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Analysis of the shelf life of chitosan stored in different types of packaging, using colorimetry and dentin microhardness

  • da Cruz-Filho, Antonio Miranda;de Vito, Angelo Rafael;Souza-Flamini, Luis Eduardo;da Costa Guedes, Debora Fernandes;Saquy, Paulo Cesar;Silva, Ricardo Gariba;Pecora, Jesus Djalma
    • Restorative Dentistry and Endodontics
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    • v.42 no.2
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    • pp.87-94
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    • 2017
  • Objectives: Chitosan has been widely investigated and used. However, the literature does not refer to the shelf life of this solution. This study evaluated, through the colorimetric titration technique and an analysis of dentin micro-hardness, the shelf life of 0.2% chitosan solution. Materials and Methods: Thirty human canines were sectioned, and specimens were obtained from the second and third slices, from cemento-enamel junction to the apex. A 0.2% chitosan solution was prepared and distributed in 3 identical glass bottles (v1, v2, and v3) and 3 plastic bottles (p1, p2, and p3). At 0, 7, 15, 30, 45, 60, 90, 120, 150, and 180 days, the specimens were immersed in each solution for 5 minutes (n = 3 each). The chelating effect of the solution was assessed by micro-hardness and colorimetric analysis of the dentin specimens. 17% EDTA and distilled water were used as controls. Data were analyzed statistically by two-way and Tukey-Kramer multiple comparison (${\alpha}=0.05$). Results: There was no statistically significant difference among the solutions with respect to the study time (p = 0.113) and micro-hardness/time interaction (p = 0.329). Chitosan solutions and EDTA reduced the micro-hardness in a similar manner and differed significantly from the control group (p < 0.001). Chitosan solutions chelated calcium ions throughout the entire experiment. Conclusions: Regardless of the storage form, chitosan demonstrates a chelating property for a minimum period of 6 months.

Removal of Organic Load from Olive Washing Water by an Aerated Submerged Biofilter and Profiling of the Bacterial Community Involved in the Process

  • Pozo, Clementina;Rodelas, Belen;Martinez-Toledo, M. Victoria;Vilchez, Ramiro;Gonzalez-Lopez, Jesus
    • Journal of Microbiology and Biotechnology
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    • v.17 no.5
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    • pp.784-791
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    • 2007
  • The present work aims to use a biofilter technology(aerated submerged filters) for the aerobic transformation at laboratory-scale of olive washing water(OWW) generated in the first steps of olive oil processing, as well as the genetic profiling and identification to the species level of the bacteria involved in the formation of the biofilm, by means of TGGE. Chemical parameters, such as biological oxygen demand at five days($BOD_5$) and chemical oxygen demand(COD), decreased markedly(up to 90 and 85%, respectively) by the biological treatment, and the efficiency of the process was significantly affected by aeration and inlet flow rates. The total polyphenol content of inlet OWW was only moderately reduced(around 50% decrease of the inlet content) after the biofilter treatment, under the conditions tested. Partial 16S rRNA genes were amplified using total DNA extracted from the biofilm and separated by TGGE. Sequences of isolated bands were mostly affiliated to the $\alpha-subclass$ of Proteobacteria, and often branched in the periphery of bacteria] genera commonly present in soil(Rhizobium, Reichenowia, Agrobacterium, and Sphingomonas). The data obtained by the experimentation at laboratory scale provided results that support the suitability of the submerged filter technology for the treatment of olive washing waters with the purpose of its reutilization.

Novel Sources of Resistance to Phytophthora capsici on Pepper (Capsicum sp.) Landraces from Mexico

  • Retes-Manjarrez, Jesus Enrique;Rubio-Aragon, Walter Arturo;Marques-Zequera, Isidro;Cruz-Lachica, Isabel;Garcia-Estrada, Raymundo Saul;Sy, Ousmane
    • The Plant Pathology Journal
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    • v.36 no.6
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    • pp.600-607
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    • 2020
  • Phytophthora capsici Leonian is a major pathogen of pepper worldwide and few resistance sources to this pathogen have been identified so far. The goals of this study were to identify new sources of resistance against P. capsici in Capsicum landraces and analyze the relationship between the resistance indicator of plant symptoms and some plant phenotype parameters of plant height, stem width, leaf length and leaf width. Thirty-two landraces of pepper were collected from fourteen states in Mexico. From each population, 36 plants were inoculated with 10,000 zoospores of P. capsici under controlled conditions. This experiment was repeated twice. Out of the 32 landraces, six showed high level of resistance, four showed intermediate resistance and five showed low level of resistance when compared with the susceptible control 'Bravo' and the resistant control 'CM334', indicating that these landraces are promising novel sources of resistance to P. capsici. There was no correlation between the symptoms and plant phenotype parameters. However, these parameters were not affected in the group classified as highly resistant, indicating that P. capsici does not affect the growing of these resistant pepper landraces. The other resistant groups were significantly affected in a differently manner regarding their phenotype, indicating that this pathogen reduce their growth in different ways. This study reports novel resistance sources with great potential that could be used in breeding programs to develop new pepper cultivars with durable resistance to P. capsici.

