• Title/Summary/Keyword: KAI-1

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Development of Penicillium italicum-Specific Primers for Rapid Detection among Fungal Isolates in Citrus

  • Chen, Kai;Tian, Zhonghuan;Jiang, Fatang;Long, Chao-an
    • Journal of Microbiology and Biotechnology
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    • v.29 no.6
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    • pp.984-988
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    • 2019
  • Blue mold in citrus is caused by Penicillium italicum. In this study, the P. italicum-specific primers were developed for rapid detection based on the conserved genes RPB1 and RPB2 among Penicillium genomes. The two primer pairs RPB1-a and RPB1-b proved to be specific to detect P. italicum. The PCR assay among 39 fungal isolates and the colonial, pathogenic morphologies and molecular methods validated the specificity and reliability of these two primer pairs. This report provided a method and P. italicum-specific primers, which might greatly contribute to citrus postharvest industry.

The Role of Maternal Interpersonal Relation Satisfaction in the Relationship between Conflicted Teacher-Child Relationship and Negative Peer Interaction Quality in Young Children

  • Chung, Kai-Sook;Kim, Mina
    • International Journal of Human Ecology
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    • v.15 no.1
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    • pp.12-22
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    • 2014
  • The effects of conflicted teacher-child relation on conflicted or passive peer interaction and a moderation effect of mothers' interpersonal relation satisfaction on the associations were assessed. Children from 2- to 6-year-olds (184 girls, 185 boys) mostly from middle socioeconomic-status urban community in Korea and their teachers and mothers participated. Conflicted teacher-child relation predicted conflicted peer interaction but not passive peer interaction. Children, whose relationship with teachers were conflicted, engaged in conflicted play with peers more often than children who were in less conflicted relationship with the teachers. Teachers who were in conflicted relationship with the children, perceived the children having conflicted interaction with peers more often, if mothers of the children were less satisfying in relationship with significant others, especially boys. Children, whose mothers are in less satisfying interpersonal relation with others, were more passive in peer interaction than children whose mothers are in more satisfying interpersonal relationship.

Multi-field Coupling Simulation and Experimental Study on Transformer Vibration Caused by DC Bias

  • Wang, Jingang;Gao, Can;Duan, Xu;Mao, Kai
    • Journal of Electrical Engineering and Technology
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    • v.10 no.1
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    • pp.176-187
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    • 2015
  • DC bias will cause abnormal vibration of transformers. Aiming at such a problem, transformer vibration affected by DC bias has been studied combined with transformer core and winding vibration mechanism use multi-physical field simulation software COMSOL in this paper. Furthermore the coupling model of electromagnetic-structural force field has been established, and the variation pattern of inner flux density, distribution of mechanical stress, tension and displacement were analyzed based on the coupling model. Finally, an experiment platform has been built up which was employed to verify the correctness of model.

Novel Switched-Inductor Quasi-Z-source Inverter

  • Deng, Kai;Zheng, Jianyong;Mei, Jun
    • Journal of Power Electronics
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    • v.14 no.1
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    • pp.11-21
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    • 2014
  • A novel switched-inductor quasi-Z-source inverter is proposed in this study. Compared with classic topologies, the boost ability of the proposed topology is strengthened. The voltage stress of the capacitors, diodes, and power devices are reduced, and the current ripple of the DC voltage source is suppressed. Conversion efficiency is also improved. The operation principle of the proposed topology is analyzed in detail and compared with that of similar topologies. The feasibility of the proposed topology is verified by simulations and experiments on a laboratory prototype.

Novel Filtering Power Divider with External Isolation Resistors

  • Lu, Yun-Long;Wang, Shun;Dai, Gao-Le;Li, Kai
    • ETRI Journal
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    • v.37 no.1
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    • pp.61-65
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    • 2015
  • In this paper, a novel filtering power divider with external isolation resistors is presented. The proposed power divider can be considered as an integration of a bandpass filter and a Gysel power divider. Based on the circuit topology, a high-order filtering power divider can be easily realized. Odd- and even-mode models are employed to analyze the filtering and power splitting functions. For demonstration, a third-order filtering power divider operating at 1.5 GHz is designed and implemented. The measured results exhibit an isolation between the output ports that is better than 20 dB at around the center frequency.

The Effect of the Korean Wave on Malaysian University Students' Perception

  • Lee, Sang-Hyeop;Lee, Kai-Sean;Kwak, Gong-Ho;Kim, Hak-Seon
    • Culinary science and hospitality research
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    • v.23 no.1
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    • pp.79-83
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    • 2017
  • This paper attempted to understand the university students' perception on the Korean Wave in Malaysia. Six Malaysian students were involved in this study and three research questions guided the entire study. The first question explored Malaysian university students' perception about the Korean Wave in general; the second involved how the media influences the perception of the Korean Wave; and third question looked into the economic effects that would influence students' perception of the Korean Wave. Among the six students, all of them had positive perception of the Korean Wave and were heavily influenced through the media. Thus, it can be said that it brought positive feedback to the Korean Wave. The awareness of the Korean Wave was found to be increasingly popular due to media development. This study helped understand the Korean Wave from Malaysian students' perception.

