• Development and Reproduction
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• v.11 no.2
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• pp.121-125
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• 2007

Morphological changes on digestive tract were observed using Flounder, Paralichthys olivaceus to elucidate the early developmental processes. Hatched larvae, ($2.50{\pm}0.14\;mm$ in total length: TL) has not opened its mouth and anus. The digestive tract showed linear tubular form from mouth to anus extending along with yolk. On $7{\sim}8$ days from hatching, the post-larvae, ($5.05{\pm}0.40\;mm$ TL) has developed digestive tract and activated for feeding. The digestive tract expanded into dorsal side, joined to intestine, and the stomach began to develop. The digestive tract was divided into an anterior and posterior region, and began to differentiate. In the post-larvae($6.50{\pm}0.25\;mm$ TL) on $13{\sim}14$ days from hatching, the digestive tract twisted one cycle in the anterior region, the area was expanded. The digestive tract was completely differentiated into oesophagus, anterior intestine, middle intestine, posterior intestine and arranges fundamental structure. In the embryo($12.40{\pm}0.55\;mm$ TL) after 30 days from hatching, stomach became large and could be distinguished from cardia and pyloric parts apparently. The digestive tract developed similar with that of adult flounder showing clear division of stomach, anterior intestine, middle intestine, posterior intestine and rectum. In the embryo($16.15{\pm}0.85\;mm$ TL) after 40 days from hatching, esophagus, pyloric part, anterior intestine, middle intestine, and rectum have largely expanded, and thus the morphological features are somewhot different with those of post-larvae flounder.

• IMMUNE NETWORK
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• v.3 no.2
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• pp.126-135
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• 2003

Background: One of the important factors in the prognosis of chronic hepatitis B patient is the degree of replication of hepatitis B virus (HBV). It has been known that HBV DNA polymerase plays the essential role in the replication of HBV. HBV DNA polymerase is composed of four domains, TP (Terminal protein), spacer, RT (Reverse transcriptase) and RNaseH. Among these domains, tyrosine, the $65^{th}$ residue of TP is an important residue in protein-priming reaction that initiates reverse transcription. If monoclonal antibody that recognizes around tyrosine residue were selected, it could be applied to further study of HBV replication. Methods: To produce TP-specific scFv (single-chain Fv) by phage display, mice were immunized using synthetic TP-peptide contains $57{\sim}80^{th}$ amino acid residues of TP domain. After isolation of mRNA of heavy-variable region ($V_H$) and light-chain variable region ($V_L$) from the spleen of the immunized mouse, DNA of $V_H$ and $V_L$ were obtained by RT-PCR and joined by a DNA linker encoding peptide (Gly4Ser)3 as a scFv DNA fragments. ScFv DNA fragments were cloned into a phagemid vector. scFv was expressed in E.coli TG1 as a fusion protein with E tag and phage gIII. To select the scFv that has specific affinity to TP-peptide from the phage-antibody library, we used two cycles of panning and colony lift assay. Results: The TP-peptide-specific scFv was isolated by selection process using TP-peptide as an antigen. Selected scFv had 30 kDa of protein size and its nucleotide sequences were analyzed. Indirect- and competitive-ELISA revealed that the selected scFv specifically recognized both TP-peptide and the HBV DNA polymerase. Conclusion: The scFv that recognizes the TP domain of the HBV DNA polymerase was isolated by phage display.

• Proceedings of the Korea Contents Association Conference
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• 2009.05a
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• pp.420-424
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• 2009

In this research, to identify the degree of preference and participation in leisure sports, over 19-year-old adults living in the capital region were collected as subjects, and among them, 250 persons were chosen by sampling. Using SPSSWIN 16.0, I analyzed the collected data by Reliability Analysis, Paired Sample T-Test, and IP A method. The conclusion is the following. First, the first quadrant showed skins-cuba, water-ski, wind surfing, and clay-pigeon shooting, and as a result of this, it is necessary to improve the degree of participation in this field. Secondly, the second quadrant showed golf, snow board, ski, fishing, and climbing, and these field need to specific attention to maintain continuous attention, for they have a great deal of attendance. Thirdly, in the third quadrant, there are water sleigh, cart, balloon, sky-diving, orienteering, hang gliding, model plane, and survival game. As the degree of preference and participation in these sports is low, it is recommended that more effort should be made for these sports. Finally, in the forth quadrant, there are tracking, mountain bike, inline skates, rafting, and cycling. As it's the participation is high, while that of preference is low, it is important to maintain the policy of the participation in leisure sports. In conclusion, for the sports which is preferred that is difficult to join right now, most of all, it is necessary that we should pay attention to and invest the social infrastructure in which main items cannot be joined now.