Incomplete Brachiocephalic Trunk in a Korean Water Deer (한국고라니의 불완전한 상완머리동맥)

  • Ahn, Dong-Choon;Tae, Hyun-Jin;Park, Byung-Yong;Sim, Jeoung-Ha;Kim, Jong-Taek;Kim, In-Shik
    • Journal of Veterinary Clinics
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    • v.28 no.5
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    • pp.526-529
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    • 2011
  • The brachiocephalic trunk (Bct) branches from the aortic arch (Aa) and consists, in ruminants, of the common trunk of the left subclavian artery (LSb), the bicarotid artery (Bc) or left and right common carotid artery (LCc and RCc), and the right subclavian artery (RSb). This pattern differs from the primitive mammalian Aa pattern due to the fact that the analogs of the LCc and LSb migrate cranially and merge with the common trunk of the RCc and RSb in the embryonic stage. A Bct having a septal remnant that consisted of the tunica media was observed in a female Korean water deer (Hydropotes inermis argyropus), which was deemed to have resulted from an incomplete merging of the vessel walls between a carnivoran-type Bct and an incomplete LSb. This is the first report of an abnormal Bct in a Korean water deer.

Differentiation of Human Mesenchymal Stem Cells towards Neuronal Lineage: Clinical Trials in Nervous System Disorders

  • Hernandez, Rosa;Jimenez-Luna, Cristina;Perales-Adan, Jesus;Perazzoli, Gloria;Melguizo, Consolacion;Prados, Jose
    • Biomolecules & Therapeutics
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    • v.28 no.1
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    • pp.34-44
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    • 2020
  • Mesenchymal stem cells (MSCs) have been proposed as an alternative therapy to be applied into several pathologies of the nervous system. These cells can be obtained from adipose tissue, umbilical cord blood and bone marrow, among other tissues, and have remarkable therapeutic properties. MSCs can be isolated with high yield, which adds to their ability to differentiate into non-mesodermal cell types including neuronal lineage both in vivo and in vitro. They are able to restore damaged neural tissue, thus being suitable for the treatment of neural injuries, and possess immunosuppressive activity, which may be useful for the treatment of neurological disorders of inflammatory etiology. Although the long-term safety of MSC-based therapies remains unclear, a large amount of both pre-clinical and clinical trials have shown functional improvements in animal models of nervous system diseases following transplantation of MSCs. In fact, there are several ongoing clinical trials evaluating the possible benefits this cell-based therapy could provide to patients with neurological damage, as well as their clinical limitations. In this review we focus on the potential of MSCs as a therapeutic tool to treat neurological disorders, summarizing the state of the art of this topic and the most recent clinical studies.

Effects on Nursing Students of Cognition-Behavior Integrated Breast Cancer Prevention Education Using an Interchangeable Nodule Model (결절교체 유방모형을 이용한 인지.행동 통합 유방암 예방교육 효과 -간호학생을 대상으로-)

  • Park, So-Mi;Kim, Bo-Hwan;Park, Mi-Jeong;Ahn, Yang-Heui;Chung, Chae-Weon
    • Women's Health Nursing
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    • v.16 no.2
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    • pp.166-176
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    • 2010
  • Purpose: The study was done to examine the effects of cognition-behavior integrated breast cancer prevention education, in which a breast model with interchangeable nodules was utilized, on the self-competency of nursing students in performing breast cancer education. Methods: A nonequivalent control group non-synchronized design was used. A traditional lecture intervention was provided for 49 3rd year college of nursing students (control group) while the integrated breast cancer prevention education was given to 47 3rd year students in the same college one year later (experimental group). The integrated breast cancer prevention education was developed by the research team to strengthen the competency of cognitive and behavioral components in education on breast cancer. Results: Effects of the intervention were found to be significant through all study variables: knowledge about breast cancer (t=7.79, p <.001), breast cancer risk awareness (t=2.05, p <.05), self-competency of breast self-exam (t=8.27, p <.001), and intention to teach breast self-exam (t=3.87, p <.001). Conclusion: The integrated breast cancer prevention education was useful to improve not only knowledge about breast cancer but competency in performing breast examination for nursing students who acquired technical skills from various simulation nodules. As the program helped the students to be prepared as confident educators, future application of the module is recommended for academic curricula.

