• Korean Chemical Engineering Research
• /
• v.54 no.5
• /
• pp.678-686
• /
• 2016

The effect of internal and shroud nozzle distributor to bubbling fluidized beds which has the size of $0.3m-ID{\times}2.4m-high$ column was modeled by CPFD (Computational Particle-Fluid Dynamics). Metal-grade silicon particles (MG-Si) were used as bed materials which have $d_p=149{\mu}m$, ${\rho}_p=2,325kg/m^3$ and $U_{mf}=0.02m/s$. Total bed inventory and static bed height were 75 kg and 0.8 m, respectively. Effect of vertical internal on the bubble rising velocity was investigated. Bubbles were split by internal when the axial position of the internal from the distributor, z = 0.45 m. Bed pressure drop and axial solid holdup were not affected by internal. However, in the case that axial distance of internal from distributor was too close to jet penetration length, bubbles were not separated and bypassed internal, and faster than without internal or z = 0.45 m.

• Journal of Embryo Transfer
• /
• v.31 no.3
• /
• pp.161-168
• /
• 2016

Nucleotide metabolism in endothelium is variable between different species. Recent studies demonstrated that this variability could contribute coagulation dysfunction, even though organs of the alpha 1,3-galactosyltransferase gene knockout pig were transplanted into the primate. CD73 (ecto-5'-nucelotidase) is an enzyme at cell surface catalyzing the hydrolysis of adenosine triphosphate to adenosine, which plays role on a substance for anti-inflammatory and anti-coagulant. Thus, overexpression of CD73 in endothelial cells of the pig is considered as an approach to reduce coagulopathy. In this study, we constructed a human CD73 expression vector under control of porcine Icam2 promoter (pIcam2-hCD73), which is expressed specifically at endothelial cells, and of CMV promoter as a control (CMV-CD73). First, we transfected the CMV-CD73 vector into HEK293 cells, and then confirmed CD73 expression at cell surface by flow cytometry analysis. Next, we transfected the pIcma2-CD73 and CMV-CD73 vectors into primary porcine fibroblasts and endothelial cells. Consequence was that the pIcma2-CD73 vector was expressed only at the porcine endothelial cells, meaning that the pIcam2 promoter lead to endothelial cell-specific expression of CD73 in vitro. Finally, we nucleofected the pIcam2-hCD73 vector into passage 3 fibroblasts, and enforced hygromycin selection of 400mg/ml. We were able to obtain forty three colonies harboring pIcam2-CD73 to provide donor cells for transgenic cloned porcine production.

• Journal of Embryo Transfer
• /
• v.31 no.3
• /
• pp.169-177
• /
• 2016

There is a growing interest in the application of primary hepatocytes for treatment of liver diseases in humans and for drug development. Several studies have focused on long-term survival and di-differentiation blocking of primary hepatocytes in an in vitro culture system. Therefore, the present study also aimed to optimize an in vitro culture system using primary rat hepatocytes. Primary rat hepatocytes from 6-week-old male Crl:CD rats were isolated using a modified two-step collagenase perfusion. Healthy $3.5{\times}10^6$ primary rat hepatocytes were seeded into a 2 dimensional (2D) culture in a 25T culture flask coated with collagen type I or into a 3D culture in a 125-ml spinner flask for 7 days. Production of plasma protein (ALB and TF), apoptosis (BAX and BCL2), and CYP (CYP3A1) related genes were compared between the 2D and 3D culture systems. The 3D culture system had an advantage over the 2D system because of the relatively high expression of ALB and low expression of BAX in the 3D system. However, the level of CYP3A1 did not improve in the 3D culture with and without the presence of a dexamethasone inducer. Therefore, 3D culture has an advantage for albumin production and primary rat hepatocyte survivability, but a low expression of CYP3A1 indicated that primary rat hepatocytes require a high-density culture for stress reduction by continuous flow.

• Journal of Embryo Transfer
• /
• v.33 no.3
• /
• pp.185-194
• /
• 2018

The aim of this study was performed to evaluate the effects of ice-binding protein from the arctic yeast Leucosporidium (LeIBP) supplementation on cryopreservation of boar sperm. The collected semen was diluted ($1.5{\times}10^8/ml$) in lactose egg yolk (LEY) and cooled at $5^{\circ}C$ for 3 h. The cooled semen was then diluted ($1{\times}10^8/ml$) in LeIBP containing LEY with 9% glycerol and maintained at $5^{\circ}C$ for 30 min. The semen was divided into six experimental groups (control, 0.001, 0.005, 0.01, 0.05 and 0.1 mg/ml of LeIBP). The straws were kept on above the liquid nitrogen ($LN_2$) vapors for 20 minutes and then plunged into $LN_2$. After thawing, computer-assisted sperm analysis was used for sperm motility and flow cytometry was performed to assess the viability, acrosome integrity (FITC-PSA/PI), ROS (DCF/PI), lipid peroxidation (BODIPY C11/PI) and apoptosis (Annexin V/PI), respectively. No significant responses were observed for sperm motility. However, sperm viability was significantly increased on 0.05 and 0.1 mg/ml of LeIBP groups compared to control (P < 0.05). In addition, acrosome integrity was significantly increases LeIBP groups (P < 0.05) and both ROS and lipid peroxidation level were lower in all LeIBP groups than those of control (P < 0.05). On the other hand, a significant higher apoptosis rate was observed in 0.05 and 0.1 mg/ml of LeIBP groups compared to control (P < 0.05). It can be assumed that a supplementation of LeIBP in boar sperm freezing extender is an effective method to increase the sperm qualities after cryopreservation.

