• Reproductive and Developmental Biology
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• 제34권3호
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• pp.207-211
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• 2010

Current developments in IVF and animal cloning have resulted in increasing demand for large quantities of oocytes and ovarian follicles at specific stages of development. These medical and scientific needs may be met by developing an optimal culture system for preantral follicles. In this study, we investigated the growth of porcine preantral follicle cultures in different media and in the presence and absence of serum. Follicles were manually dissected from ovaries obtained from prepubertal gilts at a local slaughterhouse, and cultured for 3 days in M199 or NCSU23 medium supplemented with porcine FSH, transferrin, L-ascorbic acid and insulin. Follicle diameters were measured on day 1 and 3 of culture. In Experiment 1, the effect of supplementing culture medium with fetal calf serum (FCS) on porcine preantral follicle growth was examined. In the group of cultures supplemented with FCS, follicle diameter after 3 days of culture, survival rate and antrum formation rate in the FCS group were significantly higher than those of the control group. In Experiment 2, the effects of culture medium (M199 and NCSU23) on follicle growth were compared. Follicle diameters were increased in the M199 group, compared with those in NCSU23 (p<0.05), but we observed no significant differences in survival and antrum formation rates between cultures grown in the two media. In conclusion, supplementation of the culture medium with serum enhances preantral follicle growth and antrum formation, and M199 is superior to NUSU23 for porcine preantral follicle culture in vitro.

• Journal of Microbiology and Biotechnology
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• 제23권4호
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• pp.539-544
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• 2013

The effects of culture depth (2-10 cm) and cell density on the growth rate and biomass productivity of Chlorella sp. XQ-200419 were investigated through the use of a self-designed open circular pond photobioreactor-imitation system. With increases in culture depths from 2 to 10 cm, the growth rate decreased significantly from 1.08 /d to 0.39 /d. However, the biomass productivity only increased slightly from 8.41 to 11.22 $g/m^2/d$. The biomass productivity (11.08 $g/m^2/d$) achieved in 4 cm culture with an initial $OD_{540}$ of 0.95 was similar to that achieved in 10 cm culture with an initial $OD_{540}$ of 0.5. In addition, the duration of maximal areal productivity at a 4 cm depth was prolonged from 1 to 4 days, a finding that was also similar to that of the culture at a 10 cm depth. In both cases, the initial areal biomass densities were identical. Based on these results and previous studies, it can be concluded that the influence of culture depth and cell density on areal biomass productivity is actually due to different areal biomass densities. Under suitable conditions, there are a range of optimal biomass densities, and areal biomass productivity reaches its maximum when the biomass density is within these optimal ranges. Otherwise, biomass productivity will decrease. Therefore, a key factor for high biomass productivity is to maintain an optimal biomass density.

• 복식문화연구
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• 제24권2호
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• pp.198-217
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• 2016

This study analyzed the effects of organizational culture on psychological empowerment and organization efficiency, and the effects of psychological empowerment on organization efficiency in fashion companies. In addition, this study analyzed the differences between organizational culture, psychological empowerment and organization efficiency across demographic characteristics. The survey was conducted among employees of fashion companies, and 320 responses were used in the data analysis. As a result, the group culture and innovative culture in fashion companies positively influenced the competence and meaning of psychological empowerment. An innovative culture and rational culture positively influenced the self-determination, and the group culture and rational culture positively influenced the impact of psychological empowerment. The group culture, innovative culture, and rational culture of fashion companies positively influenced job satisfaction, and the group culture, innovative culture, and hierarchical culture positively influenced job commitment. The group culture and innovative culture negatively influenced the turnover intention, but the hierarchical culture positively influenced the turnover intention. In addition, the psychological empowerment (competence, self-determination, impact, and meaning) positively influenced job satisfaction, and the competence, self-determination, and impact positively influenced job commitment. Competence negatively influenced the turnover intention, but the impact positively influenced the turnover intention. There was a difference in the group culture and turnover intention by ages, as well as a difference in the hierarchical culture and self-determination by work departments. The results of this study will be helpful for the establishment of the management and business strategies in fashion companies.

