• 생약학회지
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• 제52권3호
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• pp.134-142
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• 2021

This study was conducted to evaluate the effects of Dictyota coriacea extract and its active components such as 1,9-dihydroxycrenulide and epiloliolide on the hair growth. Treatment with D. coriacea extract and the hexane and EtOAc fractions of D. coriacea extract significantly increased the proliferation of dermal papilla cells (DPCs), a central regulator of the hair cycle. Especially, 1,9-dihydroxycrenulide and epiloliolide from D. coriacea extract, caused an increase in the DPC proliferation. When isolated rat vibrissa follicles were treated with 1,9-dihydroxycrenulide or epiloliolide for 21 d, the hair-fiber lengths for the vibrissa follicles increased. When examined the activity of 5α-reductase, which converts testosterone to dihydrotestosterone (DHT), a main cause of androgenetic alopecia, the several solvent fractions of D. coriacea extract significantly decreased the 5α-reductase activity while 1,9-dihydroxycrenulide and epiloliolide scarcely inhibited 5α-reductase activity. In addition, we found that the D. coriacea extract and several solvent fractions of D. coriacea extract could not act as a K_ATP channel opener, which could be a contributory factor in the effect on hair growth. These results suggest that D. coriacea extract and 1,9-dihydroxycrenulide and epiloliolide, principals of D. coriacea, have the potential to treat alopecia via the proliferation of DPCs.

• ALGAE
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• 제34권2호
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• pp.177-185
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• 2019

Ecklonia cava is popular in Korea as a marine functional materials. E. cava is generally collected and used on the coast of Jeju Island. However, the continuous use of collected natural E. cava may be limited because difficult to secure throughout the year and may be exposed to environmental pollution. Jeju magma seawater (MSW) was known to be significant advantages such as safety, cleanness, stability, and functional improvement. Attempts have been reported on application of MSW to the culturing of macro- and microalgae and showed improved results. Thus, the objective of the present study was to explore the anti-melanogenesis activity of brown seaweed E. cava (E. cava cultured with MSW [MSWE]) extract cultured in tanks with MSW of Jeju Island to evaluate the possibility of cosmeceutical industrial application. MSWE extract showed the higher polyphenolic and dieckol contents than natural E. cava (NE) extract. Anti-melanogenesis activity of MSWE extract and NE extract are tested and compared using tyrosinase and dihydroxyphenylalanine (DOPA) oxidation inhibition assay. MSWE extracts evidenced more effective tyrosinase and DOPA oxidation inhibition activity than that of the NE extracts and the commercial whitening agent, arbutin. MSWE extracts also markedly inhibited melanin synthesis and decreased the expression of melanogenesis-related protein in ${\alpha}$-melanocyte stimulating hormone-stimulated B16F10 melanoma cells without cytotoxicity. These results suggest that MSW cultivation process would be more effective in releasing bioactive compounds with whitening effect from seaweed such as E. cava at an industrial scale.

• 한국의류학회지
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• 제46권1호
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• pp.132-146
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• 2022

To explore the potential use of Ecklonia cava as a natural dye for textiles, silk fabric was dyed with Ecklonia cava extracted by hot water under varied conditions, including temperature, duration, dye concentration, mordanting, and pH adjustment. The fabric was also evaluated for fastness, sun-protective property, and deodorization. Ecklonia cava extract was estimated by FT-IR to have polyphenol as a main functional colorant in plants, while the existence of phlorotannins through the UV-spectrum method was also confirmed. The fabric was optimized for maximum dye uptake at a temperature of 80℃ for a duration of 50 minutes along all dye concentrations. The dyed fabric showed a hue of Yellow Red under all dyeing conditions while additional color tones, such as grayish and dark, were generated through mordanting and pH adjustment. Both good sun-protective properties and a positive deodorization rate were also recorded, with more than 20% (owf) Ecklonia cava extract on silk fabric. These results imply that Ecklonia cava has great potential to be used as an eco-friendly natural dye and in fashion goods with skin-health functions made of silk.