• Strategy21
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• s.38
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• pp.287-320
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• 2015

On October 27, 2015, USS Lassen(DDG82), a 9,200 ton class Aegis destroyer of the United States Navy, began its operations within 12 nautical miles of Subi Reef, one of the seven artificial islands that China has built and claimed sovereignty over. The maneuver was joined by anti-submarine patrol airplanes such as P-8A and P-3. The White House press secretary mentioned that the President of the United States approved the operation. In response, China announced that it warned the US Navy ship about the 'illegal violation' by sending two destroyers(PLAN Lanzhou and Taizhou). This event represents a close call case where tension between the United States and China in the South China Sea might have been elevated to a conflict between the two navies. Moreover, considering that this happened only one month after Chinese president Xi's state visit to the United States, the event shows that the positions of the two countries have become starkly different to the extent that they are so hard to be reconciled. The United States' position is different from those of Vietnam and the Philippines. Countries like Vietnam and the Philippines have been directly involved in disputes with regard to sovereignty claims across the waters in the South China sea. As for the United States, being a third party in the disputes, it still cannot be a by-stander watching the whole waters in the region fall under the influence of China. Accordingly, the United States maintains that all countries bear the rights of innocent passage and military operations in the Exclusive Economic Zones(EEZ) as stipulated by the United Nations Convention on the Law of the Sea(UNCLOS). In contrast, China claims that, historically, the South China sea has been part of China's territorial waters, and that foreign countries are not allowed to conduct military operations within the waters. It strongly accuses that such military operations are illegal. Against this background, this paper tracks the different positions of the United States and China on the issues regarding the South China sea. It also carefully looks at the possibility that, in the process of dealing with the issues, the two countries may get into an armed conflict as the phrase 'Thucydides Trap' predicts.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.38 no.8
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• pp.857-862
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• 2014

The research on a thermal stress control layer (TSCL) begins to undertake to reduce residual stress and strain in the vicinity of the joined region between the hardfacing layer and the base part. The goal of this paper is to estimate the material combination and the thickness of TSCL for the Stellite21 hardfaced STD61 hot working tool steel via three-dimensional finite element analysis (FEA). TSCL is created by the combination of Stellite21 and STD61. The thickness of TSCL ranges from 0.5 mm to 1.5 mm. The influence of the material combination and the thickness of TSCL on temperature, thermal stress and thermal strain distributions of the hardfaced part have been investigated. The results of the investigation have been revealed that a proper material combination of TSCL is Stellite21 of 50 % and STD61 of 50 %, and its appropriate thickness is 1.0 mm.

• Journal of Environmental Impact Assessment
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• v.14 no.5
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• pp.347-353
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• 2005

