• Polymer(Korea)
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• v.38 no.5
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• pp.613-619
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• 2014

The polypropylene/polyamide elastomer (PP/PAE) blends were prepared by melt mixing. PP and PAE in PP/ PAE were immiscible completely. The size of PAE domains was large and the clear gap in the interface between PP and PAE existed, which did not meet the conditions enhancing toughness of polymers by elastomer. Therefore, maleic anhydride grafted polypropylene (MP) was used to improve the miscibility between PP and PAE. The miscibility between PP and PAE was improved and the size of dispersed phase PAE decreased by introducing MP. The crystallization of PP became easier by introducing PAE as a nucleating agent. With the increase of PAE content, the melt-crystallization temperatures of PP components in PP/PAE/MP blends increased gradually. The melt-crystallization of the polytetramethylene oxide segment of PAE component in PP/PAE blends were hampered by PP component. In addition, PAE can enhance significantly the toughness of PP, and the tensile strength and modulus did not decrease.

• Korean Journal of Mathematics
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• v.17 no.4
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• pp.473-480
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• 2009

We prove the existence and multiplicity of nontrivial solutions for a fourth order problem ${\Delta}^2u+c{\Delta}u={\alpha}u-{\beta}(u+1)^-$ in ${\Omega}$, ${\Delta}u=0$ and $u=0$ on ${\partial}{\Omega}$, where ${\lambda}_1{\leq}c{\leq}{\lambda}_2$ (where $({\lambda}_i)_{i{\geq}1}$ is the sequence of the eigenvalues of $-{\Delta}$ in$H_0^1({\Omega})$) and ${\Omega}$ is a bounded open set in $R^N$ with smooth boundary ${\partial}{\Omega}$. The results are proved by applying minimax arguments and linking theory.

• Journal of Information Processing Systems
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• v.17 no.2
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• pp.334-336
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• 2021

• Food Science of Animal Resources
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• v.40 no.4
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• pp.527-540
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• 2020

Donkey in China is well known for its draft purpose and transportation; however, donkey meat has attracted more and more consumers in recent years, yet it lacks sufficient information on its flavor components compared to other main meats. Therefore, in this study, volatile flavor compounds in neck meat of donkey, swine, bovine, and sheep were classified by electronic nose, then confirmed and quantified by gas chromatography-mass spectrometry. High-performance liquid chromatography (HPLC) and gas chromatography were used to quantify free fatty acid, amino acid, and flavor nucleotide. A total of 73 volatile compounds were identified, and aldehydes were identified as the characteristic flavor compounds in neck meat of donkey, bovine, swine and sheep in proportion of 76.39%, 46.62%, 31.64%, and 35.83%, respectively. Particularly, hexanal was the most abundant volatile flavor. Compared with other neck meat, much higher unsaturated free fatty acids were present in donkeys. Furthermore, neck meat of donkeys showed essential amino acid with highest content. Thus, special flavor and nutrition in donkey neck meat make it probably a candidate for consumers in other regions besides Asia.

• Journal of Microbiology and Biotechnology
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• v.24 no.2
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• pp.168-176
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• 2014

The morphology of filamentous fungi closely correlates with the productivity in submerged culture. Using itaconic acid (IA) production by Aspergillus terreus as a research model, the quantitative relationship between the growth form of A. terreus and IA production was investigated. IA fermentation was scaled up from shake flasks to a 7 L stirred tank bioreactor based on the quantitative relationship. Our results demonstrated the following: (1) Three morphologies of A. terreus were formed by changing the inoculum level and shape of the flask. (2) Investigation of the effects of the three morphologies on broth rheology and IA production revealed the higher yield of IA on dry cell weight (DCW, IA/DCW) and yield of glucose on DCW (consumed glucose/DCW) were achieved during clump growth of A. terreus. (3) By varying the $KH_2PO_4$ concentration and culture temperature, the relationships between clump diameter and IA production were established, demonstrating that the yield of IA on DCW ($R^2$ = 0.9809) and yield of glucose on DCW ($R^2$ = 0.9421) were closely correlated with clump diameter. The optimum clump diameter range for higher IA production was 0.40-0.50 mm. (4) When the clump diameter was controlled at 0.45 mm by manipulating the mechanical stress in a 7 L fermentor, the yield of IA on DCW and yield of glucose on DCW were increased by 25.1% and 16.3%, respectively. The results presented in this study provide a potential approach for further enhancement of metabolite production by filamentous fungi.

• Journal of Microbiology and Biotechnology
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• v.30 no.2
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• pp.196-205
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• 2020

In this study, the acidic lipase from Aspergillus niger (ANL) was homologously expressed in A. niger. The expression of ANL was significantly improved by the expression of the native ANL with the introns, the addition of the Kozak sequence and the optimization of the signal sequences. When the cDNA sequence of ANL fused with the glaA signal was expressed under the gpdA promoter in A. niger, no lipase activity could be detected. We then tried to improve the expression by using the full-length ANL gene containing three introns, and the lipase activity in the supernatant reached 75.80 U/ml, probably as a result of a more stable mRNA structure. The expression was further improved to 100.60 U/ml by introducing a Kozak sequence around the start codon due to a higher translation efficiency. Finally, the effects of three signal sequences including the cbhI signal, the ANL signal and the glaA signal on the lipase expression were evaluated. The transformant with the cbhI signal showed the highest lipase activity (314.67 U/ml), which was 1.90-fold and 3.13-fold higher than those with the ANL signal and the glaA signal, respectively. The acidic lipase was characterized and its highest activity was detected at pH 3.0 and a temperature of 45℃. These results provided promising strategies for the production of the acidic lipase from A. niger.