• Journal of Embryo Transfer
• /
• v.33 no.3
• /
• pp.129-137
• /
• 2018

Acute vascular rejection has been known as a main barrier occurring in a xenograted tissue of alpha 1,3-galactosyltransferase knock-out (GalT KO) pig into a non-human primate (NHP). Adenosine which is a final metabolite following sequential hydrolysis of nucleotide by ecto-nucleotidases such as CD39 and CD73, act as a regulator of coagulation, and inflammation. Thus xenotransplantation of CD39 and CD73 expressing pig under the GalT KO background could lead to enhanced survival of recipient NHP. We constructed a human CD39 and CD73 expression cassette designed for endothelial cell-specific expression using porcine Icam2 promoter (pIcam2-hCD39/hCD73). We performed isolation of endothelial cells (pAEC) from aorta of 4 week-old GalT KO and membrane cofactor protein expressing pig ($GalT^{-MCP/-MCP}$). We were able to verify that isolated cells were endothelial-like cells using immunofluorescence staining analysis with von Willebrand factor antibody, which is well known as an endothelial maker, and tubal formation assay. To find optimal condition for efficient transfection into pAEC, we performed transfection with GFP expression vector using four programs of nucleofection, M-003, U-023, W-023 and Y-022. We were able find that the program W-023 was optimal for pAEC with regard to viability and transfection efficiency by flow cytometry and fluorescent microscopy analyses. Finally, we were able to obtain $GalT^{-MCP/-MCP}/CD39/CD73$ pAEC expressing CD39 and CD73 at levels of 33.3% and 26.8%, respectively. We suggested that pACE isolated from $GalT^{-MCP/-MCP}$ pig might be provided as a basic resource to understand biochemical and molecular mechanisms of the rejections and as an alternative donor cells to generate $GalT^{-MCP/-MCP}/CD39/CD73$ pig expressing CD39 and CD73 at endothelial cells.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.48 no.2
• /
• pp.127-134
• /
• 2020

High supersonic aircraft are exposed to high temperature environments by aerodynamic heating during supersonic flight. Thermal protection system structures such as double-panel structures are used on the skin of the fuselage and wings to prevent the transfer of high heat into the interior of an aircraft. The thin-walled double-panel skin can be exposed to acoustic loads by supersonic aircraft's high power engine noise and jet flow noise, which can cause sonic fatigue damage. Therefore, it is necessary to examine the behavior of supersonic aircraft skin structure under thermal-acoustic load and to predict fatigue life. In this paper, we designed and fabricated thermal-acoustic test equipment to simulate thermal-acoustic load. Thermal-acoustic testing of the titanium specimen under thermal-acoustic load was performed. The analytical model was verified by comparing the thermal-acoustic test results with the finite element analysis results.

• Journal of Embryo Transfer
• /
• v.28 no.3
• /
• pp.193-198
• /
• 2013

This study was designed to evaluate the effect of L-cysteine on sperm characteristics and oocyte cleavage in vitro in Korean native cattle. For this study, the freezing of diluted semen were added with Triladyl containing 20% egg-yolk and/or 0, 5, 10 and 20 mM L-cysteine before cryopreservation. The viability in frozen-thawed sperm were estimated by SYBR14/PI double stain, acrosome damage with FITC-PNA, mitochondria intact with Rhodamin123 and hydrogen peroxide($H_2O_2$) level with carboxy-DCFDA by flow-cytometry. The developmental capacity was also assessed with cleavage rates in oocytes fertilized in vitro by frozen-thawed sperm. In results, the sperm viability was significantly increased in 10 mM and 20 mM concentrations of L-cysteine than other groups (p<0.05). In addition, acrosome damage was significantly decreased in 10 mM and 20 mM concentrations of L-cysteine than other groups (p<0.05). The mitochondria intact was also significantly increased in 10 mM and 20 mM concentrations of L-cysteine than other groups (p<0.05). On the other hand, the cleavage rates were significantly increased in 0 mM, 5 mM and 10 mM groups than 20 mM concentration of L-cysteine (p<0.05). The oocyte degeneration of oocytes were significantly decreased in 0 mM, 5 mM and 10 mM groups than in 20 mM L-cysteine group (P<0.05). However, there are no significantly differences among the L-cysteine treatment groups. We suggest that concentration of 10 mM L-cysteine have beneficial impact for sperm cryopreserved in Korean native cattle. This result also could be recommended for artificial insemination program if supported by an improvement in the fertility results and required further study.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.17 no.2
• /
• pp.141-151
• /
• 2015

