• Journal of Korean Neurosurgical Society
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• v.58 no.2
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• pp.101-106
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• 2015

Objective : The aim of this study was to explore the immunity in rats transplanted with adipose-derived mesenchymal stem cells (ADSCs) and acellular nerve (ACN) for repairing sciatic nerve defects. Methods : ADSCs were isolated from the adipose tissues of Wistar rats. Sprague-Dawley rats were used to establish a sciatic nerve defect model and then divided into four groups, according to the following methods : Group A, allogenic nerve graft; Group B, allograft with ACN; Group C, allograft ADSCs+ACN, and Group D, nerve autograft. Results : At the day before transplantation and 3, 7, 14, and 28 days after transplantation, orbital venous blood of the Sprague-Dawley rats in each group was collected to detect the proportion of $CD3^+$, $CD4^+$, and $CD8^+$ subsets using flow cytometry and to determine the serum concentration of interleukin-2 (IL-2), tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) and $interferon-{\gamma}$ ($IFN-{\gamma}$) using enzyme-linked immunosorbent assay (ELISA). At each postoperative time point, the proportion of $CD3^+$, $CD4^+$, and $CD8^+$ subsets and the serum concentration of IL-2, $TNF-{\alpha}$, and $IFN-{\gamma}$ in group C were all near to those in group B and group D, in which no statistically significant difference was observed. As compared with group A, the proportion of $CD3^+$, $CD4^+$, and $CD8^+$ subsets and the serum concentration of IL-2, $TNF-{\alpha}$, and $IFN-{\gamma}$ were significantly reduced in group C (p<0.05). Conclusion : The artificial nerve established with ADSCs and ACN has no obvious allograft rejection for repairing rat nerve defects.

• Computers and Concrete
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• v.10 no.2
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• pp.135-147
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• 2012

At mesoscale, concrete is considered as a three-phase composite material consisting of the aggregate particles, the cement matrix and the interfacial transition zone (ITZ). The reconstruction of the internal structures for concrete composites requires the identification of the boundary of the aggregate particles and the cement matrix using digital imaging technology followed by post-processing through MATLAB. A parameter study covers the subsection transformation, median filter, and open and close operation of the digital image sample to obtain the optimal parameter for performing the image processing technology. The subsection transformation is performed using a grey histogram of the digital image samples with a threshold value of [120, 210] followed by median filtering with a $16{\times}16$ square module based on the dimensions of the aggregate particles and their internal impurity. We then select a "disk" tectonic structure with a specific radius, which performs open and close operations on the images. The edges of the aggregate particles (similar to the original digital images) are obtained using the canny edge detection method. The finite element model at mesoscale can be established using the proposed image processing technology. The location of the crack determined through the numerical method is identical to the experimental result, and the load-displacement curve determined through the numerical method is in close agreement with the experimental results. Comparisons of the numerical and experimental results show that the proposed image processing technology is highly effective in reconstructing the internal structures of concrete composites.

• Journal of the Economic Geographical Society of Korea
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• v.21 no.2
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• pp.107-118
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• 2018

Due to the rapid economic development, traffic congestion has become a dire concern in Beijing, China. Levying a congestion toll is seen as the most effective solution to the problem. Building a congestion pricing model is a crucial step in implementing a workable toll scheme. Unlike previous attempts, this study not only covers the theoretical discussion but also considers three practical issues: the speed-density relationship, the value of travel time savings, and the determination of optimal traffic volume. We estimate the speed-density relationship by regression models and the value of travel time saved through survey results. We further suggest a way through which the government could identify the optimal traffic flow by a series of trial-and-errors, without the knowledge of exact road demand structure. Finally, a practical tolling scheme is proposed for Beijing's second ring road along with some policy recommendations.

• The Journal of the Korea institute of electronic communication sciences
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• v.9 no.12
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• pp.1345-1352
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• 2014

Bus architecture of SoC has been replaced by NoC in recent years. Noc uses the multi-clock domains to transmit and receive data between neighbor network interfaces and they have same frequency, but a phase difference because of clock skew. So a synchronizer is used for a mesochronous frequency in interconnection between network interfaces. In this paper the metastability is defined and analyzed in a D latch and a D flip-flop to search the possibilities that data can be lost in the process of sending and receiving data between interconnects when a local frequency and a transmitted frequency have a phase difference. 180nm CMOS model parameter and 1GHz are used to simulate them in HSpice. The simulation results show that the metastability happens in a latch and a flip-flop when input data change near the clock edges and there are intermediate states for a longer time as input data change closer at the clock edge. And the next stage can lose input data depending on environmental conditions such as temperature, processing variations, power supply, etc. The simulation results are very useful to design a mescochronous synchronizer for NoC.

