• 제목/요약/키워드: K2PS

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Dynamics of the mobile insert helix in the domain III-IV of Aux/IAA17 probed by site-directed spin labeling and paramagnetic NMR spectroscopy

  • Han, Mookyoung;Suh, Jeong-Yong
    • 한국자기공명학회논문지
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    • 제19권2호
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    • pp.61-66
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    • 2015
  • The plant hormone auxin is involved in all stages of plant development. Aux/IAAs are the transcriptional repressors that bind to the Auxin Response Factors (ARFs) to regulate the gene expression upon auxin release. Aux/IAA have highly conserved C-terminal domains (domains III-IV) that mediate both homotypic and heterotypic interactions between Aux/IAA and ARF family proteins. Recent studies revealed that the conserved domains III-IV share a common ${\beta}$-grasp fold that oligomerizes in a front-to-back manner. In particular, Aux/IAA contains a mobile insert helix in the domain III-IV, whereas ARFs do not. Here, we investigated the dynamics of the insert helix using paramagnetic NMR spectroscopy. The insert helix exhibited fast motions in the ps-ns time scale from $^{15}N$ relaxation data, but the amplitude of the motion is likely limited to the local neighborhood. Our result suggests that the motion of the helix may have functional implications in protein-protein interactions for transcriptional regulations.

이동식 펠릿 제조장비 개발에 대한 연구 (Development of a Movable Pellet Manufacturing Equipment)

  • 조시기;금성민
    • 에너지공학
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    • 제24권3호
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    • pp.13-19
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    • 2015
  • 본 연구는 숲가꾸기 사업시 솎아내는 나무(소경간벌재), 벌채 수확 후 남긴 잔재물인 가지, 목재가공 후에 남긴 폐기 대상목재, 갱신지 벌채림 및 왕겨 등을 활용하여 펠릿 제조가 가능한 이동식 펠릿 제조 장비를 개발하는 것으로, 초기함수율이 약 32%인 도로변 가지치기 나무를 재료로 최종함수율이 약 10%인 펠릿을 생산할 경우 시간당 생산량은 약 309kg이며, 하루 약 8시간 작업시 약 2ton의 생산량이 가능하고, 펠릿 생산(시간당 약 309kg)에 필요한 평균소요동력은 약 122.4PS이며, 생산비용과 판매비용을 비교하면 1일 작업기준으로 약 268,000원의 수익이 예상되어 향후 사업성이 기대된다.

트랙터의 포장성능평가(圃場性能評價)를 위한 자료수집처리(資料蒐集處理) 시스템의 개발(開發) (Data Acquisition and Processing System for Tractors Field Performance)

  • 류관희;류영선;강은;박보순;장세권
    • Journal of Biosystems Engineering
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    • 제10권2호
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    • pp.19-26
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    • 1985
  • This study was carried out to develop a versatile data acquisition/processing system for overall tractor performance utilizing a NEC PC-8001 microcomputer. The data acquisition system measures drawbar pull and power, wheel torque and axle power, ground speed, wheel slip, fuel flow, and engine speed. The system stores hexadecimal data for these variables in memory. Upon completion of each test run, all hexadecimal data stored in memory are recorded on floppy disc. The data processing system reads in the data collected on floppy disc and interprete them using several graphical and statistical techniques. The system uses the same microcomputer and a dot-matrix printer. The data acquisition system has been installed on a GOLDSTAR 500 tractor (2WD, 50 ps). A field study has shown that tractor performance data can be quickly and easily collected. It also appeared that the data processing system can be used to efficiently analyze the collected data. The data acquisition system has some troublesome in mounting and handling on tractor since it uses a general-purpose computer consisting of several components.

