• Journal of Microbiology and Biotechnology
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• 제17권12호
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• pp.1955-1964
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• 2007

A recent report showed that analysis of CD154 expression in the presence of the secretion inhibitor Brefeldin A (Bref A) could be used to assess the entire repertoire of antigen-specific $CD4^+\;T$ helper cells. However, the capacity of intracellular CD154 expression to identify antigen-specific $CD8^+\;T$ cells has yet to be investigated. In this study, we compared the ability of intracellular CD154 expression to assess antigen-specific $CD8^+\;T$ cells with that of accepted standard assays, namely intracellular cytokine IFN-${\gamma}$ staining (ICS) and MHC class I tetramer staining. The detection of intracellular CD154 molecules in the presence of Bref A reflected the kinetic trend of antigen-specific $CD8^+\;T$ cell number, but unfortunately showed less sensitivity than ICS and tetramer staining. However, ICS levels peaked and saturated 8 h after antigenic stimulation in the presence of Bref A and then declined, whereas intracellular CD154 expression peaked by 8 h and maintained the saturated level up to 24 h post-stimulation. Moreover, intracellular CD154 expression in antigen-specific $CD8^+\;T$ cells developed in the absence of $CD4^+\;T$ cells changed little, whereas the number of IFN-${\gamma}$-producing $CD8^+\;T$ cells decreased abruptly. These results suggest that intracellular CD154 could aid the assessment of antigen-specific $CD8^+\;T$ cells, but does not have as much ability to identify heterogeneous $CD4^+\;T$ helper cells. Therefore, the combined analytical techniques of ICS and tetramer staining together with intracellular CD154 assays may be able to provide useful information on the accurate phenotype and functionality of antigen-specific $CD8^+\;T$ cells.

• 대한간호학회지
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• 제50권3호
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• pp.487-500
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• 2020

Purpose: This study aimed to evaluate the validity and reliability of the Korean version of Menopause-Specific Quality of Life (MENQOL). Methods: The MENQOL was translated into Korean according to algorithm of linguistic validation process. A total of 308 menopausal women were recruited and assessed using the Korean version of MENQOL (MENQOL-K), the World Health Organization Quality of Life Brief Version (WHOQOL-BREF), and Center for Epidemiological Studies Depression Scale (CES-D-K). In estimating reliability, internal consistency reliability coefficients were calculated. Validity was evaluated through criterion validity and construct validity with confirmatory factor analyses using SPSS 23.0 and AMOS 25.0 software. Results: In item analyses, the "increased facial hair" symptom was excluded because of the low contribution of MENQOL-K. The confirmatory factor analysis supported good fit and reliable scores for MENQOL-K model, and the four-factor structure was validated (χ²=553.28, p<.001, NC=1.84, RMSEA=.05, AGIF=.85, AIC=765.28). The MENQOL-K consists of 28 items in 4 domains, including vasomotor (3 items), psychosocial (7 items), physical (15 items), and sexual subscales (3 items). There was an acceptable criterion validity with moderately significant correlation between MENQOL-K and WHOQOL-BREF. The Cronbach's α for the 4 subsacles ranged from .80 to .93. Conclusion: The MENQOL-K is a valid and reliable scale to measure condition-specific quality of life for perimenopausal and postmenopausal women. It can be used to assess the impact of menopausal symptoms on the quality of life of Korean women in clinical trials.

• 한국대기환경학회지
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• 제33권6호
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• pp.533-543
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• 2017

As the public interest in environmental issues increased, the "Act On The Integrated Control Of Pollutant-Discharging Facility" was enacted. Through the integrated environmental pollution prevention act in which 19 industries with large environmental impacts are sequentially applied, pollutants can be managed in a medium-integrated manner and integrated permission of the business unit is possible and BAT can be applied to enable a scientific and proactive environmental management system. In order to facilitate the implementation of integrated environmental pollution prevention act with these advantages, the BAT BREF should be published, modified and revised every 5 years considering the level of scientific development. This study reviewed the issues to be considered in applying BAT and the types of BAT and focused on presenting improvements and development direction when revising and supplementing the standards in the future based on these contents. For this purpose, when revising the standards, it will be necessary to reflect on the domestic situation, to expand the TWG(Technical Working Group) of small-scale workplace experts, and to exchange opinions with business places that have similar processes for each waste type through a systematic total inspection. In addition to these methods, by establishing a resident participation system through information disclosure, it is expected to be used as a guideline for environmental management of business places not subject to integrated permission of less than 3 types as well as those subject to integrated permission.