• BMB Reports
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• v.38 no.5
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• pp.571-576
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• 2005

Cytokines are proteins produced by many different cells of the immune system and play a significant role in initiating and regulating the inflammatory process. In this research, an important cytokine, interleukin-10 (IL-10) gene, has been identified and characterized from zebrafish (Danio rerio) genome database. Zebrafish IL-10 is located within a 2690 bp fragment and contains five exons and four introns, sharing the same organization with mammalian IL-10 genes. An open reading frame of 543 bp was found to encode a putative 180 amino acid protein with a signal peptide of 22 amino acids, which shares 29.7-80.9% homology with amino acid sequences of other known IL-10. The signature motif of IL-10 is also conserved in zebrafish IL-10. The predicted transcript was finally confirmed by sequencing of cDNA clones. Multi-tissue reverse transcriptase PCR (RT-PCR) was performed to examine the tissue distribution and expression regulation of this gene in seven organs of normal and lipopolysaccharide (LPS) stimulation zebrafish. The results demonstrated that this gene was expressed slightly in normal kidney, gill and gut, no expression was detected in other four tissues. The expression was clearly upregulated after LPS stimulation. Using the ideal zebrafish model, further study of IL-10 characterization and function may provide insight on the understanding of the innate immune system.

• Structural Engineering and Mechanics
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• v.50 no.2
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• pp.241-255
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• 2014

The girder of self-anchored suspension bridge is subjected to large compression force applied by main cables. So, serious damage of the girder due to breakage of hangers may cause collapse of the whole bridge. With the time increasing, the hangers may break suddenly for their resistance capacities decrease due to corrosion. Using nonlinear static and dynamic analysis methods and adopting 3D finite element model, the responses of a concrete self-anchored suspension bridge to sudden breakage of hangers are studied in this paper. The results show that the sudden breakage of a hanger has significant effects on tensions of the hangers next to the broken hanger, bending and torsion moments of the girder, moments of the towers and reaction forces of the bearings. The results obtained from dynamic analysis method are very different from those obtained from static analysis method. The maximum tension of hanger produced by breakage of a hanger exceeds 2.2 times of its initial value, the maximum dynamic amplification factor reaches 2.54, which is larger than the value of 2.0 recommended for cable-stayed bridge in PTI codes. If two adjacent hangers on the same side of bridge break one after another, the maximum tension of other hangers exceeds 3.0 times of its initial value. If the safety factor adopted to design hanger is too small, or the hangers have been exposed to corrosion, the bridge may collapse due to breakage of two adjacent hangers.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.12
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• pp.6463-6468
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• 2012

Purpose: To evaluated the effect of the gambogic acid (GA), one of the effective components of Garcinia, in combination with a new multi-targeted oral medication, sunitinib (SU) on renal cancer cell proliferation in vitro and on tumor growth in vivo. Methods: After treatment with GA or SU, either alone or in combination, MTT and FACS analysis were used to examine cell viability and cycle distribution of the renal carcinoma cell lines 786-0 and Caki-1. Western blotting was employed to examine the expression of proteins related to the cell cycle and vascular formation. Furthermore, a xenograft model was applied to study the antitumor efficacy of SU or GA alone or in combination, with immunohistochemistry to detect expression of proteins related to xenograft growth and angiogenesis. Western blotting was used to examine NF-${\kappa}B$ signaling pathway elements in xenografts. Results: Treatment of 786-0 and Caki-1 cells with GA or SU resulted in decreased tumor cell proliferation, especially with joint use. Cells accumulated more strongly in the sub-G1 phase after joint treatment with GA and SU than treatment of GA and SU alone. Western blotting arrays showed 1 protein significantly upregulated, 2 proteins downregulated, and 2 proteins unchanged. Moreover, combined use of GA and SU inhibited the growth and angiogenesis of xenografts generated from Caki-1 significantly. Immunohistochemistry arrays showed downregulation of the expression of proteins promoting xenograft growth and angiogenesis, and Western blotting showed inhibition of the NF-${\kappa}B$ signaling pathway after treatment by GA alone and in combination with SU in xenografts. Conclusions: Our results show that the joint use of GA and SU can provide greater antitumor efficacy compared to either drug alone and thus may offer a new treatment strategy for renal cell carcinoma.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.18
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• pp.7583-7588
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• 2014

