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Detection of the Carcinogenic Liver Fluke Opisthorchis viverrini Using a Mini Parasep SF Faecal Parasite Concentrator

  • Kaewpitoon, Soraya J;Rujirakul, Ratana;Tongtawee, Taweesak;Matrakul, Likit;Panpimanmas, Sukij;Wakkuwattapong, Parichart;Loyd, Ryan A;Kaewpitoon, Natthawut
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.1
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    • pp.373-376
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    • 2016
  • The Mini Parasep SF fecal parasite concentrator (MPSFC) is a new modification of the closed concentration system, which can easily be adopted in any routine clinical pathology laboratory. Here we describe our experience with the system in diagnosing Opisthorchis viverrini. A total of 199 fecal samples was submitted for routine examination in the clinical pathology laboratory of Suranaree University of Technology hospital, Nakhon Ratchasima province, Thailand, during August to October 2015. Out of all samples examined, 10 (5.03%) were positive with intestinal parasites including O. viverrini (2.01%), followed by Strongyloided stercoralis (1.51%), Hookworm (0.5%), Taenia spp. (0.5%), and Entamoeba coli (0.5%). Regarding the distribution of intestinal parasites in relation to the methods used, and found that 4 samples (2.01%) were positive using the direct wet smear method while 10 (5.03%) were positive with the Mini Parasep SF method; the difference was statistically significant ($X^2$-test = 116.506, p-value =0.001). Mean time for processing using the Parasep system was 6.03 min/sample, the conventional direct wet smear method at 0.3 min/sample. Cost per test, conventional direct wet smear method costing less than the Parasep method at USD 0.74/sample versus USD 1.47/sample. This first report of O. viverrini detection using MPSFC indicates that Parasep concentration test is useful in the routine laboratory, increasing the yield of parasites as compared to direct microscopy, but with greater processing time and cost. Further comparisons between the Parasep concentration test and common methods for O. viverrini detection are required, particularly concerning use in epidemiological surveys.

Effect of Carbides on the Tensile Properties of 0.5C-17Cr-0.5Ni Martensitic Stainless Steel (0.5C-17Cr-0.5Ni 마르텐사이트계 스텐인리스강의 인장성질에 미치는 탄화물의 영향)

  • Kwon, Soon-Doo;Son, Dong-Wook;Kang, Chang-Yong
    • Journal of Power System Engineering
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    • v.20 no.3
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    • pp.11-16
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    • 2016
  • The effect of carbides on the tensile properties in 0.5C-17Cr-0.5Ni martensitic stainless steel was studied. With the increase of austenitizing temperature, the volume fraction of residual carbide was decreased rapidly. In tempered specimens after quenching, the volume fraction of total carbide was decreased with the increase of austenitizing temperature. In tempered specimens after quenching, strength was decrease and elongation was increased with the increase of austenitizing temperature. Tensile strength was increase and elongation was decreased with the increase of volume fraction of residual and total carbides. With the increase of austenitizing temperature, the tensile properties of mod. 0.5C-17Cr-0.5Ni martensitic stainless was affected greatly by residual carbide than tempered carbide.

Effects of Soil pH on the Growth and Antioxidant System in French Marigold (Tagetes patula L.) (토양 pH가 만수국(Tagetes patula L.)의 생육 및 항산화 작용에 미치는 영향)

  • Kim, Jeung-Bea;Cho, Hyun-Je;Kim, Hak-Yoon
    • Korean Journal of Plant Resources
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    • v.20 no.4
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    • pp.348-352
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    • 2007
  • To investigate the effects of soil pH on plants, the seedlings of french marigold (Tagetes patula L.) was transplanted into the soils acidified with $H_{2}SO_{4}$ solutions (pH 5.3, 4.5, 3.9, 3.5). The level of malondialdehyde was significantly increased by soil acidification. As the pH levels decreased from 5.3 to 3.5, the contents of dehydroascorbate and oxidized glutathione of the plant were significantly increased. The antioxidative enzyme activities of the plant affected by soil acidification were increased as the pH decreased.

