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Immobilization of Lipases on Amberlite and Their Interesterification Reaction Characteristics (Amberlite에 고정화된 Lipase 제조 및 효소적 Interesterification을 이용한 반응 특성 연구)

  • Park, So Ra;Lee, Ki Teak
    • Korean Journal of Food Science and Technology
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    • v.46 no.3
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    • pp.315-322
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    • 2014
  • Immobilized lipases were prepared by physical adsorption using lipase AK, AY, AH, PS and R on Amberlite$^{(R)}$XAD$^{(R)}$7 HP resin. With the immobilized lipases (10%), structured lipid was synthesized by enzymatic interesterification of canola oil, palmitic ethyl ester, and stearic ethyl ester in order to study the reaction characteristics. Among the lipase, the highest protein content was obtained from lipase AH (11.41%) before immobilization, while the highest levels of bound protein was observed from immobilized lipase AK (63.91%). Immobilized lipase AK had the highest interesterification activity (38.3% of total saturated fatty acid). Lipase AK was also used for a continuous reaction in which the slow flow of reactant resulted in increased reaction rate. Reusability of immobilized AK, AH and PS increased at the second reaction (120-196.5%). However, the activity of immobilized AK, which had the highest bound protein content (63.91%) decreased after the third reaction, while the activity of immobilized AH and PS was maintained until the sixth reaction.

Anti-microbial Activity of Grapefruit Seed Extract and Processed Sulfur Solution against Human Skin Pathogens (피부질환 원인균에 대한 자몽종자추출물과 법제유황수의 항균 효과)

  • Ha, Yu-Mi;Lee, Bo-Bae;Bae, Hee-Jung;Je, Kyoung-Mo;Kim, Soon-Rae;Choi, Jae-Suk;Choi, In-Soon
    • Journal of Life Science
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    • v.19 no.1
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    • pp.94-100
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    • 2009
  • This study was carried out to examine the antimicrobial effects of grapefruit seed extract (GSE) and processed sulfur solution (PSS) against human skin pathogens: Malassezia furfur, M. restricta, Propionibacterium arnes, Trichophyton mentagrophytes and T. rubrum. The antimicrobial effects of GSE and PSS were tested by agar diffusion method and micro broth dilution method. As the results, the MIC values of GSE against M. furfur, M. restricta, P. acnes, T. mentagrophytes and T. rubrum were 3.91, 3.91, 0.004, 0.024, and $0.012{\mu}l/ml$, respectively. The MIC values of PSS were 0.03, 0.03, 0.156, 0.003, and $0.012{\mu}l/ml$, respectively. Antimicrobial activity of skin care emulsion products containing 0.5% GSE and 0.5% PSS against human skin pathogens were 5.2, 4.3, 8.0, 9.5 and 12.8 mm, respectively. Refractive index, pH, viscosity and color value of skin care emulsions containing GSE and PSS were measured. According to these results, it was concluded that the GSE and PSS were the promising sources of antibacterial agent which could be useful for skin and hair care products as well as for the alternative medicine development in treatment of certain types of skin ailments.

Effects of High Hydrostatic Pressure on the Shelf-life and Quality of Dongchimi (초고압처리 동치미의 저장 안정성 및 품질 특성)

  • Hong, Kwan-Pyo;Park, Ji-Yong
    • Korean Journal of Food Science and Technology
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    • v.30 no.3
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    • pp.602-607
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    • 1998
  • Changes in microbial count, enzyme, texture and color during storage of dongchimi (pickled radish roots) were investigated. Dongchimi was pressurized at 400 MPa for 5 min. Pressurized dongchimi stored at $4^{\circ}C\;(PS4)\;and\;37^{\circ}C\;(PS37)$ were compared with control $(stored\;at\;4^{\circ}C)$. PS4 and PS37 maintained their initial pH values during storage. Lactic acid bacteria, yeast and mold were not detected during storage in pressurized dongchimi. Pectinesterase, polygalacturonase activities and hardness of pressurized dongchimi decreased during storage. PS4 maintained its hardness longer than both control and PS37 during storage. The yellowness of PS37 was too high compared to control and PS4, indicating that storage of pressurized dongchimi at $37^{\circ}C$ was undesirable.