• Tuberculosis and Respiratory Diseases
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• 제45권2호
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• pp.271-279
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• 1998

연구배경: 결핵의 유병률은 감소하는 추세이나 노인, 항암약물요법, HIV 감염 풍에 의해 면역기능이 약화된 환자에 있어서 결핵의 발생이 문제가 되고 있으며, 특히 다제내성균주의 출현은 큰 문제이다. 결핵진단법 중 체액에서 도말검사나 배양검사를 통해서 Myco-bacterium tuberculosis를 검출하는 것이 표준진단법이나, 도말검사는 상대적으로 민감도가 낮고 배양검사는 오랜시간이 소요된다. 중함연쇄반응을 이용하여 결핵균의 DNA를 검출하는 방법은 매우 민감하나 비용이 많이 소요된다. ELISA(enzyme linked immunosorbent assay)를 이용하여 혈청에서 결핵균 특이항원에 대한 항체를 검출하는 방법이 객담도말음성인 활동성 폐결핵환자를 다른 비결핵성 폐질환으로부터 감별하는데 유용한지를 확인하고자 하였다. 방 법: 연세대학교 세브란스 병원에 입원한 183명의 객담도 말음성인 폐질환환자에서 항산균에 대한 객담도말 및 배양검사와 결핵균 특이 항원인 recombinant 38 kilo-dalton 항원과 culture filtrate항원을 이용한 ELISA 검사를 시행하였다. 결 과: 35명의 활동성 폐결핵환자 중 18명에서 배양검사상 Mycobacterium tuberculosis를 검출할 수 있었다. 38 kDa와 culture filtrate에 대한 흡광도는 활동성 폐결핵환자들에서 비결핵성 폐질환자들보다 유의하게 높았다 (p<0.05). 활동성 폐결핵환자 중 객담배양양성과 음성군사이에 38 kDa와 culture filtrate에 대한 홉광도의 유의한 차이는 없었다 (p>0.05) 객담도말음성인 환자에 있어서 38 kDa과 culture filtrate의 민감도는 각각 20.0%와 31.4%였고 특이도는 각각 95.3%와 93.9%였다. 결 론: 객답도말검사 음성인 활동성 폐결핵환자에 있어서 ELISA를 이용한 38 kDa과 culture filtrate 항원에 대한 혈청항체검사법은 기존진단방법보다 낮은 민감도를 보여, 비결핵성 폐질환으로부터 활동성 폐결핵을 감별진단하는데 크게 도움이 되지 않을 것으로 여겨진다.

• 가족자원경영과 정책
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• 제9권3호
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• pp.69-95
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• 2005

The purpose of this study was to analyze the needs and demands of middle-aged women concerning the educational contents of tea-culture, the managing methods of educational programs, and a plan to invigorate tea culture education as a whole. Here is the summary of the main results. 1) The need for tea-culture education scored an average of 4.14 / 5(maximum score). The actual needs for tea-culture education had varied according to the women's place of living, educational motives, and the desire to become lecturers in the future. That is, the actual needs for tea-culture education scored high among the groups who displayed strong personal motives to cultivate their inner minds and learn tea-culture seriously, groups who lived in Seoul${\cdot}$Taegu${\cdot}$Cheonju, and groups who wanted to become future lecturers. 2) The need for a systematic management of tea-culture educational programs scored an average of 4.10 / 5(maximum score). The need for a management of tea-culture educational programs varied according to the women's educational level and their desire to become future lecturers. That is, the need for a systematic management of tea-culture educational programs scored high among groups whose educational level was high, and groups who wanted to become future lecturers. 3) The need for a plan to invigorate tea-culture education scored an average of 4.05 / 5(maximum score). The need for a plan to invigorate tea-culture education varied according to the women's place of living, educational level, the managing subject of educational organizations, the desire to become future lecturers. That is, the need for a plan to invigorate tea-culture education scored high among the groups who lived in Seoul${\cdot}$Taegu${\cdot}$Cheonju, groups whose educational level was high, groups who were members of life-long educational facilities within universities, and groups who desired to become future lecturers.

• 복식
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• 제55권2호
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• pp.143-152
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• 2005

Stepping in the 1990s, we has lived under the time of co-existence of diverse culture as well as faced the diffusion of pop-culture. The border between subculture style and hi-fashion has been blurred since beginning of 1990s. By this reason, recognizing the power of influence and importance of subculture style, we will try to provide the comprehensive vision reflecting the cultural background to analyze the subculture style. We will conclude this article with discussing the club-culture which is settle as one type of culture. Enjoying the club-culture, clubber tries to escape from the structural estrangement putting by the magical and symbolic meaning through creating their own style, and endowing their life the meaning. They make liquid club wear targeting at general young culture, pop culture, and subculture as well as hippies, Mods, funk. The Club wear around University sort out Hiphop Style, Casual Style, Mix and Match style. As at the club the hiphop music was played, the hiphop style dress becomes the club ware. Especially, training style clothes which is easy to dance has become the everyday dress, not just restricted in the indoor dress.