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1.8-GHz 주파수 합성기용 가상 2단 링 CMOS VCO의 설계 및 분석 (A design and analysis of Pseudo 2-stage ring CMOS VCO for 1.8-GHz Frequency Synthesizer)

  • 이순섭;김세엽;남기현;조경선;갈창룡;김수원
    • 전자공학회논문지SC
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    • 제38권6호
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    • pp.48-55
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    • 2001
  • 본 논문에서 고속 가상 2단 링VCO를 내장한 1.8 GHz 주파수 합성기를 0.6m CMOS 기술을 이용하여 구현하였다. 고속 동작을 위한 링 발진기의 발진 조건을 제시하고, 출력 부하를 줄인 가상 2단 링 형태의 발진기를 제안하여 최고 1.87 GHz의 발진을 확인하였다. 이는 기존의 4단 링 VOC에 비해 21.3%의 속도가 향상된 것이다. 제안된 VCO와 함께 집적된 주파수 합성기는 위상 고정시 24ps 지터가 측정되어 고속 주파수 합성기나 고속 클럭 발진기 등에 응용이 가능함을 입증하였다.

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The Protective and Inhibitory Effect of Antioxidants Found in Broussonetia kazinoki Siebold against Oxidative DNA Damage

  • Jang, Tae-Won;Choi, Ji-Soo;Kim, Hoi-Ki;Lee, Eun-Ja;Lee, Ki-Beom;Kwon, Tae-Hyung;Kim, Do-Wan;Ahn, Jeong-Jwa;Park, Jae-Ho
    • 한국자원식물학회지
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    • 제32권6호
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    • pp.714-722
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    • 2019
  • Oxidative DNA damage negatively affects humans and the research is currently ongoing to find ways to reduce oxidative stress. Oxidative stress has been identified as a key factor in triggering various diseases. Thus, its alleviation is important for human health. Broussonetia kazinoki (B. kazinoki) has been used in traditional Korean medicine as a dermatological therapy to treat burns, pruritus, and acne. B. kazinoki is generally segregated into peeled root (PR), root bark (RB), peeled stem (PS), and stem bark (SB). To assess these components for their antioxidant activity and protection against DNA damage, their ethyl acetate fractions were examined by 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay. As a result of confirming the expression of factors involved in attenuating DNA damage, the protective effect of SB on oxidative stress suppressed the expression of p-p53 and γ-H2AX. Additionally, the levels of p53 and H2AX mRNA were significantly downregulated. In conclusion, these results indicated that the SB component of B. kazinoki had the potential to be used as an effective natural antioxidant compared to the other parts of the plant.

HD급 멀티미디어 Streaming을 위한 DSP 플랫폼 (A DSP Platform for the HD Multimedia Streaming)

  • 홍근표;박종순;문재필;김동환;장태규
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2005년도 추계종합학술대회
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    • pp.569-572
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    • 2005
  • This paper proposed the design and implementation of a DSP platform for the various multimedia streaming. The DSP platform synchronizes with host PC to configure DSP and to transmit multimedia streaming through PCI. The suggested DSP platform decodes high-capacity video/audio data using the suggested high-speed FIFO, CPLD and memory interface. The buffer control techniques is proposed in other to avoid the under/over-run of the audio/video data during the audio/video decoding. For the DSP platform test, host PC transmits program stream(PS) that consists of the MPEG-2 video MP@ML and 5.1ch AC3 audio data (Coyote.mov file, half hour running time) to DSP platform. The DSP platform plays continuously back the high sound-quality audio and high-definition video at once.

