• Journal of the Korean Magnetic Resonance Society
• /
• v.19 no.2
• /
• pp.61-66
• /
• 2015

The plant hormone auxin is involved in all stages of plant development. Aux/IAAs are the transcriptional repressors that bind to the Auxin Response Factors (ARFs) to regulate the gene expression upon auxin release. Aux/IAA have highly conserved C-terminal domains (domains III-IV) that mediate both homotypic and heterotypic interactions between Aux/IAA and ARF family proteins. Recent studies revealed that the conserved domains III-IV share a common ${\beta}$-grasp fold that oligomerizes in a front-to-back manner. In particular, Aux/IAA contains a mobile insert helix in the domain III-IV, whereas ARFs do not. Here, we investigated the dynamics of the insert helix using paramagnetic NMR spectroscopy. The insert helix exhibited fast motions in the ps-ns time scale from $^{15}N$ relaxation data, but the amplitude of the motion is likely limited to the local neighborhood. Our result suggests that the motion of the helix may have functional implications in protein-protein interactions for transcriptional regulations.

• Journal of Energy Engineering
• /
• v.24 no.3
• /
• pp.13-19
• /
• 2015

The wood pellet is standardized of woody type fuel which of small cylindrical shape that is produced compress wood remnants in process of woody processing. The pellet is critical energy which expects to increase of the amount used in future. It consumes fuel which of home, common facilities stove and boiler, district heating, and CHP, etc. This study was to develop a movable pellet manufacturing equipment that can be mounted on a truck. The pellet production volume is approximately 309kg per hour, daily output is about 2ton. One days work based on the expected revenue of approximately \268,000 feasibility is considered sufficient.

• Journal of Biosystems Engineering
• /
• v.10 no.2
• /
• pp.19-26
• /
• 1985

This study was carried out to develop a versatile data acquisition/processing system for overall tractor performance utilizing a NEC PC-8001 microcomputer. The data acquisition system measures drawbar pull and power, wheel torque and axle power, ground speed, wheel slip, fuel flow, and engine speed. The system stores hexadecimal data for these variables in memory. Upon completion of each test run, all hexadecimal data stored in memory are recorded on floppy disc. The data processing system reads in the data collected on floppy disc and interprete them using several graphical and statistical techniques. The system uses the same microcomputer and a dot-matrix printer. The data acquisition system has been installed on a GOLDSTAR 500 tractor (2WD, 50 ps). A field study has shown that tractor performance data can be quickly and easily collected. It also appeared that the data processing system can be used to efficiently analyze the collected data. The data acquisition system has some troublesome in mounting and handling on tractor since it uses a general-purpose computer consisting of several components.

• Journal of the Institute of Electronics Engineers of Korea SC
• /
• v.38 no.6
• /
• pp.48-55
• /
• 2001

This paper presents a 1.8 GHz CMOS frequency synthesizer with high-speed on-chip pseudo 2-stage ring VCO. We introduce and analysis the conditions in which the ring VCO can oscillate. For high speed operation, we propose the pseudo 2-stage ring VCO that eliminates dummy loads. It can operate up to 1.87 GHz with 0.6 m CMOS process, which shows 21.3% improvement aginst the conventional 4-stage ring VCO in the aspect of the speed. When the frequency synthsizer with the psedo 2-stage ring VCO is locked at 1.85GHz, the jitter measured to 24 psec. The proposed VCO and the frequency synthesizer are directly applicable to high speed clocky synhtesizers.

• Korean Journal of Plant Resources
• /
• v.32 no.6
• /
• pp.714-722
• /
• 2019

Oxidative DNA damage negatively affects humans and the research is currently ongoing to find ways to reduce oxidative stress. Oxidative stress has been identified as a key factor in triggering various diseases. Thus, its alleviation is important for human health. Broussonetia kazinoki (B. kazinoki) has been used in traditional Korean medicine as a dermatological therapy to treat burns, pruritus, and acne. B. kazinoki is generally segregated into peeled root (PR), root bark (RB), peeled stem (PS), and stem bark (SB). To assess these components for their antioxidant activity and protection against DNA damage, their ethyl acetate fractions were examined by 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay. As a result of confirming the expression of factors involved in attenuating DNA damage, the protective effect of SB on oxidative stress suppressed the expression of p-p53 and γ-H2AX. Additionally, the levels of p53 and H2AX mRNA were significantly downregulated. In conclusion, these results indicated that the SB component of B. kazinoki had the potential to be used as an effective natural antioxidant compared to the other parts of the plant.