The risk assessment is the qualitative or quantitative evaluation of the risk posed to human health and the environment by the actual or potential presence or release of hazardous substances, pollutants or contaminants. The environmental impact assessment (EIA) is assessed by the environmental criteria, and risk assessment is assessed by the risk rate. Risk rate based on dose-response values may not be easy to apply on regulatory basis like EIA for uncertainty. Internationally there is an example of OECD program. Risk assessment of High Production Volume (HPV) Chemicals has started since the OECD Program with the 1990 Council Act on the Co-operative Investigation and Risk Reduction of Existing Chemicals. These HPV chemicals include all chemicals produced or imported at levels greater than 1,000 tonnes per year in at least one Member country or in the European Union region. The SIDS called the Screening Information Data Set is regarded as the minimum information needed to assess an HPV chemical to determine whether any further work should be carried out or not. All the data elements of SIDS including assessment for environment and health are prepared as three formats of the full SIDS Dossier, the SIDS Initial Assessment Report (SIAR), and the SIDS Initial Assessment Profile (SIAP) of an HPV chemical. In 1998 the global chemical industry through the International Council of Chemical Associations (ICCA) has joined to work with OECD. The OECD has assessed approximately 1,000 chemicals from 1991 through 2004 with ICCA. Till the February of 2005, 592 chemicals of those chemicals completed SIDS reports. Member countries have been targeted the goal of 1,000 new chemicals from 2005 to 2010 and Korea shared 36 chemicals from the 1,000 new chemicals. Currently Korea has completed SIDS reports of 7 chemicals among sponsored 24 chemicals. In conclusion SIDS project will be linked to national program for outputs application with more reliable production. Both the OECD and industry will carry out their commitment to complete assessments for more and the remaining chemicals assessment. The major outputs will contribute to cope with international chemical management.

• Journal of the Korean Geographical Society
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• v.51 no.2
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• pp.235-253
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• 2016

China has been developing a new auto industry growth plan since 2004. In line with this initiative, China actively adopted its policy favoring foreign companies' investment which had a competitive edge over their technologies and manufacturing methodologies. To meet this demand in policy and market change, many foreign auto companies and their parts' manufacturers including Korean auto companies joined this stream. Policy change favoring higher technologies applicable in China requested auto companies' swift adaptation to meet the policy requirements by higher technologies with innovation and introduction of those foreign technologies to China. The spatial (excellence) strategy was followed by the increase in its efficiency and competiveness of each region, which were materialized by or in the form of; Firstly, strategic partnership with China auto companies and encouragement of Korea auto parts manufacturing companies to set up its own factories in China. Secondly, modularization and platform sharing strategy by applying enhanced technologies. Thirdly, strategic utilization of China local government's incentive policies. As production management methodology, JIS was adopted all across the board to meet the on-demand market requirements in the manufacturing processes. Auto part manufacturers had been integrated regionally based on forward linkages and modules. As a result, regional-specific auto industry complexes have been made in the places such as Beijing-Hyundai in the north, Dongfeng-Yueda-Kia in the south, common auto parts at central area like Qingdao, and other parts and raw materials in the vicinity of Shanghai.

• Journal of Korean Society of Forest Science
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• v.101 no.1
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• pp.36-45
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• 2012

Education on the environment and plants offered by arboretum for today's people not only contribute to foster a better natural environment in urban region but also provide visitors with decent refreshment environment and beyond. In the study, the author undertook the observation on usage behavior and satisfaction model of arboretum visitors expect and investigated the facilities and programs to be offered by arboretum in order to propose the opinion regarding the service. For observation size of variables in a multiple regression analysis of variables is influencing satisfaction rankings walks the line of flow, the educational effect on the environment, cleanliness of the facility, visits pay, natural beauty, diversity of trees, accessibility and friendliness of staff, expansion of facilities in the arboretum and appeared as a complement. In case of visitor attribute, the residents living near the facility showed the highest visit frequency of more than 5 times, especially as part of taking a walk. This proves that the visit to arboretum is considered as part of everyday life, and thus a new program and walk path as well as movement route are needed to be developed for the visitors. In the question relating to the facilities and operation programs in Daegu Arboretum, particularly the requests by visitors, they responded that the establishment of cultural event, beautiful natural scenery, refreshment and convenience facilities is the most critical issue. In addition, the management on withered trees and bare lands is an urgent issue as well. In this sense, the Operation and Management Strategies based upon the visitor behaviors and model of satisfaction are needed to deal with the adoption of diverse events and festivals joined by local residents, ombudsman program, environmental program development for students and teachers within the region, negligent bare lands and withered tree replacement, and cafeteria facility improvement and supplement as well as the bench marking of other facilities than arboretums located in other regions. These items are thought to be sufficiently dealt with by Daegu Arboretum having no more external resources. It is recognized that the visitor satisfaction begins from a minor thing, and a small difference determines a great satisfaction, and thus the software approach rather than hardware one is in need.