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Effects of YkJungJiHwangTang(YJJHT) on Inhibition of Impairment of Learning and Memory, and Acetylcholinesterase in Amnesia Mice (익정지황탕(益精地黃湯)이 치매병태(痴寐病態)모델에 미치는 영향(影響))

  • Choi Byong-Man;Lee Sang-Ryong
    • Journal of Oriental Neuropsychiatry
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    • v.11 no.2
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    • pp.23-42
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    • 2000
  • Alzheimer's disease(AD) is progressive neurodegenerative disease, which is pathologically characterized by neuritic plaques and neurofibrillary tangles associated with the acetylcholinesterase, apolipoprotein E and butylcholinesterase, and by mutations in the presenilin genes PSI and PS2, and amyloid precursor proteins (APP) overexpression. The present research is to examine the inhibition effect of YJJHT on PS-1, PS-2 and APP overexpression by detected to Western blotting. To verify the EFFects of YJJHT on cognitive deficits further, we tested it on the scopolamine-induced amnesia model of the mice using the Morris water maze tests. and there was ameliorative effects of memory impairment s a protection to scopolamine. YJJHT only partially blocked the increase in blood serum level of acetylcholinesterase and Uric acid induced by scopolamine. whereas blood glucose level was shown to attenuate the amnesia induced by scopolamine and inreased extracellular serum level compared with only scopolamine injection. In conclusion, studies of YJJHT that has been know as anti-choline and inhibition ablilities of APP overexpression, this could also be used further as a important research data for a preventive and promising symptomatic treatment for Alzheimer's disease.

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Appropriateness Evaluation of Plug Seedling Cultivation of Rehmannia glutinosa (지황의 플러그 육묘재배 적정성 평가)

  • Lee, Sang-Hoon;Lee, Yoon-Jeong;Oh, Myeong-Won;Lee, So-Hee;Koo, Sung-Cheol;Hur, Mok;Lee, Woo-Moon;Chang, Jae-Ki;Kim, Ei-Hyun;Han, Jong-Won
    • Korean Journal of Plant Resources
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    • v.33 no.2
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    • pp.73-79
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    • 2020
  • This study was conducted to evaluate the appropriateness cultivation of R. glutinosa by plug seedling. Cultivation by direct seeding (DS) and plug seedling (PS) of R. glutinosa was conducted in each of the Pyeongchang and Eumseong areas. As a result, locally, for the Eumseong area, aerial parts in leaf length, leaf width and number of leaves per plant were better than for the Pyeongchang area. In the cultivation method, leaf width and number of leaves per plant were better with PS cultivation than with DS cultivation. Locally, rhizome yield of underground parts in the Eumseong area was better than that in the Pyeongchang area, but the rhizome yield with DS cultivation in the Eumseong area and PS cultivation in the Pyeongchang area were statistically the same. The fresh rhizome yield for DS in Eumseong, PS in Eumseong, DS in Pyeongchang and PS in Pyeongchang was 1,253 kg/10a, 1,376 kg/10a, 923 kg/10a and 1,256 kg/10a, respectively. Finally, the catalpol content for DS in Eumseong, PS in Eumseong, DS in Pyeongchang and PS in Pyeongchang was 3.67%, 2.03%, 2.96% and 1.60%, respectively. From these results, it can be seen that the R. glutinosa PS cultivation method can increase the rhizome yield.

Effect of Surfactant on Rheological and Electrical Properties of Latex-Blended Polystyrene/Single-Walled Carbon Nanotube Nanocomposites (계면활성제가 라텍스 블렌딩 폴리스티렌/단일벽 탄소나노튜브 나노복합재료의 유변학적, 전기적 물성에 미치는 영향)

  • Kang, Myung-Hwan;Noh, Won-Jin;Woo, Dong-Kyun;Lee, Seong-Jae
    • Polymer(Korea)
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    • v.36 no.3
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    • pp.364-371
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    • 2012
  • Polystyrene/single-walled carbon nanotube (PS/SWCNT) nanocomposites were prepared by latex technology and the effect of surfactant (SDS) on nanotube dispersion, rheological and electrical properties was investigated. The nanocomposites were prepared through freeze-drying after mixing PS particles and aqueous SWCNT/SDS suspension. As the SDS content increased, the storage modulus and complex viscosity of the nanocomposites were increased due to enhanced dispersion of nanotubes, but if the content excessively increased, the modulus and viscosity began to decrease due to low molecular weight of SDS. The electrical conductivity sharply increased with the addition of SDS, and then did not show significant changes. This result is speculated to be the competition between the increased dispersion of nanotubes and the deterioration of electrical conductivity by SDS adsorption. An optimal ratio of SDS to SWCNT for improving electrical conductivity and end-use properties was 2. With this ratio, the electrical percolation threshold of SWCNT was less than 1 wt%.