• 대한한의학회지
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• 제41권2호
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• pp.96-106
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• 2020

Objectives: The purpose of this study was to research the demographical and gynecologic characteristics, quality of life, and analyze the pattern identification in female patients with cold hypersensitivity of hands and feet. Methods: 112 participants were recruited from May 2018 to April 2019 from three korean medical hospitals. The data was composed of general characteristics, degree of coldness of hands and feet, gynecological questionnaire, score of WHOQOL-BREF and pattern identification. We divided data into 2 groups: The group of patients who meet the criteria for diagnosis of coldness on feet and the group of patients who meet the criteria for diagnosis of coldness on feet and hands. We tried to compare data by groups using Excel 2016 for windows and SPSS for windows (Ver. 23.0). Results: Throughout the study, we found differences between 2 groups in weight, body mass index (BMI), VAS score of cold hypersensitivity on feet, temperature of PC8, ST32, and pattern identification. Conclusions: After the study, we confirmed that even though both groups suffer from cold hypersensitivity of hands and feet, they show clinical differences in each group and differences in pattern identification.

• 한국사회복지학
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• 제57권1호
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• pp.219-243
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• 2005

본 연구에서는 한국판 세계보건기구 삶의 질 척도 단축형(WHOQOL-BREF)을 사용하여 우리 사회의 대표적 빈곤층인 노숙자들이 자신의 삶의 질을 어떻게 평가하며, 또 이들의 삶의 질에 어떤 요인들이 영향을 미치는가를 살펴보았다. 연구의 대상은 20세 이상 된 185명의 노숙자이며, 조사기간은 2003년 9월 18일부터 27일까지 10일간이었다. 연구결과, 노숙자의 삶의 질이 낮다는 것이 실증적으로 확인되었다. 또한 삶의 질 하위영역별로 예측요인이 다소 다르긴 하지만, 이들의 삶의 질에 영향을 미치는 요인으로 연령, 공공부조 수급, 인지된 건강상태, 음주, 알코올중독, 거주형태, 타인의 인정이 중요하게 나타났다. 이러한 결과를 바탕으로, 노숙자의 삶의 질 향상을 위하여 주거최우선정책이 필요함을 강조하였고, 알코올중독 문제와 노숙자에 대한 사회적 편견 해소를 위한 대책방안에 대해서도 논의하였다.

• 한국전문물리치료학회지
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• 제24권1호
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• pp.51-60
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• 2017

Background: In cerebral palsy (CP), parents' quality of life and rearing attitude are considered possible factors that influence patients' quality of life, function level, and performance in daily activities. Despite these facts, little attention has been given to demonstrate a relation between these factors. Objects: This study aimed to demonstrate the relationship between parents' quality of life, functional level, and performance in daily activities, the quality of life of school-aged children with spastic CP. Methods: This study included 24 parents of school-aged children with spastic CP. The KIDSCREEN-52 and World Health Organization quality of life (WHOQOL)-BREF questionnaires (including physical, psychological, social, and environmental domains) were used as research tools to assess the quality-of-life profiles of the children with spastic CP and those of their parents, respectively. In addition, the function levels and performance in daily activities of the children with CP were assessed by using the gross motor function measure (GMFM) and modified Barthel index (MBI). Correlation and multiple regression analyses were performed to clarify the determinants of the quality of life of the children with CP. Results: The KIDSCREEN-52 score correlated with the total score (r=.735, p<.01) and all domains of the WHOQOL-BREF questionnaire (physical: r=.542, p<.01; psychological: r=.690, p<.01; social: r=.568, p<.01; and environmental: r=.783, p<.01). In addition, significant correlation was found between the KIDSCREEN-52 and MBI scores (r=.411, p<.05), and between the MBI and GMFM scores (r=.427, p<.05). After controlling for age, gender, paralytic type, GMFM, and MBI, the WHOQOL-BREF score ($R^2=.621$), particularly in the environmental domain ($R^2=.699$), remained independently related to the quality of life of the children. Conclusion: These findings suggest that the quality of life of school-aged children with spastic CP can be influenced by the quality of life of their parents. This study provides useful information for future studies to investigate the quality of life of children with CP.

• Journal of Acupuncture Research
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• 제28권1호
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• pp.117-124
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• 2011

Objectives : Bell's palsy is the most common acute facial paralysis but its causes still unclear. At present, one of the most widely accepted cause is viral infections, and generally socioeconomic factors influence the viral infections. The purpose of this study is to investigate the relationship of incidence of facial palsy with socioeconomic factors. Methods : Seventeen patients with a acute facial palsy, who volunteered and completed all of the assessment measures participated. Using data on 17 participants, we examined the independent contribution of education, income, and occupation to a risk factor of severe facial palsy. Severity of global facial impairment was assessed by the facial disability index (FDI), the house-brackmann facial nerve grading system, WHO quality of life - bref (WHOQOL-BREF) and visual analogue scale (VAS) about discomfort of life. Results : There was no correlation between severity of facial palsy and gender, marriage, education, or occupation. Age greater than 60 years (p<0.05), and low monthly income(p<0.05), poor self-rated health was associated with greater severity of idiopathic facial paralysis. Conclusions : The number of subjects with facial palsy in our study (n-17) was small, and therefore generalization to larger patient populations might be unwarranted. But according to the outcome, we suggest that socioeconomic factors, especially low monthly income influence severity of bell's palsy.