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수도 포장진입로의 설계기준에 관한 연구 (A Study on Design Standard for Paddy Field Ramp)

  • 김병갑;이중용;신승엽;김학주;이용복
    • Journal of Biosystems Engineering
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    • 제26권6호
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    • pp.517-524
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    • 2001
  • Steep and narrow ramp installed in the paddy field makes it difficult and sometimes dangerous to drive agricultural machines on it. Because agricultural machinery become larger and heavier, it is necessary to revise design standard for ramp to provide safe travel. This study was conducted to revise the design standard fur paddy field ramp, especially for its width and slope. A mathematical model predicting travelling path of a tractor on paddy field ramp and farm road was developed and simulated. To verify this model, field tests were carried out. The model could predict trvelling path with RMS Error of 12.5cm and 8.2cm, which were judged to be adoptable fur determining the width of paddy field ramp. By applying this model to 110ps tractor, which was assumed to be the largest tractor in Korea, width of paddy field ramp was determined as over 3.6m. The slope of paddy field ramp, which provided tractor with 10% of the total weight of the tractor and the mounted implement as reaction force at front wheel against ground was analyzed by considering weight transfer effect on a ramp. The result showed that adequate slope of paddy field ramp was 14°.

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Biochemical Properties and Substrate Specificity of Lipase from Staphylococcus aureus B56

  • Jung, Woo-Hyuk;Kim, Hyung-Kwoun;Lee, Chan-Yong;Oh, Tae-Kwang
    • Journal of Microbiology and Biotechnology
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    • 제12권1호
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    • pp.25-30
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    • 2002
  • A lipase of Staphylococcus aureus B56 was purified from a culture supernatant and its molecular weight was estimated to be 45 kDa by SDS-PAGE. The optimum temperature and pH for the hydrolysis of olive oil was $42^{\circ}C$ and pH 8-8.5, respectively. The enzyme was stable up to $55^{\circ}C$ in the presence of $Ca^++$ at pHs 5-11. The lipase gene was cloned using the PCR amplification method. The sequence analysis showed an open reading frame of 2,076 bp, which encoded a preproenzyme of 691 amino acids. The preproenzyme was composed of a signal sequence (37 aa), propeptide (255 aa), and mature enzyme (399 aa). Based on a sequence comparison, lipase B56 constituted of a separate subgroup among the staphylococcal lipase groups, such as S. aureus PS54 and S. haemolyticus L62 lipases, and was distinct from other lipases in their optimum pH and substrate specificity.

Cascaded Multi-Level Inverter Based IPT Systems for High Power Applications

  • Li, Yong;Mai, Ruikun;Yang, Mingkai;He, Zhengyou
    • Journal of Power Electronics
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    • 제15권6호
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    • pp.1508-1516
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    • 2015
  • A single phase H-bridge inverter is employed in conventional Inductive Power Transfer (IPT) systems as the primary side power supply. These systems may not be suitable for some high power applications, due to the constraints of the power electronic devices and the cost. A high-frequency cascaded multi-level inverter employed in IPT systems, which is suitable for high power applications, is presented in this paper. The Phase Shift Pulse Width Modulation (PS-PWM) method is proposed to realize power regulation and selective harmonic elimination. Explicit solutions against phase shift angle and pulse width are given according to the constraints of the selective harmonic elimination equation and the required voltage to avoid solving non-linear transcendental equations. The validity of the proposed control approach is verified by the experimental results obtained with a 2kW prototype system. This approach is expected to be useful for high power IPT applications, and the output power of each H-bridge unit is identical by the proposed approach.

초고속 OTDM/WDM을 위한 파장 및 반복율 가변 광 펄스 발생 (Wavelength and Repetition-Rate Tunable Optical Pulse Generation for Ultrafast OTDM/WDM)

  • 최경선;한종민;서동선;전영민;이석
    • 전기전자학회논문지
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    • 제5권2호
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    • pp.201-210
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    • 2001
  • 광 시분할 및 파장분할 다중화에 의한 초고속 광통신 시스템에 적합한 파장 및 반복율 가변 능력을 갖는 초단 펄스열을 고리형 반도체 광섬유 레이저의 광주입 모드잠김에 의해 발생시켰다. 발생된 광 펄스열은 10 GHz, 20 GHz, 30 GHz, 및 40 GHz의 다양한 반복율에서 ${\sim}10$ 피코초 정도의 펄스폭과 30 nm가 넘는 파장 가변능력을 보였다.

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