Clinical Observation of Effect on Severity of Symptoms and Safety of Auto-microneedle Therapy in Patients with Peripheral Facial Paralysis (자동 미세침이 말초성 안면마비 환자의 증상 정도 및 안전성에 미치는 영향에 대한 임상 관찰)

  • Lee, Ung-In;Kwon, You-Jung;Kim, Hyun-Ho;Yoo, Je-Hyuk;Kim, Kyung-Wook;Kang, Jung-Won;Lee, Sang-Hoon
    • Journal of Acupuncture Research
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    • v.29 no.4
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    • pp.35-42
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    • 2012
  • Objectives : This study was to observe clinical application of auto-microneedle therapy system (AMTS) in patients with peripheral facial paralysis. Methods : 27 peripheral facial paralysis patients were observed after taking AMTS at Facial Palsy Center, Kyung Hee University Oriental Medicine Hospital from March 1, 2011 to January 9, 2012. We assessed the symptoms of facial paralysis with Yanagihara unweighted grading system, Sunnybrook facial grading system(SBGS) and facial disability index(FDI), and observed adverse events and total safety of the treatment. Results : The scores of facial palsy scales increased after AMTS in Yanagihara grading system and Sunnybrook facial grading system. AMTS-related adverse events were mild pain(5.9%) and fatigue(3.5%), which needed no extra treatment. The total safety evaluation was between 'safe' and 'nearly safe' level. There were no other serious adverse events. In addition, patients were satisfied with subjective improvement including facial tingling and numbness. Conclusions : AMTS can be applied as an adjunctive treatment for patients with peripheral facial paralysis due to its safety and clinical usefulness. It is easier to stimulate wide skin area in a short time. Further clinical research is required to investigate the effectiveness of ATMS in a more rigorous RCTs.

Heat Transfer and Total Friction Factors in the Convergent Channels with V/⋀-shaped Ribs on Two Opposite Walls (양 벽면에 V/⋀형 리브가 있는 수축 채널의 열전달과 전 마찰계수)

  • Lee, Myung-Sung;Heo, Meo-Seong;Jeong, Ui-Jae;Park, Young-Joon;Yoo, Jung-Hyun;Im, Gun-Woo;Ahn, Soo-Whan
    • Journal of Power System Engineering
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    • v.20 no.5
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    • pp.66-71
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    • 2016
  • The measurements of heat transfer and total friction factors for turbulent flows in the convergent rectangular channels with two opposite in-line ribbed walls are reported. The study has covered three different angled ribs ($30^{\circ}$, $45^{\circ}$ and $60^{\circ}$) and Reynolds number in the range of 22,000 to 75,000. The channel, composing of ten isolated copper sections in the length of test section of 1 m, has the channel convergence ratio of $D_{ho}/D_{hi}=0.67$. The results show that the ribs pointing downstream (${\wedge}-shaped$) is somewhat greater than the ribs pointing upstream (V-shaped) in the dimensionless Nusselt number and total friction factors.

Mechanical Evaluation of Posterior Dynamic Omega-wire Stabilization System (후방 요추 극돌기간 유동적 오메가형 스프링 고정재의 역학적 평가)

  • Lee, Yeon-Soo;Song, Geun-Soo
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.36 no.11
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    • pp.1099-1104
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    • 2012
  • The study investigates the mechanical deformation of a newly developed screwless omega-wire dynamic system for stabilization of the spine. The omega-wire spring stabilization system was tested under tension, compression, and dynamic compressive fatigue loads. In addition, its bending deformation was compared to that of a spiral-wire spring system using FEA. A model whose hanger inter-center distance is 60 mm showed an ultimate tensile stress of 3981.7 N at a displacement of 3.61 mm and an ultimate compressive load of 535.6 N at a displacement of 2.16 mm. Under fatigue loading of 5 Hz with 10 N/1 N, it did not show any failure over 5 million cycles, and the displacement was restricted to 8-9 mm. In the FEA, the omega-wire spring system showed more flexible bending features than did the spiral-wire spring system.

