of gender and marital status were statistically significant (p < 0.01). 4. Variables of education, occupation, religion and economic status affected the levels of KAP significantly (p < 0.01). 5. The levels of KAP on environmental health by variables of health education on environmental health were statistically significant (p < 0.001). 6. The levels of KAP of the people on environmental health by demographic variables showed a reverse relation statistically (p < 0.001). 7. The levels of KAP of the people on environmental health had correlation with education level and the KAP level had correlation with knowledge (p < 0.001). 8. The KAP levels of the people on environmental health had correlation with environmental health education (p < 0.001). 9. The total variables affecting KAP of the people on environmental health had 14% variance and environmental health education was the highest($\beta$=0.23827), education level was the next($\beta$=0.12442), and economic status was ($\beta$=-0.06970), age ($\beta$=-0.06710) and print media ($\beta$=0.06539). 10. The variables most affecting KAP of the people on environmental health were environmental health education(r=0.2980) and education(r=0.2419) and the next were age, marital status, religion, electronic media, print media, place of birth and gender. 11. The most important variable affecting KAP level of the people on environmental health was education level.

• Korean Journal of Health Education and Promotion
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• v.6 no.2
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• pp.4-22
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• 1989

This study aims to explore the relationship between housewives' health KAP level and the physical health of families. The data used in this study are obtained from the Last Evaluation Program of the National Note for Health surveyed in July, 1989. The respondents for this study are 770 housewives residing in Chunan and Buan. The reason why this study focus on KAP level of housewives is to find out whether a housewife as a emotional supporter contributes to the physical health of her families. A housewife gives her families emotional satisfaction insteade of economic satisfaction. So she has the most interest in family health among the members of her family. Therefore, housewife's KAP level will influence her family health. The independent variables chosen for the analysis are the general characteristic variables and KAP level. And the dependent variable is the physical health of families which excluded psychosocial one. This level of family health includes weight for family health and seriouseness of disease. The result of this study was summarized as follows. (1) KAP level was significantly correlated with variables which have mainly the socioeconomic characteristics. The variables were: area of residence, education level occupation, self assessment on wealth, and exposure to mass communication. (2) In the analysis of relationship between the general characteristic variables and family health, family health was significantly correlated with almost all variables. The variables were: Presence of the aged, Health status, Experience in disease, Self assessment on health, No. of families, Occupation, Education level, Self assessment on wealth, Concern on health, and Exposure to mass communication. (3) In the analysis of relationship between family health and KAP level, family health was not significantly correlated with KAP level but. (4) Also in the stepwise regression analysis, the general variables account for about 32.1 percent of the variance in the dependent variable, family health. The variable with the greatest explanatory power was presence of the aged. On the contrary, KAP level explain about 0.4 percent of the variance in the dependent variable. In sum, the study shows that housewives' health KAP level has relatively weak relationship with the physical health of families

• The Journal of the Korea Contents Association
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• v.11 no.3
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• pp.302-308
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• 2011

The aim of this study is to compare reference levels for radiation dose in angiography and interventional radiology. Proposed reference levels for various procedures and classification of diseases are provided by fluoroscopy time and kerma area product(KAP) rate normalizing the body habitus focusing the cerebrum. Subarachnoid hemorrhage(SAH) represents the highest KAP-rates and aneurysm represents the lowest KAP-rates. According to these types of procedures, internal carotid artery(ICA), common carotid artery(CCA), and vertebral artery(VA) show the highest KAP-rates and guglielmi detachable coil shows the lowest KAP-rates. Therefore, the present study can suggested reference levels for patient radiation dose and is expected to be further useful in the field of radiation dose education and management of angiography and interventional radiology.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.3
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• pp.328-337
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• 2017

Objective: An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness. Methods: In previous work, we constructed a skin cDNA library and isolated a full-length cDNA clone termed KAP11.1. On this basis, we conducted a series of bioinformatics analysis. Tissue distribution of KAP11.1 mRNA was performed using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The expression of KAP11.1 mRNA in primary and secondary hair follicles was performed using real-time PCR (real-time polymerase chain reaction) analysis. The expression location of KAP11.1 mRNA in primary and secondary hair follicles was performed using in situ hybridization. Results: Bioinformatics analysis showed that KAP11.1 gene encodes a putative 158 amino acid protein that exhibited a high content of cysteine, serine, threonine, and valine and has a pubertal mammary gland) structural domain. Secondary structure prediction revealed a high proportion of random coils (76.73%). Semi-quantitative RT-PCR showed that KAP11.1 gene was expressed in heart, skin, and liver, but not expressed in spleen, lung and kidney. Real time PCR results showed that the expression of KAP11.1 has a higher expression in catagen than in anagen in the primary hair follicles. However, in the secondary hair follicles, KAP11.1 has a significantly higher expression in anagen than in catagen. Moreover, KAP11.1 gene has a strong expression in inner root sheath, hair matrix, and a lower expression in hair bulb. Conclusion: We conclude that KAP11.1 gene may play an important role in regulating the fiber diameter.