Oxidative Stability of Peanut (Arachis hypogaea L.) Oil and Quality Characteristics of the Roasted Peanuts (볶음땅콩의 품질특성과 땅콩기름의 산화안정성)

  • Park, Bock-Hee;Lee, Jung-Hee;Kim, Sun-Hee;Cho, Hee-Sook
    • Journal of the Korean Society of Food Culture
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    • v.32 no.2
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    • pp.128-134
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    • 2017
  • The purpose of this study was to investigate the quality characteristics of roasted peanuts and the antioxidative effect of peanut oil added with sesame oil. Saltiness of roasted peanut increased with increasing salt content. In terms of color value, L and b values decreased as increasing concentration increased, whereas a value increased with increasing salt content. Overall, sensory evaluations proved that roasted peanuts with 15% added salt was preferred over other samples. For peanut oil added with sesame oil, acid values increased during the storage period, whereas samples made with sesame oil had lower values than the control group. Peroxide values increased rapidly for 21 days and then decreased. The acid and peroxide values were lower in peanut oil added with 50% sesame oil compared to peanut oil added with 30%, 10%, and 70% sesame oil, as well as the control. The TBA values of peanut oil made with 50% and 30% sesame oil were lower than those of the control and 70% and 10% sesame oil. According to the Rancimat method, PS-50% (524 min) and PS-30% (453 min) demonstrated longer induction periods as compared to the control (280 min), PS-70% (445 min), and PS-10% (291 min) samples.

Development of Rotary Cover for Energy Saving used in a Farming Tractor (소요동력 절감을 위한 트랙터용 로타리 커버의 개발)

  • Oh, Jong-Jin;Kim, Ki-Dae;Lee, Hyun-Dong;Kim, Chan-Su;Kim, Sung-Hwan;Na, Kun-Young
    • Korean Journal of Agricultural Science
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    • v.29 no.2
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    • pp.67-77
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    • 2002
  • This study aims at investigating the realities of tractor rotary tilling and paving operations performed by farm families in the central part of the country, measuring the power requirement so as to find the energy-saving factors for tractor rotary tilling, and designing a device to prevent soil adherence to the inside of the rotary cover-this part is the most subject to soil adherence and affects the power requirement. As a result of rotary tilling tests using rotaries equipped with a soil adherence preventer, the power requirement values were measured to be 52.18, 52.44, 49.01 and 46.34 PS for general, stainless steel, rubber and PE rotaries, and as a results of measurement of the quantities of the soil adhered to rotaries after their tilling tests, on the average, the quantities were measured to be 5.6, 5.7, 3.1 and 2.7kg for general, stainless steel, rubber and PE rotaries, respectively. The PE rotary cover reduced the power requirement and soil adherence by 11 and 52%, respectively.

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A 2.7Gbps & 1.62Gbps Dual-Mode Clock and Data Recovery for DisplayPort in $0.18{\mu}m$ CMOS

  • Lee, Seung-Won;Kim, Tae-Ho;Lee, Suk-Won;Kang, Jin-Ku
    • Journal of IKEEE
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    • v.14 no.1
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    • pp.40-46
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    • 2010
  • This paper describes a clock and data recovery (CDR) circuit that supports dual data rates of 2.7Gbps and 1.62Gbps for DisplayPort standard. The proposed CDR has a dual mode voltage-controlled oscillator (VCO) that changes the operating frequency with a "Mode" switch control. The chip has been implemented using $0.18{\mu}m$ CMOS process. Measured results show the circuit exhibits peak-to-peak jitters of 37ps(@2.7Gbps) and 27ps(@1.62Gbps) in the recovered data. The power dissipation is 80mW at 2.7Gbps rate from a 1.8V supply.

Job Burnout, Engagement and Turnover Intention of Dietitians and Chefs at a Contract Foodservice Management Company

  • Lee Kyung-Eun;Shin Kang-Hyun
    • Journal of Community Nutrition
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    • v.7 no.2
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    • pp.100-106
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    • 2005
  • The purposes of the study were to assess levels of burnout, engagement, and turnover intention of dietitians and chefs and to investigate the relationships among the antecedents and consequences of burnout and engagement. A total of 257 dietitians and chefs at a contract foodservice management company in Korea were surveyed using a self-administered questionnaire. Burnout (exhaustion, cynicism, and professional efficacy) and engagement (vigor, dedication, and absorption) were measured with three dimensions each. The dietitians showed significantly higher exhaustion (p < .05) and significantly lower vigor and dedication than the chefs (ps < .05). The exhaustion and cynicism dimensions of burnout were negatively correlated with all three dimensions of engagement (ps < .001) and positively correlated with turnover intention (ps < .001). The professional efficacy was positively correlated with all three engagement dimensions (ps < .001), but not with turnover intent. In addition, turnover intention was positively correlated with negative affectivity and work-load and negatively correlated with vigor, dedication, and absorption. A series of hierarchical regression analyses were conducted to assess the effects of the personal (positive and negative affectivity) and situational factors (workload, interpersonal conflict) and job stresses on turnover intention. After removing the effects of the personal and situational factors, cynicism (p < .01) was the only significant predictor of turnover intention. Based on the findings, suggestions for recruiting and retaining qualified and motivated employees were provided.