• Proceedings of the IEEK Conference
• /
• 2005.11a
• /
• pp.569-572
• /
• 2005

This paper proposed the design and implementation of a DSP platform for the various multimedia streaming. The DSP platform synchronizes with host PC to configure DSP and to transmit multimedia streaming through PCI. The suggested DSP platform decodes high-capacity video/audio data using the suggested high-speed FIFO, CPLD and memory interface. The buffer control techniques is proposed in other to avoid the under/over-run of the audio/video data during the audio/video decoding. For the DSP platform test, host PC transmits program stream(PS) that consists of the MPEG-2 video MP@ML and 5.1ch AC3 audio data (Coyote.mov file, half hour running time) to DSP platform. The DSP platform plays continuously back the high sound-quality audio and high-definition video at once.

• Journal of Biosystems Engineering
• /
• v.26 no.6
• /
• pp.517-524
• /
• 2001

Steep and narrow ramp installed in the paddy field makes it difficult and sometimes dangerous to drive agricultural machines on it. Because agricultural machinery become larger and heavier, it is necessary to revise design standard for ramp to provide safe travel. This study was conducted to revise the design standard fur paddy field ramp, especially for its width and slope. A mathematical model predicting travelling path of a tractor on paddy field ramp and farm road was developed and simulated. To verify this model, field tests were carried out. The model could predict trvelling path with RMS Error of 12.5cm and 8.2cm, which were judged to be adoptable fur determining the width of paddy field ramp. By applying this model to 110ps tractor, which was assumed to be the largest tractor in Korea, width of paddy field ramp was determined as over 3.6m. The slope of paddy field ramp, which provided tractor with 10% of the total weight of the tractor and the mounted implement as reaction force at front wheel against ground was analyzed by considering weight transfer effect on a ramp. The result showed that adequate slope of paddy field ramp was 14°.

• Journal of Microbiology and Biotechnology
• /
• v.12 no.1
• /
• pp.25-30
• /
• 2002

A lipase of Staphylococcus aureus B56 was purified from a culture supernatant and its molecular weight was estimated to be 45 kDa by SDS-PAGE. The optimum temperature and pH for the hydrolysis of olive oil was $42^{\circ}C$ and pH 8-8.5, respectively. The enzyme was stable up to $55^{\circ}C$ in the presence of $Ca^++$ at pHs 5-11. The lipase gene was cloned using the PCR amplification method. The sequence analysis showed an open reading frame of 2,076 bp, which encoded a preproenzyme of 691 amino acids. The preproenzyme was composed of a signal sequence (37 aa), propeptide (255 aa), and mature enzyme (399 aa). Based on a sequence comparison, lipase B56 constituted of a separate subgroup among the staphylococcal lipase groups, such as S. aureus PS54 and S. haemolyticus L62 lipases, and was distinct from other lipases in their optimum pH and substrate specificity.

• Journal of Power Electronics
• /
• v.15 no.6
• /
• pp.1508-1516
• /
• 2015

A single phase H-bridge inverter is employed in conventional Inductive Power Transfer (IPT) systems as the primary side power supply. These systems may not be suitable for some high power applications, due to the constraints of the power electronic devices and the cost. A high-frequency cascaded multi-level inverter employed in IPT systems, which is suitable for high power applications, is presented in this paper. The Phase Shift Pulse Width Modulation (PS-PWM) method is proposed to realize power regulation and selective harmonic elimination. Explicit solutions against phase shift angle and pulse width are given according to the constraints of the selective harmonic elimination equation and the required voltage to avoid solving non-linear transcendental equations. The validity of the proposed control approach is verified by the experimental results obtained with a 2kW prototype system. This approach is expected to be useful for high power IPT applications, and the output power of each H-bridge unit is identical by the proposed approach.

• Journal of IKEEE
• /
• v.5 no.2 s.9
• /
• pp.201-210
• /
• 2001

Wavelength and repetition-rate tunable optical pulse-trains for ultrafast optical time- and wavelength division multiplexing are generated from a semiconductor fiber ring laser by optical injection mode-locking. The pulse trains show the pulse with of ${\sim}10$ ps and the wavelength tuning of wider than 30 nm at various repetition-rates of 10 GHz, 20 GHz, 30 GHz and 40 GHz, respectively.