• 대한화장품학회지
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• 제44권1호
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• pp.59-66
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• 2018

Propionibacterium acnes (P. acnes)는 여드름의 주요 병원성균으로 염증성 질환에 관련된 미생물 중에 하나이다. 본 연구에서는 여드름 주요 유발 균주인 P. acnes에 대하여 70% Ethanol로 추출한 제주 자생식물 추출물의 항균활성을 disc diffusion test 실험을 이용하여 측정하였다. 실험 결과 61개의 제주 자생 식물 추출물 중에서 45개 추출물의 항균 활성이 검출되었으며, 16개의 추출물은 항균 활성이 검출되지 않았다. 그중에서 여우 구슬(Phyllanthus urinaria L.) - 줄기 잎 추출물이 $18.96{\pm}0.69mm$로 가장 높은 항균 활성을 나타내었고, 참당귀(Angelica gigas Nakai) - 뿌리, 돌외(Gynostemma pentaphyllum (Thunb.) Makino), 참당귀(Angelica gigas Nakai) - 잎 줄기, 산박하(Isodon inflexus (Thunb.) Kudo), 차나무(Camellia sinensis L.) - 꽃, 그리고 로즈마리(Rosmarinus officinalis L.) - 줄기 추출물 순으로 높은 항균 활성을 나타내었다. 이러한 결과는 제주 자생 식물 추출물이 여드름 치료 예방의 항균제 및 기능성 화장품 천연 원료로써의 이용 가능성을 제안한다.

• 한국식품영양과학회지
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• 제36권3호
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• pp.255-259
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• 2007

The solvent extracts of Oenothera laciniata, which were extracted by using several solvents with different polarities, were prepared for utility as a natural preservative. The O. laciniata extract by 80% ethanol was sequentially fractionated with n-hexane, dichloromethane, ethylacetate, and butanol. The antibacterial activities and cell growth inhibition were investigated on each strain with the different concentrations of O. laciniata extracts. Antibacterial activities were shown in ethanol, ethylacetate, and butanol fraction of O. laciniata. However n-hexane, dichloromethane and water fraction showed weak antibacterial activity against the tested microorganisms. Among the five fractions, ethylacetate fraction showed the highest antibacterial activities against microorganisms tested, such as Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella Enteritidis and Salmonella Typhimurium. The polyphenolic compounds widely occurring in the traditional medicine plants have been reported to possess high antibacterial activity. The polyphenolic compounds from ethanol, n-hexane, dichloromethane, ethylacetate, butanol, and water fraction were 63.96 mg/g, 8.49 mg/g, 28.11 mg/g, 172.64 mg/g, 114.56 mg/g, and 34.91 mg/g, respectively. There are some relationships between antibacterial activity and polyphenol content in natural plant. The ethylacetate fraction could be suitable for the development of a food preservative.

• 한국식품조리과학회지
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• 제31권5호
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• pp.515-523
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• 2015

In the study reported here, the antimicrobial and antioxidant activities of the ethanol leaf extract of Dendropanax morbiferus Lev. from Jeju Island was investigated. Of the 14 strains of 12 species of microorganisms tested, the extract exhibited antimicrobial activity observed against seven Gram-positive bacteria of four species, but not against six Gram-negative bacteria and a yeast strain. Using the disc diffusion method, the diameter of the inhibition zone increased with application of the extract with every strain and the highest growth inhibition was exhibited with Staphylococcus aureus KCTC 1916 at 5 mg/ml. The minimal inhibitory concentration of the extract (MIC) by turbidity was 2.5 mg/ml against Bacillus cereus KACC 12672 and 15 mg/ml when with Enterococcus faecalis KCTC 3206. The minimum bacterial concentration (MBC) values defined as being $${\geq_-}99.9%$$ reduction in viable cells against the tested strains was higher than the MIC values. Time killing curves using the optimum MIC were performed on seven strains incubated for 48 hr. The growth of B. cereus KACC 12672 was detected after 12 hr and no significant growth was found in the others strains after 48 hr (p<0.05). The 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity of the ethanol leaf extract was similar to that of butylated hydroxyanisole (BHA) at the same concentration. These results indicate that leaf extract of D. morbiferus Lev. can be utilized as a natural preservative and an antioxidant.

• 대한화학회지
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• 제67권2호
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• pp.150-155
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• 2023

Eurya emarginata is an evergreen tree growing in the southern regions of Korea including Jeju Island. In this study, antioxidant and anti-photoaging effects were investigated with the extracts of E. emarginata leaves. As comparative studies with the extract and five solvent fractions, the EtOAc fraction showed the most effective DPPH and ABTS⁺ radical scavenging activities. The EtOAc fraction also inhibited matrix metalloproteinase-1 (MMP-1) production most effectively. Phytochemical study on the EtOAC fraction resulted in the isolation of three compounds; eutigoside C (1), eutigoside B (2) and quercitrin (3). Among the isolates (1-3), quercitrin (3) showed excellent antioxidant activities and inhibitory properties on MMP-1 production. Based on these results, it was suggested that extracts of E. emarginata leaves could be developed as natural anti-photoaging agents in cosmetic formulations.