• Journal of Microbiology and Biotechnology
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• v.24 no.10
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• pp.1368-1376
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• 2014

This article focuses on the effects of glycine betaine on preventing caramelization, and increasing DCW and $\small{L}$-lysine production. The additional glycine betaine not only decreased the browning intensity (decreased 4 times), and the concentrations of 5-hydroxymethylfurfural (decreased 7.8 times) and furfural (decreased 12 times), but also increased the availability of glucose (increased 17.5%) for $\small{L}$-lysine production. The DCW and $\small{L}$-lysine production were increased by adding no more than 20 mM glycine betaine, whereas the DCW and $\small{L}$-lysine production were decreased with the reduction of pH values, although pH had a better response to prevent caramelization than did glycine betaine. For $\small{L}$-lysine production, the highest increase (40%) was observed on the media with 20 mM glycine betaine. The crucial enzymes in glycolysis and $\small{L}$-lysine biosynthesis pathway were investigated. The results indicated that additional glycine betaine increases the activity of enzymes in glycolysis, in contrast to the effect of pH. All the results indicated that glycine betaine can be used to prevent caramelization and increase the $\small{L}$-lysine production. By applying this strategy, glucose would not be have to be separated from the culture media during autoclaving so that factories can save production costs and shorten the fermentation period.

• Journal of Microbiology and Biotechnology
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• v.19 no.1
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• pp.65-71
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• 2009

Microbial oxidoreductive systems have been widely used in asymmetric syntheses of optically active alcohols. However, when reused in multi-batch reaction, the catalytic efficiency and sustainability of non-growing cells usually decreased because of continuous consumption of required cofactors during the reaction process. A novel method for NADPH regeneration in cells was proposed by using pentose metabolism in microorganisms. Addition of D-xylose, L-arabinose, or D-ribose to the reaction significantly improved the conversion efficiency of deracemization of racemic 1-phenyl-1,2-ethanediol to (S)-isomer by Candida parapsilosis cells already used once, which afforded the product with high optical purity over 97%e.e. in high yield over 85% under an increased substrate concentration of 15 g/l. Compared with reactions without xylose, xylose added to multi-batch reactions had no influence on the activity of the enzyme catalyzing the key step in deracemization, but performed a promoting effect on the recovery of the metabolic activity of the non-growing cells with its consumption in each batch. The detection of activities of xylose reductase and xylitol dehydrogenase from cell-free extract of C. parapsilosis made xylose metabolism feasible in cells, and the depression of the pentose phosphate pathway inhibitor to this reaction further indicated that xylose facilitated the NADPH-required deracemization through the pentose phosphate pathway in C. parapsilosis. moreover, by investigating the cofactor pool, the xylose addition in reaction batches giving more NADPH, compared with those without xylose, suggested that the higher catalytic efficiency and sustainability of C. parapsilosis non-growing cells had resulted from xylose metabolism recycling NADPH for the deracemization.

• Computers and Concrete
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• v.30 no.5
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• pp.339-355
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• 2022

The high cost of ultra-high-performance engineered cementitious composite (UHP-ECC) is currently a crucial issue, especially in terms of the polyethylene (PE) fibers use. In this paper, cheap calcium carbonate whiskers (CW) were evaluated on the feasibility of hybrid with PE fibers. Diverse combinations of PE fibers and CW were employed to investigate the multi-scale enhancement on the UHP-ECC performance. A probabilistic-based UHP-ECC tensile strain reliability analysis approach was utilized, which was in general agreement with the experimental results. Furthermore, a multi-dimensional integrated representation was conducted for the comprehensive assessment of UHP-ECC. Results illustrated that CW improved the compressive strength and energy dissipation capacity of UHP-ECC owing to the microscopic strengthening mechanism. CW and PE fiber further promoted the saturated cracking of composite by multi-scale crack arresting effect. In particular, PE1.75-CW0.5 specimen possessed the best overall performance. The ultimate cracking width of PE1.75-CW0.5 group had 98 ㎛, which was 46.18% lower compared to PE2-CW0 group, the 28d compressive strength were slightly improved, the tensile strain capacity was comparable to that of PE2-CW0 group. The results above demonstrated that combinations of PE fiber and CW could significantly enhance the comprehensive performance of UHP-ECC, which was beneficial for large-scale engineering applications.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.34 no.4
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• pp.98-109
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• 2016

The purpose of this study is to identify characteristics of spatial structure of Jiangnan water town in China which has been developed along the water channel. Spatial structural characteristics of the water town, Wuzhen and Nanxun in Zhejiang Province are as follows. First, location of town, spatial framework, lot division, and architectural style get determined by the water channel. The water channel is constructed in cross shape, T-shape, or in combination. Secondly, public space of water town is established along main water channel. Visually and functionally alternating private buildings are established on the water channel side and they are creating unique landscape of Jiangnan water town in overall harmony. Thirdly, residential area is established on the east-west water channel side with southward sunny-faced arrangement and waterside residence is in introvert spatial structure with courtyard but the exterior space is connected with water channel intimately. Fourthly, various spatial components along the water channel enrich the sense of place in water town. The components are corridor with roof, tea house, waterside steps, waterside theater and watergate etc. Uniqueness of place is becoming the core competence in modern townscape. In this aspect, Jiangnan water town with historical and cultural accumulation is suggesting a significant implication. Major implications are as follows. First, promotion of proximity to the water is the basic value that the spatial plan of waterfront needs to pursue. Secondly, mixed use is essential for effective land use and revitalization of waterfront. Thirdly, waterfront plan based on the local uniqueness as the place asset is being required.