Large Eddy Simulation of Fire and Smoke Control in a Compartment with Large Openings (큰 개구부가 있는 공간의 화재와 제연의 대와류모사)

  • 박외철
    • Fire Science and Engineering
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    • v.17 no.3
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    • pp.7-12
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    • 2003
  • A 50 kW polyurethane fire in a compartment of 4 m ${\times}$ 1 m ${\times}$ 1.5m with large openings similar to a subway station was simulated by a large eddy simulation to investigate the fire and smoke control. The NIST FDS, which employed a mixture fraction combustion model and a finite volume method for radiation, was utilized. Distribution of temperature and smoke particles was compared with in the lower and upper corridors for three different smoke control systems, ventilation, purge, and extraction, starting in 5 sec from the ignition of the fire. For the given geometries, the ventilation system showed the best smoke removal rate and lowest temperature distribution in the both corridors. It was confirmed that the purge system is not recommended for a subway station since the smoke removal rate of the purge system was worse than that without a smoke control system.

New K-Ar dating system in Korea Basic Science Institute: Summary and Performance (한국기초과학지원연구원에 도입된 K-Ar 연대 측정시스템: 개요 및 성능)

  • 김정민
    • The Journal of the Petrological Society of Korea
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    • v.10 no.3
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    • pp.172-178
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    • 2001
  • K-Ar dating system of Korea Basic Science Institute (KBSI) was installed in 1997 and has been used since then. The system consists of high temperature graphite furnace, gas purification system, and mass spectrometer with data acquisition system. K-Ar age is determined by the measurement of the concentrations of Ar and K through isotope dilution method using $^{38}Ar$ as spike and flame spectroscopy, respectively. The accuracy and reliability for the K-Ar age are checked using the several K-Ar standard materials. Although the exact age determination for young samples of less than 1 Ma is hampered by small fluctuations of sensitivity and mass discrimination, the present system yields the reliable K-Ar age compared to the standard materials of Tertiary and Mesozoic age. The measurements for the SORI93 biotite with the recommended K-Ar age of $92.6\pm$0.6 Ma and Bern4M muscovite of $18.5\pm$0.6 Ma yield the reliable age of $92.1\pm$1.1 Ma and $17.8\pm$0.2 Ma, respectively.

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Evolutionary game theory-based power control for uplink NOMA

  • Riaz, Sidra;Kim, Jihwan;Park, Unsang
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.12 no.6
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    • pp.2697-2710
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    • 2018
  • Owing to the development of Internet of Things (IoT), the fifth-generation (5G) wireless communication is going to foresee a substantial increase of mobile traffic demand. Energy efficiency and spectral efficiency are the challenges in a 5G network. Non-orthogonal multiple access (NOMA) is a promising technique to increase the system efficiency by adaptive power control (PC) in a 5G network. This paper proposes an efficient PC scheme based on evolutionary game theory (EGT) model for uplink power-domain NOMA system. The proposed PC scheme allows users to adaptively adjusts their transmit power level in order to improve their payoffs or throughput which results in an increase of the system efficiency. In order to separate the user signals, a successive interference cancellation (SIC) receiver installed at the base station (BS) site. The simulation results demonstrate that the proposed EGT-based PC scheme outperforms the traditional game theory-based PC schemes and orthogonal multiple access (OMA) in terms of energy efficiency and spectral efficiency.

Cloning and Expression of the Rhodobacter capsulatus hemA Gene in E. coli for the Production of S-Aminolevulinic Acid

  • KANG , DAE-KYUNG;KIM, SANG-SUK;CHI, WON-JAE;HONG, SOON-KWANG;KIM, HA-KUN;KIM, HYUN-UK
    • Journal of Microbiology and Biotechnology
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    • v.14 no.6
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    • pp.1327-1332
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    • 2004
  • The hemA gene encoding 5-aminolevulinic acid synthase (ALAS) was cloned from Rhodobacter capsulatus, and its nucleotide sequence was determined. DNA sequencing data revealed one open reading frame coding for a protein with 401 amino acids that displayed high similarity to the amino acid sequences of other known ALASs. The hemA gene was then cloned and expressed under the control of constitutive promotor in E. coli. The recombinant E. coli strain was able to accumulate 5-aminolevulinic acid to 21 mM in the liquid medium supplemented with 45 mM glycine and 120 mM succinate. In addition, a marked effect of the pH of the culture medium on ALA production was observed, and the optimum pH for culture medium was determined to be 5.8-6.3.