• KSBB Journal
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• 제23권1호
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• pp.77-82
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• 2008

본 연구는 제주도의 자생식물인 노루참나물 (Pimpinella komarovii)의 산업적 활용 가능성을 평가하기 위하여 에탄올 추출물 및 순차적 용매분획물을 얻어 항산화 활성 및 항염증 효과를 검색하고자 하였으며, 마우스 유래의 melan-a 세포에 처리하여 멜라닌 생합성 및 tyrosinas 억제활성을 통하여 유용자원으로서 미백 및 기능성 화장료로 활용가능성이 있는지 알아보고자 연구를 시행하였다. 노루참나물 에탄올 추출물은 높은 항산화 활성 (DPPH 소거활성 $IC_{50}$ 값, $231.8{\mu}g/m{\ell}$ ; superoxide 소거 활성 및 xanthine oxidase 억제 활성 $IC_{50}$ 값, 각각 $23.6{\mu}g/m{\ell},\;587.8{\mu}g/m{\ell}$)을 나타내었으며, 순차적 분획물 중에서는 에틸아세테이트 분획물이 가장 높은 항산화 활성을 나타내었다. 그리고 멜라닌 생성 세포인 melan-a 세포에서 노루참나물 에탄올 추출물과 에틸아세테이트 분획물이 tyrosinase와 멜라닌 합성을 농도 의존적으로 억제하였다. 또한 LPS로 자극한 RAW 264.7 세포에서 노루참나물의 헥산 분획물과 에틸아세테이트 분획물이 가장 높은 NO생성 억제 활성을 보였다. 본 연구결과는 노루참나물 추출물이 항산화 및 항염증 활성을 가지는 미백 관련 기능성 소재로서의 활용가치가 있음을 보여준다.

• Toxicological Research
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• 제27권3호
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• pp.173-180
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• 2011

Atopic dermatitis (AD) is a chronic, recurrent inflammatory skin disease that is associated with Th2 cell-mediated allergy. The process that leads to infiltration of inflammatory cells into an AD lesion is remarkably dependent on various chemokines, especially TARC (thymus and activation-regulated chemokine/CCL17) and MDC (macrophage-derived chemokine/CCL22). Serum levels of these chemokines are over-expressed in AD patients. Citrus unshiu, which is known as Satsuma mandarin, has anti-oxidative, anti-inflammation, and anti-microviral activity. Therefore, we investigated the effect of EtOH extract of premature C. unshiu on AD. We did this using a DNCB-induced AD mouse model. We also tried to confirm an inhibitory effect for premature C. unshiu on the expression of inflammatory chemokines in IFN-${\gamma}$ and TNF-${\alpha}$ stimulated HaCaT human keratinocytes. We found that extract of premature C. unshiu reduced DNCB-induced symptoms such as hyperkeratosis, increased skin thickness, and infiltrated mast cells, in our AD-like animal model. The extract decreased levels of IFN-${\gamma}$ and IL-4 in ConA-stimulated splenocytes isolated from DNCB-treated mice. Also, extract of premature C. unshiu inhibited mRNA expression and protein production of TARC and MDC through the inhibition of STAT1 phosphorylation. These results suggest that C. unshiu has anti-atopic activity by regulating inflammatory chemokines such as TARC and MDC.

• 한국염색가공학회지
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• 제22권3호
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• pp.194-206
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• 2010

This study was carried out to identify the optimum dyeing conditions for maximum dye uptake of Prunus yedoensis Matsumura flower extract on silk fabric and to investigate hue/tone characteristics and fastness properties of the dyed fabrics. As results, the flower extract was found to contain flavonoids by FT-IR and to show yellowish shade by UV-vis spectrum. Silk fabric was optimized for maximum dye uptake to five repetitions of dyeing at $80^{\circ}C$ for 60 min with an extract concentration of 600% owf in terms of both K/S and color differences. Pre-mordanting was more effective to dye uptake than post-mordanting for all of the mordants used. Un-mordanted and pre-mordanted fabric showed YR(Yellow Red) while most of post-mordanted ones did Y(Yellow). Main tones of dyed fabrics were sf(soft) and lt(light) in the case of unmordanting or Al-mordanting whereas they were d(dull) and g(grayish) by post-mordanting with Cu, Cr, and Fe, respectively. The fastness grades to rub and dry cleaning were reasonably good for most dyeing conditions while those to light were poor. Finally dyeing condition for each hue/tone of the dyed silk with the flower extract was optimized considering fastness properties.