Partial Conductivity of YSZ Doped with 10 mol% $TiO_2$

  • Kobayashi, Kiyoshi;Kai, Yukiharu;Yamaguchi, Shu;Kawashima, Tsuyoshi;Iguchi, Yoshiaki
    • The Korean Journal of Ceramics
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    • v.4 no.2
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    • pp.114-121
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    • 1998
  • Using Hebb-Wagner's asymmetric cell, partial conductivities of holes and electrons in yttria stabilized zirconia doped with 10 mol% TiO2 have been estimated by a dc polarization measurement. The current interrruption method and ac impedance measurements have been also made to evaluate the ionic conductivity and to examine the consistency of the partial conductivities. Partial conductivities of electrons(σn) and holes (σp) were found to be pro-peortional to -1/4 and 1/4 power of partial pressure of oxygen gas, respectively, except for σn at reducing conditions. In comparison with 5 mol% doped YSZ, σn was found to increase with the increase of TiO2 concentration, but σp stayed at almost a constant value.

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ON THE NORM OF THE OPERATOR aI + bH ON Lp(ℝ)

  • Ding, Yong;Grafakos, Loukas;Zhu, Kai
    • Bulletin of the Korean Mathematical Society
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    • v.55 no.4
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    • pp.1209-1219
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    • 2018
  • We provide a direct proof of the following theorem of Kalton, Hollenbeck, and Verbitsky [7]: let H be the Hilbert transform and let a, b be real constants. Then for 1 < p < ${\infty}$ the norm of the operator aI + bH from $L^p(\mathbb{R})$ to $L^p(\mathbb{R})$ is equal to $$\({\max_{x{\in}{\mathbb{R}}}}{\frac{{\mid}ax-b+(bx+a){\tan}{\frac{\pi}{2p}}{\mid}^p+{\mid}ax-b-(bx+a){\tan}{\frac{\pi}{2p}}{\mid}^p}{{\mid}x+{\tan}{\frac{\pi}{2p}}{\mid}^p+{\mid}x-{\tan}{\frac{\pi}{2p}}{\mid}^p}}\)^{\frac{1}{p}}$$. Our proof avoids passing through the analogous result for the conjugate function on the circle, as in [7], and is given directly on the line. We also provide new approximate extremals for aI + bH in the case p > 2.

Identification of an Antifungal Chitinase from a Potential Biocontrol Agent, Bacillus cereus 28-9

  • Huang, Chien-Jui;Wang, Tang-Kai;Chung, Shu-Chun;Chen, Chao-Ying
    • BMB Reports
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    • v.38 no.1
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    • pp.82-88
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    • 2005
  • Bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in Taiwan. This bacterium exhibited biocontrol potential on Botrytis leaf blight of lily as demonstrated by a detached leaf assay and dual culture assay. At least two chitinases (ChiCW and ChiCH) were excreted by B. cereus 28-9. The ChiCW-encoding gene was cloned and moderately expressed in Escherichia coli DH5$\alpha$. Near homogenous ChiCW was obtained from the periplasmic fraction of E. coli cells harboring chiCW by a purification procedure. An in vitro assay showed that the purified ChiCW had inhibitory activity on conidial germination of Botrytis elliptica, a major fungal pathogen of lily leaf blight.

Single-base Discrimination Mediated by Proofreading Inert Allele Specific Primers

  • Lin-Ling, Chen;Zhang, Jia;Sommer, Steve S.;Li, Kai
    • BMB Reports
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    • v.38 no.1
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    • pp.24-27
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    • 2005
  • The role of 3' exonuclease excision in DNA polymerization was evaluated for primer extension using inert allele specific primers with exonuclease-digestible ddNMP at their 3' termini. Efficient primer extension was observed in amplicons where the inert allele specific primers and their corresponding templates were mismatched. However, no primer-extended products were yielded by matched amplicons with inert primers. As a control, polymerase without proofreading activity failed to yield primer extended products from inert primers regardless of whether the primers and templates were matched or mismatched. These data indicated that activation was undertaken for the inert allele specific primers through mismatch proofreading. Complementary to our previously developed SNP-operated on/off switch, in which DNA polymerization only occurs in matched amplicon, this new mutation detection assay mediated by $exo^+$ DNA polymerases has immediate applications in SNP analysis independently or in combination of the two assays.