New optimum distribution of lateral strength of shear-type buildings for uniform damage

  • Donaire-Avila, Jesus;Lucchini, Andrea;Benavent-Climent, Amadeo;Mollaioli, Fabrizio
    • Structural Engineering and Mechanics
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    • v.76 no.3
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    • pp.279-291
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    • 2020
  • The seismic design of conventional frame structures is meant to enhance plastic deformations at beam ends and prevent yielding in columns. To this end, columns are made stronger than beams. Yet yielding in columns cannot be avoided with the column-to-beam strength ratios (about 1.3) prescribed by seismic codes. Preventing plastic deformations in columns calls for ratios close to 4, which is not feasible for economic reasons. Furthermore, material properties and the rearrangement of geometric shapes inevitably make the distribution of damage among stories uneven. Damage in the i-th story can be characterized as the accumulated plastic strain energy (Wpi) normalized by the product of the story shear force (Qyi) and drift (δyi) at yielding. Past studies showed that the distribution of the plastic strain energy dissipation demand, Wpi/ΣWpj, can be evaluated from the deviation of Qyi with respect to an "optimum value" that would make the ratio Wpi/(Qyiδyi) -i.e. the damage- equal in all stories. This paper investigates how the soil type and ductility demand affect the optimum lateral strength distribution. New optimum lateral strength distributions are put forth and compared with others proposed in the literature.

Transvaluation of Values and Genealogy of Will to Power - Nietzsche's Criticism on Paul's Transvaluation of Values - (가치전도와 힘에의 의지의 계보학 - 바울의 가치전도에 대한 니체의 비판 -)

  • Chung, Nak-rim
    • Journal of Korean Philosophical Society
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    • v.148
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    • pp.327-356
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    • 2018
  • The aims this paper to examine Nietzsche's criticism on Paul's transvaluation of values. First, I will examine Socrates' transvaluation of values through which, Greek culture was deconstructed. He opened a path for the transvaluation of values before Paul and Nietzsche and reorganized Greek culture around his own values. I will also analyze the 'will to power' hidden behind Socrates' transvaluation of values. Second, I will examine the essence of the Paul's transvaluation of values. I will trace how Paul, through his transvaluation of values, could become the center of Christianity and, in turn, control European Culture. I will also show the difference between the teachings of Jesus and the arguments of Paul. Third, I will look at the Nietzsche's criticism on Paul's transvaluation of values. The key to Nietzsche's criticism on Paul is to evaluate Paul's transvaluation of values in terms of his will to power. And I will also look at the problems of Nietzsche's criticism on Paul. Fourth, I will look at Nietzsche's transvaluation of values. It is accomplished in such a way that he makes Socrates' and Paul's transvaluation of value alters. His transvaluation of values will be critically examined for strength in life and world understanding over Socrates' and Paul's transvaluation of values.

Development and testing of a composite system for bridge health monitoring utilising computer vision and deep learning

  • Lydon, Darragh;Taylor, S.E.;Lydon, Myra;Martinez del Rincon, Jesus;Hester, David
    • Smart Structures and Systems
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    • v.24 no.6
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    • pp.723-732
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    • 2019
  • Globally road transport networks are subjected to continuous levels of stress from increasing loading and environmental effects. As the most popular mean of transport in the UK the condition of this civil infrastructure is a key indicator of economic growth and productivity. Structural Health Monitoring (SHM) systems can provide a valuable insight to the true condition of our aging infrastructure. In particular, monitoring of the displacement of a bridge structure under live loading can provide an accurate descriptor of bridge condition. In the past B-WIM systems have been used to collect traffic data and hence provide an indicator of bridge condition, however the use of such systems can be restricted by bridge type, assess issues and cost limitations. This research provides a non-contact low cost AI based solution for vehicle classification and associated bridge displacement using computer vision methods. Convolutional neural networks (CNNs) have been adapted to develop the QUBYOLO vehicle classification method from recorded traffic images. This vehicle classification was then accurately related to the corresponding bridge response obtained under live loading using non-contact methods. The successful identification of multiple vehicle types during field testing has shown that QUBYOLO is suitable for the fine-grained vehicle classification required to identify applied load to a bridge structure. The process of displacement analysis and vehicle classification for the purposes of load identification which was used in this research adds to the body of knowledge on the monitoring of existing bridge structures, particularly long span bridges, and establishes the significant potential of computer vision and Deep Learning to provide dependable results on the real response of our infrastructure to existing and potential increased loading.

The Lived Experience of Elderly Women's dueitbaragi for Vietnamese Daughter-in-law (농촌여성노인의 베트남며느리 뒷바라지 체험)

  • Kim, Yun-Kyoung;Yang, Bok-Sun
    • Journal of Digital Convergence
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    • v.17 no.10
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    • pp.381-393
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    • 2019
  • This study aimed to explore and understand the meaning and essence of their experiences in the context of Vietnamese daughter-in-law and rural elderly women's lives. Van Manen's hermeneutics phenomenological method was used. Participants of the study were 8 people who live with a Vietnamese Daughter-in-law. Data was collected through individual in-depth interviews. 8 essential themes and 28 themes were derived from the data analysis. The rural elderly women who had Vietnamese Daughter-in-law had to continuously provide support for their son's family and were subject to a high level of stress due to the differences between Korean culture and Vietnamese culture. The findings can be used to develop a practical and comprehensive community nursing intervention based on the perspectives of all family members. For further study, exploratory study on the grandson who has a Vietnamese mother or the son who has a Vietnamese wife is recommanded.