KSBB Journal

  • 제23권1호
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  • Pages.77-82
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  • 2008
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  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

제주도 자생 노루참나물 (Pimpinella komarovii) 추출물의 항산화 효과 및 멜라닌 억제 효과

Antioxidant Effects and Melanin Inhibitory Effect of Natural Pimpinella komarovii Extracts in Jeju Island

  • 강민철 ((재)제주하이테크산업진흥원 생물자원산업화지원센터) ;
  • 이주엽 ((재)제주하이테크산업진흥원 생물자원산업화지원센터) ;
  • 이정아 ((재)제주하이테크산업진흥원 생물자원산업화지원센터) ;
  • 한종헌 ((재)제주하이테크산업진흥원 생물자원산업화지원센터) ;
  • 김봉석 ((재)제주하이테크산업진흥원 생물자원산업화지원센터) ;
  • 김기옥 ((재)제주하이테크산업진흥원 생물자원산업화지원센터)
  • Kang, Min-Chul (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute) ;
  • Lee, Ju-Yeop (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute) ;
  • Lee, Jung-A (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute) ;
  • Han, Jong-Heon (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute) ;
  • Kim, Bong-Seok (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute) ;
  • Kim, Gi-Ok (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute)
  • 발행 : 2008.02.29
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초록

본 연구는 제주도의 자생식물인 노루참나물 (Pimpinella komarovii)의 산업적 활용 가능성을 평가하기 위하여 에탄올 추출물 및 순차적 용매분획물을 얻어 항산화 활성 및 항염증 효과를 검색하고자 하였으며, 마우스 유래의 melan-a 세포에 처리하여 멜라닌 생합성 및 tyrosinas 억제활성을 통하여 유용자원으로서 미백 및 기능성 화장료로 활용가능성이 있는지 알아보고자 연구를 시행하였다. 노루참나물 에탄올 추출물은 높은 항산화 활성 (DPPH 소거활성 $IC_{50}$ 값, $231.8{\mu}g/m{\ell}$ ; superoxide 소거 활성 및 xanthine oxidase 억제 활성 $IC_{50}$ 값, 각각 $23.6{\mu}g/m{\ell},\;587.8{\mu}g/m{\ell}$)을 나타내었으며, 순차적 분획물 중에서는 에틸아세테이트 분획물이 가장 높은 항산화 활성을 나타내었다. 그리고 멜라닌 생성 세포인 melan-a 세포에서 노루참나물 에탄올 추출물과 에틸아세테이트 분획물이 tyrosinase와 멜라닌 합성을 농도 의존적으로 억제하였다. 또한 LPS로 자극한 RAW 264.7 세포에서 노루참나물의 헥산 분획물과 에틸아세테이트 분획물이 가장 높은 NO생성 억제 활성을 보였다. 본 연구결과는 노루참나물 추출물이 항산화 및 항염증 활성을 가지는 미백 관련 기능성 소재로서의 활용가치가 있음을 보여준다.

We investigated several biological activities using the ethanol extract and its fractions from Pimpinella komarovii leaves to evaluate the usefulness of its extract as a functional biomaterial. The ethanol extract showed antioxidant activities, such as DPPH scavenging activity $(IC_{50}=231.8{\mu}g/m{\ell})$. superoxide scavenging activity $(IC_{50}=23.6{\mu}g/m{\ell})$, and xanthine oxidase inhibitory activity $(IC_{50}=587.8{\mu}g/m{\ell})$. Its EtOAc fraction showed the strongest antioxidant activities among several fractions. The inhibitory effect of ethanol extract on tyrosinase activity was higher than water fraction. When $50{\mu}g/m{\ell}$ of EtOAc fraction was applied, the inhibition ratio of tyrosinase activity was much higher (42%) than that of melasolv. The EtOAc fraction also showed higher inhibitory effect on melanogenesis in Melan-a cells. The n-hexane and EtOAc fractions dose-dependently inhibited the NO production in a RAW 264.7 cells. These results suggest that extract of Pimpinella komarovii could be used as functional biomaterial in developing a skin whitening agent having the antioxidant activity.

키워드

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