• Horticultural Science & Technology
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• v.28 no.3
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• pp.449-455
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• 2010

'Moulinrouge' was selected as the best regenerating cultivar among 18 different spray-type chrysanthemum cultivars bred in the Gyeongnam Flowers Breeding Research Institute. When the leaf explants from standard- and spray-type chrysanthemum 'Jinba' and 'Moulinrouge' were incubated on MS basal medium supplemented with $0.5mg{\cdot}L^{-1}$ BA and $1.0mg{\cdot}L^{-1}$ NAA, both 'Jinba' and 'Moulinrouge' induced adventitious shoots that can be regenerated into plantlets. Based on these regeneration conditions, we developed an efficient $Agrobacterium$-mediated chrysanthemum 'Moulinrouge' transformation method by using sequential selection of shoots from low ($10mg{\cdot}L^{-1}$) to high ($30mg{\cdot}L^{-1}$) concentrations of kanamycin after co-cultivation of leaf explants with $Agrobacterium$ for 10 days and induction of shoots. All kanamycin resistant plants investigated with genomic PCR analysis carried the report gene, $AtSICKLE$, in their genome. Although expression levels of the report gene in the transgenic plants investigated with RT-PCR were relatively low because of inefficiency of CaMV 35S promoter in chrysanthemum, transgenic lines expressing $AtSICKLE$ efficiently showed leaf epinasty phenotype. We expect that our results will provide a useful method that can perform a high-throughput investigation of genes isolated and studied well in model plants for molecular breeding of chrysanthemum.

• Journal of Plant Biotechnology
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• v.33 no.1
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• pp.57-62
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• 2006

Several methods for determining the response of corn to glyphosate were investigated to provide a fast and reliable method for identifying glyphosate-resistant corn in vivo. Two bioassays were developed. One assay is named 'whole plant / leaf growth assay', in which the herbicide glyphosate is applied on the upper part of 3rd leaf and the growth of herbicide-untreated 4th leaf is measured at 3 day after treatment. in this assay, the leaf growth of conventional corn was inhibited in a dose dependent from 50 to $1600{\mu}g/mL$ of glyphosate and growth inhibition at $1600{\mu}g/mL$ was 55% of untreated control. The assay has the potential to be used especially in the case that the primary cause of glyphosate resistance is related with a reduction of the herbicide translocation. Another assay is named 'leaf segment / shikimate accumulation assay', in which the four excised leaf segments ($4{\times}4mm$) are placed in each well of a 48-well microtiter plate containing $200{\mu}L$ test solution and the amount of shikimate is determined after incubation for 24 h in continuous light at $25^{\circ}C$. In this assay, 0.33% sucrose added to basic test solution enhanced a shikimate accumulation by 3 to 4 times and the shikimate accumulation was linearly occurred from 2 to $8{\mu}g/mL$ of glyphosate, showing an improved response to the method described by Shaner et al. (2005). The leaf segment / shikimate accumulation assay is simple and robust and has the potential to be used as a high throughput assay in the case that the primary cause of glyphosate resistance is related with EPSPS, target site of the herbicide. Taken together, these two assays would be highly useful to initially select the lines obtained after transformation, to investigate the migration of glyphosate-resistant gene into other weeds and to detect a weedy glyphosate-resistant corn in field.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.6
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• pp.519-522
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• 2004

In the course of anaerobic storage of excess sludge, odors due to chemicals such as hydrogen sulfide are produced. These odors cause many problems. Many methods have been developed to eliminate odors, but all current methods are not only costly, but also largely inef­fective. In this paper, we investigate the process of transformation of sludge microorganism cul­tures through intense aeration under nutrient-poor conditions, in terms of the selective adjust­ment and control of microorganism culture. The aerated sludge is subsequently returned to the adjusting pool, where the microorganisms inhibit odors, thus the excess sludge itself will act as an odor inhibitor. The process can be verified in terms of viability, in that the degradation capac­ity of the sludge was maintained after the intensely-aerated sludge was returned to the treat­ment system.

• Journal of applied mathematics & informatics
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• v.29 no.5_6
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• pp.1557-1569
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• 2011

In this paper, we consider the oscillation of the following certain second order nonlinear differential equations $(r(t)(x^{\prime}(t))^{\alpha})^{\prime}+q(t)x^{\beta}(t)=0$>, where ${\alpha}$ and ${\beta}$ are ratios of positive odd integers. New oscillation theorems are established, which are based on a class of new functions ${\Phi}={\Phi}(t,s,l)$ defined in the sequel. Also, we establish some interval oscillation criteria for this equation.

• Journal of Surface Science and Engineering
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• v.29 no.6
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• pp.884-890
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• 1996

We have investigated a structural change of Si(100)-($2 \times 1$)-Sb surface caused by atomic hydrogen adsorption at room temperature using time-of-flight impact collision ion scattering spectroscopy (TOF-ICISS) and low energy electron diffraction (LEED). We found that when atomic hydrogen adsorbs on the Si(100)-($2 \times 1$)-Sb surface, (1) the partial desorption of Sb atoms from the Si(100) surface occurs even at room temperature, (2) the rest Sb atoms are displaced from their original positions and form an almost two-dimensional layer with dispersive distribution of Sb atoms, and (3) the structural transformation into the Si(100)-($1 \times 1$)-H periodicity is induced by the formation of the $1 \times 1$-H dihydride phase on the Si substrate.

