KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antioxidant Effects and Melanin Inhibitory Effect of Natural Pimpinella komarovii Extracts in Jeju Island

제주도 자생 노루참나물 (Pimpinella komarovii) 추출물의 항산화 효과 및 멜라닌 억제 효과

  • Kang, Min-Chul (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute) ;
  • Lee, Ju-Yeop (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute) ;
  • Lee, Jung-A (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute) ;
  • Han, Jong-Heon (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute) ;
  • Kim, Bong-Seok (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute) ;
  • Kim, Gi-Ok (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute)
  • 강민철 ((재)제주하이테크산업진흥원 생물자원산업화지원센터) ;
  • 이주엽 ((재)제주하이테크산업진흥원 생물자원산업화지원센터) ;
  • 이정아 ((재)제주하이테크산업진흥원 생물자원산업화지원센터) ;
  • 한종헌 ((재)제주하이테크산업진흥원 생물자원산업화지원센터) ;
  • 김봉석 ((재)제주하이테크산업진흥원 생물자원산업화지원센터) ;
  • 김기옥 ((재)제주하이테크산업진흥원 생물자원산업화지원센터)
  • Published : 2008.02.29
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Abstract

We investigated several biological activities using the ethanol extract and its fractions from Pimpinella komarovii leaves to evaluate the usefulness of its extract as a functional biomaterial. The ethanol extract showed antioxidant activities, such as DPPH scavenging activity $(IC_{50}=231.8{\mu}g/m{\ell})$. superoxide scavenging activity $(IC_{50}=23.6{\mu}g/m{\ell})$, and xanthine oxidase inhibitory activity $(IC_{50}=587.8{\mu}g/m{\ell})$. Its EtOAc fraction showed the strongest antioxidant activities among several fractions. The inhibitory effect of ethanol extract on tyrosinase activity was higher than water fraction. When $50{\mu}g/m{\ell}$ of EtOAc fraction was applied, the inhibition ratio of tyrosinase activity was much higher (42%) than that of melasolv. The EtOAc fraction also showed higher inhibitory effect on melanogenesis in Melan-a cells. The n-hexane and EtOAc fractions dose-dependently inhibited the NO production in a RAW 264.7 cells. These results suggest that extract of Pimpinella komarovii could be used as functional biomaterial in developing a skin whitening agent having the antioxidant activity.

본 연구는 제주도의 자생식물인 노루참나물 (Pimpinella komarovii)의 산업적 활용 가능성을 평가하기 위하여 에탄올 추출물 및 순차적 용매분획물을 얻어 항산화 활성 및 항염증 효과를 검색하고자 하였으며, 마우스 유래의 melan-a 세포에 처리하여 멜라닌 생합성 및 tyrosinas 억제활성을 통하여 유용자원으로서 미백 및 기능성 화장료로 활용가능성이 있는지 알아보고자 연구를 시행하였다. 노루참나물 에탄올 추출물은 높은 항산화 활성 (DPPH 소거활성 $IC_{50}$ 값, $231.8{\mu}g/m{\ell}$ ; superoxide 소거 활성 및 xanthine oxidase 억제 활성 $IC_{50}$ 값, 각각 $23.6{\mu}g/m{\ell},\;587.8{\mu}g/m{\ell}$)을 나타내었으며, 순차적 분획물 중에서는 에틸아세테이트 분획물이 가장 높은 항산화 활성을 나타내었다. 그리고 멜라닌 생성 세포인 melan-a 세포에서 노루참나물 에탄올 추출물과 에틸아세테이트 분획물이 tyrosinase와 멜라닌 합성을 농도 의존적으로 억제하였다. 또한 LPS로 자극한 RAW 264.7 세포에서 노루참나물의 헥산 분획물과 에틸아세테이트 분획물이 가장 높은 NO생성 억제 활성을 보였다. 본 연구결과는 노루참나물 추출물이 항산화 및 항염증 활성을 가지는 미백 관련 기능성 소재로서의 활용가치가 있음을 보여준다.

Keywords

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