A new rock excavation method using an abrasive injection waterjet system has been developed to enhance the efficiency and reduce the vibration of tunnel excavation. The abrasive feed rate is an important factor for the cutting performance and the economical efficiency of waterjet-based excavation. In this study, various experiments were performed to explore the effects of major process parameters for both the abrasive feed rate and the suction pressure occurring inside the mixing chamber when the abrasives are inhaled. Experimental results reveal that the abrasive feed rate is affected by geometry parameters (abrasive pipe height, length, and tortuosity), abrasive parameters (abrasive particle size), and jet energy parameters (water pressure and water flow rate). In addition, the relation between the cutting performance and the abrasive feed rate was discussed on the basis of the results of an experimental study. The cutting performance can be maximized when the abrasive feed rate is controlled appropriately via careful management of major process parameters.

• Journal of The Korean Astronomical Society
• /
• v.36 no.3
• /
• pp.81-87
• /
• 2003

It is suggested that a flying-by star in a hot accretion disk may cool the hot accretion disk by the Comptonization of the stellar emission. Such a stellar cooling can be observed in the radio frequency regime since synchrotron luminosity depends strongly on the electron temperature of the accretion flow. If a bright star orbiting around the supermassive black hole cools the hot disk, one should expect a quasi-periodic modulation in radio, or even possible an anti-correlation of luminosities in radio and X-rays. Recently, the unprecedentedly accurate infrared imaging of the Sagittarius A$\ast$ for about ten years enables us to resolve stars around it and thus determine orbital parameters of the currently closest star S2. We explore the possibility of using such kind of observation to distinguish two quite different physical models for the central engine of the Sagittarius A$\ast$, that is, a hot accretion disk model and a jet model. We have attempted to estimate the observables using the observed parameters of the star S2. The relative difference in the electron temperature is a few parts of a thousand at the epoch when the star S2 is near at the pericenter. The relative radio luminosity difference with and without the stellar cooling is also small of order $10^{-4}$, particularly even when the star S2 is near at the pericenter. On the basis of our findings we tentatively conclude that even the currently closest pass of the star S2 is insufficiently close enough to meaningfully constrain the nature of the Sagittarius A$\ast$ and distinguish two competing models. This implies that even though Bower et al. (2002)have found no periodic radio flux variations in their data set from 1981 to 1998, which is naturally expected from the presence of a hot disk, a hot disk model cannot be conclusively ruled out. This is simply because the energy bands they have studied are too high to observe the effect of the star S2 even if it indeed interacts with the hot disk. In other words, even if there is a hot accretion disk the star like S2 has imprints in the frequency range at v $\le$ 100 MHz.

• Proceedings of the Korean Society of Propulsion Engineers Conference
• /
• 2004.03a
• /
• pp.48-54
• /
• 2004

The effects of injector spacing s and injector diameter d on mixing are numerically investigated in supersonic combustor with perpendicular injection behind a backward-facing step. Simulations are reported for airstream Mach number of 2.4. Parameters are changed on following 4 cases to investigate the effects of injector configuration on mixing efficiency $\eta_m$. In the case of varying d or s, dynamic pressure ratio $Rq(=(pu^2)_j/(pu^2)_a)$ is also varied to keep bulk equivalence ratio $\Phi({\oe})Rq.d^2/s)$ constant. (l) Injector spacing s is varied at constant $\Phi$=0.5, 1, 2 for injector diameter d=6mm. In the case of $\Phi$=1, $\eta_m$ has its maximum value at s=24mm. The reason is that increase of $\eta_m$. , by widening spacing at Rq=constant competes with decrease of $\eta_m$ by increasing Rq at s=constant. When spacing is narrow, the flow field of vicinity of injector becomes two-dimensional because adjacent jets interferes each other. By widening spacing, air is easily entrained by three-dimensional effect. This mechanism also appears in the case of $\Phi$=0.5, 2 for d=6mm, and $\eta_m$. reaches its maximum value at s=24mm for $\Phi$=0.5 and at s=42mm for $\Phi$=2. (2) In the case of injector diameter d varied at $\Phi$=1 for s=30mm, $\eta_m$. has its maximum value at d=3mm. The reason is that decrease of $\eta_m$ by increasing injector diameter competes with increase of $\eta_m$ by decreasing Rq at d=constant.(3) In the case of s varied at $\Phi$=0.5, 1,2 for d=3mm, the injector spacing at which mixing efficiency has its maximum value is s= 18mm for $\Phi$=0.5, s=24mm for $\Phi$=1, s=24mm for $\Phi$=2. Therefore it is found that d=3mm and s=24mm can be optimum configuration over a range of $\Phi$=0.5~2.(4) The effect of h on the optimum spacing is investigated. s is varied for d=6mm at step height h=4, 6, 8mm. The simulation results do not show significant change on the step height.