• Proceedings of the IEEK Conference
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• 2000.07b
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• pp.877-880
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• 2000

In this paper, we develop an algorithm of soccer image sequences mosaicing using reverse affine transform. The continuous mosaic images of soccer ground field allows the user/viewer to view a “wide picture” of the player’s actions The first step of our algorithm is to automatic detection and tracking player, ball and some lines such as center circle, sideline, penalty line and so on. For this purpose, we use the ground field extraction algorithm using color information and player and line detection algorithm using four P-rules and two L-rules. The second step is Affine transform to map the points from image to model coordinate using predefined and pre-detected four points. General Affine transformation has many holes in target image. In order to delete these holes, we use reverse Affine transform. We tested our method in real image sequence and the experimental results are given.

• Journal of Korean Society on Water Environment
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• v.23 no.5
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• pp.723-730
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• 2007

This study was conducted to develop a combined method for remediating a Chlorinated Aliphatic Hydrocarbons (CAHs) mixtures-contaminated aquifer. The process is consist of two processes. A chemical process (1st) using natural zinc ores for reducing higher concentrations of CAH mixtures to the level at which biological process is feasible; and A biological process (2nd) using aerobic cometabolism for treating lower concentration of CAH mixtures (less than 1 mg/L). Natural zinc ore showed relatively high transformation capacity, average dehalogenation percentage, and the best economic efficiency in previously our study. To evaluate the feasibility of the process, we operated two columns in series (that is, chemical and biological columns). In the first column filled with natural zinc ore and sand, CAH mixtures were effectively transformed with more than 95% efficiency, the efficiency depends on the Empty Bed Contact Time (EBCT) and the mass of zinc ore packed. Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD) analysis were performed to make sure whether natural zinc ore played an key role in the dechlorination of the CAH mixtures. The characteristics of zinc metal surface changed after exposure to CAHs due to oxidation of $Zn^0$ to $Zn^{2+}$. In the biological column injecting propane, DO and effluent of the chemical column, only 1,1,1-TCA was cometabolically transformed. Consequently, the combined process would be effective to remediate an aquifer contaminated with high concentrations of CAH mixtures.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.82-88
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• 2017

Transgenic lily plants have been obtained after particle bombardment, using PDS-1000/He system and scale explants of lilies, followed by PPT (D-L-phosphinothricin) selection. In this study, scales of the lily plants cv. 'red flame' were bombarded with a plasmid containing the bar gene as a selectable marker, and the AtSIZ gene as a gene of interest, showing salt tolerance and drought tolerance respectively, and both being driven by the CaMV 35S promoter. For optimization of a protocol, factors which optimized and showed a high transformation efficiency under following conditions, were considered: a bombardment pressure of 1100 psi, a target distance of 6 cm and $1.0{\mu}m$ of gold particle, and 24-h pre-culture and post-culture on MS medium containing 0.2 M sorbitol and 0.2 M mannitol as osmoticum agents. After bombardment, all the bombarded scales of lily were transferred to MS medium without selective agents, for a week. Subsequently, these bombarded scales were transferred to a selection MS medium containing 10 mg/l PPT, and incubated for a month for further selection, after which they were cultured for another 4-8 weeks with a 4-week subculture regime on the same selection medium. After transferring into hormone-free MS medium, the PPT-resistant scales with shoots were successfully rooted and regenerated into plantlets. PCR analysis revealed that the surviving putatively transformed plantlets indicated the presence of both the bar gene and the AtSIZ gene. In conclusion, when 100 scales of lily cv. Red flame are bombarded, this study produced approximately 17-18 transgenic plantlets with an optimized bombardment protocol. The protocol described here can contribute to the breeding program of lilies.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.36 no.10
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• pp.1221-1226
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• 2012

The hot-stamping technique is a forming method used for manufacturing high-strength parts, in which a part is cooled rapidly after press forming above the austenite transformation temperature. Boron steel, which contains a very small amount of boron, is one of the materials used for hot stamping. The purpose of this study is to investigate the mechanical properties of boron steel according to the heat-treatment conditions and the formability by using an Erichsen cupping test. Die quenching from various temperatures was conducted for different elapsed heat-treatment times. Laser-welded boron steel after quenching at 1173 K-0 s has a tensile strength of 1203 MPa. This is 79% of the tensile strength of the base metal (1522 MPa). The formability of boron steel was not significantly different from that at the mold temperature. However, it decreased with increasing forming speed. These properties provide practical information for the use of boron steels for hot stamping.

• Applied Biological Chemistry
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• v.39 no.4
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• pp.260-264
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• 1996

Process of particle bombardment for efficient transformation of Cymbidium virescence rhizome microcross sections was investigated using Biolistic particle delivery system with pBI121 harboring the ${\beta}-glucuronidase$(GUS) and the neomycin phosphotransferaseII(nptII). The best result was obtained from the combination of $1.11{\;}{\mu}m$ tungsten particles coated with pBl121, $77.33kg/cm^2$ helium pressure, 6.35 mm gap distance, and 7.0 cm target distance. Transient expression of the reporter gene, GUS, bombarded into the rhizome microsections was observed by the histochemical assay. The marker gene, nptII, delivered by bombarding the tungsten particles coated with the plasmid DNA was identified in the transformed rhizome by polymerase chain reaction.