• Journal of Microbiology and Biotechnology
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• 제21권9호
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• pp.937-946
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• 2011

Several isolates of Bacillus thuringiensis (Bt) were screened for the vegetative insecticidal protein (Vip) effective against sap-sucking insect pests. Screening results were based on $LC_{50}$ values against cotton aphid (Aphis gossypii), one of the dangerous pests of various crop plants including cotton. Among the isolates, the Bt#BREF24 showed promising results, and upon purification the aphidicidal protein was recognized as a binary toxin. One of the components of this binary toxin was identified by peptide sequencing to be a homolog of Vip2A that has been reported previously in other Bacillus spp. Vip2 belongs to the binary toxin group Vip1-Vip2, and is responsible for the enzymatic activity; and Vip1 is the translocation and receptor binding protein. The two genes encoding the corresponding proteins of the binary toxin, designated as vip2Ae and vip1Ae, were cloned from the Bt#BREF24, sequenced, and heterologously expressed in Escherichia coli. Aphid feeding assay with the recombinant proteins confirmed that these proteins are indeed the two components of the binary toxins, and the presence of both partners is essential for the activity. Aphid specificity of the binary toxin was further verified by ligand blotting experiment, which identified an ~50 kDa receptor in the brush border membrane vesicles of the cotton aphids only, but not in the lepidopteran insects. Our finding holds a promise of its use in future as a candidate gene for developing transgenic crop plants tolerant against sap-sucking insect pests.

• 재활복지공학회논문지
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• 제9권2호
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• pp.89-103
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• 2015

본 연구는 척수손상 환자를 대상으로 병원기반 사회복귀 지원프로그램을 개발하고, 프로그램 시행 전 후를 비교 분석함으로써 프로그램의 효과성을 평가하고자 하였다. 재활병원에 입원해 있는 25명의 척수손상 환자를 대상으로 2~3개월 동안 일주일에 2번 평균 6.1개의 프로그램을 실시하였다. 프로그램 시행 전, 후 COPM, HADS, AD-R, WHOQOL-BREF를 통해 작업 수행도 및 만족도, 불안과 우울, 장애수용, 삶의 질을 비교하였으며, 퇴원 시 CIQ로 지역사회통합 수준과 퇴원 후의 사회복귀여부를 측정하였다. 자료분석은 기술통계와 대응표본 t검정(paired t-test)을 실시하였다. 연구결과 병원기반 사회복귀 지원프로그램 실시 후 작업 수행도 및 만족도, 장애수용, 삶의 질에 향상을 보였다(p<0.05). 또한 퇴원 후 25명의 척수손상 환자 중에서 10명이 가정, 학업, 직업으로 복귀하였다. 이를 통해 병원기반 사회복귀 지원프로그램이 척수손상 환자들이 퇴원 후 그들이 속한 지역사회로 돌아가는데 효과적임을 알 수 있었다. 척수손상 환자들이 가정과 사회로의 빠른 복귀를 위해서는 사회복귀를 위한 다양한 프로그램 개발과 더불어 체계적인 사회복귀 연계시스템이 이루어져야 할 것으로 사료된다.

• The Journal of Korean Physical Therapy
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• 제30권1호
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• pp.35-40
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• 2018

Purpose: This study examined whether the quality of life of parents of children with cerebral palsy is affected by the functions, activities, participation, and environmental factors using an ICF-CY check list. Methods: This study recruited in 26 parents of children with cerebral palsy. The functions, activities, participation, and environmental factors of the children were evaluated using ICF-CY checklist. To measure the quality of life of parents, this study used world health organization quality of life (WHOQOL-BREF), which was composed of a total of 26 questions. The GMFCS (gross motor function classification system) was used to assess the degree of disability in the children. Multiple regression analysis was performed to examine the effects of the ICF-CY checklist on the quality of life. Correlation analysis was performed to examine the correlation between GMFCS and WHOQOL. Results: The functions, activities, participation, and environmental factors were significantly different from WHOQOL-BREF. On the other hand, the contextual factor showed a significant difference in the neuromusculoskeletal and movement-related functions (b7), and service, systems, and policies (e5)(p<0.05). Conclusion: This study suggests that the functions and environmental factors affect the quality of life of parents of children with cerebral palsy. Therefore, these findings suggest that contextual factors, such as neuromusculoskeletal and movement-related functions (b7), and service, systems and policies (e5), which can be facilitators, should be considered for improving the quality of life of parents of children with cerebral palsy.