• Journal of Animal Science and Technology
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• 제59권11호
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• pp.24.1-24.14
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• 2017

Over the past decades, in vitro culture media have been developed to successfully support IVF embryo growth in a variety of species. Advanced reproductive technologies, such as somatic cell nuclear transfer (SCNT), challenge us with a new type of embryo, with special nutritional requirements and altered physiology under in vitro conditions. Numerous studies have successfully reconstructed cloned embryos of domestic animals for biomedical research and livestock production. However, studies evaluating suitable culture conditions for SCNT embryos in wildlife species are scarce (for both intra- and interspecies SCNT). Most of the existing studies derive from previous IVF work done in conventional domestic species. Extrapolation to non-domestic species presents significant challenges since we lack information on reproductive processes and embryo development in most wildlife species. Given the challenges in adapting culture media and conditions from IVF to SCNT embryos, developmental competence of SCNT embryos remains low. This review summarizes research efforts to tailor culture media to SCNT embryos and explore the different outcomes in diverse species. It will also consider how these culture media protocols have been extrapolated to wildlife species, most particularly using SCNT as a cutting-edge technical resource to assist in the preservation of endangered species.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2007년도 추계 종합학술대회 논문집
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• pp.672-676
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• 2007

최근 들어 문화 콘텐츠의 급속한 발전이 있었음에도 불구하고 문화 콘텐츠 전문양성과 관련한 연구는 거의 없다. 따라서 본 연구의 목적은 문화 콘텐츠와 관련된 국내외 사례를 기반으로 최근 이슈가 되고 있는 문제점을 파악하고자 하였다. 본 연구 목적을 달성하기 위해 해외 사례로서 미국, 일본, 영국, 호주, 캐나다 등 5개국의 문화 콘텐츠 전문 양성 기관에 대한 현황에 대해 살펴보았으며, 국내 대학 기관의 교육 현황에 대해 살펴보았다. 이를 기반으로 국내 문화 콘텐츠 전문 인력 양성에 있어 문제점을 파악하고, 이를 해결하기 위한 제안을 제시하였다.

• Journal of Sport and Applied Science
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• 제4권3호
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• pp.1-10
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• 2020

Purpose: This study is to explore which genre of sports culture contents will show the most consistent growth in the future and analyse the development outlook based on those results. Research design, data, and methodology: Case study was used for this research as it seemed to be the most appropriate approach in order to obtain the most complete and meaningful information from a particular phenomenon. A total of 15 participants were chosen to participate in a semi-structured in-depth interview and the recordings were typed out as text. In order to ensure validity and credibility of findings, peer and member check were conducted. Results: As a result, the sports culture content that is most likely to consistently grow in the future is games. Additionally, it is expected that sports culture contents will develop as new categories of sports culture contents emerge, as the evolution of sports culture content occurs through the combination with technology, as well as the activation of sensual sports contents, and the production of sports culture contents utilizing big data. Conclusions: It is concluded that convergence of sport, cultural contents, and technology will promptly progress, and it will promote the development of sport culture contents and related industry.

• 한국직업건강간호학회지
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• 제21권3호
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• pp.266-273
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• 2012

Purpose: The objective of this study was to investigate the relationship among self-efficacy, nursing organizational culture and the emotional labor in clinical nurses. Methods: The subjects of this study were the clinical nurses working at 3 general hospitals in D city. The self-reported questionnaires were administered to 293 clinical nurses and data were analyzed with SPSS/WIN 19.0. Results: The levels of self-efficacy, nursing organizational culture and emotional labor of the subjects were revealed moderate to high. Significant explanatory variables of the degrees of self-efficacy included age, career of work and job satisfaction and nursing organizational culture included position, nursing method and job satisfaction and emotional labor included education, number of patient and job satisfaction. Correlations between self-efficacy and emotional labor and nursing organizational culture and emotional labor were negative while self-efficacy and nursing organizational culture were positive. Conclusion: The findings suggested the intervention program in order to improve job performance and job satisfaction, develop effective nursing organizational culture and reduce role conflicts among clinical nurses.