• Journal of Korean society of Dental Hygiene
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• v.1 no.2
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• pp.227-242
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• 2001

The purpose of this study was 10 examine whether or not the dental- health education experiences of elementary school teachers have any influence on their oral-health awareness and attitude. The subjects in this study were 414 selected teachers from Seoul and North Cholla province. The instrument used by Lee Heung-su was modified into self- reporting questionnaire, and the schools where the subjects were working were visited to conduct a survey for 28 days from September 15 to October 13, 2000. For data analysis, SPSS was employed, and chi-squre, t-test and ANOVA were implemented. The findings of this study could be listed as below: 1. 184(44.4%) out of the techers investigated had ever been educated in dental health, and 230 teachers(55.6%) hadn't. 47% replied they hadn't received oral-health education for the lack of education opportunities. 2. In regard to dental-health awareness and attitude. the presence or absence of dental-health education experiences didn't make any difference to their daily toothbrushing frequency(p>0.05). The use of fluorine was significantly different between the groups(p<0.01), as 42.9% of the teachers with dental-health education experiences and 63.9% of the others with no such a experience didn't use that, 45.7% of the former group and 29.1 % of the latter accurately knew how to brush teeth, and the difference between the two was significant (p<0.01). 3. Concerning student-related dental-health awareness and attitude, the dental-health education experiences make 45.7% of the educated group and 31.7% of the uneducated group agreed to the strong need for oral examination, and the gap between the two was significant(p<0.01). 90.8% of the former group gave counsel to students on dental health, and 77.2% of the latter didn't. The gap between the two was significant as well (p<0.01). 77.2% of the educated group and 41.3% of the uneducated group offered frequent dental-health education, and the difference between the two was significant (p<0.01). 4. Their awareness of the fluorine-based toothbrushing project differed significantly by region(p<0.05), as the teachers from North Cholla province recognized it better than those from Seoul. The female teachers provided more education regarding that project, and those who were older or had more teaching experiences were more aggressive in instructing that project(p<0.01). And the married teachers took more forward attitude than the unmarried (p<0.01). By school location, the teachers from Seoul showed more active attitude than those from North Cholla province(p<0.01), and the gap between the two was significant. As to cooperation, those who were older or had more teaching experience were more cooperative, and the married teachers joined forces better than the unmarried. The gap was significant (p<0.01). 5. Concerning the awareness and attitude of the fluorine-based toothbrushing project, the educated teachers took more aggressive attitude, and the difference between the two was significant(p<0.01). The above-mentioned findings suggested that the dental-health awareness and attitude of the elementary school teachers were under a lot of influence of their oral-health education experiences, and there is a need to develop and carry out education programs for teacher.

• Journal of Genetic Medicine
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• v.5 no.1
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• pp.15-20
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• 2008

Purpose : Large exon deletions in the DMD gene are found in about 60% of DMD/BMD patients. Multiplex PCR has been employed to detect the deletion mutation, which frequently generates noise PCR products due to the presence of multiple primers in a single reaction as well as the stringency of PCR conditions. This often leads to a false-negative or false-positive result. To address this problematic issue, we introduced the dual primer oligonucleotide (DPO) system. DPO contains two separate priming regions joined by a polydeoxyinosine linker that results in high PCR specificity even under suboptimal PCR conditions. Methods : We tested 50 healthy male controls, 50 patients with deletion mutation as deletion-positive patient controls, and 20 patients with no deletions as deletion-negative patient controls using DPO-multiplex PCR. Both the presence and extent of deletion were verified by simplex PCR spanning the promoter region (PM) and 18 exons including exons 3, 4, 6, 8, 12, 13, 17, 19, 43-48, 50-52, and 60 in all 120 controls. Results : DPO-multiplex PCR showed 100% sensitivity and specificity for the detection a deletion. However, it showed 97.1% sensitivity and 100% specificity for determining the extent of deletions. Conclusion : The DPO-multiplex PCR method is a useful molecular test to detect large deletions of DMD for the diagnosis of patients with DMD/BMD because it is easy to perform, fast, and cost-effective and has excellent sensitivity and specificity.