Background: Colorectal cancer (CRC) is a major cause of cancer-related death and cancer-related incidence worldwide. The potential of microRNA-21 (miR-21) as a biomarker for CRC detection has been studied in several studies. However, the results were inconsistent. Therefore, we conducted the present meta-analysis to systematically assess the diagnostic value of miR-21 for CRC. Materials and Methods: Using a random-effect model, the pooled sensitivity (SEN), specificity (SPE), positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) were calculated to evaluate the diagnostic performance of miR-21 for CRC. A summary receiver operating characteristic (SROC) curve and an area under the curve (AUC) were also generated to assess the diagnosis accuracy of miR-21 for CRC. Q test and I2 statistics were used to assess between-study heterogeneity. Publication bias was evaluated by the Deeks' funnel plot asymmetry test. Results: A total of 986 CRC patients and 702 matched healthy controls from 8 studies were involved in the meta-analysis. The pooled results for SEN, SPE, PLR, NLR, DOR, and AUC were 57% (95%CI: 39%-74%), 87% (95%CI: 78%-93%), 4.4 (95%CI: 2.4-8.0), 0.49 (95%CI: 0.32-0.74), 9 (95%CI: 4-22), and 0.83 (95%CI: 0.79-0.86), respectively. Subgroup analyses further suggested that blood-based studies showed a better diagnostic accuracy compared with feces-based studies, indicating that blood may be a better matrix for miR-21 assay and CRC detection. Conclusions: Our findings suggest that miR-21 has a potential diagnostic value for CRC with a moderate level of overall diagnostic accuracy. Hence, it could be used as auxiliary means for the initial screening of CRC and avoid unnecessary colonoscopy, which is an invasive and expensive procedure.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.10 no.3
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• pp.1195-1211
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• 2016

The emerging video coding standard High Efficiency Video Coding (HEVC) has shown almost 40% bit-rate reduction over the state-of-the-art Advanced Video Coding (AVC) standard but at about 40% computational complexity overhead. The main reason for HEVC computational complexity is the inter prediction that accounts for 60%-70% of the whole encoding time. In this paper, we propose several fast coding unit (CU) encoding schemes based on the Merge mode and motion estimation information to reduce the computational complexity caused by the HEVC inter prediction. Firstly, an early Merge mode decision method based on motion estimation (EMD) is proposed for each CU size. Then, a Merge mode based early termination method (MET) is developed to determine the CU size at an early stage. To provide a better balance between computational complexity and coding efficiency, several fast CU encoding schemes are surveyed according to the rate-distortion-complexity characteristics of EMD and MET methods as a function of CU sizes. These fast CU encoding schemes can be seamlessly incorporated in the existing control structures of the HEVC encoder without limiting its potential parallelization and hardware acceleration. Experimental results demonstrate that the proposed schemes achieve 19%-46% computational complexity reduction over the HEVC test model reference software, HM 16.4, at a cost of 0.2%-2.4% bit-rate increases under the random access coding configuration. The respective values under the low-delay B coding configuration are 17%-43% and 0.1%-1.2%.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.7
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• pp.3309-3328
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• 2017

The application of Internet of Things (IoT) in the next generation cellular networks imposes a new characteristic on the data traffic, where a massive number of small packets need to be transmitted. In addition, some emerging IoT-based emergency services require a real-time data delivery within a few milliseconds, referring to as ultra-low latency transmission. However, current techniques cannot provide such a low latency in combination with a mice-flow traffic. In this paper, we propose a dynamic resource reservation schema based on an air-interface slicing scheme in the context of a massive number of sensors with emergency flows. The proposed schema can achieve an air-interface latency of a few milliseconds by means of allowing emergency flows to be transported through a dedicated radio connection with guaranteed network resources. In order to schedule the delay-sensitive flows immediately, dynamic resource updating, silence-probability based collision avoidance, and window-based re-transmission are introduced to combine with the frame-slotted Aloha protocol. To evaluate performance of the proposed schema, a probabilistic model is provided to derive the analytical results, which are compared with the numerical results from Monte-Carlo simulations.